##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003777_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 213683 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.637940313454976 31.0 31.0 34.0 30.0 34.0 2 31.773599210044786 31.0 31.0 34.0 30.0 34.0 3 31.858013973970788 31.0 31.0 34.0 30.0 34.0 4 35.63525877117038 37.0 35.0 37.0 33.0 37.0 5 35.498696667493434 37.0 35.0 37.0 33.0 37.0 6 35.568304450985806 37.0 35.0 37.0 33.0 37.0 7 35.49496216357876 37.0 35.0 37.0 33.0 37.0 8 35.49894937828466 37.0 35.0 37.0 33.0 37.0 9 37.07582259702456 39.0 37.0 39.0 33.0 39.0 10-11 36.979860353888704 39.0 37.0 39.0 33.0 39.0 12-13 36.94101542939775 39.0 37.0 39.0 33.0 39.0 14-15 38.02262697547302 40.0 37.0 41.0 33.0 41.0 16-17 38.03576793661639 40.0 37.0 41.0 33.0 41.0 18-19 38.07406298114496 40.0 37.0 41.0 33.0 41.0 20-21 38.00708760172779 40.0 37.0 41.0 33.0 41.0 22-23 37.90093502992751 40.0 37.0 41.0 33.0 41.0 24-25 37.72638207063734 40.0 37.0 41.0 32.0 41.0 26-27 37.515310062101335 39.0 36.5 41.0 32.0 41.0 28-29 37.32217349999766 39.0 36.0 41.0 31.5 41.0 30-31 37.09864378542046 39.0 36.0 40.5 31.0 41.0 32-33 36.774371381906846 39.0 35.0 40.0 30.5 41.0 34-35 36.663166934196916 39.0 35.0 40.0 30.0 41.0 36-37 36.43302228066809 39.0 35.0 40.0 30.0 41.0 38-39 36.15035824094571 38.0 35.0 40.0 30.0 41.0 40-41 36.186645638632925 39.0 35.0 40.0 30.0 41.0 42-43 36.021473397509396 39.0 35.0 40.0 29.5 41.0 44-45 35.601716093465555 39.0 35.0 40.0 27.5 41.0 46-47 35.48051319009936 39.0 35.0 40.0 27.0 41.0 48-49 35.21671588287323 38.5 35.0 40.0 26.0 41.0 50-51 34.921381204868894 38.0 34.0 40.0 25.0 41.0 52-53 34.67425813003374 38.0 34.0 40.0 24.0 41.0 54-55 34.481147306992135 38.0 34.0 40.0 23.5 41.0 56-57 34.15211551691057 38.0 33.5 40.0 23.0 41.0 58-59 33.868496791976895 38.0 33.0 40.0 22.0 41.0 60-61 33.45067927724713 37.0 33.0 40.0 20.5 41.0 62-63 33.144419537352064 37.0 32.0 40.0 19.5 41.0 64-65 32.6224524178339 36.0 31.5 39.0 16.0 41.0 66-67 32.25784222422935 36.0 31.0 39.0 13.0 40.5 68-69 31.935640645254885 35.5 31.0 39.0 12.0 40.0 70-71 31.504539434583002 35.0 31.0 38.0 8.0 40.0 72-73 31.086794457209976 35.0 30.5 37.5 7.0 40.0 74-75 30.705678505075277 35.0 30.0 37.0 6.5 39.0 76-77 29.71497030648203 34.0 29.0 36.0 2.0 39.0 78-79 30.1113074039582 34.0 30.0 36.0 2.0 39.0 80-81 29.888821291352144 34.0 30.0 36.0 2.0 37.5 82-83 29.60809236111436 34.0 29.5 35.0 2.0 37.0 84-85 29.272436272422233 34.0 29.0 35.0 2.0 37.0 86-87 28.90713346405657 34.0 29.0 35.0 2.0 36.0 88-89 28.564087924636027 34.0 29.0 35.0 2.0 36.0 90-91 28.252827786955443 34.0 29.0 35.0 2.0 36.0 92-93 27.98812259281272 34.0 28.5 35.0 2.0 35.0 94-95 27.624888269071477 34.0 27.0 35.0 2.0 35.0 96-97 27.249374072808784 34.0 27.0 35.0 2.0 35.0 98-99 26.87003645587155 34.0 26.0 35.0 2.0 35.0 100-101 25.956114899173073 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.05644594186524898 1101 2 0.15809808004070547 1101 3 0.06092069484394358 1101 4 0.057632421972421355 1101 5 -0.02393322506826223 1101 6 -0.05038362442415689 1101 7 -0.05671039083738805 1101 8 -0.20548900757567878 1101 9 -0.10925931435357938 1101 10-11 0.17211562796993718 1101 12-13 0.1884021876364983 1101 14-15 0.17598102807952642 1101 16-17 0.11333482009373341 1101 18-19 0.25980547450888736 1101 20-21 0.019999550040850522 1101 22-23 -0.06252389883709242 1101 24-25 