##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003778_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4799673 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786100219744135 31.0 31.0 34.0 30.0 34.0 2 31.96335750372994 31.0 31.0 34.0 30.0 34.0 3 32.03119795869427 33.0 31.0 34.0 30.0 34.0 4 35.714835989868476 37.0 35.0 37.0 35.0 37.0 5 35.599922119694405 37.0 35.0 37.0 33.0 37.0 6 35.69696185552641 37.0 35.0 37.0 33.0 37.0 7 35.66063750593009 37.0 35.0 37.0 33.0 37.0 8 35.68009653991012 37.0 35.0 37.0 33.0 37.0 9 37.26185492219991 39.0 37.0 39.0 33.0 39.0 10-11 37.16089596103735 39.0 37.0 39.0 33.0 39.0 12-13 37.148811179428264 39.0 37.0 39.0 33.0 39.0 14-15 38.18512334069425 40.0 37.0 41.0 33.0 41.0 16-17 38.2580625805133 40.0 37.5 41.0 33.0 41.0 18-19 38.335030219767056 40.0 38.0 41.0 33.5 41.0 20-21 38.26303416920278 40.0 38.0 41.0 33.5 41.0 22-23 38.17200911395422 40.0 37.5 41.0 33.0 41.0 24-25 38.00102079870858 40.0 37.0 41.0 33.0 41.0 26-27 37.77400689588645 39.5 37.0 41.0 32.5 41.0 28-29 37.69958213819983 39.0 37.0 41.0 33.0 41.0 30-31 37.57436464942508 39.0 37.0 41.0 32.0 41.0 32-33 37.329485779552066 39.0 36.0 41.0 31.5 41.0 34-35 37.24472958886991 39.0 36.0 41.0 31.0 41.0 36-37 37.09313842838877 39.0 35.5 41.0 30.5 41.0 38-39 36.851552387006365 39.0 35.0 40.0 30.5 41.0 40-41 37.03736858740169 39.0 36.0 41.0 31.0 41.0 42-43 36.926367796306124 39.0 36.0 41.0 31.0 41.0 44-45 36.67295094895006 39.0 35.5 41.0 30.0 41.0 46-47 36.52994016467372 39.0 35.0 41.0 30.0 41.0 48-49 36.2313124248256 39.0 35.0 41.0 29.0 41.0 50-51 35.97284565010991 39.0 35.0 40.0 28.0 41.0 52-53 35.89496117756356 39.0 35.0 40.0 28.0 41.0 54-55 35.746976304427406 39.0 35.0 40.0 28.0 41.0 56-57 35.36687874361441 38.0 34.5 40.0 27.5 41.0 58-59 35.06527465516922 38.0 34.0 40.0 26.5 41.0 60-61 34.52585436549532 38.0 33.5 40.0 26.0 41.0 62-63 34.37140144755695 37.0 33.0 40.0 26.0 41.0 64-65 33.96662272617322 37.0 33.0 39.5 25.5 41.0 66-67 33.48997723386572 36.0 32.5 39.0 24.5 40.0 68-69 33.060569542966775 36.0 32.0 39.0 23.5 40.0 70-71 32.48592529116046 35.0 31.5 37.5 22.5 40.0 72-73 32.01483569818194 35.0 31.0 37.0 21.5 39.0 74-75 31.706989934522625 35.0 31.0 37.0 21.0 39.0 76-77 30.724113288551116 34.0 29.5 35.5 20.0 38.0 78-79 31.152410070436048 34.0 31.0 36.0 20.0 37.5 80-81 30.84235217690872 34.0 31.0 35.5 20.0 37.0 82-83 30.51382854623638 34.0 30.5 35.0 19.0 37.0 84-85 30.163494367220434 34.0 30.0 35.0 18.0 36.0 86-87 30.09697160619067 34.0 30.0 35.0 17.5 36.0 88-89 29.77404616939529 34.0 30.0 35.0 11.0 36.0 90-91 29.36826404632149 34.0 29.0 35.0 7.0 35.0 92-93 29.143690934778267 34.0 29.0 35.0 2.0 35.0 94-95 28.731351906681976 34.0 29.0 35.0 2.0 35.0 96-97 28.349987176209712 34.0 29.0 35.0 2.0 35.0 98-99 28.118993627274193 34.0 29.0 35.0 2.0 35.0 100-101 27.1172495501256 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.04869532320215697 1101 2 0.08380566435606696 1101 3 0.1555888657505875 1101 4 0.07572368208648328 1101 5 0.11646075449217363 1101 6 0.154011978581444 1101 7 0.1416133929211938 1101 8 0.07170439611555679 1101 9 0.1371630783774478 1101 10-11 0.21381537774237103 1101 12-13 0.178013137188195 1101 14-15 0.25549267635431505 1101 16-17 0.26406652601868785 1101 18-19 0.24183574853022094 1101 20-21 0.10091359555292456 1101 22-23 0.13226780048090347 1101 24-25 0.22176497840844434 1101 26-27 0.20902183644957262 1101 28-29 0.22323457327203045 1101 30-31 0.2832632308279557 1101 32-33 0.2494968310744028 1101 34-35 0.19242205673167945 1101 36-37 0.24422290488682563 1101 38-39 0.14721071661856655 1101 40-41 0.2640552149213633 1101 42-43 0.24940676521591598 1101 44-45 0.31409365360465813 1101 