##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003776_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856552 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.885059508163522 31.0 31.0 34.0 28.0 34.0 2 30.67890314949433 31.0 31.0 34.0 27.0 34.0 3 30.815587174504135 31.0 31.0 34.0 27.0 34.0 4 34.281641451464864 37.0 35.0 37.0 30.0 37.0 5 34.197104632673906 37.0 35.0 37.0 30.0 37.0 6 34.28751955237451 37.0 35.0 37.0 30.0 37.0 7 34.243467460108846 37.0 35.0 37.0 30.0 37.0 8 34.29639945447259 37.0 35.0 37.0 30.0 37.0 9 35.798800141337274 39.0 35.0 39.0 30.0 39.0 10-11 35.716308242376186 39.0 35.0 39.0 30.0 39.0 12-13 35.70929766578044 39.0 35.0 39.0 30.0 39.0 14-15 36.757303054264035 40.0 37.0 41.0 30.5 41.0 16-17 36.683229987632984 40.0 36.5 41.0 30.0 41.0 18-19 36.57776135545894 40.0 36.0 41.0 30.0 41.0 20-21 36.52448059628817 40.0 36.0 41.0 29.5 41.0 22-23 36.33023610434827 39.0 36.0 41.0 29.0 41.0 24-25 36.29486596658752 39.0 36.0 41.0 29.0 41.0 26-27 36.182062500807945 39.0 36.0 41.0 28.5 41.0 28-29 35.98773290486881 39.0 36.0 41.0 27.0 41.0 30-31 35.70951985185441 39.0 35.0 40.0 27.0 41.0 32-33 35.46150983112781 38.5 35.0 40.0 26.5 41.0 34-35 35.39054898543105 39.0 35.0 40.0 25.5 41.0 36-37 35.39441798559911 39.0 35.0 40.0 26.0 41.0 38-39 35.33516324886133 39.0 35.0 40.5 25.0 41.0 40-41 35.35068961171031 39.0 35.0 41.0 25.0 41.0 42-43 35.24431688420255 39.0 35.0 41.0 25.0 41.0 44-45 35.03192773485472 39.0 35.0 41.0 24.0 41.0 46-47 34.752228593651026 39.0 35.0 41.0 23.0 41.0 48-49 34.493068333125066 39.0 34.0 41.0 21.0 41.0 50-51 33.76309147279473 38.0 33.5 39.5 18.5 40.5 52-53 33.86261521357872 38.0 33.5 40.0 18.5 40.5 54-55 34.20559160206663 38.5 34.0 40.0 19.5 41.0 56-57 34.03277635099906 38.0 34.0 40.0 17.5 41.0 58-59 33.8626257169204 38.0 34.0 40.0 16.0 41.0 60-61 33.53319810056492 38.0 33.0 40.0 13.0 41.0 62-63 33.3442219770844 38.0 33.0 40.0 13.0 41.0 64-65 33.0259860752621 37.0 33.0 40.0 11.5 41.0 66-67 32.715495714636596 37.0 32.0 40.0 8.0 41.0 68-69 32.42601096010238 36.0 32.0 39.0 8.0 41.0 70-71 32.09705276232499 36.0 32.0 39.0 7.0 41.0 72-73 31.74793434280322 35.0 31.0 39.0 6.5 40.0 74-75 31.29771721987857 35.0 31.0 38.0 2.0 40.0 76-77 30.87337009682465 35.0 31.0 37.0 2.0 39.5 78-79 30.356546705936594 35.0 30.0 37.0 2.0 39.0 80-81 30.03252992644429 35.0 30.0 36.0 2.0 39.0 82-83 29.76642749570171 34.5 30.0 36.0 2.0 37.5 84-85 29.53008291714964 34.0 30.0 35.5 2.0 37.0 86-87 29.23999058469679 34.0 29.0 35.0 2.0 37.0 88-89 28.896659506439896 34.0 29.0 35.0 2.0 36.0 90-91 28.67173125234305 34.0 29.0 35.0 2.0 36.0 92-93 28.473887615321303 34.0 29.0 35.0 2.0 36.0 94-95 28.197302041634167 34.0 29.0 35.0 2.0 35.0 96-97 28.00627157224791 34.0 28.5 35.0 2.0 35.0 98-99 27.688965620138838 34.0 27.0 35.0 2.0 35.0 100-101 27.366064349396083 34.0 26.0 35.0 2.0 35.0 102-103 27.23748944279503 34.0 25.5 35.0 2.0 35.0 104-105 26.9978225764751 34.0 25.0 35.0 2.0 35.0 106-107 26.737149565430972 33.5 25.0 35.0 2.0 35.0 108-109 26.440245681241358 33.0 25.0 35.0 2.0 35.0 110-111 26.11614245116754 33.0 24.0 35.0 2.0 35.0 112-113 25.730267452783437 33.0 21.5 35.0 2.0 35.0 114-115 25.303939507215524 32.0 19.0 35.0 2.0 35.0 116-117 24.75951117986461 32.0 14.0 35.0 2.0 35.0 118-119 24.076044732385625 32.0 2.0 34.0 2.0 35.0 120-121 22.50756913891989 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25331385690472885 1101 2 0.4158357460370148 1101 3 0.44045343156851047 1101 4 0.5359956291767602 1101 5 0.6544834584374968 1101 6 0.6616840225314888 1101 7 0.6724582276792646 1101 8 0.47719011545675016 1101 9 0.7574268596274933 1101 10-11 0.6373580699046926 1101 12-13 0.35941243099021847 1101 14-15 0.6332457497622457 1101 16-17 0.5871332604564756 1101 18-19 0.5957568845252581 1101 20-21 0.6476649810734614 1101 22-23 0.7807041008083289 1101 24-25 0.6803600120659397 1101 26-27 0.49820370124840707 1101 