##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1434993 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77791947417165 31.0 31.0 34.0 30.0 34.0 2 31.979611747235005 31.0 31.0 34.0 30.0 34.0 3 32.008099691078634 33.0 31.0 34.0 30.0 34.0 4 35.812300826554555 37.0 35.0 37.0 35.0 37.0 5 35.66256351076277 37.0 35.0 37.0 33.0 37.0 6 35.71931849144909 37.0 35.0 37.0 33.0 37.0 7 35.66339487370322 37.0 35.0 37.0 33.0 37.0 8 35.65696069597552 37.0 35.0 37.0 33.0 37.0 9 37.26712534486231 39.0 37.0 39.0 33.0 39.0 10-11 37.18430124746253 39.0 37.0 39.0 33.0 39.0 12-13 37.14158187531228 39.0 37.0 39.0 33.0 39.0 14-15 38.33479501293735 40.0 38.0 41.0 33.0 41.0 16-17 38.304789640088835 40.0 38.0 41.0 33.0 41.0 18-19 38.31913744526977 40.0 38.0 41.0 33.5 41.0 20-21 38.282646326497755 40.0 38.0 41.0 33.5 41.0 22-23 38.19401314152752 40.0 37.5 41.0 33.0 41.0 24-25 38.11416850117039 40.0 37.0 41.0 33.0 41.0 26-27 37.94513562087062 40.0 37.0 41.0 33.0 41.0 28-29 37.76051660182314 40.0 37.0 41.0 33.0 41.0 30-31 37.5043533313403 39.0 36.5 41.0 32.0 41.0 32-33 37.20537556629196 39.0 36.0 41.0 31.5 41.0 34-35 37.156443968716225 39.0 36.0 41.0 31.5 41.0 36-37 36.99790521626238 39.0 36.0 41.0 31.0 41.0 38-39 36.749686583837 39.0 35.0 40.5 31.0 41.0 40-41 36.65610912387726 39.0 35.0 41.0 30.5 41.0 42-43 36.488279733768735 39.0 35.0 41.0 30.0 41.0 44-45 36.20834909996077 39.0 35.0 41.0 30.0 41.0 46-47 35.96264093274323 39.0 35.0 41.0 29.5 41.0 48-49 35.70218147405597 39.0 35.0 41.0 28.0 41.0 50-51 35.47068905562605 39.0 35.0 40.5 27.0 41.0 52-53 35.232552353913924 39.0 35.0 40.0 26.5 41.0 54-55 35.02794821995647 38.0 34.5 40.0 26.0 41.0 56-57 34.791710482211414 38.0 34.0 40.0 26.0 41.0 58-59 34.532914794706315 38.0 34.0 40.0 25.0 41.0 60-61 34.26879643315333 38.0 34.0 40.0 24.5 41.0 62-63 33.96554721869724 37.0 33.5 40.0 23.0 41.0 64-65 33.516582310854474 37.0 33.0 40.0 22.0 41.0 66-67 33.19972153174267 36.0 32.5 39.0 22.0 41.0 68-69 32.92951150284357 36.0 32.0 39.0 21.5 41.0 70-71 32.57019511593437 35.5 32.0 39.0 20.0 40.0 72-73 32.20351179413419 35.0 32.0 38.0 20.0 40.0 74-75 31.794540809606737 35.0 31.0 37.5 18.0 39.5 76-77 30.790685738536702 34.0 30.5 36.0 18.0 39.0 78-79 31.21313274698901 35.0 31.0 36.0 18.0 39.0 80-81 31.04666642973171 35.0 31.0 36.0 17.5 38.5 82-83 30.761910685278604 35.0 31.0 36.0 14.0 37.0 84-85 30.414519792082608 34.0 31.0 35.0 9.0 37.0 86-87 30.060855697553926 34.0 30.5 35.0 7.0 36.5 88-89 29.73900081742559 34.0 30.0 35.0 3.5 36.0 90-91 29.477944840149043 34.0 30.0 35.0 2.0 36.0 92-93 29.22343941747451 34.0 30.0 35.0 2.0 35.5 94-95 28.913976235424144 34.0 29.5 35.0 2.0 35.0 96-97 28.591692781776636 34.0 29.0 35.0 2.0 35.0 98-99 28.200046272002723 34.0 29.0 35.0 2.0 35.0 100-101 27.320928394772658 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09765510685752332 1101 