0.1513961050118766 1101 26-27 0.3099418539863734 1101 28-29 0.3903028318281514 1101 30-31 0.16616652703950052 1101 32-33 0.18060924020921476 1101 34-35 0.16524273452486682 1101 36-37 0.33083608235747874 1101 38-39 0.41068205098461874 1101 40-41 0.49316560796889775 1101 42-43 0.693839517504081 1101 44-45 0.639348800508408 1101 46-47 0.5256043194853461 1101 48-49 0.5510500598749033 1101 50-51 0.39553343808744756 1101 52-53 0.3344816902382277 1101 54-55 0.39684225147964725 1101 56-57 0.24977126305950037 1101 58-59 0.290962785838353 1101 60-61 0.48912149212451084 1101 62-63 0.6467888871113843 1101 64-65 0.7009265420944715 1101 66-67 0.7943262213644431 1101 68-69 1.0108382518930341 1101 70-71 1.269931869377885 1101 72-73 1.0767951135370986 1101 74-75 0.7838689973426476 1101 76-77 0.4065130321957433 1101 78-79 0.7709563633746193 1101 80-81 0.9898806725090203 1101 82-83 1.0154398897247852 1101 84-85 0.7623765793154966 1101 86-87 0.8919244895221077 1101 88-89 0.7134773156565366 1101 90-91 0.7972907666965625 1101 92-93 0.6232233830135705 1101 94-95 0.8105196548269831 1101 96-97 1.12727078325209 1101 98-99 0.9173002585136096 1101 100-101 0.6247052095894219 1102 1 0.17348370387487222 1102 2 0.23495763472629605 1102 3 0.19573840954910793 1102 4 0.12344067578532503 1102 5 0.2832399255719764 1102 6 0.13942552654232543 1102 7 0.15595578647278074 1102 8 0.23592074693047493 1102 9 -0.1577973618211601 1102 10-11 0.38166215761678046 1102 12-13 0.20399023102611125 1102 14-15 0.08488425783239961 1102 16-17 0.33173564384383525 1102 18-19 0.2909356841165831 1102 20-21 0.2787518948740626 1102 22-23 0.361488212736198 1102 24-25 0.4659911625112656 1102 26-27 0.1628443497044998 1102 28-29 -0.008436055701416478 1102 30-31 -0.2123405278499817 1102 32-33 0.0974849028794651 1102 34-35 0.3035726015830136 1102 36-37 0.7302886147773435 1102 38-39 0.2368279607799053 1102 40-41 0.3325394355522988 1102 42-43 0.3677853620518263 1102 44-45 0.9516754867493304 1102 46-47 1.0079925830853824 1102 48-49 0.8051942977021653 1102 50-51 0.76731693861759 1102 52-53 0.6749004978395732 1102 54-55 0.5491799482417434 1102 56-57 0.9642980145479569 1102 58-59 0.9028470122472925 1102 60-61 0.7673002703644585 1102 62-63 0.3259392418948437 1102 64-65 0.31144464828483365 1102 66-67 0.7034486420479098 1102 68-69 0.7945222907153209 1102 70-71 0.8851192521362208 1102 72-73 1.2440225011890007 1102 74-75 0.9142060416294093 1102 76-77 0.8353065645340543 1102 78-79 0.8439418457985646 1102 80-81 1.0644106863741207 1102 82-83 0.8562002273807678 1102 84-85 1.0992220650018218 1102 86-87 0.8094628224476459 1102 88-89 0.9702875268954223 1102 90-91 1.0189131873800292 1102 92-93 0.8793607290701715 1102 94-95 0.9791896246500009 1102 96-97 0.8135320996210673 1102 98-99 0.2758864260366529 1102 100-101 0.6492189115713138 1103 1 -0.3545535293140212 1103 2 -0.1257067851100082 1103 3 -0.16694815761507087 1103 4 -0.10137075095195769 1103 5 0.015754770701171594 1103 6 0.06942071190582766 1103 7 -0.1957373606946078 1103 8 -0.15674495680889322 1103 9 0.09429700505413763 1103 10-11 0.13293001189378373 1103 12-13 0.2237141918670673 1103 14-15 -0.16619903537895908 1103 16-17 -0.04653429677565413 1103 18-19 -0.06020377985388592 1103 20-21 -0.2893671871352481 1103 22-23 -0.1292676851935184 1103 24-25 -0.00955048409441872 1103 26-27 -0.075456083612778 1103 28-29 -0.24241134056740776 1103 30-31 -0.11179486904722324 1103 32-33 0.06894213285860218 1103 34-35 0.27710814964913766 1103 36-37 0.30852645782019295 1103 38-39 0.4302880795409365 1103 40-41 0.2222441378472766 1103 42-43 0.836145175885882 1103 44-45 0.8660872986575328 1103 46-47 1.0011397504742447 1103 48-49 0.9651365220642205 1103 50-51 1.013625717304798 1103 52-53 1.1455472640087265 1103 54-55 0.8992682165774895 1103 56-57 0.9247778733186252 1103 58-59 1.100977328408419 1103 60-61 1.2518131896390514 1103 62-63 1.205164085419156 1103 64-65 0.9719405558438154 1103 66-67 1.2785409225807314 1103 68-69 0.4204892302113876 1103 70-71 0.9442510126882979 1103 72-73 0.9137464620299589 1103 74-75 0.6794202929534379 1103 76-77 0.6472388386473575 1103 78-79 0.9204143221266001 1103 80-81 0.6347445011711024 1103 82-83 0.7031910795714253 1103 84-85 0.7714656856084652 1103 86-87 1.526859708982709 1103 88-89 1.2000981511932878 1103 90-91 0.6020699840418828 1103 92-93 0.4558251810040481 1103 94-95 0.8832258949115968 1103 96-97 0.4015436547486537 1103 98-99 0.1772024266786012 1103 100-101 -0.07971705376330362 1104 1 -0.06210663710316311 1104 2 -0.03963282759623965 1104 3 0.04280793994590226 1104 4 0.053703993343873435 1104 5 0.0647712931244584 1104 6 0.2055340085226689 1104 7 -0.02028496100931676 1104 8 0.06481287167262195 1104 9 -0.1296211696114753 1104 10-11 0.06577816295594374 1104 12-13 -0.026521842751350277 1104 14-15 -0.05412193073694738 1104 16-17 0.10724225711891933 1104 18-19 0.14392682596830042 1104 20-21 0.37432282073257994 1104 22-23 0.08198329828406514 1104 24-25 0.28246367798268324 1104 26-27 0.15094045454614502 1104 28-29 0.02635341159624005 1104 30-31 -0.2424400303375407 1104 32-33 0.0069437064227244605 1104 34-35 -0.014040552160459185 1104 36-37 -0.3959581999296091 1104 38-39 -0.24020759315772722 1104 40-41 -0.0957338322550143 1104 42-43 -0.3466242969701341 1104 44-45 -0.32938870448959534 1104 46-47 -0.497601398227566 1104 48-49 -0.6441868453630022 1104 50-51 -0.41658671385177115 1104 52-53 -0.7507941994059095 1104 54-55 -0.5603698636742891 1104 56-57 -0.23183109599687413 1104 58-59 -0.3079526479881167 1104 60-61 -0.31057862782751045 1104 62-63 -0.23059515927006657 1104 64-65 -0.3172411908123607 1104 66-67 -0.9368013276159672 1104 68-69 -0.7054352387107201 1104 70-71 -0.6847812453762181 1104 72-73 -0.4242944506372339 1104 74-75 -0.2556701869836182 1104 76-77 -0.2072064799993818 1104 78-79 -0.3510648281487754 1104 80-81 -0.28252536507594783 1104 82-83 -0.7235595105151198 1104 84-85 -0.7170866353986156 1104 86-87 -0.4430065380668573 1104 88-89 -0.8672503932598978 1104 90-91 -0.45182458716189444 1104 92-93 -0.27375782450342356 1104 94-95 -0.18199833797589093 1104 96-97 0.45170101116092454 1104 98-99 0.18916151401141335 1104 100-101 0.5965614670864241 1105 1 -0.27366941939765255 1105 2 -0.5276183741183686 1105 3 -0.44842963909655253 1105 4 -0.44223096600572376 1105 5 -0.7836478577337189 1105 6 0.008966709516343485 1105 7 -0.17638252198492665 1105 8 -0.18613563781247677 1105 9 -0.26006380737118917 1105 10-11 -0.47035553171433975 1105 12-13 0.2657094128945445 1105 14-15 -0.24797920264300188 1105 16-17 -0.046235610993100806 1105 18-19 -0.16456459227921982 1105 20-21 -0.5010159326549584 1105 22-23 -0.32795346775026246 1105 24-25 -0.7235887158745911 1105 26-27 -0.6777858327167223 1105 28-29 -1.141216597437186 1105 30-31 -1.2905881784856916 1105 32-33 0.08734117706409705 1105 34-35 0.1328429166742282 1105 36-37 0.09717639808303602 1105 38-39 -0.2913367711161712 1105 40-41 -0.07387294697949187 1105 42-43 0.40567922606509654 1105 44-45 0.6244505005691181 1105 46-47 0.035189929685707 1105 48-49 0.1933921970940915 1105 50-51 0.10382882363693824 1105 52-53 0.1511028195642794 1105 54-55 0.3569743097674447 1105 56-57 0.5506440620880326 1105 58-59 0.5401051659233573 1105 60-61 0.3371178491372717 1105 62-63 0.45540303404519733 1105 64-65 0.8820361352942356 1105 66-67 0.9211932523298287 1105 68-69 1.1795692780011109 1105 70-71 1.337440976846004 1105 72-73 1.315000942316697 1105 74-75 1.680734510396686 1105 76-77 0.9662352544179633 1105 78-79 0.4123498059975681 1105 80-81 0.5515306421507908 1105 82-83 0.6527729194758471 1105 84-85 0.6967345028127667 1105 86-87 0.41670439237578094 1105 88-89 0.44322837819448324 1105 90-91 1.068139279143434 1105 92-93 1.0982385621271078 1105 94-95 0.7465472808136298 1105 96-97 0.7692258530760121 1105 98-99 0.8988272773357124 1105 100-101 1.2306174743131635 1106 1 -0.18461218034041593 1106 2 0.04856007706008114 1106 3 0.07396093329401765 1106 4 -0.09829157206632999 1106 5 0.2912679671821081 1106 6 -0.4838784083287706 1106 7 -0.3763825219849295 1106 8 -0.06744876912560471 1106 9 -0.37285842016579807 1106 10-11 0.11874379488498477 1106 12-13 -0.6892990046138721 1106 14-15 -0.022137451801249597 1106 16-17 -0.44627769853518373 1106 18-19 -0.23190465961928197 1106 20-21 0.051761845122818784 1106 22-23 -0.1676841074809019 1106 24-25 0.2817984895126173 1106 26-27 0.2800424501115586 1106 28-29 -0.2595667657873477 1106 30-31 -0.4026673030648098 1106 32-33 -0.37797868825576586 1106 34-35 0.40658029041159693 1106 36-37 -0.46145154804490573 1106 38-39 -0.6146532694326723 1106 40-41 -0.5255564486629893 1106 42-43 0.049744882630754717 1106 44-45 -0.47559158697296766 1106 46-47 -0.7786989592031759 1106 48-49 -1.1119529207510297 1106 50-51 0.34225474956286206 1106 52-53 0.5171381730996316 1106 54-55 -0.6447933670002257 1106 56-57 -0.3202734463294732 1106 58-59 -0.23586284754465225 1106 60-61 -0.4796750801556655 1106 62-63 -0.46349427235210783 1106 64-65 -0.8518864236283257 1106 66-67 -1.507089575952996 1106 68-69 -0.26580109236925864 1106 70-71 -0.009781245376217385 1106 72-73 0.35073326554902096 1106 74-75 0.37850387066604796 1106 76-77 -0.4173506041678934 1106 78-79 0.14530435145211484 1106 80-81 -0.3012892231690749 1106 82-83 -0.49284997614704906 1106 84-85 0.030446624024886404 1106 86-87 -0.22618281301142673 1106 88-89 -0.26603088106477557 1106 90-91 0.2004204239245766 1106 92-93 0.13998940387795145 1106 94-95 0.14444290370925117 1106 96-97 0.13332517967533875 1106 98-99 0.3255528665612992 1106 100-101 0.3425703362660215 1107 1 -0.10523141617704113 1107 2 -0.42294848683978614 1107 3 -0.20173076631072462 1107 4 0.047624106458044935 1107 5 -0.3697992264937824 1107 6 -0.47251477196513747 1107 7 -0.11551295676753881 1107 8 -0.6547346716289013 1107 9 -0.949274757451704 1107 10-11 -0.5320302499107967 1107 12-13 -0.6160941297785598 1107 14-15 -0.7502336309843827 1107 16-17 -1.0059779620397933 1107 18-19 -1.3682682959829222 1107 20-21 -1.0768613432829781 1107 22-23 -0.3363270587325502 1107 24-25 -0.6707706804478519 1107 26-27 -0.3657085380307308 1107 28-29 -0.02076571176890951 1107 30-31 -0.1453385810621839 1107 32-33 -0.5762659082821173 1107 34-35 -0.9260942813934037 1107 36-37 -0.6601504940264675 1107 38-39 -0.29004852634967193 1107 40-41 -0.17805974247063006 1107 42-43 -0.6107458947078541 1107 44-45 -0.9628280823616322 1107 46-47 -0.11022069833361314 1107 48-49 -0.45475595105405375 1107 50-51 0.28337793796865896 1107 52-53 0.32453277125510027 1107 54-55 -0.5652479124547725 1107 56-57 -0.6833333936285513 1107 58-59 0.17907457011015282 1107 60-61 -0.43776137791587644 1107 62-63 -0.15297055693709183 1107 64-65 -0.6949364895044781 1107 66-67 0.417811609817754 1107 68-69 0.5367351395147999 1107 70-71 -0.5152160279849127 1107 72-73 -0.28258359874610406 1107 74-75 -0.27111734145516664 1107 76-77 0.6338681046595127 1107 78-79 0.557277342229451 1107 80-81 0.40531486115240156 1107 82-83 1.0601803268832555 1107 84-85 1.0025316042620425 1107 86-87 1.1856254873838346 1107 88-89 1.2665778145873965 1107 90-91 0.12820369138175636 1107 92-93 0.3916363076987679 1107 94-95 0.507255815435208 1107 96-97 0.5278409899520184 1107 98-99 1.6917419311199318 1107 100-101 0.9423727078075217 1108 1 -0.2826641283923621 1108 2 0.1300535835535861 1108 3 -0.1383767290436424 1108 4 0.11014998637522666 1108 5 0.17243679835094383 1108 6 0.22748522803486537 1108 7 0.06647462087221356 1108 8 -0.3655007171775537 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0.3777138244209013 2316 50-51 -0.058328151991538846 2316 52-53 0.3991742070139068 2316 54-55 0.24304224298564492 2316 56-57 0.10374327529087424 2316 58-59 0.5838203837318403 2316 60-61 0.5700481888033551 2316 62-63 0.5367118039550007 2316 64-65 0.7661658608747288 2316 66-67 0.820041841852003 2316 68-69 1.275002016439032 2316 70-71 0.861332744261091 2316 72-73 0.33733830558670164 2316 74-75 0.7219741956778201 2316 76-77 0.722251374164653 2316 78-79 1.0703808940804684 2316 80-81 1.0941712101799617 2316 82-83 0.7199385306829065 2316 84-85 0.9680643819146653 2316 86-87 0.8587500649914013 2316 88-89 0.5124665282616512 2316 90-91 0.7352473198266054 2316 92-93 0.5634467755218502 2316 94-95 0.08197234318169677 2316 96-97 -0.34656766064496125 2316 98-99 -0.2600865587801984 2316 100-101 -0.8904725276058585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 9.0 6 17.0 7 49.0 8 121.0 9 223.0 10 368.0 11 634.0 12 958.0 13 1330.0 14 1401.0 15 1632.0 16 1974.0 17 2411.0 18 2311.0 19 1753.0 20 1661.0 21 1574.0 22 1713.0 23 1720.0 24 2009.0 25 2245.0 26 2687.0 27 3299.0 28 3959.0 29 4904.0 30 5921.0 31 7185.0 32 8757.0 33 11130.0 34 14194.0 35 18728.0 36 27288.0 37 37776.0 38 34564.0 39 7171.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.42882228163909 19.696380825728028 13.64319596173387 20.23160093089901 2 15.934256217298925 23.542901000570954 38.44008274132105 22.08276004080907 3 21.263668364289995 27.210811114439785 30.20568079688436 21.31983972438586 4 12.506844250595508 18.115619866812054 39.495420786866525 29.882115095725915 5 11.812357557690598 39.16221692881511 33.611471197989545 15.413954315504744 6 26.79763949401684 39.19637968392431 18.599046250754622 15.40693457130422 7 27.409293205355596 32.56740124389867 22.540398627874 17.48290692287173 8 24.10626956753696 32.94365953304661 20.77282703818273 22.1772438612337 9 23.811908294061766 20.547727240819345 23.213358105230643 32.427006359888246 10-11 