##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856552 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.828726046994642 31.0 31.0 34.0 30.0 34.0 2 32.03312538512253 33.0 31.0 34.0 30.0 34.0 3 32.060917765836884 33.0 31.0 34.0 30.0 34.0 4 35.846066256156575 37.0 35.0 37.0 35.0 37.0 5 35.699383588501696 37.0 35.0 37.0 33.0 37.0 6 35.75355605444933 37.0 35.0 37.0 33.0 37.0 7 35.69734540158315 37.0 35.0 37.0 33.0 37.0 8 35.688372854625136 37.0 35.0 37.0 33.0 37.0 9 37.30984103865661 39.0 37.0 39.0 34.0 39.0 10-11 37.230485060477704 39.0 37.0 39.0 33.5 39.0 12-13 37.19188527980902 39.0 37.0 39.0 33.0 39.0 14-15 38.40857756744761 40.0 38.0 41.0 33.5 41.0 16-17 38.37567032865225 40.0 38.0 41.0 33.0 41.0 18-19 38.382134462164274 40.0 38.0 41.0 33.5 41.0 20-21 38.343544646204364 40.0 38.0 41.0 34.0 41.0 22-23 38.25998840861985 40.0 38.0 41.0 33.5 41.0 24-25 38.17963057323468 40.0 37.5 41.0 33.0 41.0 26-27 38.02130535530381 40.0 37.0 41.0 33.0 41.0 28-29 37.839648714390975 40.0 37.0 41.0 33.0 41.0 30-31 37.61103486463078 39.5 37.0 41.0 32.5 41.0 32-33 37.33277575850286 39.0 36.0 41.0 32.0 41.0 34-35 37.316572334090296 39.0 36.0 41.0 32.0 41.0 36-37 37.17311015258393 39.0 36.0 41.0 31.0 41.0 38-39 36.94437107067294 39.0 36.0 41.0 31.0 41.0 40-41 36.86915502501411 39.0 36.0 41.0 30.5 41.0 42-43 36.724497078455116 39.0 36.0 41.0 30.0 41.0 44-45 36.48686839905373 39.0 35.0 41.0 30.0 41.0 46-47 36.27739002193313 39.0 35.0 41.0 30.0 41.0 48-49 36.05422821445346 39.0 35.0 41.0 29.5 41.0 50-51 35.849315020532686 39.0 35.0 41.0 28.5 41.0 52-53 35.62302914219478 39.0 35.0 40.5 28.0 41.0 54-55 35.42370345673054 39.0 35.0 40.0 27.5 41.0 56-57 35.21084919786787 38.0 34.5 40.0 27.0 41.0 58-59 34.966289928857364 38.0 34.0 40.0 26.0 41.0 60-61 34.706649207778725 38.0 34.0 40.0 26.0 41.0 62-63 34.40669746928715 38.0 34.0 40.0 26.0 41.0 64-65 33.96313704113862 37.0 33.0 40.0 24.5 41.0 66-67 33.65592399243329 36.5 33.0 39.5 23.0 41.0 68-69 33.39150694405543 36.0 33.0 39.0 23.0 41.0 70-71 33.03386600536909 36.0 32.0 39.0 22.5 40.5 72-73 32.66923145702356 35.0 32.0 38.5 22.0 40.0 74-75 32.27090892148456 35.0 32.0 37.5 21.5 40.0 76-77 31.25283913405065 34.0 30.5 36.0 20.0 39.0 78-79 31.671949398670222 35.0 31.0 37.0 20.5 39.0 80-81 31.506211245362373 35.0 32.0 36.0 20.0 39.0 82-83 31.22657350831003 35.0 31.0 36.0 20.0 37.5 84-85 30.877721981393464 35.0 31.0 35.5 18.0 37.0 86-87 30.507781090968635 34.0 31.0 35.0 17.5 37.0 88-89 30.188234964601044 34.0 31.0 35.0 12.0 36.0 90-91 29.93281604824427 34.0 31.0 35.0 7.5 36.0 92-93 29.683835141703547 34.0 30.0 35.0 4.5 36.0 94-95 29.388210510667086 34.0 30.0 35.0 2.0 35.0 96-97 29.08236720544321 34.0 30.0 35.0 2.0 35.0 98-99 28.697505914189314 34.0 29.5 35.0 2.0 35.0 100-101 27.823058551551476 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.031033913119561163 1101 2 0.048091267234894985 1101 3 0.04605271716025072 1101 4 -0.033659477487191225 1101 5 0.020602210900406703 1101 6 -0.01688646312123865 1101 7 -0.01692320483513754 1101 8 -0.06666624145015021 1101 9 -0.07693419537881141 1101 10-11 -0.011780807039059482 1101 12-13 0.03757708812059235 1101 14-15 0.1255572136049139 1101 16-17 0.049922095142910905 1101 18-19 0.001173469549897277 1101 20-21 -0.1256680610851788 1101 22-23 -0.18246606599763027 1101 24-25 -0.16549368279321897 1101 26-27 -0.11178134492801206 1101 28-29 0.015104387353034099 1101 30-31 -0.038712101379260844 1101 32-33 -0.05883086693788897 1101 34-35 0.024788295719417874 1101 36-37 0.03229605810421532 1101 38-39 -0.11149171562464488 1101 40-41 0.11207523548662834 1101 42-43 0.029574825095117774 1101 44-45 0.2507866542588175 1101 46-47 0.11009260525073472 1101 48-49 0.08632895466376311 1101 50-51 0.11477209846142244 1101 52-53 0.10794611625087924 1101 54-55 0.14094961825414742 1101 56-57 0.14145945695936035 1101 58-59 0.17685006020615646 1101 60-61 0.1265703168327832 1101 62-63 -0.13917563188259408 1101 64-65 -0.24819169291318133 1101 66-67 -0.2194529181334488 1101 68-69 -0.03659916506988736 1101 70-71 -0.14892361574722202 1101 72-73 -0.18509187950077433 1101 74-75 0.08865791588996785 1101 76-77 0.03576560468395229 1101 78-79 -0.09195377319434073 1101 80-81 -0.06118945155894906 1101 82-83 0.16986101931150088 1101 84-85 0.03823315792379489 1101 86-87 -0.10631762646190523 1101 88-89 0.11509623169025573 1101 90-91 0.051279688209849184 1101 92-93 0.11530574605525956 1101 94-95 0.13892012429959166 1101 96-97 0.04930313971614453 1101 98-99 0.012424439206437654 1101 100-101 -0.070754229497485 1102 1 0.09643579560043847 1102 2 0.08271363216579175 1102 3 0.11557519010234074 1102 4 0.04064928758478459 1102 5 -0.03971653234889061 1102 6 -0.03391108526574271 1102 7 0.0013035011172348732 1102 8 0.04835466275788036 1102 9 0.05667492164801047 1102 10-11 0.08228812908738092 1102 12-13 -0.051521863362538056 1102 14-15 0.051027282746765934 1102 16-17 0.04769133325984143 1102 18-19 0.10058712304703477 1102 20-21 -0.037179703516677876 1102 22-23 -0.022303828769764777 1102 24-25 -0.019527203003072202 1102 26-27 -0.2650124962392155 1102 28-29 -0.08366928142732633 1102 30-31 -0.10098753728159693 1102 32-33 -0.0267001072591313 1102 34-35 -0.11941987933219878 1102 36-37 -0.15271022417974933 1102 38-39 -0.05840972263435873 1102 40-41 -0.10333362730975892 1102 42-43 -0.17155745555765378 1102 44-45 -0.1907102192946013 1102 46-47 -0.3592939352314275 1102 48-49 -0.3417407309281302 1102 50-51 -0.2986190857674771 1102 52-53 -0.33228327895988485 1102 54-55 -0.3779479327572659 1102 56-57 -0.31115595704682875 1102 58-59 -0.33951106457984537 1102 60-61 -0.2200420258345801 1102 62-63 -0.44175320523101647 1102 64-65 -0.44752158262419783 1102 66-67 -0.448247821768355 1102 68-69 -0.4488639468064477 1102 70-71 -0.5083481438411539 1102 72-73 -0.6025874892497072 1102 74-75 -0.5927766491629001 1102 76-77 -0.693600950052744 1102 78-79 -0.47446551114594726 1102 80-81 -0.3142398494369303 1102 82-83 -0.5705234123805987 1102 84-85 -0.5458693571940962 1102 86-87 -0.6537462986589411 1102 88-89 -0.5772246864784023 1102 90-91 -0.5535808156954545 1102 92-93 -0.43726043835732753 1102 94-95 -0.45939872181751795 1102 96-97 -0.4433094821638406 1102 98-99 -0.6578350080995286 1102 100-101 -0.32041366357758605 1103 1 -0.05754861109577902 1103 2 -0.027590377996752125 1103 3 -0.022198380296217124 1103 4 0.03200079335211825 1103 5 0.04927561629653354 1103 6 -0.09249826690751917 1103 7 0.08169000596526388 1103 8 0.05974619265517589 1103 9 0.09425475337648237 1103 10-11 0.11076619407112531 1103 12-13 0.011630409154065546 1103 14-15 0.06468471934864084 1103 16-17 0.05613681975663809 1103 18-19 -0.032175529607641806 1103 20-21 -0.04642552066080441 1103 22-23 0.004010593136825946 1103 24-25 -0.021725795016784843 1103 26-27 -1.7340570170887304E-4 1103 28-29 -0.04552206131132408 1103 