46-47 0.2901639819093589 1101 48-49 0.22767961006681503 1101 50-51 0.1959415619517486 1101 52-53 0.2347656076019291 1101 54-55 0.32514327306198254 1101 56-57 0.26235745624775575 1101 58-59 0.27167105559798443 1101 60-61 0.17516999567845204 1101 62-63 0.2052584184893007 1101 64-65 0.1033440602615272 1101 66-67 0.035478032254204095 1101 68-69 0.11623506782345316 1101 70-71 0.18933775767209227 1101 72-73 0.23488820893929585 1101 74-75 0.07636296367251916 1101 76-77 0.16603368569948174 1101 78-79 0.31859789507506875 1101 80-81 0.20855738424684844 1101 82-83 0.2568791249687017 1101 84-85 0.24379436200665694 1101 86-87 0.051496309890875835 1101 88-89 0.11635142234935003 1101 90-91 0.14112914625492579 1101 92-93 0.20691953897815551 1101 94-95 0.37411589988783334 1101 96-97 0.34121007081096977 1101 98-99 0.25730597938569133 1101 100-101 0.36000289895464377 1102 1 0.011948751849459427 1102 2 0.1181512117530481 1102 3 0.12472070397154766 1102 4 0.07708243178100815 1102 5 0.04459878610755652 1102 6 -0.026616335771080912 1102 7 0.01153096731037806 1102 8 0.02414270439196997 1102 9 0.019479797796556397 1102 10-11 0.0996717344542688 1102 12-13 0.06229090204318055 1102 14-15 0.08721110108490393 1102 16-17 0.08715434638239827 1102 18-19 0.05016250823836543 1102 20-21 0.02256291974764224 1102 22-23 0.14189856998926587 1102 24-25 -0.00627398792347833 1102 26-27 0.012280635137379647 1102 28-29 0.03314270988366985 1102 30-31 0.05146374555162936 1102 32-33 0.05172294626000706 1102 34-35 0.12635035708051845 1102 36-37 0.07749648393448183 1102 38-39 0.12803282706409647 1102 40-41 0.13557905511617463 1102 42-43 0.28195968404278915 1102 44-45 0.31185524106552265 1102 46-47 0.05839307277993555 1102 48-49 0.12823301058907077 1102 50-51 0.2710513457419168 1102 52-53 0.16306217112903454 1102 54-55 0.12956896703870768 1102 56-57 0.19002601138520703 1102 58-59 0.2098313715085638 1102 60-61 0.0878230310457937 1102 62-63 -0.03979875145619616 1102 64-65 -0.18991736264622716 1102 66-67 -0.10923592973343688 1102 68-69 -0.03179502166284465 1102 70-71 0.06312013334820676 1102 72-73 0.0818018477830833 1102 74-75 -0.033725769480401624 1102 76-77 -0.053469995465249553 1102 78-79 0.04478802196728182 1102 80-81 0.09382435057406369 1102 82-83 0.061381015466160704 1102 84-85 0.08190676334834635 1102 86-87 0.08208135795646498 1102 88-89 0.06318964919256942 1102 90-91 0.15677139956942554 1102 92-93 0.20474190713856544 1102 94-95 0.21938683196446718 1102 96-97 0.3293596976343416 1102 98-99 0.17341545368265088 1102 100-101 0.30780651708318274 1103 1 -0.0973408163625642 1103 2 -0.005910546592595267 1103 3 -0.05736257377631304 1103 4 -0.017041565827575766 1103 5 0.06353671648324877 1103 6 -0.0036912386406058317 1103 7 0.10658880090969802 1103 8 -0.003641510321131136 1103 9 0.04427573733931922 1103 10-11 0.0029490335107169585 1103 12-13 0.02637327642413112 1103 14-15 0.046727602148024516 1103 16-17 0.07884732350753865 1103 18-19 -0.10442360231002823 1103 20-21 -0.08932695760196907 1103 22-23 -0.0033302198284630435 1103 24-25 0.05112390361649943 1103 26-27 -0.11834270038642813 1103 28-29 -0.05718704374503858 1103 30-31 -0.007992043819768924 1103 32-33 0.02929774784045236 1103 34-35 0.08498179326098665 1103 36-37 0.003994368392454817 1103 38-39 -0.01894381715427329 1103 40-41 -0.0919337377781062 1103 42-43 0.16065465648062371 1103 44-45 0.15087995066228643 1103 46-47 0.07326410212413492 1103 48-49 0.1205957926956529 1103 50-51 0.28299444103765126 1103 52-53 0.20694029565038363 1103 54-55 0.20659530467229814 1103 56-57 0.12591493842906942 1103 58-59 0.19152546857809938 1103 60-61 0.10600676945313836 1103 62-63 -0.007477732570308149 1103 64-65 