##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003775_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1270031 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92794191637842 31.0 31.0 34.0 30.0 34.0 2 32.106654876928204 33.0 31.0 34.0 30.0 34.0 3 32.16404560203649 33.0 31.0 34.0 30.0 34.0 4 35.87146140527278 37.0 35.0 37.0 35.0 37.0 5 35.75763898676489 37.0 35.0 37.0 35.0 37.0 6 35.840058234798995 37.0 35.0 37.0 35.0 37.0 7 35.80078990197877 37.0 35.0 37.0 33.0 37.0 8 35.80441658510698 37.0 35.0 37.0 33.0 37.0 9 37.43834126883517 39.0 37.0 39.0 34.0 39.0 10-11 37.34838244105852 39.0 37.0 39.0 34.0 39.0 12-13 37.3216724631131 39.0 37.0 39.0 34.0 39.0 14-15 38.48818926467149 40.0 38.0 41.0 34.0 41.0 16-17 38.51612834647344 40.0 38.0 41.0 34.0 41.0 18-19 38.548068511713495 40.0 38.0 41.0 34.0 41.0 20-21 38.48809950308299 40.0 38.0 41.0 34.0 41.0 22-23 38.38976213966431 40.0 38.0 41.0 34.0 41.0 24-25 38.27627553973092 40.0 38.0 41.0 34.0 41.0 26-27 38.081190537868764 40.0 37.0 41.0 33.5 41.0 28-29 37.936341711344056 40.0 37.0 41.0 33.0 41.0 30-31 37.73102270731974 40.0 37.0 41.0 33.0 41.0 32-33 37.42701752949338 39.0 36.0 41.0 32.0 41.0 34-35 37.330725785433586 39.0 36.0 41.0 32.0 41.0 36-37 37.15205573722216 39.0 36.0 41.0 31.5 41.0 38-39 36.932701642715806 39.0 36.0 41.0 31.0 41.0 40-41 36.95935650389636 39.0 36.0 41.0 31.0 41.0 42-43 36.82455467622444 39.0 36.0 41.0 31.0 41.0 44-45 36.54612761420784 39.0 36.0 41.0 30.0 41.0 46-47 36.32820970511743 39.0 36.0 41.0 30.0 41.0 48-49 36.05766355309437 39.0 35.0 41.0 29.0 41.0 50-51 35.80651377801014 39.0 35.0 41.0 28.0 41.0 52-53 35.648724322477165 39.0 35.0 41.0 28.0 41.0 54-55 35.47611751209222 39.0 35.0 40.5 27.5 41.0 56-57 35.196233792718445 38.5 34.5 40.0 26.5 41.0 58-59 34.942862812010105 38.0 34.0 40.0 26.0 41.0 60-61 34.558349363125785 38.0 34.0 40.0 26.0 41.0 62-63 34.325091277299535 38.0 34.0 40.0 26.0 41.0 64-65 33.89519271576836 37.0 33.5 39.5 23.5 41.0 66-67 33.52078216988404 36.5 33.0 39.0 23.0 41.0 68-69 33.195312161671644 36.0 33.0 39.0 22.5 40.5 70-71 32.73905755056373 35.5 32.0 39.0 22.0 40.0 72-73 32.32228071598252 35.0 32.0 38.0 21.0 40.0 74-75 31.949630757044517 35.0 31.5 37.0 20.0 39.5 76-77 30.95879510027708 34.0 30.5 36.0 19.5 39.0 78-79 31.362197458172282 35.0 31.0 36.0 20.0 39.0 80-81 31.1247977411575 35.0 31.0 36.0 18.0 38.5 82-83 30.816391883347727 35.0 31.0 35.5 17.5 37.0 84-85 30.49237302081603 34.0 31.0 35.0 14.0 37.0 86-87 30.24748687236768 34.0 31.0 35.0 9.5 36.5 88-89 29.933769333189503 34.0 30.5 35.0 7.0 36.0 90-91 29.643719720227303 34.0 30.0 35.0 2.0 36.0 92-93 29.39814579329166 34.0 30.0 35.0 2.0 35.5 94-95 29.078264231345536 34.0 30.0 35.0 2.0 35.0 96-97 28.752381241087818 34.0 29.0 35.0 2.0 35.0 98-99 28.453382240276028 34.0 29.0 35.0 2.0 35.0 100-101 27.568044795756954 33.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09080210238611741 1101 2 0.07274002924974354 1101 3 -0.03133462118132968 1101 4 0.03149686115731498 1101 5 -0.04521474017429483 1101 6 -0.021453999264736012 1101 7 -0.10473035180393708 1101 8 -0.08242269028134075 1101 9 0.01828375717393982 1101 10-11 0.054316425400173785 1101 12-13 0.01629107288676579 1101 14-15 0.12515885260699378 1101 16-17 0.06729093162211086 1101 18-19 0.07511659322225483 1101 20-21 0.12290392730248811 1101 22-23 0.010574517340330658 1101 24-25 0.1152453509277791 1101 26-27 -0.03089707423302457 1101 28-29 -0.006699356936529455 1101 30-31 0.01732317456713872 1101 32-33 0.12441766477381577 1101 34-35 0.08247502197455248 1101 36-37 