28-29 0.6292559484061329 1101 30-31 0.6569502895500392 1101 32-33 0.6380814246191804 1101 34-35 0.6008492656790381 1101 36-37 0.4981260759750441 1101 38-39 0.4763876365707773 1101 40-41 0.7866430980413526 1101 42-43 0.794489906234233 1101 44-45 0.8207280970166124 1101 46-47 0.8736366221803777 1101 48-49 0.9473822379430246 1101 50-51 0.5740200525078833 1101 52-53 0.605604187243884 1101 54-55 0.7731350567786492 1101 56-57 0.9032922808363324 1101 58-59 0.8943359797550343 1101 60-61 0.8742071267298641 1101 62-63 0.7763005696633698 1101 64-65 0.9123028171712448 1101 66-67 0.7583029831236203 1101 68-69 0.7702709752250669 1101 70-71 0.4754919347562776 1101 72-73 0.22136096133300853 1101 74-75 0.47421270335132704 1101 76-77 0.366268232247279 1101 78-79 0.4493405172322049 1101 80-81 0.5367798059822277 1101 82-83 0.4142971053637048 1101 84-85 0.4952217325806245 1101 86-87 0.566435686592218 1101 88-89 0.573653301885912 1101 90-91 0.6376453939451139 1101 92-93 0.6711606586647356 1101 94-95 0.627945981926981 1101 96-97 0.5154353347262131 1101 98-99 0.6022675143890552 1101 100-101 0.7101844951233787 1101 102-103 0.6610639710305897 1101 104-105 0.5280844168277277 1101 106-107 0.5766294405958909 1101 108-109 0.45348116618575673 1101 110-111 0.5219590580334632 1101 112-113 0.4664533407859075 1101 114-115 0.5039321543480533 1101 116-117 0.32441780125267883 1101 118-119 0.12327405575622308 1101 120-121 0.1095429655652218 1102 1 0.7068036472748354 1102 2 0.72470956397569 1102 3 0.8439891450669705 1102 4 1.0657222212397741 1102 5 1.1364073803461352 1102 6 1.0123309875637787 1102 7 0.8932933673223147 1102 8 0.7967856980191925 1102 9 0.8049767688833924 1102 10-11 0.9470416044425605 1102 12-13 0.8530669568063658 1102 14-15 0.9818133155425315 1102 16-17 1.1295912132401469 1102 18-19 1.120467936201635 1102 20-21 1.1270120644037362 1102 22-23 1.1400147395439433 1102 24-25 0.9875504718115664 1102 26-27 1.0646408747349625 1102 28-29 0.9401525883026736 1102 30-31 1.0715545791376115 1102 32-33 0.9177995668265737 1102 34-35 0.8805931248383132 1102 36-37 0.8730233918600376 1102 38-39 0.8711252134460921 1102 40-41 0.9746002168610204 1102 42-43 0.8675671966961858 1102 44-45 0.8880859373804668 1102 46-47 0.8259735583362087 1102 48-49 1.0658874217457566 1102 50-51 0.8046768634588943 1102 52-53 0.8788100624652344 1102 54-55 1.0326220403271478 1102 56-57 1.0679843754453273 1102 58-59 0.8621765640441765 1102 60-61 1.0583147852381316 1102 62-63 0.8219679980416572 1102 64-65 0.8189943680503973 1102 66-67 0.9570355047809684 1102 68-69 0.777772977473461 1102 70-71 0.5249647372427404 1102 72-73 0.4809471388387543 1102 74-75 0.6244290931321466 1102 76-77 0.7168130642421069 1102 78-79 0.8056738677101727 1102 80-81 0.5562600296462321 1102 82-83 0.46669135358372316 1102 84-85 0.4603407285908254 1102 86-87 0.49776775122258954 1102 88-89 0.6594391751968587 1102 90-91 0.6192078089873263 1102 92-93 0.5897554844140167 1102 94-95 0.5500155352602683 1102 96-97 0.4140414759343223 1102 98-99 0.5755461318457264 1102 100-101 0.6286558795036363 1102 102-103 0.6028951649584506 1102 104-105 0.6300367008604368 1102 106-107 0.5418013400499575 1102 108-109 0.6064874778605152 1102 110-111 0.4423768482860062 1102 112-113 0.3855911616048857 1102 114-115 0.4638776344100677 1102 116-117 0.40961732806076157 1102 118-119 0.4074632737404542 1102 120-121 0.2528179826486223 1103 1 0.6671375159853561 1103 2 0.849338687380385 1103 3 0.8100069264780885 1103 4 1.018385254230047 1103 5 1.1429647125570526 1103 6 1.0610045234692507 1103 7 1.082987989592688 1103 8 0.9495292655154657 1103 9 1.003527451679247 1103 10-11 1.0318087305611172 1103 12-13 0.985289268511373 1103 14-15 1.0239629486546775 1103 16-17 1.061841636046303 1103 18-19 1.0861875879886682 1103 20-21 1.114894356385392 1103 22-23 1.251833102323019 1103 24-25 1.168447535037231 1103 26-27 1.2574328844963532 1103 28-29 