0.09306656450468864 1103 66-67 0.17478207254927725 1103 68-69 0.06282265731827152 1103 70-71 0.07373387111322671 1103 72-73 0.3280526074512551 1103 74-75 0.08241439979590126 1103 76-77 0.10168535152811131 1103 78-79 0.17783941341584608 1103 80-81 0.21058050985650922 1103 82-83 0.24182783622400095 1103 84-85 0.13101282537679637 1103 86-87 0.15363888768851908 1103 88-89 0.07651093488874139 1103 90-91 0.21552411502284485 1103 92-93 0.24940638085528022 1103 94-95 0.2570484210008743 1103 96-97 0.33808044114566727 1103 98-99 0.3749144086757106 1103 100-101 0.5708618983336642 1104 1 0.06989665897140185 1104 2 0.05917820380372163 1104 3 0.13868920478068247 1104 4 0.08354261100125626 1104 5 -0.016080741152691758 1104 6 0.03821380718970602 1104 7 0.04126107983816496 1104 8 0.021956152971547738 1104 9 0.058101137319447105 1104 10-11 0.13245664744767538 1104 12-13 0.14458775142211522 1104 14-15 0.18465656634027994 1104 16-17 0.23723992630041835 1104 18-19 0.1016327072256118 1104 20-21 0.06553520837144333 1104 22-23 0.08707167679423833 1104 24-25 0.2180673976990164 1104 26-27 0.21366653429223703 1104 28-29 0.19976232996251753 1104 30-31 0.15723961577241852 1104 32-33 0.30056081433108517 1104 34-35 0.2289444712468125 1104 36-37 0.18804674319311232 1104 38-39 0.40911299634922926 1104 40-41 0.2986443109908379 1104 42-43 0.22568218542919993 1104 44-45 0.4500061697676401 1104 46-47 0.433985308523809 1104 48-49 0.25056478565902296 1104 50-51 0.29579061088191594 1104 52-53 0.22785052548811535 1104 54-55 0.35294029551570105 1104 56-57 0.28770843700706905 1104 58-59 0.30303013205533347 1104 60-61 0.2394606528449117 1104 62-63 0.11678346947584117 1104 64-65 0.3445110357643202 1104 66-67 0.34830228804877095 1104 68-69 0.22149327055014822 1104 70-71 0.16984799498994363 1104 72-73 0.17361041469419547 1104 74-75 0.16866920878498348 1104 76-77 0.094060471604962 1104 78-79 0.2395024245409978 1104 80-81 0.19714935233934483 1104 82-83 0.2108559945408892 1104 84-85 0.30796410375160477 1104 86-87 0.23546268348925992 1104 88-89 0.10477868141893154 1104 90-91 0.1573942025192352 1104 92-93 0.22219122916805034 1104 94-95 0.3018151998181331 1104 96-97 0.40318811991229353 1104 98-99 0.3652662634193966 1104 100-101 0.599350870191909 1105 1 -0.08104540769494051 1105 2 -0.07342820609577316 1105 3 -9.171811765895654E-5 1105 4 0.025563456643119764 1105 5 -0.10440543566399896 1105 6 -0.007160836051006925 1105 7 -0.026155490038057394 1105 8 0.002369712566576254 1105 9 -0.06258156335265852 1105 10-11 -0.03696691251530382 1105 12-13 -0.028875493433297095 1105 14-15 0.03955373184395938 1105 16-17 0.03687433264811801 1105 18-19 -0.08711213187083189 1105 20-21 -0.09170572937002675 1105 22-23 0.019617096563933956 1105 24-25 0.07487527774874536 1105 26-27 -0.03911013075219216 1105 28-29 -0.0673911006541843 1105 30-31 -0.013607987353665862 1105 32-33 0.007554268219145399 1105 34-35 -0.031173225471526678 1105 36-37 0.010381141279196981 1105 38-39 0.03842187785897977 1105 40-41 0.12227375134651908 1105 42-43 0.12023786174621875 1105 44-45 0.13696556360142154 1105 46-47 0.05315002491797571 1105 48-49 -0.0017416097305797962 1105 50-51 0.0556390675921179 1105 52-53 0.07696382550193448 1105 54-55 0.020959728699743607 1105 56-57 0.049684518948367895 1105 58-59 0.13569970162539136 1105 60-61 0.07355717276268336 1105 62-63 0.02142490687122489 1105 64-65 -0.10566255353085552 1105 66-67 -0.06922487633715946 1105 68-69 -0.17609273351678212 1105 70-71 -0.040544782140244706 1105 72-73 -0.09604493032527017 1105 74-75 -0.07246830399795101 1105 76-77 0.012771033693052658 1105 78-79 -0.017436721402798838 1105 80-81 -0.008159192804807702 1105 82-83 0.19003256877701702 