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30.957584720577707 20.43543171107253 36-37 22.538029487479523 26.957641001638194 31.26889772993213 19.23543178095015 38-39 21.456376357350347 28.131064040954 30.915870611945856 19.496688989749796 40-41 20.3956943956944 28.644202644202643 30.316602316602314 20.64350064350064 42-43 20.412275118407997 28.95286144860251 28.779227586723326 21.855635846266168 44-45 21.542180403095408 27.95763590371201 28.729849324781263 21.770334368411323 46-47 22.49894114646343 28.780692127511703 28.19381816735268 20.526548558672186 48-49 22.18363908648446 28.617090977162114 28.93976974915762 20.259500187195805 50-51 20.72702171471359 28.73830026207413 30.399663047547737 20.135014975664546 52-53 21.015155734118302 29.54303484391489 28.71774112405613 20.724068297910677 54-55 20.09041599782852 29.526298793985372 28.69186958007104 21.69141562811507 56-57 21.88073351555772 27.94492080727899 28.803309993634628 21.371035683528664 58-59 22.54558048867245 27.472789776303998 28.75613414739739 21.225495587626163 60-61 21.091007796924284 28.510955942229753 30.059342737066746 20.338693523779213 62-63 20.60312208226179 28.02034899366551 29.571327949679766 21.805200974392935 64-65 20.05405076275275 29.724875060303603 29.2286291059142 20.992445071029447 66-67 21.043477446645145 27.301158509037027 28.425256386547343 23.230107657770485 68-69 20.25592292820395 29.064362252421365 28.15011773297319 22.52959708640149 70-71 22.151803935518902 28.184737226414036 27.682967927954916 21.98049091011215 72-73 20.830856923573705 27.535882592438334 29.829233022748653 21.80402746123931 74-75 19.91829017750593 29.733062527201508 28.414754566343603 21.933892728948955 76-77 20.93498312921477 27.668555757828184 27.934136080081238 23.462325032875803 78-79 20.844428429028046 28.820729772607084 28.04177215782257 22.2930696405423 80-81 21.100513847680197 27.692318491964695 28.289701519079753 22.91746614127535 82-83 20.31383083273275 26.537799159498693 29.54647560393482 23.601894403833732 84-85 19.38081176321935 27.4111651371424 29.074610521192607 24.133412578445643 86-87 20.082464962921186 28.031301116459527 27.885045152141302 24.00118876847798 88-89 19.689446516568935 27.848027217888184 27.216952214261315 25.245574051281572 90-91 19.869993424793442 26.635904371693385 28.140948093307095 25.353154110206077 92-93 20.182794994430978 28.606527456687974 27.549115040106326 23.661562508774722 94-95 19.417995979885387 29.05784706248494 27.45377921193586 24.070377745693822 96-97 18.364777519318164 29.44926261004685 27.95973958503971 24.22622028559527 98-99 19.56536493010782 29.600913641671795 25.92832687028582 24.90539455793457 100-101 18.554560879995318 27.697852670996433 26.752091744192853 26.9954947048154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 54.0 2 93.0 3 130.5 4 91.5 5 79.0 6 98.0 7 137.0 8 184.0 9 214.0 10 227.5 11 227.5 12 223.0 13 257.5 14 292.5 15 318.5 16 360.5 17 383.0 18 397.5 19 443.5 20 483.5 21 518.0 22 578.0 23 693.0 24 839.5 25 1008.5 26 1243.0 27 1552.5 28 2014.0 29 2480.5 30 2963.0 31 3463.5 32 3955.0 33 4328.5 34 4691.5 35 5197.5 36 5621.0 37 5996.5 38 6387.0 39 6587.0 40 6751.0 41 7002.0 42 7152.5 43 7375.0 44 7492.5 45 7653.5 46 8313.5 47 10267.0 48 15342.0 49 15246.0 50 10354.5 51 8147.0 52 6471.5 53 5364.5 54 4661.0 55 4180.5 56 3733.5 57 3182.5 58 2604.5 59 2019.5 60 1537.0 