1.2623431876465432 1103 30-31 1.1922654708977234 1103 32-33 1.1842639348662303 1103 34-35 1.2412574889272179 1103 36-37 1.0665487214816949 1103 38-39 0.9226923693472173 1103 40-41 1.1037459584850495 1103 42-43 1.1130998868677793 1103 44-45 1.1244720349974031 1103 46-47 1.229958800767868 1103 48-49 1.3132072181909962 1103 50-51 1.033659408744363 1103 52-53 1.0712066933877438 1103 54-55 1.2761053552489798 1103 56-57 1.2276730563964833 1103 58-59 1.024842286583052 1103 60-61 1.2091528759321406 1103 62-63 1.2104656952106367 1103 64-65 1.2851487542179072 1103 66-67 1.1861496404101928 1103 68-69 1.1878350802142208 1103 70-71 1.0201676158195028 1103 72-73 0.9437642054681454 1103 74-75 0.9211868954822329 1103 76-77 1.0489785459372207 1103 78-79 0.9701048154493535 1103 80-81 1.027701582108385 1103 82-83 0.9396018250448783 1103 84-85 0.9087191739101748 1103 86-87 0.9031085565372585 1103 88-89 0.9140283504475448 1103 90-91 1.0737099158003929 1103 92-93 1.183365451242917 1103 94-95 1.1100757386022941 1103 96-97 1.0757412402569244 1103 98-99 1.099762098039463 1103 100-101 1.167933621741554 1103 102-103 1.0468314484885362 1103 104-105 1.2742410275387854 1103 106-107 1.29461070271811 1103 108-109 1.1775070599016253 1103 110-111 1.13430789859272 1103 112-113 1.3366927368902104 1103 114-115 1.1628220245739165 1103 116-117 1.1802096213643942 1103 118-119 0.9472497509332349 1103 120-121 0.940994605456904 1104 1 0.7768076045364083 1104 2 0.9834915439883787 1104 3 0.8711664241041639 1104 4 0.9177978991563691 1104 5 1.067526217539374 1104 6 1.0960735519949054 1104 7 1.0722633486666524 1104 8 0.9556571875198827 1104 9 0.9738394016557947 1104 10-11 1.1250382878804785 1104 12-13 1.0342148453246551 1104 14-15 1.2803963960508185 1104 16-17 1.1178378322940503 1104 18-19 1.2353651416212017 1104 20-21 1.3416509485816022 1104 22-23 1.4327005581003576 1104 24-25 1.2188338808353762 1104 26-27 1.0899037499681583 1104 28-29 1.1562179710578135 1104 30-31 1.4122482619662406 1104 32-33 1.3009771222439426 1104 34-35 1.4795904615384572 1104 36-37 1.2202876992450413 1104 38-39 1.1538228723804025 1104 40-41 1.1188197883461228 1104 42-43 1.3133706150164173 1104 44-45 1.1739826968229323 1104 46-47 1.1701229355386147 1104 48-49 1.2958595952873821 1104 50-51 1.058161923226109 1104 52-53 1.0411840425281724 1104 54-55 1.0398602172230227 1104 56-57 1.3049580753592664 1104 58-59 1.1276992366404528 1104 60-61 1.1421176507980206 1104 62-63 1.1845913483974968 1104 64-65 1.0878978297120696 1104 66-67 1.1195393521186077 1104 68-69 1.2138471855500441 1104 70-71 0.9243270213992503 1104 72-73 0.8560152916769859 1104 74-75 1.0348420302368133 1104 76-77 0.9935658798651268 1104 78-79 1.1144870975265668 1104 80-81 1.152869504461087 1104 82-83 1.1899608368156116 1104 84-85 1.190274271721833 1104 86-87 1.204262214197069 1104 88-89 1.3010913947180924 1104 90-91 1.2502278716753743 1104 92-93 1.0819862178893054 1104 94-95 1.0913962426678836 1104 96-97 1.006904114988096 1104 98-99 1.149684014145457 1104 100-101 1.038024225179857 1104 102-103 1.031462510640953 1104 104-105 1.097825690879347 1104 106-107 1.0536516110909702 1104 108-109 1.0812224041486687 1104 110-111 0.9314640713562135 1104 112-113 0.9239136281723042 1104 114-115 0.8018657751880767 1104 116-117 0.9169277406837963 1104 118-119 1.071303010928034 1104 120-121 1.0300324787887227 1105 1 0.6683360310141033 1105 2 0.9731385489760171 1105 3 0.8732211730988375 1105 4 1.09792045550698 1105 5 1.1796375408360689 1105 6 1.1230134523443738 1105 7 1.0956855698662906 1105 8 1.031816474985341 1105 9 1.1766197997977699 1105 10-11 1.0132152046449363 1105 12-13 1.0609531054539545 1105 14-15 1.1999330006212432 1105 16-17 1.2994124538751137 1105 18-19 1.2359096660826197 1105 20-21 1.1905629861512352 1105 22-23 1.3016960894207372 1105 24-25 1.3136015887452999 1105 26-27 1.046657195427521 1105 28-29 1.2070335434348252 