1105 84-85 0.06359762006689706 1105 86-87 0.017030239170086503 1105 88-89 0.12173442348170838 1105 90-91 0.10444282869660526 1105 92-93 0.17927215893949366 1105 94-95 0.10762746025744363 1105 96-97 0.2872571040187566 1105 98-99 0.2961916871046739 1105 100-101 0.2898471795427362 1106 1 -0.05501153603402997 1106 2 0.003842600285334896 1106 3 0.007207624073465979 1106 4 -0.09373641236861374 1106 5 0.012666888386924313 1106 6 -0.022676677248966826 1106 7 -0.017553801712189454 1106 8 -0.01750471442869639 1106 9 -0.023576250662749487 1106 10-11 -0.0016886580377857285 1106 12-13 -0.08752801186006565 1106 14-15 -0.04728359731156928 1106 16-17 -0.035165720423641744 1106 18-19 -0.07084824584643457 1106 20-21 0.054391542743296384 1106 22-23 0.04337223012380065 1106 24-25 0.013607566484679978 1106 26-27 0.0025851654713520134 1106 28-29 -0.07095862835821976 1106 30-31 7.623498887312508E-4 1106 32-33 0.09200374068164052 1106 34-35 0.13740707202587288 1106 36-37 0.15811057386068228 1106 38-39 0.17096399685426178 1106 40-41 0.16371928570547567 1106 42-43 0.1030783485166964 1106 44-45 0.19864263616995714 1106 46-47 0.14236983410818027 1106 48-49 0.22967688876818215 1106 50-51 0.14148709161043627 1106 52-53 0.1498276561605465 1106 54-55 0.2832253799224489 1106 56-57 0.1659574901467593 1106 58-59 0.2202876804834446 1106 60-61 0.11807356026589133 1106 62-63 0.05789898531198645 1106 64-65 0.09590710443279704 1106 66-67 0.035349887889481124 1106 68-69 0.0574616080475181 1106 70-71 0.14973106708685435 1106 72-73 0.3111360242824972 1106 74-75 0.3322021647473967 1106 76-77 0.1789864976242228 1106 78-79 0.2371652845595058 1106 80-81 0.26249211536833883 1106 82-83 0.22882612384558243 1106 84-85 0.23148860239065172 1106 86-87 0.35029714549785496 1106 88-89 0.1884661982642477 1106 90-91 0.1565125021009095 1106 92-93 0.1373475505348516 1106 94-95 0.15375103204235785 1106 96-97 0.265266502417564 1106 98-99 0.2593214822107939 1106 100-101 0.20333778059248075 1107 1 -0.05392379984255413 1107 2 -0.05680933391208853 1107 3 0.015321353766140078 1107 4 0.019468825791541633 1107 5 -0.043028828486448845 1107 6 -0.026209662805833034 1107 7 -0.05365919420318477 1107 8 0.05186981712859762 1107 9 0.04017768741969263 1107 10-11 0.016517765070304335 1107 12-13 -0.08325673471052397 1107 14-15 0.05272845349393407 1107 16-17 -0.022740664066709826 1107 18-19 -0.005165172252539207 1107 20-21 -0.06333734922108647 1107 22-23 0.029821904637010732 1107 24-25 0.0742851697606568 1107 26-27 0.0705296345542763 1107 28-29 -0.03536805548190358 1107 30-31 0.049086583282061724 1107 32-33 0.036771936678938744 1107 34-35 0.05137366799831966 1107 36-37 0.13560583838305718 1107 38-39 0.17018170112692133 1107 40-41 0.1940589142185445 1107 42-43 0.13403087121908897 1107 44-45 0.29977233447414875 1107 46-47 0.1584552866863831 1107 48-49 0.1475488516919654 1107 50-51 0.05140235920446656 1107 52-53 0.07106478495115454 1107 54-55 0.10172730661328444 1107 56-57 0.15521623906031579 1107 58-59 0.2065469580218391 1107 60-61 0.007287470979498778 1107 62-63 0.054828115071266836 1107 64-65 0.016982669023100527 1107 66-67 0.09900984982158434 1107 68-69 0.02623319667731039 1107 70-71 0.014867525921708591 1107 72-73 0.14141207688728485 1107 74-75 0.15477555242727092 1107 76-77 0.13930785435510273 1107 78-79 0.08107923950732143 1107 80-81 0.3526911003396336 1107 82-83 0.3036849942450637 1107 84-85 0.20159167560490587 1107 86-87 0.29632327014862625 1107 88-89 0.11885618706476109 1107 90-91 0.07524240184626407 1107 92-93 0.05708587836623025 1107 94-95 -0.0013453513929881922 1107 96-97 0.08775008427359055 1107 98-99 0.12960872567100168 1107 100-101 0.3121675553430805 1108 1 