61 1196.5 62 902.0 63 656.0 64 446.5 65 302.5 66 186.5 67 113.5 68 76.0 69 46.5 70 25.0 71 9.5 72 8.0 73 4.5 74 3.0 75 3.0 76 2.0 77 1.5 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0589658512843792 2 0.0023399147335071107 3 0.023867130281772533 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0018719317868056886 16-17 0.0 18-19 0.0 20-21 0.0084236930406256 22-23 0.0 24-25 0.0 26-27 0.004913820940364933 28-29 0.009827641880729866 30-31 0.008657684513976312 32-33 0.0042118465203128 34-35 0.0046798294670142215 36-37 0.015443437241146931 38-39 0.03626867836936022 40-41 0.003743863573611377 42-43 0.0070197442005213335 44-45 0.005381803887066355 46-47 0.0039778550469620885 48-49 0.0014039488401042667 50-51 0.0014039488401042667 52-53 7.019744200521333E-4 54-55 0.0018719317868056886 56-57 0.0126355395609384 58-59 0.010529616300782 60-61 0.0042118465203128 62-63 0.005381803887066355 64-65 0.084236930406256 66-67 0.041884473729777286 68-69 0.029950908588891018 70-71 0.018251334921355465 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.6798294670142215E-4 82-83 4.6798294670142215E-4 84-85 0.0 86-87 0.007721718620573466 88-89 0.0 90-91 7.019744200521333E-4 92-93 4.6798294670142215E-4 94-95 0.0035098721002606668 96-97 0.011231590720834133 98-99 0.015209445767796221 100-101 0.021293224074914713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 213683.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.61581169752655 #Duplication Level Percentage of deduplicated Percentage of total 1 87.0670974593288 58.0004536940084 2 8.623686031555277 11.489476896333528 3 1.851313660661972 3.6998028663514946 4 0.8164897240701593 2.1756450284649254 5 0.4408913368497077 1.4685167137325439 6 0.27584321989191046 1.102531199661535 7 0.1834050121845826 0.8552371629249502 8 0.12509268286281014 0.6666520485061893 9 0.0866403374634724 0.519445476528915 >10 0.4547372409089608 5.3975484359286385 >50 0.023622092912320727 1.0969337444051104 >100 0.04232291646790797 6.571111767141671 >500 0.007874030970773575 4.063834637072848 >1k 9.842538713466969E-4 2.892810328939239 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4412 2.064740760846675 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 951 0.4450517823130525 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 930 0.43522414043232266 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 827 0.38702189692207617 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 825 0.38608593102867333 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 824 0.3856179480819719 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 652 0.30512488124932724 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 640 0.2995090858889102 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 549 0.2569226377390808 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 492 0.23024760977709974 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 458 0.21433618958925138 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 438 0.20497653065522292 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 434 0.20310459886841722 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 427 0.19982871824150727 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 414 0.1937449399343888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.19093704225418026 