FastQCFastQC Report
Wed 25 May 2016
SRR1003774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434993
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.32787616385585155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.2405586647460998No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34060.23735307419618076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22790.15881610572316382No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17250.1202096456219647No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15480.10787509067988485No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15300.1066207291603513No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15140.10550574114298816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18250.052.9660152
TATAACG2900.049.6012842
ACCTGGG20450.046.3780983
TAACGCA3700.041.942444
ATAACGC3800.039.328923
GTACCTG25000.038.6883851
GGACCGT1201.2159308E-633.533976
GTATAAC6000.031.66461
GTACACG2950.031.2254941
TACACGG2452.7284841E-1128.1816272
CCTGGGG15450.024.5531434
GTACATG64350.023.0824831
GACCGTG1752.2921755E-522.9858117
TACATGG62650.022.5006922
ACATGGG61400.021.9063363
AGTACTT57800.021.73045512-13
GTCTACG2404.8944275E-721.5895021
GAGTACT53800.021.42285312-13
ACACGGG3259.367795E-1021.2236523
CATGGGG22000.020.639354
Produced by FastQC (version 0.11.5)