0.09042061209982677 1101 38-39 -0.07513547164043644 1101 40-41 0.01911833085386405 1101 42-43 0.27273484889197874 1101 44-45 0.2336531811336897 1101 46-47 0.10762377872951134 1101 48-49 0.22726756763802314 1101 50-51 -0.044948863769270986 1101 52-53 0.10148558473206748 1101 54-55 0.1607376966344347 1101 56-57 0.01568068127568978 1101 58-59 0.2258147937074071 1101 60-61 0.08082278608620896 1101 62-63 -0.09730060685500774 1101 64-65 -0.08967704230241935 1101 66-67 -0.012836909992309131 1101 68-69 0.059832998424276695 1101 70-71 0.04110555403132565 1101 72-73 -0.08355238137908572 1101 74-75 -0.22563217475219588 1101 76-77 -0.2783408140080965 1101 78-79 -0.1709558413436234 1101 80-81 -0.09752122504708538 1101 82-83 0.05872345019485792 1101 84-85 -0.007281311016157588 1101 86-87 -0.16035113818893976 1101 88-89 -0.080768086924067 1101 90-91 -0.15794082859977365 1101 92-93 -0.06418159965463488 1101 94-95 -0.017040841136761742 1101 96-97 -0.14543006664558078 1101 98-99 -0.3007815363957249 1101 100-101 -0.18196560780626925 1102 1 -0.05631288222158659 1102 2 -0.03474395444274592 1102 3 0.03783606689587771 1102 4 -0.05688091569600573 1102 5 0.04938443612988408 1102 6 -0.08030456919568252 1102 7 -0.10647758535081664 1102 8 -0.1407085293694479 1102 9 0.030916879729893765 1102 10-11 -0.04242779208880165 1102 12-13 -0.06941697270771385 1102 14-15 -0.1383832474013218 1102 16-17 -0.1293430437502181 1102 18-19 -0.04632237840599629 1102 20-21 -0.08879993741170722 1102 22-23 -0.11744258058794799 1102 24-25 -0.08255188677918568 1102 26-27 -0.18424177845134437 1102 28-29 -0.23586931699976788 1102 30-31 -0.24108018677740262 1102 32-33 -0.3040227212815978 1102 34-35 -0.20099438063797948 1102 36-37 -0.2215864184351588 1102 38-39 -0.32800800679311237 1102 40-41 -0.19132606967863097 1102 42-43 -0.14668804402756308 1102 44-45 0.13131833630467327 1102 46-47 -0.20586112018054337 1102 48-49 -0.08393396743145587 1102 50-51 0.09487350082012824 1102 52-53 -0.09087650862011998 1102 54-55 -0.12356996207261517 1102 56-57 0.12021932841771132 1102 58-59 -0.0484811127739988 1102 60-61 -0.3380858169589729 1102 62-63 -0.2624209371653521 1102 64-65 -0.3672417315444605 1102 66-67 -0.46337928040582455 1102 68-69 -0.24899677110730067 1102 70-71 -0.17879574807367504 1102 72-73 -0.2074145995336778 1102 74-75 -0.23789297015946786 1102 76-77 -0.356751039484422 1102 78-79 -0.10307841809709473 1102 80-81 -0.21357497327904795 1102 82-83 -0.2940814836598733 1102 84-85 -0.40418153150038805 1102 86-87 -0.321662915375061 1102 88-89 -0.2842750342574547 1102 90-91 -0.20085049246532094 1102 92-93 -0.15594672154497502 1102 94-95 -0.3683991072154704 1102 96-97 -0.22663430576697507 1102 98-99 -0.3013129865995694 1102 100-101 -0.16082876197883067 1103 1 -0.09133259460006471 1103 2 -0.05787687859488955 1103 3 -0.13692704855593973 1103 4 -0.004843036568722425 1103 5 0.0495518793447971 1103 6 0.014613893238795583 1103 7 -0.005339643502338731 1103 8 0.018545648310762886 1103 9 0.05130519118547028 1103 10-11 0.04947473243966982 1103 12-13 0.024043221728021535 1103 14-15 -0.037010661047666815 1103 16-17 0.01079544690880141 1103 18-19 -0.1530413874109655 1103 20-21 -0.09981394888072259 1103 22-23 -0.07760957579426275 1103 24-25 -0.06718855180314875 1103 26-27 -0.2992030257072926 1103 28-29 -0.17052810244845062 1103 30-31 -0.1805878253240607 1103 32-33 -0.009599308352072455 1103 34-35 -0.06019171722684291 1103 36-37 -0.18041498794369204 1103 38-39 0.02123597869140781 1103 40-41 -0.06955537168122561 1103 42-43 -0.0403159616835822 1103 44-45 0.1809929700576376 