0.04074650121663481 1108 2 -6.689753794617559E-4 1108 3 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-0.5224955393282009 2316 82-83 -0.7112529642906402 2316 84-85 -0.6741762217428864 2316 86-87 -0.8365860036991641 2316 88-89 -0.6512253758467672 2316 90-91 -0.7164341966467731 2316 92-93 -0.6936118078525659 2316 94-95 -0.923570595774919 2316 96-97 -1.0197613461768746 2316 98-99 -0.8822136877667432 2316 100-101 -1.3084383489998608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 22.0 5 57.0 6 151.0 7 407.0 8 1158.0 9 2189.0 10 3863.0 11 6318.0 12 9536.0 13 13501.0 14 17497.0 15 23236.0 16 28763.0 17 36844.0 18 34764.0 19 27314.0 20 25664.0 21 26214.0 22 27412.0 23 30001.0 24 35046.0 25 42242.0 26 53181.0 27 66942.0 28 83448.0 29 105023.0 30 130133.0 31 161785.0 32 203049.0 33 257927.0 34 340970.0 35 464344.0 36 684740.0 37 931673.0 38 778084.0 39 146096.0 40 72.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.32776084406994 19.20647269267634 14.130654569453704 19.33511189380001 2 14.315815792352288 25.225425697602343 35.4766302564343 24.982128253611073 3 28.512022723994672 23.90023402646408 28.837404548967555 18.750338700573685 4 11.82028442354302 15.823494642239169 43.233507782717695 29.12271315150011 5 11.34506454918908 40.85726256767909 35.070743361058135 12.726929522073691 6 26.941522891246965 39.866632581011245 16.23291836756379 16.958926160177995 7 25.91941159324812 34.69688455859389 21.34168306882573 18.04202077933226 8 23.360216414743252 27.817540903307368 19.642796498844817 29.179446183104556 9 20.786457744100485 23.631651572930075 25.553324153541297 30.02856652942815 10-11 25.817144209615943 23.96284080186296 27.183403952727613 23.03661103579348 12-13 32.10715232207534 22.71479176077032 24.55410494717139 20.62395096998295 14-15 23.629319496083532 25.917591135103823 30.798711560638843 19.6543778081738 16-17 19.683528440375 28.969608971277832 29.842147579637196 21.50471500870997 18-19 18.659000311062858 34.15737905478144 27.961342366448715 19.222278267706987 20-21 22.52582761351403 26.63641910990752 25.4878051448629 25.349948131715543 22-23 19.853729603426068 27.831263487248346 27.249421705335102 25.06558520399048 24-25 25.36671769097603 27.084230529871515 26.714538677947434 20.83451310120502 26-27 23.81747516133969 23.316123280223135 32.89814912145177 19.968252436985402 28-29 19.171063864545676 32.70412927147063 28.895627681894798 19.229179182088895 30-31 21.40716304508179 27.056754184249503 26.782728661598586 24.753354109070116 32-33 22.0657713766246 27.640942756957987 28.622010650052417 21.671275216365 34-35 26.000532981401697 25.281445642251967 28.425744210182195 20.29227716616414 36-37 24.064988688631452 24.079815583848333 31.90141437952517 19.953781347995044 38-39 21.260338330192926 27.88638466925681 31.857022560904436 18.996254439645828 40-41 21.108364222804244 29.679426674751063 28.328895250835007 20.883313851609692 42-43 19.24916066084447 30.613445575139416 25.178413942015926 24.958979822000195 44-45 22.518649619891875 24.94862453355765 26.3090208390873 26.22370500746318 46-47 25.779667061050617 28.77649889774253 26.46640977016518 18.97742427104168 48-49 24.54732220699768 27.233287708321896 27.91295418702282 20.3064358976576 50-51 18.03465742813056 28.261361453419305 33.13910558491676 20.564875533533378 52-53 21.693878913790837 32.28220036621447 25.042302594576498 20.981618125418194 54-55 19.367546686440427 27.212364627501263 27.82159947012549 25.598489215932823 56-57 23.967232315440587 24.903194389791004 27.548959150204738 23.58061414456367 58-59 28.82758906650481 25.12993021700841 25.057794802457938 20.984685914028837 60-61 22.97277629720012 27.054055374611497 