30-31 -0.1314455354976758 1103 32-33 0.01119624027309385 1103 34-35 0.09285373133361219 1103 36-37 -0.027249012181016496 1103 38-39 -0.015280759538910615 1103 40-41 0.005222480168853849 1103 42-43 0.2258741015086443 1103 44-45 0.25342025876126684 1103 46-47 0.19441756585579384 1103 48-49 0.2026257284254953 1103 50-51 0.38364478478165864 1103 52-53 0.18564035913323806 1103 54-55 0.16765131234570418 1103 56-57 0.10388272230883899 1103 58-59 0.11007843736741307 1103 60-61 0.04748099107919046 1103 62-63 -0.2173191880634917 1103 64-65 -0.2685771012892033 1103 66-67 -0.2604529021281152 1103 68-69 -0.358270529058764 1103 70-71 -0.3593267900409103 1103 72-73 -0.11877902765630921 1103 74-75 -0.12104684395004028 1103 76-77 -0.31287270538587464 1103 78-79 -0.15093545582026024 1103 80-81 -0.08637369474686096 1103 82-83 -0.0973456575306777 1103 84-85 -0.28711594017579145 1103 86-87 -0.04845107891370404 1103 88-89 -0.18721886672070198 1103 90-91 -0.13604161590453856 1103 92-93 -0.20158729904630945 1103 94-95 -0.09593905061103314 1103 96-97 -0.040653514936824564 1103 98-99 -0.19895607881338506 1103 100-101 -0.1502275376975959 1104 1 0.023913009939544594 1104 2 0.06046157344756864 1104 3 0.09301314589407639 1104 4 0.020191187447217374 1104 5 0.028199184260927268 1104 6 0.0461147939686839 1104 7 -0.025130378066940295 1104 8 -0.09442176452586892 1104 9 0.03898062324536511 1104 10-11 0.08921569235645421 1104 12-13 0.16724576372522648 1104 14-15 0.09692052462339262 1104 16-17 0.18647814992556988 1104 18-19 0.12406844540053896 1104 20-21 0.08906620574268942 1104 22-23 -0.003212711251663336 1104 24-25 0.08065008859686174 1104 26-27 -0.049273901632396644 1104 28-29 0.23586215995809567 1104 30-31 0.037650096904116026 1104 32-33 0.18153916259470293 1104 34-35 0.22895293239636771 1104 36-37 0.2512848826349128 1104 38-39 0.35636452501484683 1104 40-41 0.2747745504026895 1104 42-43 0.33169503657493493 1104 44-45 0.43297823115483425 1104 46-47 0.456904769550583 1104 48-49 0.3536931859442518 1104 50-51 0.3380983343492332 1104 52-53 0.47051130147617215 1104 54-55 0.3286776537654319 1104 56-57 0.3174269350204426 1104 58-59 0.30077401528774317 1104 60-61 0.3100356746823749 1104 62-63 0.1183572840246967 1104 64-65 0.2299996012879646 1104 66-67 0.3101498955826614 1104 68-69 0.18485286401760703 1104 70-71 0.08306962363518267 1104 72-73 0.14985973155921073 1104 74-75 0.22775431483858455 1104 76-77 0.1415032578353994 1104 78-79 0.1363272916630791 1104 80-81 0.13343588752452007 1104 82-83 0.11093838748246654 1104 84-85 0.2553398060649279 1104 86-87 0.223471495199135 1104 88-89 0.09091574605657016 1104 90-91 0.07598165564580484 1104 92-93 0.13612336576450446 1104 94-95 0.15541085852431635 1104 96-97 0.065817030860579 1104 98-99 -0.036204490703280356 1104 100-101 0.1954208789190055 1105 1 -0.0361546849324661 1105 2 -0.08606344016554246 1105 3 0.02056071509086621 1105 4 -0.07594997476738286 1105 5 -0.10382818512350411 1105 6 -0.016970793516968286 1105 7 -0.028318274796468756 1105 8 0.02850718909240868 1105 9 0.016190485017958167 1105 10-11 -0.014037841300527987 1105 12-13 0.009406848377054189 1105 14-15 0.03465428028274431 1105 16-17 0.0229424989120659 1105 18-19 -0.011484911605549541 1105 20-21 -0.06129316761958847 1105 22-23 -0.008246047397094003 1105 24-25 -0.09798301440007151 1105 26-27 -0.19132000981636565 1105 28-29 -0.0928705168781434 1105 30-31 -0.0234548787201021 