FastQCFastQC Report
Wed 25 May 2016
SRR1003774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434993
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.41519366296560334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.35749303306706026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.23240531486913174No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24630.17163846792284004No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16120.11233504274933746No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14840.10341513861043224No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14800.10313639160609146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.10160328308221712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2650.060.950292
TAACGCA3100.050.54384
TACCTGG17400.045.0481952
TACACGG2200.043.18672
ACCTGGG17700.041.8691373
ATAACGC4050.041.054023
GTACCTG19950.040.978441
GTACACG2900.037.696391
GTACCGG1401.6960257E-830.5551451
GTATAAC6150.028.595421
ACACGGG3500.025.788633
CCTGGGG13400.024.8032674
ACAACGC1951.5685146E-824.3617273
TCACGCA2005.4092925E-721.3662414
TATCACG2509.482392E-920.902362
TAACCGT2509.53878E-920.8914387
GTAGCGT1150.003732573520.6437138
AGTACTT71850.020.58481412-13
GTACGCG1856.5772438E-620.5321245
GCGTGCG1404.8659727E-420.3488039
Produced by FastQC (version 0.11.5)