1103 46-47 0.02814385271493336 1103 48-49 0.0593859443108542 1103 50-51 0.14355075542341922 1103 52-53 0.046975738143018475 1103 54-55 0.08552825261310204 1103 56-57 0.055883053161231544 1103 58-59 0.017621996405701168 1103 60-61 0.05313265443567872 1103 62-63 -0.06286474568647549 1103 64-65 0.0543769235337237 1103 66-67 -0.1405977630006916 1103 68-69 -0.2646692320185551 1103 70-71 -0.1334401972470971 1103 72-73 -0.10574800753511227 1103 74-75 -0.3344886647968046 1103 76-77 -0.1713611599987388 1103 78-79 -0.12512296135232504 1103 80-81 -0.06700223908255154 1103 82-83 -0.10948457939931089 1103 84-85 -0.17799178865732657 1103 86-87 -0.20574880982423593 1103 88-89 -0.4038014891653887 1103 90-91 -0.22969580629534647 1103 92-93 -0.14420553611421028 1103 94-95 -0.514919200401259 1103 96-97 -0.36793033865080815 1103 98-99 -0.45729334142238187 1103 100-101 -0.24645804606108612 1104 1 0.041719207431413 1104 2 -0.0845054198552262 1104 3 0.045200822285409004 1104 4 0.002964824436318736 1104 5 -0.034799708071020063 1104 6 -0.09863010139785189 1104 7 -0.14867120342544382 1104 8 -0.09254985886222755 1104 9 -0.1041853976003253 1104 10-11 -0.1507576561690911 1104 12-13 -0.13094651949199232 1104 14-15 0.026138670776816753 1104 16-17 0.039829455012103665 1104 18-19 -0.04647652575736316 1104 20-21 -0.0694597893402431 1104 22-23 -0.2232242314273165 1104 24-25 -0.018931786864698097 1104 26-27 -0.005361090751627273 1104 28-29 -0.09410354221242301 1104 30-31 -0.16291510284023047 1104 32-33 -0.020856287148646402 1104 34-35 -0.01617423796729156 1104 36-37 -0.03610271079755023 1104 38-39 -0.005985188484451953 1104 40-41 -0.03546481354648989 1104 42-43 -0.16862675639085722 1104 44-45 -0.07629845563015891 1104 46-47 -0.011398048035083264 1104 48-49 -0.296203590276761 1104 50-51 -0.21011434741787127 1104 52-53 -0.11574261420543053 1104 54-55 -0.07061556493503218 1104 56-57 -0.10697136423272013 1104 58-59 -0.1163391396879021 1104 60-61 -0.25658518686760345 1104 62-63 -0.35115889791966026 1104 64-65 -0.34464620519313627 1104 66-67 -0.23964874572829586 1104 68-69 -0.22994438351110347 1104 70-71 -0.4583990718056157 1104 72-73 -0.3303634271136744 1104 74-75 -0.25050098582173774 1104 76-77 -0.39722709601262096 1104 78-79 -0.4781546479004142 1104 80-81 -0.3768879036539552 1104 82-83 -0.24728088507307788 1104 84-85 -0.09527502130641352 1104 86-87 -0.14949917038203608 1104 88-89 -0.12616935893453274 1104 90-91 -0.10296214240456081 1104 92-93 -0.09431365975101258 1104 94-95 -0.15223388825872064 1104 96-97 -0.07918195642632142 1104 98-99 0.0591842229958246 1104 100-101 0.3514560505689879 1105 1 0.028094392138537927 1105 2 -0.06619882646788966 1105 3 -0.003323564895765685 1105 4 0.02080767882577561 1105 5 0.022358167607208657 1105 6 0.055449808891431474 1105 7 0.07812052870396258 1105 8 0.010453347595081652 1105 9 -0.1713002284874392 1105 10-11 -0.16300760798462477 1105 12-13 0.004931927157578286 1105 14-15 -0.08146250200392302 1105 16-17 0.01840897690536991 1105 18-19 -0.01858326749445638 1105 20-21 -0.0444044502091927 1105 22-23 -0.07505932957761985 1105 24-25 -0.05990931830141477 1105 26-27 0.07123644337173829 1105 28-29 -0.08144913289559241 1105 30-31 -0.2397106313216284 1105 32-33 -0.05109277067540319 1105 34-35 0.12436204055645561 1105 36-37 0.07249193809830246 1105 38-39 0.027122213233830905 1105 40-41 0.16138217010583134 1105 42-43 0.20979769912465684 1105 44-45 0.1936724000675838 1105 46-47 0.011662454134679479 1105 48-49 -0.15998639221815836 1105 50-51 -0.18192263748252202 1105 52-53 -0.18820475289492578 