31.958151551491547 18.015016776696836 62-63 20.62404023861465 27.812514975923925 30.01301212450801 21.550432660953415 64-65 19.389372355749234 33.447388934952656 26.90434528074795 20.258893428550163 66-67 22.328582996677046 26.773092047450643 24.850782825511956 26.047542130360355 68-69 20.012176357756104 28.607834693283635 26.387821891749198 24.992167057211066 70-71 25.29907801361543 27.431837676718917 25.57707108327175 21.6920132263939 72-73 23.875172668120932 23.976492133834203 31.30910041732036 20.839234780724507 74-75 19.802394521533746 32.858702936423995 27.430875637245062 19.9080269047972 76-77 21.67881406500799 27.128673388259493 25.837820460466332 25.354692086266184 78-79 22.149330447016272 27.789180790504318 27.829985785444066 22.23150297703534 80-81 25.595347912239045 26.359611734310295 27.060745972910745 20.984294380539918 82-83 23.455567555269376 24.978136433447304 30.74162780360199 20.82466820768133 84-85 21.01314022486841 28.38343575570018 30.05635930025336 20.54706471917805 86-87 21.516465213458865 29.923807329156134 26.75712041750642 21.802607039878588 88-89 19.901393200239433 30.185894068945167 24.53721287863809 25.375499852177306 90-91 22.617419146153377 24.423359496794806 26.251332784332966 26.707888572718847 92-93 25.0496025855224 28.494104464722312 26.614789956515573 19.841502993239715 94-95 23.37779501068995 27.100312390518255 28.08244601140324 21.43944658738856 96-97 17.336599499407335 27.926688781419607 33.02229122925232 21.714420489920744 98-99 21.139461522754644 31.671406783263116 25.35615465073154 21.832977043250697 100-101 18.8114374439773 27.461404502318416 27.457372743308845 26.269785310395438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 253.0 1 191.0 2 194.0 3 278.0 4 262.5 5 262.0 6 413.5 7 777.5 8 1255.5 9 1633.5 10 1822.5 11 1946.0 12 2147.0 13 2477.5 14 2835.5 15 3176.0 16 3600.5 17 4102.0 18 4610.5 19 5204.5 20 5849.0 21 6561.5 22 7715.5 23 9365.0 24 11099.5 25 13418.5 26 16839.0 27 21129.5 28 27794.5 29 36165.5 30 44463.5 31 53665.5 32 64952.0 33 74296.5 34 82181.5 35 91651.5 36 99923.5 37 109295.5 38 116537.5 39 118173.0 40 118768.0 41 118987.0 42 120987.0 43 127263.0 44 130308.0 45 136741.5 46 163055.5 47 354058.0 48 743215.5 49 708336.0 50 328907.0 51 161830.5 52 107623.5 53 85423.0 54 73123.5 55 62633.5 56 51185.0 57 39597.0 58 30268.0 59 24494.0 60 19346.5 61 14335.5 62 10414.0 63 7334.5 64 4870.0 65 3150.0 66 2033.0 67 1246.0 68 755.0 69 438.5 70 239.0 71 127.5 72 62.5 73 32.5 74 22.5 75 15.0 76 13.0 77 11.0 78 9.0 79 9.0 80 5.5 81 2.0 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052816098096682834 2 0.0041252810347704935 3 0.04010689894915758 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.083475270086108E-5 14-15 0.0014167631836585533 16-17 0.0 18-19 0.0 20-21 0.010365289468678388 22-23 1.041737635043054E-5 24-25 0.0 26-27 0.005198270798864839 28-29 0.012375843104311482 30-31 0.010104855059917624 32-33 0.00541703570222388 34-35 0.005333696691420436 36-37 0.020022197345527497 38-39 0.04152366213281613 40-41 0.006312930068360907 42-43 0.010636141253789581 44-45 0.009302717080934473 46-47 0.0042607069273260904 48-49 8.229727316840127E-4 50-51 0.0013959284309576923 52-53 5.521209465728186E-4 54-55 9.583986242396096E-4 56-57 0.010281950457874943 58-59 0.007625519488515155 60-61 0.006406686455514781 62-63 0.006312930068360907 64-65 0.06629618309413995 66-67 0.02878321085623958 68-69 0.02144937790553648 70-71 0.013177981083294633 72-73 6.250425810258325E-5 74-75 1.7709539795731917E-4 76-77 3.5419079591463834E-4 78-79 3.2293866686334676E-4 80-81 4.4794718306851323E-4 82-83 4.4794718306851323E-4 84-85 4.4794718306851323E-4 86-87 