2 0.20209343461553786 1101 3 0.1338838272770566 1101 4 0.12190157744981178 1101 5 0.19545641802879743 1101 6 0.14986270687797543 1101 7 0.1377898746484405 1101 8 0.035340912824203485 1101 9 0.10076206958519407 1101 10-11 0.2152679097323258 1101 12-13 0.19233733980458823 1101 14-15 0.315320248078244 1101 16-17 0.27573986370952497 1101 18-19 0.1828261963978477 1101 20-21 0.07961936483936682 1101 22-23 0.0316670063520732 1101 24-25 0.15568406657011025 1101 26-27 0.18738442262597488 1101 28-29 0.2600368400584685 1101 30-31 0.10360376462753607 1101 32-33 0.17916302455277133 1101 34-35 0.4377650063332439 1101 36-37 0.2277298174165523 1101 38-39 0.194143081572129 1101 40-41 0.4336162608227312 1101 42-43 0.4873222758323763 1101 44-45 0.3796263479205777 1101 46-47 0.07627793115160131 1101 48-49 0.2028926417682655 1101 50-51 0.3154841919667106 1101 52-53 0.3256256640966342 1101 54-55 0.25908442394994324 1101 56-57 0.11326511247391124 1101 58-59 0.38439581543544676 1101 60-61 0.22142952514199266 1101 62-63 0.21136124294692138 1101 64-65 0.17028070118315952 1101 66-67 0.3536280076248488 1101 68-69 0.48247755422810457 1101 70-71 0.2642082334273894 1101 72-73 0.25240409608480974 1101 74-75 0.3351536669930759 1101 76-77 0.3834922378332841 1101 78-79 0.30910721786801787 1101 80-81 0.3082045917929932 1101 82-83 0.3608366832775154 1101 84-85 0.3603468773904517 1101 86-87 0.2736950876570532 1101 88-89 0.3138741588397309 1101 90-91 0.33719292025081415 1101 92-93 0.3859712864288447 1101 94-95 0.23040696272531846 1101 96-97 0.1399745075938199 1101 98-99 0.01835399051707398 1101 100-101 0.11716294159463558 1102 1 0.08814349171210623 1102 2 0.08403431909525594 1102 3 0.11494577445759901 1102 4 0.04547854451949718 1102 5 0.09855193219848246 1102 6 0.03338271102301604 1102 7 0.11193596068475387 1102 8 0.1948610457167561 1102 9 0.12023081337842711 1102 10-11 0.07694087377487335 1102 12-13 0.06097267895615488 1102 14-15 0.0680863570221959 1102 16-17 0.08719325043643522 1102 18-19 0.0635893158873202 1102 20-21 0.007357859796634614 1102 22-23 0.0736971819432739 1102 24-25 0.07797610989522497 1102 26-27 0.02948128785951809 1102 28-29 0.05718669195957915 1102 30-31 0.11550152654041312 1102 32-33 0.07475703339709128 1102 34-35 -0.02014044431838613 1102 36-37 -0.04410289559974245 1102 38-39 -0.13039996288265598 1102 40-41 -0.12442708312703132 1102 42-43 0.09188017335406329 1102 44-45 0.24456019603461243 1102 46-47 -0.049634390221036995 1102 48-49 -0.058539587973825746 1102 50-51 -0.04691788338041647 1102 52-53 -0.03184259453827565 1102 54-55 -0.04641576406852721 1102 56-57 0.04811357115420378 1102 58-59 -0.032254103587078475 1102 60-61 -0.17784026448927648 1102 62-63 -0.35570424007999435 1102 64-65 -0.2623048629232869 1102 66-67 -0.15217446228630394 1102 68-69 -0.019815760871232158 1102 70-71 -0.07570306424550921 1102 