1105 32-33 -0.09923349972088147 1105 34-35 -0.14587354836504574 1105 36-37 -0.23727083653363223 1105 38-39 -0.19857780140839765 1105 40-41 -0.06532571367888806 1105 42-43 0.07178622524882172 1105 44-45 0.22936538804690088 1105 46-47 0.11791081044871277 1105 48-49 0.004220006969539725 1105 50-51 -0.11780604920050308 1105 52-53 0.015332950541655066 1105 54-55 -0.018307137896435677 1105 56-57 -0.1097930886523173 1105 58-59 -0.17934732674775944 1105 60-61 -0.1675250602466889 1105 62-63 -0.24254675440389661 1105 64-65 -0.4568285309419977 1105 66-67 -0.37057822054165257 1105 68-69 -0.30060219737560345 1105 70-71 -0.29156384493451526 1105 72-73 -0.4491197324548821 1105 74-75 -0.27669455226932627 1105 76-77 -0.3409482909915731 1105 78-79 -0.3581293598438222 1105 80-81 -0.24789261155643771 1105 82-83 -0.3157380703986945 1105 84-85 -0.29877966209062023 1105 86-87 -0.3227006000951995 1105 88-89 -0.19335893713500596 1105 90-91 -0.2794793750648594 1105 92-93 -0.3366277226453036 1105 94-95 -0.21158137241541297 1105 96-97 -0.09312147877852794 1105 98-99 -0.10637017441382213 1105 100-101 0.05307598530579227 1106 1 0.024530445668855094 1106 2 0.027767531975555926 1106 3 -0.013060126788488446 1106 4 -0.009347374965699373 1106 5 0.014375180763948947 1106 6 0.002924345098378467 1106 7 -0.01054277595782338 1106 8 0.04449990856103625 1106 9 -0.008206765766466617 1106 10-11 0.0211352071503228 1106 12-13 -0.044522397354469945 1106 14-15 -0.02652085996256659 1106 16-17 -0.009083689267939121 1106 18-19 0.022384840231566727 1106 20-21 -0.03077096607383112 1106 22-23 -0.046728953089768765 1106 24-25 -0.04621178064882514 1106 26-27 -0.01602457780647626 1106 28-29 -0.11444724091068537 1106 30-31 -0.08490269931476746 1106 32-33 -0.13385643339469766 1106 34-35 -0.077011742630269 1106 36-37 -0.10916234846636286 1106 38-39 -0.04221141138381057 1106 40-41 -0.09365085188674271 1106 42-43 0.015438172383476001 1106 44-45 0.12380685175969575 1106 46-47 -0.1565271895784548 1106 48-49 -0.0774873183859981 1106 50-51 0.11698599383068853 1106 52-53 0.21795618304324904 1106 54-55 0.0073105527081409605 1106 56-57 -0.0807426139236398 1106 58-59 0.007368420117948915 1106 60-61 -0.032732677638470875 1106 62-63 -0.1629468440522288 1106 64-65 -0.1473472008867205 1106 66-67 -0.20433115788289768 1106 68-69 -0.1902967138858358 1106 70-71 -0.028413724724245526 1106 72-73 0.10656546606184492 1106 74-75 0.05479035013623701 1106 76-77 -0.12823282937056746 1106 78-79 -0.08570437240817341 1106 80-81 0.10051918670764337 1106 82-83 0.07154680755732201 1106 84-85 0.06569073358452826 1106 86-87 0.15362612733311565 1106 88-89 0.15516526112364204 1106 90-91 0.05966468709131689 1106 92-93 0.13664654692451705 1106 94-95 0.10098908981683152 1106 96-97 0.0478362642567447 1106 98-99 0.23447550820391072 1106 100-101 0.3012680772360845 1107 1 -0.05731600170421558 1107 2 -0.0037503283219351147 1107 3 0.037312625216593176 1107 4 -0.03935656127103471 1107 5 -0.13509837327626428 1107 6 0.05883714586154554 1107 7 0.16505521718778482 1107 8 0.03986082436501448 1107 9 0.1863326823485778 1107 10-11 0.11192151955537355 1107 12-13 0.07439344195585562 1107 14-15 0.14692983431012152 1107 16-17 0.17422726716777248 1107 18-19 0.15417563979345061 1107 20-21 0.03657436834301819 1107 22-23 0.040676975610367094 1107 24-25 0.13233996298259143 1107 26-27 0.11230369128160334 1107 28-29 0.0073949666291568406 1107 30-31 -0.0017860685689186084 1107 32-33 -0.04512644010776512 1107 34-35 -0.042537481951185896 1107 36-37 0.027394730288534674 1107 38-39 0.15930256239987983 1107 40-41 0.2838029515969893 1107 42-43 0.10612997183935846 1107 44-45 0.19035898404248996 1107 46-47 0.06952305244536205 1107 48-49 0.07650607454055347 1107 50-51 0.04805072195139104 1107 52-53 -0.030474195361868794 1107 54-55 0.14747590620424234 1107 56-57 0.011146055122047471 1107 58-59 -0.06483417324671592 1107 60-61 -0.2321126020281028 1107 62-63 -0.08549127397132139 1107 64-65 -0.06712337302394644 1107 66-67 -0.07506482569196038 1107 68-69 -0.1954322118895888 1107 70-71 -0.26385784725542294 1107 72-73 0.006885220151524152 1107 74-75 0.019463706317559115 1107 76-77 0.006211399156441644 1107 78-79 -0.07617711381367798 1107 80-81 0.09924988663344791 1107 82-83 0.07439986200384752 1107 84-85 0.21187753357039796 1107 86-87 0.2021898838253584 1107 88-89 0.05193592147766779 1107 90-91 -0.01902220122440923 1107 92-93 0.018027495940522442 1107 94-95 0.09424422178163994 1107 96-97 0.1582485880386777 1107 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-0.28643740770552384 2316 82-83 -0.42097689435354013 2316 84-85 -0.3491164418430408 2316 86-87 -0.3964815174524645 2316 88-89 -0.3606117202758732 2316 90-91 -0.4125499205066667 2316 92-93 -0.4634935250169647 2316 94-95 -0.39540380696452004 2316 96-97 -0.6667225258426086 2316 98-99 -0.6633415090326267 2316 100-101 -0.8054533701516924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 34.0 6 71.0 7 238.0 8 545.0 9 1084.0 10 1949.0 11 3354.0 12 5640.0 13 7978.0 14 8542.0 15 10999.0 16 13538.0 17 17685.0 18 15022.0 19 9993.0 20 8498.0 21 8215.0 22 8708.0 23 9874.0 24 11286.0 25 13750.0 26 17160.0 27 22044.0 28 27727.0 29 34798.0 30 43686.0 31 55517.0 32 69282.0 33 89213.0 34 118306.0 35 161524.0 36 236630.0 37 359988.0 38 375832.0 39 87767.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95523145745525 21.14618850610392 14.514626179732716 21.383953856708114 2 15.881288938062156 21.397714161361808 40.48114867216023 22.239848228415802 3 18.165716025466587 27.816237508520747 30.683087567660777 23.334958898351893 4 12.832928999564785 18.126882522008543 38.581467149856294 30.45872132857038 5 12.203967354536797 37.858137019593315 35.16330272462069 14.774592901249198 6 28.217685257401893 38.871197790312365 18.959393542437812 13.951723409847933 7 26.330477142573976 32.96077890627356 23.33724021734915 17.37150373380331 8 25.02520802002853 35.60751328268748 21.026181868323647 18.341096828960353 9 25.178879988279345 17.736535254601 22.791497356389694 34.29308740072995 10-11 23.53922755732131 27.48070078295679 28.86296747949963 20.117104180222263 12-13 24.707683921592285 25.017505569464255 29.34738698404354 20.927423524899922 14-15 21.878629926769236 26.90184933869854 27.724217538271024 23.495303196261194 16-17 20.37069255264598 30.026522284320613 28.52936518880161 21.073419974231804 18-19 20.159413795035096 28.634748716976414 31.34762721431988 19.858210273668607 20-21 21.118995449409528 28.11125580944352 30.86043733612016 19.909311405026795 22-23 20.705964605354442 28.01857960348 30.762860399277802 20.512595391887757 24-25 20.748112630295303 28.102337020455124 30.9774248176189 20.172125531630677 26-27 20.711160883700476 27.80717538670043 31.235989675958358 20.245674053640737 28-29 20.669997268791334 27.92224512894052 31.40262379189806 20.005133810370083 