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 363 0.16987780965261626 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 341 0.15958218482518496 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 341 0.15958218482518496 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.14460673053073947 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 306 0.1432027816906352 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 281 0.13150320802309964 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 270 0.126355395609384 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 257 0.1202716173022655 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 242 0.11325187310174417 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 232 0.10857204363472996 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 229 0.10716809479462569 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 219 0.10248826532761146 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.679829467014222E-4 0.0 12-13 0.0 0.0 0.0 4.679829467014222E-4 0.0 14-15 0.0 0.0 0.0 4.679829467014222E-4 0.0 16-17 0.0 0.0 0.0 4.679829467014222E-4 0.0 18-19 0.0 0.0 0.0 0.0011699573667535556 0.0 20-21 0.0 0.0 0.0 0.0018719317868056888 0.0 22-23 0.0 0.0 0.0 0.0037438635736113776 0.0 24-25 0.0 0.0 0.0 0.007487727147222755 0.0 26-27 0.0 0.0 0.0 0.012167556614236977 0.0 28-29 0.0 0.0 0.0 0.021995198494966846 0.0 30-31 0.0 0.0 0.0 0.045862328776739375 0.0 32-33 0.0 0.0 0.0 0.07768516915243609 0.0 34-35 0.0 0.0 0.0 0.10365822269436502 0.0 36-37 0.0 0.0 0.0 0.13173719949645035 0.0 38-39 0.0 0.0 0.0 0.19748880350800016 0.0 40-41 0.0 0.0 0.0 0.3374157045717254 0.0 42-43 0.0 0.0 0.0 0.5545597918411853 0.0 44-45 0.0 0.0 0.0 0.7478367488288726 0.0 46-47 0.0 0.0 0.0 1.1830608892611953 0.0 48-49 0.0 0.0 0.0 1.564700982296205 0.0 50-51 0.0 0.0 0.0 1.7944806091266035 0.0 52-53 0.0 0.0 0.0 2.1129430043569215 0.0 54-55 0.0 0.0 0.0 2.345764520340879 0.0 56-57 0.0 0.0 0.0 2.9447826921186993 0.0 58-59 0.0 0.0 0.0 3.677643986653126 0.0 60-61 0.0 0.0 0.0 4.285085851471573 0.0 62-63 0.0 0.0 0.0 4.91311896594488 0.0 64-65 0.0 0.0 0.0 5.779121408815863 0.0 66-67 0.0 0.0 0.0 6.778966974443451 0.0 68-69 0.0 0.0 0.0 7.743245836121732 0.0 70-71 0.0 0.0 0.0 8.4564518468947 0.0 72-73 0.0 0.0 0.0 8.967489224692653 0.0 74-75 0.0 0.0 0.0 9.683971116092529 0.0 76-77 0.0 0.0 0.0 10.485157920845364 0.0 78-79 0.0 0.0 0.0 11.167711048609387 0.0 80-81 0.0 0.0 0.0 12.244773800442712 0.0 82-83 0.0 0.0 0.0 13.393203951648001 0.0 84-85 0.0 0.0 0.0 15.307020212183469 0.0 86-87 0.0 0.0 0.0 16.655513073103617 0.0 88-89 0.0 0.0 0.0 17.84746563835214 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 40 7.5008065E-6 59.406624 3 TATAACG 40 7.5008065E-6 59.406624 2 TACCTGG 330 0.0 50.40562 2 ACCTGGG 345 0.0 46.83653 3 TAACGCA 55 4.961513E-5 43.16437 4 CCTGGGG 250 0.0 39.883877 4 GTACCTG 440 0.0 38.939064 1 GAGTAGG 135 7.2759576E-12 38.77882 1 GGCCTAA 50 0.001616077 37.98465 6 GACCATC 125 1.3460522E-10 37.984646 7 GTTGTAA 65 1.3095343E-4 36.609375 1 GATCTAT 55 0.0025505659 34.612503 1 CCTATAG 55 0.002568227 34.563854 2 GTCTTAT 60 0.003906543 31.728125 1 GTAGGCA 120 1.4891157E-7 31.68353 3 TATACTG 75 3.077412E-4 31.653873 5 CTGGGGG 140 1.6989361E-8 30.523376 5 AGTAGGC 160 1.875378E-9 29.703312 2 CGAATGG 40 4.4934385E-4 29.682453 50-51 ACGCAGT 80 4.492156E-4 29.675508 5 >>END_MODULE