72-73 -0.05132856127173113 1102 74-75 -0.06193977936591821 1102 76-77 -0.2094793647043005 1102 78-79 0.03909227844608054 1102 80-81 0.04598947510770657 1102 82-83 -0.0935058369236792 1102 84-85 -0.2641329316236032 1102 86-87 -0.3137576427715594 1102 88-89 -0.4439880750395524 1102 90-91 -0.23909429975278584 1102 92-93 -0.2726654736556604 1102 94-95 -0.29513744692415145 1102 96-97 -0.24202878802852723 1102 98-99 -0.29401711477248327 1102 100-101 -0.07441434106346634 1103 1 -0.11250612298874785 1103 2 -0.08204339270164951 1103 3 -0.05833718265035159 1103 4 -0.09353916949544328 1103 5 -0.08871219179626166 1103 6 0.04328992332570181 1103 7 0.05688086273845272 1103 8 -0.03149086195931261 1103 9 0.01995725602380105 1103 10-11 -0.03655938331527153 1103 12-13 -0.08370017803787277 1103 14-15 -0.013302837126232703 1103 16-17 0.005997293977088702 1103 18-19 -0.18163267220923984 1103 20-21 -0.1302626617098852 1103 22-23 -0.011020321358572005 1103 24-25 -0.1610324463335857 1103 26-27 -0.2165153313210908 1103 28-29 -0.18272588058857053 1103 30-31 -0.060661512298679554 1103 32-33 -0.049747269922228554 1103 34-35 0.05888914961361991 1103 36-37 0.07815690995273172 1103 38-39 -0.0348136797339933 1103 40-41 -0.12988478759771738 1103 42-43 0.16271102820399364 1103 44-45 0.3501116627926919 1103 46-47 0.31169740393209366 1103 48-49 0.22304637255191295 1103 50-51 0.27952610625531804 1103 52-53 0.13007714628797373 1103 54-55 0.2976607446137223 1103 56-57 0.24502613698104625 1103 58-59 0.03043724373330292 1103 60-61 0.04939885622882656 1103 62-63 0.1561345089496129 1103 64-65 0.190277128668356 1103 66-67 0.3128810221353717 1103 68-69 0.192215553716359 1103 70-71 0.13366661121599321 1103 72-73 0.3104934684969436 1103 74-75 0.274573994280491 1103 76-77 0.19623030490966187 1103 78-79 0.4734708596445536 1103 80-81 0.4233234503262331 1103 82-83 0.40711000318977497 1103 84-85 0.28927334473653943 1103 86-87 0.1453541023832159 1103 88-89 -0.22902920946240712 1103 90-91 0.012349941932114916 1103 92-93 0.16682928540214803 1103 94-95 0.1656000153104813 1103 96-97 0.22369546683014008 1103 98-99 0.19020502791422444 1103 100-101 0.2996862635184492 1104 1 0.05628335927502803 1104 2 -0.017541576677782444 1104 3 -0.0760546338796253 1104 4 -0.02982035922460824 1104 5 -0.02139109716337373 1104 6 -0.041015527676457 1104 7 -0.10524940743974298 1104 8 -0.11304800855813824 1104 9 0.06729842490943838 1104 10-11 0.018768902149396638 1104 12-13 -0.016608490353682726 1104 14-15 0.10583766982190923 1104 16-17 0.046511202174009725 1104 18-19 0.006565736869596606 1104 20-21 -0.14312959835326922 1104 22-23 -0.09840445024406819 1104 24-25 0.07477843625584768 1104 26-27 0.030005558906076146 1104 28-29 0.019230763787611238 1104 30-31 -0.1995891698934713 1104 32-33 0.07414095348809013 1104 34-35 0.1339970304062632 1104 36-37 0.05876904645031544 