1105 54-55 -0.1332045601866767 1105 56-57 -0.10283859704254183 1105 58-59 -0.20435785278298368 1105 60-61 -0.12694414655035047 1105 62-63 -0.09828476743027892 1105 64-65 -0.3679601868655098 1105 66-67 -0.23256708911709012 1105 68-69 -0.24658321026311825 1105 70-71 -0.08295212436464539 1105 72-73 0.049186208728556835 1105 74-75 0.04309602849343008 1105 76-77 0.00355991695922242 1105 78-79 0.11155168967120588 1105 80-81 0.0864250267209492 1105 82-83 0.14925596582373757 1105 84-85 -0.023178500517005318 1105 86-87 3.65037027272308E-4 1105 88-89 0.2129409513734366 1105 90-91 0.1287549184013166 1105 92-93 0.29824052587308003 1105 94-95 0.2873822129461807 1105 96-97 0.3812658962985829 1105 98-99 0.416193748919067 1105 100-101 0.5628830700822363 1106 1 0.009825576392877622 1106 2 0.0967727233107496 1106 3 -0.025041677093476267 1106 4 -0.011255856302291534 1106 5 -0.030087910159195985 1106 6 -0.02304665795364258 1106 7 0.026421777948620218 1106 8 0.05810716007272276 1106 9 0.12535406494323098 1106 10-11 -0.07556294841304378 1106 12-13 -0.03726121864518461 1106 14-15 0.14812844128061187 1106 16-17 0.024804418950537865 1106 18-19 -0.020566890427282658 1106 20-21 0.010574646832324675 1106 22-23 -0.009337715055302453 1106 24-25 0.06196726175260636 1106 26-27 0.20272961753838814 1106 28-29 -0.05151658964064865 1106 30-31 0.08252049743813217 1106 32-33 0.21139350983120409 1106 34-35 0.20526512235547045 1106 36-37 0.28057194895057336 1106 38-39 0.2548984837514112 1106 40-41 0.2743093242204466 1106 42-43 0.3084097180174794 1106 44-45 0.3584980854256443 1106 46-47 0.3558774524698407 1106 48-49 0.1963455393631861 1106 50-51 0.1716173001169068 1106 52-53 0.3210782571310915 1106 54-55 0.41105360059962237 1106 56-57 0.2084403680451956 1106 58-59 0.42970595365192565 1106 60-61 0.3536050031493616 1106 62-63 0.302235648409102 1106 64-65 0.03980473809347984 1106 66-67 0.0669988199457876 1106 68-69 0.1560231851789311 1106 70-71 -0.0445998201063631 1106 72-73 0.0033760495207744157 1106 74-75 0.11130996626550527 1106 76-77 0.05938623125586062 1106 78-79 -2.483078623676249E-4 1106 80-81 0.05395329812644434 1106 82-83 0.25057427040265523 1106 84-85 0.27890254623410016 1106 86-87 0.20194786577100032 1106 88-89 0.11099247500796494 1106 90-91 0.26203378483202755 1106 92-93 0.3478045851166236 1106 94-95 0.4970931478448719 1106 96-97 0.5748618217632 1106 98-99 0.4091866332806937 1106 100-101 0.2983089601536406 1107 1 -0.19301836978574016 1107 2 -0.16025717986714128 1107 3 -0.10938560700858346 1107 4 -0.12735564921356968 1107 5 -0.17960786548874808 1107 6 -0.15012296635320155 1107 7 -0.1881495158679911 1107 8 -0.0831631286588248 1107 9 -0.11569287155317198 1107 10-11 -0.22585406528263974 1107 12-13 -0.11405278005079111 1107 14-15 -0.14636785063859037 1107 16-17 -0.1666875956649463 1107 18-19 -0.007499835966200408 1107 20-21 -0.21238116520483885 1107 22-23 -0.3285213409511556 1107 24-25 -0.26172677821227097 1107 26-27 -0.15298931102142177 1107 28-29 -0.07365800630098818 1107 30-31 -0.136140392067567 1107 32-33 -0.11100554796932016 1107 34-35 -0.009124562240813816 1107 36-37 -0.062328621356591896 1107 38-39 0.10373794662181268 1107 40-41 0.1346979137402471 1107 42-43 -0.002839346830562306 1107 44-45 0.13577944960905342 1107 46-47 -0.10887138468903146 1107 48-49 0.09833754855749532 1107 50-51 -0.017463648804820764 1107 52-53 -0.07997046835166799 1107 54-55 -0.0064287263837101705 1107 56-57 -0.057223427208022315 1107 58-59 0.09745256270961278 1107 60-61 -0.09912509844338047 1107 62-63 0.006506717789243055 1107 