30-31 20.962222544837108 28.45826924055887 30.849833154533457 19.729675060070562 32-33 20.59791859688436 28.366093167568035 30.915838917498007 20.120149318049602 34-35 20.727125721102116 27.827734330076304 31.114766840843515 20.330373107978065 36-37 21.205730650998763 28.184284616801907 30.731432167111844 19.878552565087485 38-39 21.222866802516208 28.32511419363652 30.717838995392377 19.734180008454896 40-41 20.69915706740148 28.252503251526917 30.77672238006653 20.271617301005072 42-43 20.710351310512383 28.423964424524666 30.388507462284554 20.477176802678397 44-45 20.955443777818182 28.636244268024157 30.04022651825815 20.36808543589951 46-47 20.984315642476908 28.778769356317845 29.651717576526387 20.58519742467886 48-49 21.186272239069663 28.625177077666397 29.744846568598405 20.443704114665533 50-51 21.18107661762504 28.765759340873615 29.436800505031464 20.616363536469887 52-53 20.97921534038499 29.093702658847455 29.242878207415686 20.68420379335187 54-55 21.01037392060082 29.40358372781918 29.028448495729 20.55759385585101 56-57 21.21065205739204 29.11223876898546 28.87262942702231 20.804479746600194 58-59 21.09164159133293 28.726528571874905 29.35645543054698 20.825374406245185 60-61 21.30491564677628 29.061765482982434 29.232575408057155 20.40074346218413 62-63 21.214932882290835 28.973653875158906 29.141205748637176 20.670207493913082 64-65 20.99681609170001 29.00253710112884 29.353564742972303 20.647082064198845 66-67 21.075018824413682 28.655108454215927 29.205333606326615 21.06453911504377 68-69 21.245967403039884 29.142745851031503 28.926273244675464 20.68501350125315 70-71 21.320017087948525 28.903656185304037 29.00250985977862 20.77381686696881 72-73 21.072178331014964 28.802764270776805 29.27339591365155 20.851661484556683 74-75 21.11343352479107 29.045369477882442 29.16637378399829 20.674823213328196 76-77 21.113248000784253 28.98369305705962 28.969526923867267 20.933532018288854 78-79 21.143954420650214 29.189663042995246 28.946361314109424 20.720021222245112 80-81 20.969305722757717 29.24213656085126 28.742334510257013 21.04622320613401 82-83 20.749771685316517 29.03142057113278 28.98604041876858 21.232767324782113 84-85 20.915854716760595 28.94916293167017 28.741006787360785 21.393975564208453 86-87 21.252346516920507 28.91490593732746 28.467310359687044 21.36543718606499 88-89 21.12912182764904 28.55316503900516 28.695499014569503 21.622214118776288 90-91 20.897660053281967 28.25713774778647 29.06964468273744 21.775557516194123 92-93 20.713103411871607 28.702155129926687 28.97195707945988 21.612784378741825 94-95 20.443402225198536 28.665125960909954 29.13576749042625 21.75570432346526 96-97 20.452479572002513 28.926755471907683 28.916413204812706 21.704351751277095 98-99 20.56020118460174 29.19843169955686 28.655608193149718 21.585758922691685 100-101 20.145243403768905 29.46911793303279 28.210284308185567 22.175354355012736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 316.0 1 235.0 2 500.5 3 711.5 4 451.0 5 375.0 6 509.5 7 826.5 8 1245.5 9 1506.0 10 1548.5 11 1553.5 12 1685.0 13 1908.0 14 2172.5 15 2469.0 16 2685.5 17 2914.0 18 3213.0 19 3479.0 20 3851.0 21 4348.5 22 5075.0 23 6190.0 24 7632.0 25 9550.0 26 12178.0 27 15602.5 28 19967.5 29 25205.0 30 30584.5 31 35606.0 32 40828.5 33 45544.0 34 49737.5 35 54417.0 36 58880.0 37 63265.0 38 67427.5 39 69656.0 40 70785.0 41 71994.5 42 72967.5 43 74936.0 44 75734.5 45 75501.0 46 76594.0 47 77503.5 48 84002.0 49 82391.5 50 74045.5 51 67074.0 52 54863.0 53 45548.0 54 38928.0 55 33462.0 56 28302.5 57 23000.5 58 18514.0 59 15107.5 60 12080.5 61 9074.0 62 6542.0 63 4475.0 64 2854.0 65 1873.0 66 1162.0 67 639.5 68 364.5 69 198.5 70 119.5 71 75.5 72 47.5 73 33.5 74 20.0 75 12.5 76 10.5 77 10.0 78 6.0 79 2.5 80 1.0 81 1.