1105 30-31 1.2670407802512642 1105 32-33 1.3005597813128276 1105 34-35 1.4278897173550291 1105 36-37 1.269719591505897 1105 38-39 1.1987991868445533 1105 40-41 1.2163510218090678 1105 42-43 1.3081976326329325 1105 44-45 1.3584640053177708 1105 46-47 1.3324899680336912 1105 48-49 1.3595024894988015 1105 50-51 1.187208788120131 1105 52-53 1.4803411033231768 1105 54-55 1.4467999002739944 1105 56-57 1.4022732885546532 1105 58-59 1.2291210718157117 1105 60-61 1.2918324784803232 1105 62-63 1.0930543578026786 1105 64-65 1.1501423442033243 1105 66-67 1.294489245642346 1105 68-69 1.2316017507357628 1105 70-71 1.079364703514564 1105 72-73 1.1435839643922243 1105 74-75 1.1456092984368347 1105 76-77 1.2205657929457239 1105 78-79 1.4408052046266029 1105 80-81 1.396355342990411 1105 82-83 1.2024238641159748 1105 84-85 1.239270608324869 1105 86-87 1.3517692252690665 1105 88-89 1.2345018690167961 1105 90-91 1.1367676999328715 1105 92-93 1.042743707657106 1105 94-95 1.0601877051378779 1105 96-97 0.8816714777768766 1105 98-99 1.0375742886318022 1105 100-101 1.2737860609175335 1105 102-103 0.9801915700963768 1105 104-105 0.8931608674151974 1105 106-107 1.0241070048230583 1105 108-109 1.0851756389563079 1105 110-111 0.9619309179971616 1105 112-113 0.9103749733266788 1105 114-115 1.0467899380136707 1105 116-117 1.0691130605088333 1105 118-119 1.171478894885528 1105 120-121 1.0542890622939787 1106 1 0.696601064088906 1106 2 1.0047015540968403 1106 3 0.8971246788920233 1106 4 1.2775634921405512 1106 5 1.2729424402533596 1106 6 1.1511154884482053 1106 7 1.245437678639604 1106 8 1.104869000759237 1106 9 1.135636248908753 1106 10-11 1.1288883821350524 1106 12-13 1.140232794673068 1106 14-15 1.3411298058806196 1106 16-17 1.2466455802928422 1106 18-19 1.3919035401131339 1106 20-21 1.4299114736751761 1106 22-23 1.3090740793964315 1106 24-25 1.266468637549309 1106 26-27 1.2001938901206088 1106 28-29 1.2949968962825196 1106 30-31 1.2819590780195682 1106 32-33 1.4420010820286606 1106 34-35 1.409384467494256 1106 36-37 1.142818639331928 1106 38-39 1.2350306968756612 1106 40-41 1.3196557853955397 1106 42-43 1.1839365522346768 1106 44-45 0.8892198358552506 1106 46-47 1.2776489579957868 1106 48-49 1.307494570562632 1106 50-51 1.3378227433774583 1106 52-53 1.3825863865983266 1106 54-55 1.3068636049234854 1106 56-57 1.382833522726969 1106 58-59 1.3154147244420074 1106 60-61 1.4063473587454567 1106 62-63 1.3980376506131549 1106 64-65 1.329171086002539 1106 66-67 1.307457692145384 1106 68-69 1.0824829531100804 1106 70-71 1.1045178527518686 1106 72-73 1.2719026506366369 1106 74-75 1.4096542041023348 1106 76-77 1.4510010627736314 1106 78-79 1.3662711043019655 1106 80-81 1.4108114764305988 1106 82-83 1.3198777835139701 1106 84-85 1.2442725778716017 1106 86-87 1.2756204362007288 1106 88-89 1.5001577280787473 1106 90-91 1.2612488262419426 1106 92-93 1.0766902039013821 1106 94-95 1.3013872270134286 1106 96-97 1.1656342737182541 1106 98-99 1.2457560574206 1106 100-101 1.2163907525136857 1106 102-103 1.0576844050211953 1106 104-105 1.032454683038459 1106 106-107 1.045269784464736 1106 108-109 0.9635503554094917 1106 110-111 0.8850778461834139 1106 112-113 0.839927342895141 1106 114-115 0.7821351520151119 1106 116-117 0.6965311884886205 1106 118-119 0.5042518129387972 1106 120-121 0.5798371396088733 1107 1 0.7292583795678276 1107 2 1.023703073370413 1107 3 1.0304003033841234 1107 4 1.0413341171626342 1107 5 1.1006497504324884 1107 6 1.201604835867208 1107 7 1.1458404627967624 1107 8 0.9369892706952854 1107 9 1.0441494807216358 1107 10-11 1.1003799201345572 1107 12-13 0.9677581453426072 1107 14-15 1.1056035365805386 1107 16-17 1.2865070790738926 1107 18-19 1.2115825494054349 1107 20-21 1.1530159149004788 1107 22-23 1.28180400201947 1107 24-25 1.2609771702136925 1107 26-27 1.1553550037834626 1107 28-29 1.1920148802418211 1107 30-31 1.3439366069034264 