64-65 0.03881334149146909 1107 66-67 -0.0985278196873054 1107 68-69 0.20144243141934481 1107 70-71 -0.005402044954841756 1107 72-73 0.14358717701586698 1107 74-75 0.06440667453062687 1107 76-77 0.07702807618868945 1107 78-79 0.01659933555470161 1107 80-81 0.1679686508820275 1107 82-83 0.4330108969165174 1107 84-85 0.3240927480100737 1107 86-87 0.38348661087839275 1107 88-89 0.454236612011794 1107 90-91 0.2175984810838294 1107 92-93 0.1564447115383274 1107 94-95 0.13323433139487406 1107 96-97 0.16327637326973843 1107 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82-83 -0.6560586674230784 2316 84-85 -0.8452758931283597 2316 86-87 -0.8318581671311875 2316 88-89 -0.7095015879600659 2316 90-91 -0.67949311405361 2316 92-93 -0.7371749375351051 2316 94-95 -0.9442088021052797 2316 96-97 -1.1166186883075966 2316 98-99 -1.07324378717869 2316 100-101 -1.394995808469261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 21.0 6 54.0 7 157.0 8 370.0 9 703.0 10 1391.0 11 2655.0 12 4508.0 13 6560.0 14 6326.0 15 7497.0 16 9427.0 17 12402.0 18 10969.0 19 7122.0 20 6154.0 21 6196.0 22 6363.0 23 6805.0 24 7874.0 25 9176.0 26 11673.0 27 14268.0 28 18697.0 29 23078.0 30 28955.0 31 36286.0 32 45935.0 33 59284.0 34 79743.0 35 111492.0 36 169071.0 37 253466.0 38 251415.0 39 53905.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.64444572241571 19.685151645733757 13.577096724130733 20.093305907719795 2 15.276140621911876 23.784259208952534 38.300549533184196 22.639050635951392 3 22.214476286803368 27.370275931225546 29.746518858464434 20.668728923506656 4 12.367020962480444 17.358080235836763 40.253426884855564 30.021471916827224 5 11.661762586897485 39.57446707993742 34.148142840607825 14.615627492557268 6 26.868320537057755 38.91967991332495 18.71308653095869 15.4989130186586 7 26.606752118649073 33.024626957924646 23.161954314500985 17.206666608925296 8 24.819866601681376 31.816073780876213 20.78468950757895 22.57937010986346 9 24.115946776102316 20.21808916475267 23.65312342769586 32.01284063144915 10-11 24.580659842161335 26.04357688906806 28.671780452603123 20.70398281616748 12-13 27.859685259527232 24.098082683841056 27.79484429704641 20.2473877595853 14-15 22.373953549898896 26.718689961008117 29.297689463373462 21.609667025719524 16-17 20.028133171552508 29.17133518788124 29.525775355089756 21.274756285476496 18-19 19.664126308727898 30.621772224457512 30.149382180434962 19.564719286379624 20-21 21.903853316775507 27.222372278609303 29.270576339896355 21.60319806471884 22-23 20.295488850272157 27.973214827039655 29.61230867592996 22.118987646758228 24-25 22.569882152482894 27.794360925048284 29.501051549135415 20.134705373333407 26-27 21.996108541915856 25.916740750306506 32.26103006312792 19.826120644649716 28-29 20.089213645774027 30.011071973339064 30.257947054547973 19.641767326338933 30-31 21.498143945419237 27.514493290012272 29.596077795224495 21.391284969343992 32-33 21.445219976818024 28.0619661215542 30.2671675779872 20.22564632364058 34-35 22.65371607655389 26.599682665921232 30.55328732120429 20.19331393632058 36-37 22.488443732326953 26.416294977300925 31.60335510769508 19.491906182677038 38-39 21.345025093722356 28.29922777033356 31.068596334922223 19.28715080102187 40-41 20.773399528731808 28.83299113566973 29.69993562878083 20.69367370681764 42-43 20.340303086620025 29.11381340422784 28.272977978813547 22.272905530338587 44-45 22.042208878826656 27.18314794763264 28.414568363027858 22.360074810512845 46-47 23.019798276952248 28.812544341982694 28.499305883103315 