0.00270851785111194 88-89 8.333901080344432E-5 90-91 8.438074843848737E-4 92-93 5.00034064820666E-4 94-95 0.0014896848181115672 96-97 0.003375229937539495 98-99 0.00443780232528341 100-101 0.005812896003540241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4799673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.172357768302344 #Duplication Level Percentage of deduplicated Percentage of total 1 73.09289684526354 22.78477930782173 2 15.519567248410587 9.67563005353365 3 4.817226769394338 4.504929489198108 4 2.0985508729016247 2.616671144202905 5 1.133195170544574 1.7662182638763926 6 0.6327444647690289 1.183448209901589 7 0.43809305612592514 0.9559475436956409 8 0.3141594063720897 0.7834471529366612 9 0.21823662122775053 0.6122655031551233 >10 1.3914221155603859 8.551215007387821 >50 0.18449855641963184 4.047151421767766 >100 0.14055295598359016 8.490891062588545 >500 0.010029743100059335 2.136363351372804 >1k 0.006218440721240417 3.8608406171888494 >5k 8.023794479019892E-4 1.792906091363489 >10k+ 0.0018053537577794757 26.237295780008917 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 261509 5.44847534404948 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 100503 2.093955150694641 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 97486 2.031096701796143 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 96292 2.006220007071315 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 76882 1.6018174571476016 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 62283 1.2976509024677305 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 60571 1.2619818058438566 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 57110 1.1898727267461762 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 52125 1.0860114845323838 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 46060 0.9596487094016612 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 45224 0.9422308561437415 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 43720 0.9108953880816464 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 25333 0.5278067901709137 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 23937 0.49872147540051165 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 23348 0.4864498060597045 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 21551 0.44900975545625715 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 21296 0.4436968935175376 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 20988 0.4372797896856724 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 20472 0.42652905729202806 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 15966 0.33264766162194803 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13663 0.28466522615186496 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 13008 0.2710184631328009 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12929 0.2693725176694329 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 12438 0.2591426540933101 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 11939 0.24874611249558043 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 11462 0.23880793545726972 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10688 0.2226818368668032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9355 0.1949091115165554 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 8156 0.16992824302822296 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7997 0.16661551734878605 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 7898 0.1645528768314008 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7819 0.16290693136803278 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 7676 0.15992756173180964 