1107 32-33 1.2926299906249241 1107 34-35 1.3593447190576953 1107 36-37 1.2720567059679624 1107 38-39 1.177489093512058 1107 40-41 1.3073708686022698 1107 42-43 1.1926043613008517 1107 44-45 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112-113 0.2971304587641903 2315 114-115 0.45781060926205797 2315 116-117 0.4647582764068616 2315 118-119 0.6284697372676931 2315 120-121 0.5460502894217463 2316 1 -1.2062271187868845 2316 2 -3.2924194493397714 2316 3 -1.4047206602893425 2316 4 -3.803932302358202 2316 5 -4.09842003517555 2316 6 -1.6631899018142917 2316 7 -7.757540270894381 2316 8 -3.3252501439661692 2316 9 -1.9138502654059977 2316 10-11 -0.9695353906475361 2316 12-13 -0.5675343449174832 2316 14-15 -4.2739986324008115 2316 16-17 -3.3666622570498674 2316 18-19 -5.165927450887498 2316 20-21 -1.5040838862328343 2316 22-23 -3.3232524520345166 2316 24-25 -0.9277543141910627 2316 26-27 -3.612057223100315 2316 28-29 -2.268167191812367 2316 30-31 -0.9111549987090939 2316 32-33 -0.5697850190905811 2316 34-35 -0.5746409794656202 2316 36-37 -0.6712604294403874 2316 38-39 -0.6824732813011991 2316 40-41 -0.3953991067590863 2316 42-43 -0.7747382134206191 2316 44-45 -0.8541386145947172 2316 46-47 -0.5749666097134494 2316 48-49 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112-113 -0.7888315327851565 2316 114-115 -0.3989761968208505 2316 116-117 -0.20232216053454977 2316 118-119 -0.23585255570113262 2316 120-121 -0.8852735807420054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30160.0 3 5187.0 4 4749.0 5 5084.0 6 5889.0 7 6467.0 8 7501.0 9 8275.0 10 10274.0 11 12095.0 12 12413.0 13 13834.0 14 17486.0 15 17622.0 16 11851.0 17 10828.0 18 10783.0 19 11550.0 20 12353.0 21 13539.0 22 15057.0 23 17033.0 24 19780.0 25 22910.0 26 26862.0 27 31508.0 28 36874.0 29 43492.0 30 51512.0 31 62993.0 32 78199.0 33 100724.0 34 136241.0 35 191533.0 36 283157.0 37 358981.0 38 150791.0 39 965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40311076116562 20.169380573096305 14.06437282074834 22.36313584498974 2 14.724584107533714 20.51427937356129 41.62063166048032 23.14050485842468 3 17.681697456882997 27.27259868410315 30.543245392892153 24.502458466121695 4 11.701970859967616 17.251673656438268 40.14540102760792 30.900954455986195 5 11.406316119255886 37.23923517324128 36.14793090581953 15.206517801683297 6 27.51959005714503 38.324489135371984 19.771637459507534 14.384283347975444 7 25.466346532957367 32.68904158384612 24.18198676865264 17.66262511454388 8 24.143980318861416 35.16116542445068 21.76045913955924 18.93439511712866 9 24.452570620700907 17.800440444794866 23.197955466137408 34.549033468366815 10-11 22.79261265939097 26.961699952833456 29.52997929837391 20.715708089401662 12-13 24.026059992024116 24.64401432179567 29.69478242880626 21.635143257373954 14-15 21.43995585259006 26.430298606665335 27.89009904623653 24.23964649450808 16-17 19.877495984644987 29.666723494782282 28.837033006802788 21.618747513769943 18-19 19.681697555015752 28.609549671723745 31.325107863752365 20.38364490950814 20-21 20.71231849984621 27.87977796409798 30.6457354687695 20.76216806728631 22-23 20.24084880838542 27.764797878359687 30.672292326971657 21.32206098628324 24-25 20.197269995533272 27.906546187392088 30.868994421077726 21.02718939599691 26-27 20.138454833616855 27.465338033863063 31.150869441218955 21.245337691301128 28-29 20.132573990880168 27.787479117040032 31.245368218630144 20.83457867344966 30-31 20.382770356824064 28.23405295763248 30.691716170414374 20.69146051512908 32-33 20.01904156374695 28.083478302488917 30.70206044295975 21.195419690804382 34-35 20.161841932421176 27.50380005444773 31.05171419239086 21.282643820740233 36-37 20.71778362152535 27.876416706831854 30.60307075999787 20.80272891164493 38-39 20.725447924958615 28.010794637274678 30.629388924863154 20.63436851290355 40-41 20.131097424153204 