FastQCFastQC Report
Wed 25 May 2016
SRR1003776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856552
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.2640917141022713No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42150.22703377012871173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35510.19126854513097397No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24190.13029530010471024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.12156944701791278No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21900.11796060654374345No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20490.11036588256079011No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20180.10869612055035356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4100.053.236274
TATAACG4450.051.708062
TACCTGG24600.051.4453162
ACCTGGG28100.045.5858543
GTACCTG30600.042.8934861
ATAACGC5650.039.650413
GTACACG2950.039.0288621
GTACCCG2800.032.8957561
TACACGG3600.031.9584562
GTATAAC8550.030.9720271
CCTGGGG20650.028.3718724
ACACGGG4100.028.0206153
TACCCGG3800.024.2211462
TCACGCA3600.023.9329724
GTATACG3203.092282E-1123.3868261
TATCACG3800.022.7073252
AGTACTT65100.022.24085612-13
GAGTACT62650.022.1476712-13
GTACATG73200.021.7058071
TACATGG72050.020.918262
Produced by FastQC (version 0.11.5)