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050685356510348216 2 0.0042013366714209995 3 0.0423904097488247 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012657873304922243 16-17 0.0 18-19 0.0 20-21 0.010745726486519096 22-23 0.0 24-25 0.0 26-27 0.005844167036527929 28-29 0.01244242014228527 30-31 0.010422546742563633 32-33 0.006032688553835282 34-35 0.005682577164550198 36-37 0.019283058056009203 38-39 0.04430255656722785 40-41 0.006275073361801878 42-43 0.011688334073055858 44-45 0.01020709357992666 46-47 0.004551448060706083 48-49 0.001184992394503359 50-51 0.001831351882414282 52-53 7.810177145590321E-4 54-55 0.0011580607491737372 56-57 0.010611068259870987 58-59 0.007864040436249563 60-61 0.005520987292572468 62-63 0.006032688553835282 64-65 0.06921432849712801 66-67 0.03148309339032788 68-69 0.023053488402156257 70-71 0.0147046783499735 72-73 2.4238480796659614E-4 74-75 3.501113892850833E-4 76-77 6.194278425813013E-4 78-79 5.11701261262814E-4 80-81 9.15675941207141E-4 82-83 7.271544238997884E-4 84-85 7.002227785701666E-4 86-87 0.004147473380761757 88-89 1.6158987197773076E-4 90-91 7.540860692294102E-4 92-93 2.6931645329621797E-4 94-95 0.002343053143677096 96-97 0.004982354385980031 98-99 0.007944835372238428 100-101 0.008133356889545782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1856552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.25076246634181 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9775070593254 52.02866571185234 2 12.588795779443593 16.17679454724633 3 2.9266344730407634 5.641154890594486 4 1.139358287018288 2.9281855465308047 5 0.5781747849071441 1.857408538454859 6 0.3544627250648047 1.3664700210786596 7 0.24121100134615517 1.0848593526232189 8 0.17255065504517825 0.8869208920575546 9 0.135491023369447 0.7834861402948714 >10 0.8046112954097381 9.546287914411808 >50 0.05405051194256901 2.4083209558766745 >100 0.02436177504109528 2.854757891702002 >500 0.001691290330753253 0.701932505389746 >1k 9.302096819142886E-4 1.1281232765235152 >5k 1.691290330753252E-4 0.6066318153631483 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6042 0.3254420021631497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5123 0.2759416380473049 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3224 0.1736552490854013 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2641 0.1422529506310623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2489 0.1340657304508573 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2431 0.13094165959262116 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2249 0.12113854069263884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6931645329621792E-5 0.0 12-13 0.0 0.0 0.0 5.3863290659243584E-5 0.0 14-15 2.6931645329621792E-5 0.0 0.0 8.079493598886538E-5 0.0 16-17 5.3863290659243584E-5 2.6931645329621792E-5 0.0 1.8852151730735253E-4 0.0 18-19 5.3863290659243584E-5 5.3863290659243584E-5 0.0 3.231797439554615E-4 0.0 20-21 5.3863290659243584E-5 5.3863290659243584E-5 0.0 4.3090632527394867E-4 