28.064912264000835 30.777160217643484 21.026830094202474 42-43 20.104959436735097 28.354931083653423 30.308288428488634 21.23182105112285 44-45 20.317300536293704 28.365104989627888 30.03603660925771 21.281557864820705 46-47 20.370269162825387 28.623439323010345 29.725100066316624 21.281191447847647 48-49 20.62929694881796 28.461493492633416 29.776045440764815 21.133164117783814 50-51 20.503222733197497 28.49088645962652 29.684926374426514 21.320964432749474 52-53 20.235786993948302 28.823395220689697 29.3904716691532 21.5503461162088 54-55 20.322025099455008 29.10300000734236 29.217676816982934 21.357298076219696 56-57 20.475676005100766 28.517135093824642 29.239698143966837 21.76749075710776 58-59 20.314492010537467 28.188570084090298 29.683399951855137 21.81353795351709 60-61 20.620089329555004 28.436384587700225 29.543578149487043 21.399947933257728 62-63 20.38142844362043 28.62315007911868 29.359297783997167 21.636123693263723 64-65 20.271896570757658 28.648386147729493 29.714616819868382 21.365100461644463 66-67 20.33829864701751 28.392456015220883 29.428735589364635 21.840509748396975 68-69 20.491027090061973 28.84344265594218 29.251104412537384 21.414425841458467 70-71 20.658114651607637 28.40311623158752 29.35246387121723 21.586305245587614 72-73 20.375092259653066 28.301726186947292 29.69240618070328 21.630775372696366 74-75 20.361301671638284 28.64002336768862 29.557270418986576 21.44140454168652 76-77 20.502307722437894 28.531517112615983 29.387474322371745 21.578700842574378 78-79 20.480013897030737 28.781583820414642 29.31481158278304 21.423590699771587 80-81 20.27468316257154 28.787242891094582 29.251326302541774 21.6867476437921 82-83 20.079543753147586 28.592739795015586 29.538227007309203 21.789489444527625 84-85 20.371545433964894 28.72250169789635 29.17214492198384 21.73380794615492 86-87 20.665635285530897 28.592561922993532 28.96887004461671 21.77293274685886 88-89 20.511680769697406 28.04293313297291 29.2153354069404 22.230050690389284 90-91 20.30743891948303 27.693416422678318 29.719521770144752 22.2796228876939 92-93 20.228740079837333 28.061557712055162 29.69119137158362 22.01851083652388 94-95 19.936317981411662 27.98872589993828 29.82027754936839 22.25467856928167 96-97 19.771085812216917 28.225622611226726 29.662354973408956 22.340936603147398 98-99 19.83627766124318 28.546092728640332 29.366582159430216 22.251047450686272 100-101 19.766082953371413 28.340755708135916 29.25198406921733 22.641177269275342 102-103 19.683365064287482 28.46556287013213 29.55472481115798 22.296347254422408 104-105 19.98381769202212 28.195237505966375 29.572338316305647 22.248606485705853 106-107 19.943205678017918 27.97358337848158 29.751635672121445 22.331575271379055 108-109 20.103197366867946 27.938135974443256 29.424168266320137 22.53449839236866 110-111 20.00584213029963 27.8838242889559 29.241031663639077 22.869301917105393 112-113 20.299393982582405 27.622600824070542 29.12163784903935 22.956367344307704 114-115 20.176212604053674 27.597329828520202 29.234662181202175 22.991795386223952 116-117 20.529718258125076 27.743507421061942 28.616458296488766 23.110316024324216 118-119 20.751150256986634 27.428803234840153 28.677183379708193 23.14286312846502 120-121 20.859959541492003 27.658479490169483 28.557548378708393 22.92401258963012 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4965.0 1 5838.5 2 4110.0 3 1363.0 4 1088.5 5 1000.5 6 1067.5 7 1291.5 8 1622.0 9 1763.5 10 1712.0 11 1692.5 12 1795.5 13 1918.0 14 2039.0 15 2207.5 16 2416.5 17 2657.5 18 2919.0 19 3202.0 20 3588.0 21 4072.0 22 4784.5 23 5932.0 24 7509.5 25 9581.5 26 12273.0 27 15761.0 28 20186.0 29 25394.5 30 31029.5 31 36401.5 32 41524.5 33 45935.0 34 49931.5 35 54583.5 36 58985.0 37 63494.5 38 67570.0 39 69273.0 40 70095.0 41 71143.5 42 72189.0 43 74236.5 44 74811.5 45 74319.5 46 74712.5 47 75076.5 48 80118.0 49 78587.5 50 71184.0 51 64177.5 52 52971.0 53 44701.5 54 38982.0 55 33800.0 56 28806.0 57 23868.5 58 19544.0 59 15992.5 60 12817.5 61 9844.5 62 7252.0 63 5025.0 64 3412.5 65 2280.5 66 1439.0 67 929.5 68 624.5 69 411.0 70 278.5 71 204.5 72 165.0 73 124.0 74 85.5 75 66.0 76 49.5 77 32.0 78 27.0 79 31.0 80 28.0 81 19.5 82 15.0 83 13.5 84 14.0 85 10.5 86 6.0 87 4.5 88 3.5 89 5.5 90 4.5 91 2.5 92 2.0 93 0.5 94 1.0 95 2.0 96 2.5 97 1.