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 7410 0.1543855175133806 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 6995 0.14573909514252326 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 6405 0.1334465910490152 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5453 0.11361190647779545 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5445 0.11344522845618858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.11269517735895758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.083475270086108E-5 0.0 5 0.0 0.0 0.0 2.083475270086108E-5 0.0 6 0.0 0.0 0.0 2.083475270086108E-5 0.0 7 0.0 0.0 0.0 2.083475270086108E-5 0.0 8 0.0 0.0 0.0 2.083475270086108E-5 0.0 9 0.0 0.0 0.0 2.083475270086108E-5 0.0 10-11 0.0 0.0 0.0 2.1876490335904136E-4 0.0 12-13 0.0 0.0 0.0 2.9168653781205513E-4 0.0 14-15 0.0 0.0 0.0 4.166950540172216E-4 0.0 16-17 0.0 0.0 0.0 5.417035702223881E-4 0.0 18-19 0.0 1.041737635043054E-5 0.0 7.500510972309989E-4 0.0 20-21 0.0 2.083475270086108E-5 0.0 0.0011459113985473595 0.0 22-23 0.0 2.083475270086108E-5 0.0 0.0016042759579663031 0.0 24-25 0.0 2.083475270086108E-5 0.0 0.002291822797094719 0.0 26-27 0.0 2.083475270086108E-5 0.0 0.003052291270676148 0.0 28-29 0.0 2.083475270086108E-5 0.0 0.004864914755651062 0.0 30-31 0.0 2.083475270086108E-5 0.0 0.008042214542532377 0.0 32-33 0.0 2.083475270086108E-5 0.0 0.012052904437448133 0.0 34-35 0.0 2.083475270086108E-5 0.0 0.016261524483022073 0.0 36-37 0.0 3.125212905129162E-5 0.0 0.019959693087424915 0.0 38-39 0.0 4.166950540172216E-5 0.0 0.02610594513417893 0.0 40-41 0.0 6.250425810258323E-5 0.0 0.03389814264430098 0.0 42-43 0.0 7.292163445301377E-5 0.0 0.04045067236872178 0.0 44-45 0.0 8.333901080344432E-5 0.0 0.043555050521150086 0.0 46-47 0.0 8.333901080344432E-5 0.0 0.04790951383563005 0.0 48-49 0.0 8.333901080344432E-5 0.0 0.05463913895800818 0.0 50-51 0.0 8.333901080344432E-5 0.0 0.06217090205936946 0.0 52-53 0.0 8.333901080344432E-5 0.0 0.07882828684370789 0.0 54-55 0.0 8.333901080344432E-5 0.0 0.08846435996785615 0.0 56-57 0.0 8.333901080344432E-5 0.0 0.1167267020065742 0.0 58-59 0.0 8.333901080344432E-5 0.0 0.17717873696812264 0.0 60-61 0.0 8.333901080344432E-5 0.0 0.2233902184586325 0.0 62-63 0.0 9.375638715387486E-5 0.0 0.24469375309526295 0.0 64-65 0.0 1.041737635043054E-4 0.0 0.2903531136392 0.0 66-67 0.0 1.041737635043054E-4 0.0 0.460573043205235 0.0 68-69 0.0 1.041737635043054E-4 0.0 0.6453877170382232 0.0 70-71 0.0 1.041737635043054E-4 0.0 0.7420401348175178 0.0 72-73 0.0 1.041737635043054E-4 0.0 0.838661300467761 0.0 74-75 0.0 1.041737635043054E-4 0.0 0.9671804725030226 0.0 76-77 0.0 1.2500851620516647E-4 0.0 1.0123502163584894 0.0 78-79 0.0 1.2500851620516647E-4 0.0 1.1013458625202175 0.0 80-81 0.0 1.2500851620516647E-4 0.0 1.236980102602823 0.0 82-83 0.0 1.2500851620516647E-4 0.0 1.33175739263904 0.0 84-85 0.0 1.2500851620516647E-4 0.0 1.5486159161259527 0.0 86-87 0.0 1.2500851620516647E-4 0.0 2.0493062756566958 0.0 88-89 0.0 1.2500851620516647E-4 0.0 2.7343216923319567 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 3385 0.0 31.310574 1 GTACGCA 3250 0.0 30.988005 3 AGTACGC 3295 0.0 30.710896 2 TACGCAG 3280 0.0 30.692743 4 TACCTGG 2555 0.0 29.192886 2 CGCAGTG 3660 0.0 27.506063 6 ACCTGGG 2760 0.0 26.850761 3 CTAACGC 325 0.0 24.848873 3 TAACGCC 365 0.0 24.719202 4 ACGCAGT 4080 0.0 24.441778 5 ACGTGCG 400 0.0 23.744432 9 GTACCTG 3300 0.0 23.619719 1 TCTAACG 400 0.0 22.56638 2 GTACTAG 980 0.0 22.308817 1 CGAGACA 2900 0.0 22.107115 94-95 TATAACG 645 0.0 22.096823 2 ATAACGC 590 0.0 21.739664 3 CGCCTAC 405 0.0 19.932766 7 ACGAGAC 3425 0.0 19.897 94-95 GTATAAC 900 0.0 19.539076 1 >>END_MODULE