0.0 22-23 5.3863290659243584E-5 5.3863290659243584E-5 0.0 7.002227785701666E-4 0.0 24-25 5.3863290659243584E-5 5.3863290659243584E-5 0.0 0.0012388556851626025 0.0 26-27 5.3863290659243584E-5 5.3863290659243584E-5 0.0 0.001723625301095795 0.0 28-29 5.3863290659243584E-5 5.3863290659243584E-5 0.0 0.0035280455381804547 0.0 30-31 5.3863290659243584E-5 5.3863290659243584E-5 0.0 0.008402673342842 0.0 32-33 8.079493598886538E-5 5.3863290659243584E-5 0.0 0.0174247745282653 0.0 34-35 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.02588131116176654 0.0 36-37 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.03396080476065308 0.0 38-39 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.041986435068880376 0.0 40-41 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.04928491095320788 0.0 42-43 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.05868405517324589 0.0 44-45 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.06565935131361794 0.0 46-47 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.07662053096277399 0.0 48-49 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.08871283971577418 0.0 50-51 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.09881220671438236 0.0 52-53 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.11946877868220228 0.0 54-55 1.0772658131848717E-4 5.3863290659243584E-5 0.0 0.15811568973020956 0.0 56-57 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.23656757257539784 0.0 58-59 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.319651698417281 0.0 60-61 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.3848262801149658 0.0 62-63 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.4832345121494038 0.0 64-65 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.622147938759593 0.0 66-67 1.0772658131848717E-4 1.0772658131848717E-4 0.0 0.8987628679401385 0.0 68-69 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.1830802476849558 0.0 70-71 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.3449932994066418 0.0 72-73 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.503351373944818 0.0 74-75 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.6843858938505358 0.0 76-77 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.8701603833342668 0.0 78-79 1.0772658131848717E-4 1.0772658131848717E-4 0.0 1.9861280481236183 0.0 80-81 1.0772658131848717E-4 1.0772658131848717E-4 0.0 2.1113332672610303 0.0 82-83 1.0772658131848717E-4 1.0772658131848717E-4 0.0 2.3217232805760357 0.0 84-85 1.0772658131848717E-4 1.0772658131848717E-4 0.0 2.7610322791928263 0.0 86-87 1.0772658131848717E-4 1.0772658131848717E-4 0.0 3.3906402837087244 0.0 88-89 1.0772658131848717E-4 1.0772658131848717E-4 0.0 4.160185117357337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 405 0.0 53.96416 2 TACCTGG 2530 0.0 50.704426 2 TAACGCA 435 0.0 50.214077 4 ATAACGC 485 0.0 47.01704 3 ACCTGGG 2910 0.0 42.60919 3 GTACCTG 3060 0.0 42.416664 1 GTATAAC 735 0.0 38.164536 1 CCTGGGG 1875 0.0 32.416462 4 TATCACG 295 0.0 28.990328 2 GTACACG 310 0.0 26.072493 1 CACGCAG 380 0.0 23.742525 5 TCACGCA 360 0.0 22.423498 4 TAACGCC 150 3.0418923E-5 22.15969 4 CTGGGGG 1240 0.0 20.296032 5 AGTACTT 7755 0.0 20.206406 12-13 TACACGG 355 3.6379788E-12 20.07546 2 GAGTACT 7580 0.0 19.263395 12-13 CTAACGC 125 0.006027521 19.002722 3 ACAACGC 255 2.6729867E-7 18.63012 3 GATGCGC 130 0.007578579 18.263481 8 >>END_MODULE