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19202263120020338 2 0.974710107769672 3 0.9918386341993114 4 0.9648531255790304 5 0.970508771098251 6 0.9828434646592178 7 0.9553731864230036 8 0.9498252674851014 9 0.9427153131180813 10-11 0.9331545790260656 12-13 0.9295457385518963 14-15 0.9296534651332147 16-17 0.9519528674661415 18-19 0.9823317633979549 20-21 0.9706434293248991 22-23 0.947859257376039 24-25 0.9370865992441904 26-27 0.9409108928809966 28-29 0.9712359255221508 30-31 0.9770262292680194 32-33 0.9664420926534781 34-35 0.9744677229617053 36-37 0.9862368519707501 38-39 0.9720438748820395 40-41 0.9816315406193846 42-43 0.9764068014254381 44-45 0.9548614851617407 46-47 0.950498558618342 48-49 0.9520067307568008 50-51 0.9471859662427985 52-53 0.950498558618342 54-55 0.9645030141897453 56-57 1.0120104365511982 58-59 0.9883644519517901 60-61 0.9978982543984762 62-63 0.9619175762381017 64-65 0.9528954750526782 66-67 0.9567467003348142 68-69 0.970508771098251 70-71 0.9652840319043043 72-73 0.9690544622504513 74-75 0.9757873735828568 76-77 0.9897918291542601 78-79 1.087419043474139 80-81 1.0497955349486574 82-83 1.0679474639008226 84-85 1.0225407098750803 86-87 0.9998911961528684 88-89 0.9981406392064429 90-91 1.006758765711922 92-93 0.993212148111122 94-95 0.988741494986405 96-97 0.9979251860438059 98-99 1.036006532539891 100-101 1.0221905984857953 102-103 0.9933198746924407 104-105 0.9762452115534604 106-107 0.9783728115345005 108-109 0.976703049524064 110-111 0.9793154191210373 112-113 0.9823856266886142 114-115 0.9866677582960239 116-117 1.016777337774541 118-119 1.0131954289457015 120-121 0.9896033076369527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1856552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.24281598316611 #Duplication Level Percentage of deduplicated Percentage of total 1 82.82252575471496 55.69219858585333 2 11.516197759536759 15.487631335405602 3 2.6054506340506576 5.255935126160755 4 0.9933063847245114 2.6717087377173723 5 0.47765266358527747 1.6059355080666982 6 0.32263960898703575 1.3017117513597547 7 0.235531591061172 1.1086465205164102 8 0.16044677423130846 0.8631114331782777 9 0.11894568110828163 0.7198418292080886 >10 0.6807675708215712 8.44203658880621 >50 0.04443168284836926 2.0053423860439348 >100 0.019504251582711497 2.290381369901615 >500 0.0012998213736594446 0.588445069854586 >1k 0.0012998213736594435 1.9670737579273831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4903 0.2640917141022713 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4215 0.22703377012871173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3551 0.19126854513097397 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2419 0.13029530010471024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2257 0.12156944701791278 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2190 0.11796060654374345 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2049 0.11036588256079011 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2018 0.10869612055035356 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.3863290659243584E-5 0.0 0.0 0.0 6 0.0 5.3863290659243584E-5 0.0 0.0 0.0 7 0.0 5.3863290659243584E-5 0.0 5.3863290659243584E-5 0.0 8 0.0 5.3863290659243584E-5 0.0 5.3863290659243584E-5 0.0 9 0.0 5.3863290659243584E-5 0.0 5.3863290659243584E-5 0.0 10-11 0.0 5.3863290659243584E-5 0.0 8.079493598886538E-5 0.0 12-13 0.0 5.3863290659243584E-5 0.0 