19.668351497961744 48-49 22.607828145829117 28.415433930679125 28.81389630135897 20.16284162213279 50-51 20.115724728375238 28.972166458726463 30.66606876475644 20.246040048141865 52-53 21.464932193500687 30.140211383995887 27.57905246489991 20.815803957603517 54-55 20.48048100067794 28.919252389137483 28.110366852779368 22.489899757405205 56-57 22.521442768361403 27.497810837457042 28.41749534606092 21.563251048120627 58-59 24.305295807875872 27.360427236483297 27.694579775719745 20.639697179921082 60-61 21.921157441018476 28.673342918831406 30.092230234634727 19.31326940551539 62-63 20.895420727028338 28.552293967363298 29.495913423397212 21.05637188221116 64-65 20.321252766805113 30.955043209044785 28.191547622143265 20.532156402006834 66-67 21.74801222615311 27.930167099423546 27.46536533725696 22.856455337166377 68-69 21.079103344991175 29.163852625220088 27.627553361117357 22.12949066867138 70-71 23.023771366469656 28.819976295572943 27.21937620343281 20.936876134524592 72-73 22.26774617085063 27.130711736556883 29.861400638962543 20.74014145362994 74-75 20.788576890794015 30.838284691131335 27.96262584427671 20.410512573797934 76-77 21.658009914091263 28.18126306829747 27.565441388083013 22.595285629528263 78-79 21.927952088792583 28.846723190888934 28.2044084483975 21.020916271920985 80-81 22.859007863736185 28.197009937740503 28.090514461731313 20.853467736792002 82-83 21.811586300406653 27.654814908719118 29.536377118735818 20.997221672138412 84-85 20.83630247707296 29.144130701641803 28.857833067061833 21.161733754223402 86-87 21.179878843429584 29.30459782333945 27.465043266266488 22.05048006696448 88-89 20.67773442344268 29.193035887862095 26.964773541948567 23.16445614674666 90-91 21.785899603234906 26.972191798403333 27.733478422797734 23.508430175564026 92-93 22.396518810055387 28.7501707642878 28.007744726385415 20.845565699271393 94-95 21.466202135588322 28.6177575653301 28.406253924845338 21.509786374236242 96-97 19.25844408702277 28.919105675851792 30.091168775432664 21.731281461692774 98-99 20.948830125561233 29.94038312142652 27.267250642492847 21.843536110519402 100-101 19.748725040241162 28.764005657387838 27.561928866852938 23.925340435518063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 227.0 1 172.0 2 386.5 3 584.0 4 405.0 5 303.5 6 398.5 7 619.0 8 890.0 9 1097.0 10 1120.0 11 1109.5 12 1166.5 13 1316.0 14 1506.0 15 1600.5 16 1717.0 17 1881.0 18 2037.5 19 2245.5 20 2456.0 21 2765.0 22 3202.0 23 3715.5 24 4554.0 25 5554.0 26 6906.0 27 8640.0 28 11030.5 29 13995.0 30 16931.5 31 19935.5 32 22895.5 33 25854.5 34 28744.5 35 31599.5 36 34273.5 37 36998.0 38 39540.5 39 41192.5 40 42125.5 41 42882.5 42 43634.5 43 44914.0 44 45370.5 45 45968.0 46 48676.0 47 65027.0 48 104757.5 49 103769.0 50 66754.0 51 48189.0 52 35825.5 53 28282.0 54 23958.0 55 20581.0 56 17192.0 57 14056.0 58 11381.0 59 9280.0 60 7556.0 61 5841.5 62 4242.5 63 2981.5 64 1994.5 65 1278.0 66 784.5 67 471.5 68 288.5 69 181.5 70 109.0 71 71.5 72 43.0 73 27.5 74 21.0 75 18.0 76 14.5 77 9.5 78 6.0 79 4.5 80 5.5 81 3.0 82 1.0 83 1.