FastQCFastQC Report
Wed 25 May 2016
SRR1003776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856552
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60420.3254420021631497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51230.2759416380473049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.1736552490854013No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26410.1422529506310623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24890.1340657304508573No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24310.13094165959262116No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22490.12113854069263884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG4050.053.964162
TACCTGG25300.050.7044262
TAACGCA4350.050.2140774
ATAACGC4850.047.017043
ACCTGGG29100.042.609193
GTACCTG30600.042.4166641
GTATAAC7350.038.1645361
CCTGGGG18750.032.4164624
TATCACG2950.028.9903282
GTACACG3100.026.0724931
CACGCAG3800.023.7425255
TCACGCA3600.022.4234984
TAACGCC1503.0418923E-522.159694
CTGGGGG12400.020.2960325
AGTACTT77550.020.20640612-13
TACACGG3553.6379788E-1220.075462
GAGTACT75800.019.26339512-13
CTAACGC1250.00602752119.0027223
ACAACGC2552.6729867E-718.630123
GATGCGC1300.00757857918.2634818
Produced by FastQC (version 0.11.5)