1.0772658131848717E-4 0.0 14-15 0.0 8.079493598886538E-5 0.0 1.6158987197773076E-4 0.0 16-17 0.0 1.0772658131848717E-4 0.0 3.231797439554615E-4 0.0 18-19 0.0 1.0772658131848717E-4 0.0 5.11701261262814E-4 0.0 20-21 0.0 1.0772658131848717E-4 0.0 6.46359487910923E-4 0.0 22-23 0.0 1.0772658131848717E-4 0.0 9.426075865367628E-4 0.0 24-25 0.0 1.0772658131848717E-4 0.0 0.0014004455571403332 0.0 26-27 0.0 1.0772658131848717E-4 0.0 0.0018582835277439038 0.0 28-29 0.0 1.0772658131848717E-4 0.0 0.003662703764828564 0.0 30-31 0.0 1.0772658131848717E-4 0.0 0.008645058150808595 0.0 32-33 0.0 1.0772658131848717E-4 0.0 0.018205792242824335 0.0 34-35 0.0 1.0772658131848717E-4 0.0 0.026500739004347844 0.0 36-37 0.0 1.3465822664810896E-4 0.0 0.03428398450460854 0.0 38-39 0.0 1.6158987197773076E-4 0.0 0.04187870848756189 0.0 40-41 0.0 1.6158987197773076E-4 0.0 0.04904252614524129 0.0 42-43 0.0 1.6158987197773076E-4 0.0 0.058657123527916265 0.0 44-45 0.0 1.6158987197773076E-4 0.0 0.06541696650565133 0.0 46-47 0.0 1.6158987197773076E-4 0.0 0.0761088297015112 0.0 48-49 0.0 1.6158987197773076E-4 0.0 0.08755477896660045 0.0 50-51 0.0 1.6158987197773076E-4 0.0 0.09716937634927544 0.0 52-53 0.0 1.6158987197773076E-4 0.0 0.11621004959731804 0.0 54-55 0.0 1.6158987197773076E-4 0.0 0.15356424166950344 0.0 56-57 0.0 1.6158987197773076E-4 0.0 0.2282187625232151 0.0 58-59 0.0 1.6158987197773076E-4 0.0 0.3079364326988956 0.0 60-61 0.0 1.6158987197773076E-4 0.0 0.3712527308688365 0.0 62-63 0.0 1.6158987197773076E-4 0.0 0.46691393507965306 0.0 64-65 0.0 1.6158987197773076E-4 0.0 0.600791144013203 0.0 66-67 0.0 1.6158987197773076E-4 0.0 0.8684378352989843 0.0 68-69 0.0 1.6158987197773076E-4 0.0 1.1435176606957413 0.0 70-71 0.0 1.6158987197773076E-4 0.0 1.299640408671559 0.0 72-73 0.0 1.8852151730735253E-4 0.0 1.4486532022803562 0.0 74-75 0.0 2.1545316263697433E-4 0.0 1.6196960817687842 5.3863290659243584E-5 76-77 0.0 2.1545316263697433E-4 0.0 1.7965561966484107 5.3863290659243584E-5 78-79 0.0 2.1545316263697433E-4 0.0 1.9063026513666195 5.3863290659243584E-5 80-81 0.0 2.1545316263697433E-4 0.0 2.026013814856788 5.3863290659243584E-5 82-83 0.0 2.1545316263697433E-4 0.0 2.2269777523064263 5.3863290659243584E-5 84-85 0.0 2.1545316263697433E-4 0.0 2.649885378917477 5.3863290659243584E-5 86-87 0.0 2.4238480796659614E-4 0.0 3.256951596292482 5.3863290659243584E-5 88-89 0.0 2.693164532962179E-4 0.0 4.0008305719419655 5.3863290659243584E-5 90-91 0.0 2.693164532962179E-4 0.0 4.7317823578332305 5.3863290659243584E-5 92-93 0.0 2.693164532962179E-4 0.0 5.372378473643614 5.3863290659243584E-5 94-95 0.0 2.693164532962179E-4 0.0 6.012355161611417 5.3863290659243584E-5 96-97 0.0 2.693164532962179E-4 0.0 6.77697150416471 5.3863290659243584E-5 98-99 0.0 2.693164532962179E-4 0.0 7.46838763471209 5.3863290659243584E-5 100-101 0.0 2.693164532962179E-4 0.0 7.933928055879932 5.3863290659243584E-5 102-103 0.0 2.9624809862583974E-4 0.0 8.602182971443838 5.3863290659243584E-5 104-105 0.0 3.231797439554615E-4 0.0 9.42809573876735 5.3863290659243584E-5 106-107 5.3863290659243584E-5 3.231797439554615E-4 0.0 10.20701278499067 5.3863290659243584E-5 108-109 5.3863290659243584E-5 3.231797439554615E-4 0.0 10.948198596107193 5.3863290659243584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 410 0.0 53.23627 4 TATAACG 445 0.0 51.70806 2 TACCTGG 2460 0.0 51.445316 2 ACCTGGG 2810 0.0 45.585854 3 GTACCTG 3060 0.0 42.893486 1 ATAACGC 565 0.0 39.65041 3 GTACACG 295 0.0 39.028862 1 GTACCCG 280 0.0 32.895756 1 TACACGG 360 0.0 31.958456 2 GTATAAC 855 0.0 30.972027 1 CCTGGGG 2065 0.0 28.371872 4 ACACGGG 410 0.0 28.020615 3 TACCCGG 380 0.0 24.221146 2 TCACGCA 360 0.0 23.932972 4 GTATACG 320 3.092282E-11 23.386826 1 TATCACG 380 0.0 22.707325 2 AGTACTT 6510 0.0 22.240856 12-13 GAGTACT 6265 0.0 22.14767 12-13 GTACATG 7320 0.0 21.705807 1 TACATGG 7205 0.0 20.91826 2 >>END_MODULE