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051809758974387235 2 0.003464482363028934 3 0.0431485530668149 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.9369117761692435E-5 14-15 0.0018109794170378516 16-17 0.0 18-19 0.0 20-21 0.01086587650222711 22-23 0.0 24-25 0.0 26-27 0.00551167648663694 28-29 0.012440641212694807 30-31 0.010472185324610186 32-33 0.006220320606347403 34-35 0.005984105899777249 36-37 0.02090500153145868 38-39 0.04645555895879707 40-41 0.00421249560050109 42-43 0.012834332390311733 44-45 0.012283164741648037 46-47 0.0056691529576837095 48-49 0.0018897176525612368 50-51 0.0027164691255567777 52-53 0.0015747647104676972 54-55 9.448588262806184E-4 56-57 0.010944614737750496 58-59 0.008031300023385255 60-61 0.004488079424832938 62-63 0.006338427959632482 64-65 0.07621861198663656 66-67 0.036022742751948576 68-69 0.028070180964086706 70-71 0.01779484122828498 72-73 2.75583824331847E-4 74-75 7.086441197104637E-4 76-77 0.0016141338282293898 78-79 0.001181073532850773 80-81 0.0018897176525612368 82-83 0.0017716102992761595 84-85 0.0018897176525612368 86-87 0.008188776494432027 88-89 4.330602953786167E-4 90-91 7.873823552338486E-4 92-93 4.330602953786167E-4 94-95 0.006023475017538942 96-97 0.01051155444237188 98-99 0.01358234562778389 100-101 0.014724050042872968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1270031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.08126071995261 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52881927206617 48.75906688322791 2 11.435143610370934 13.512054020288508 3 2.622056785527147 4.647432617047508 4 1.070541406799093 2.529957438664083 5 0.5930630808218621 1.7519457250707384 6 0.3577624899293275 1.2682235366000412 7 0.250726781973064 1.0369278062657714 8 0.18216549029782314 0.861005346117095 9 0.13591328807062544 0.7226935567025982 >10 0.749924780594106 7.954082522977458 >50 0.04343173866573755 1.776286345881694 >100 0.022771096209044668 2.6012107379261766 >500 0.0032337594421568477 1.3629356436583926 >1k 0.0033684994189133828 4.752801218911317 >5k 8.084398605392119E-4 3.07319260109995 >10k+ 2.694799535130707E-4 3.390183999560771 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30984 2.4396254894565566 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11603 0.9135997467778345 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7843 0.6175439812099075 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7684 0.6050246017616893 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6682 0.5261288897672576 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6157 0.4847913161174806 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5184 0.4081790129532271 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5055 0.39802178057071047 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4661 0.36699891577449684 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4625 0.364164339295655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4188 0.3297557303719358 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4172 0.32849591860356164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3307 0.26038734487583376 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3208 0.25259225955901865 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2915 0.22952195655066687 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2670 0.21023108884743757 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2364 0.1861371887772818 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2287 0.1800743446419812 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2285 0.1799168681709344 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2249 0.17708229169209255 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2066 0.16267319459131313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2048 0.16125590635189221 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2028 0.1596811416414245 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1986 0.15637413574944234 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1672 0.1316503297950995 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1670 0.13149285332405272 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1513 0.1191309503468813 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1438 0.11322558268262743 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1379 0.10858002678674772 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1338 0.10535175913028894 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1327 0.1044856385395317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.936911776169243E-5 0.0 12-13 0.0 0.0 0.0 7.873823552338486E-5 0.0 14-15 0.0 0.0 0.0 7.873823552338486E-5 0.0 16-17 0.0 0.0 0.0 7.873823552338486E-5 0.0 18-19 0.0 0.0 0.0 1.9684558880846216E-4 0.0 20-21 0.0 0.0 0.0 2.75583824331847E-4 0.0 22-23 0.0 0.0 0.0 7.086441197104638E-4 0.0 24-25 0.0 3.936911776169243E-5 0.0 0.0011417044150890804 0.0 26-27 0.0 1.574764710467697E-4 0.0 0.0014960264749443123 0.0 28-29 0.0 1.574764710467697E-4 0.0 0.0030707911854120093 0.0 30-31 0.0 1.574764710467697E-4 0.0 0.005551045604398633 0.0 32-33 0.0 1.574764710467697E-4 0.0 0.009173004438474335 0.0 34-35 0.0 1.574764710467697E-4 0.0 0.013149285332405272 0.0 36-37 0.0 1.9684558880846216E-4 0.0 0.016259445635578976 0.0 38-39 0.0 3.149529420935394E-4 0.0 0.019684558880846217 0.0 40-41 0.0 3.149529420935394E-4 0.0 0.02854261037722701 0.0 42-43 0.0 3.149529420935394E-4 0.0 0.040865144236636744 0.0 44-45 0.0 3.543220598552319E-4 0.0 0.06397481636275021 0.0 46-47 0.0 3.936911776169243E-4 0.0 0.10810759737360742 0.0 48-49 0.0 3.936911776169243E-4 0.0 0.13668957686859612 0.0 50-51 0.0 3.936911776169243E-4 0.0 0.17263358138502133 0.0 52-53 0.0 3.936911776169243E-4 0.0 0.21775059033992084 0.0 54-55 0.0 3.936911776169243E-4 0.0 0.2599542845804551 0.0 56-57 0.0 3.936911776169243E-4 0.0 0.29664630233435246 0.0 58-59 0.0 3.936911776169243E-4 0.0 0.3953053114451537 0.0 60-61 0.0 3.936911776169243E-4 0.0 0.48754715436079904 0.0 62-63 0.0 3.936911776169243E-4 0.0 0.6004184149835712 0.0 64-65 0.0 3.936911776169243E-4 0.0 0.668290774004729 0.0 66-67 0.0 3.936911776169243E-4 0.0 0.8652151010487146 0.0 68-69 0.0 3.936911776169243E-4 0.0 1.0800917457920318 0.0 70-71 0.0 3.936911776169243E-4 0.0 1.1683573078137464 0.0 72-73 0.0 3.936911776169243E-4 0.0 1.4279572703343462 0.0 74-75 0.0 3.936911776169243E-4 0.0 1.551615669223822 0.0 76-77 0.0 3.936911776169243E-4 0.0 1.633936494463521 0.0 78-79 7.873823552338486E-5 3.936911776169243E-4 0.0 1.737083582999155 0.0 80-81 7.873823552338486E-5 3.936911776169243E-4 0.0 1.8377897862335644 0.0 82-83 7.873823552338486E-5 3.936911776169243E-4 0.0 2.039438407408953 0.0 84-85 7.873823552338486E-5 3.936911776169243E-4 0.0 2.4584439277466457 0.0 86-87 7.873823552338486E-5 3.936911776169243E-4 0.0 3.1753555621870646 0.0 88-89 7.873823552338486E-5 3.936911776169243E-4 0.0 3.9784855645255908 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 335 0.0 60.973335 2 TACCTGG 2170 0.0 56.258698 2 ACCTGGG 2275 0.0 52.200527 3 TAACGCA 385 0.0 50.569134 4 GTACCTG 2540 0.0 48.28098 1 GTACACG 180 0.0 47.532433 1 ATAACGC 410 0.0 47.50248 3 GTATAAC 505 0.0 44.23811 1 CCTGGGG 1550 0.0 38.60124 4 TACACGG 230 0.0 35.110527 2 TATCACG 245 0.0 32.960903 2 TCACGCA 270 0.0 29.89837 4 TACGCAG 435 0.0 29.47385 4 GTACGAC 65 0.005842808 29.232294 3 GAGTACG 505 0.0 27.295855 1 CTGGGGG 925 0.0 26.694633 5 GTACGCA 460 0.0 25.816565 3 AGTACGC 470 0.0 25.267279 2 GGCGTCG 135 1.3456096E-5 24.62316 8 CCTGGGA 1275 0.0 24.580807 4 >>END_MODULE