1104 38-39 0.16511333130868167 1104 40-41 0.12129475791094535 1104 42-43 0.0926526187222052 1104 44-45 0.31297566888822814 1104 46-47 0.18679241778809086 1104 48-49 0.07184887743768797 1104 50-51 0.18024784524241255 1104 52-53 0.2421092431132763 1104 54-55 0.23696223021832452 1104 56-57 0.11996729707504272 1104 58-59 -0.04666318064666086 1104 60-61 0.20188945558080462 1104 62-63 0.12868534937579312 1104 64-65 0.23487274053926654 1104 66-67 0.24673191769248604 1104 68-69 0.09378486208875358 1104 70-71 0.17641323055431712 1104 72-73 0.09263264937219162 1104 74-75 0.10166182646266719 1104 76-77 -0.17499340944984 1104 78-79 0.0934793124435025 1104 80-81 0.2189033141656509 1104 82-83 0.31875169609400444 1104 84-85 0.07320611247413922 1104 86-87 0.06746380377870409 1104 88-89 -0.05035210455062611 1104 90-91 0.03721106143802544 1104 92-93 0.0911904717611769 1104 94-95 0.1033574685178742 1104 96-97 0.07495482397783704 1104 98-99 0.007432482385414829 1104 100-101 0.09167414570310228 1105 1 -0.0708912590572659 1105 2 -0.035346104862629346 1105 3 -0.02686989895555314 1105 4 0.02432932062750126 1105 5 -0.0680716797550005 1105 6 0.011051354462601637 1105 7 -0.023632099457067568 1105 8 0.03299778748581872 1105 9 -0.07537704429392278 1105 10-11 -0.03667162257483625 1105 12-13 0.016989949511973634 1105 14-15 -0.012442336136231802 1105 16-17 -0.1268162852465622 1105 18-19 -0.2760604246612388 1105 20-21 -0.16193140048595467 1105 22-23 -0.10982540493365178 1105 24-25 -0.10808717304537652 1105 26-27 -0.2176981604411381 1105 28-29 -0.187284521284802 1105 30-31 -0.09830771112909531 1105 32-33 0.04500856651926455 1105 34-35 -0.08401445125205953 1105 36-37 -0.20942597442356714 1105 38-39 -0.1605504297455056 1105 40-41 0.0454789827204678 1105 42-43 0.13240355019137695 1105 44-45 0.07352534792416066 1105 46-47 0.09027236824577045 1105 48-49 0.07016498930376969 1105 50-51 0.13544831005879132 1105 52-53 0.14062475875746827 1105 54-55 0.28296027954028347 1105 56-57 0.10848563875384087 1105 58-59 0.04048121849962172 1105 60-61 0.15129195840684417 1105 62-63 6.39965504390716E-4 1105 64-65 -0.15810777716578883 1105 66-67 -0.09866868546696139 1105 68-69 -0.1567938628697263 1105 70-71 -0.20608162749100956 1105 72-73 -0.23261791889541783 1105 74-75 -0.07253311075243474 1105 76-77 -0.15820400922212485 1105 78-79 -0.23192122950219485 1105 80-81 -0.21464737897800035 1105 82-83 -0.1924001731697551 1105 84-85 -0.240070026815701 1105 86-87 -0.24738833441744745 1105 88-89 -0.2605108828058711 1105 90-91 -0.10037856568864356 1105 92-93 -0.11399621996231346 1105 94-95 -0.24462584461492298 1105 96-97 -0.15576820218491605 1105 98-99 0.0883925077687131 1105 100-101 0.24126141201962525 1106 1 -0.05374805748015987 1106 2 0.06643463707574782 1106 3 -0.00995420970548011 1106 4 -0.04841316614724178 1106 5 -0.12643893310800536 1106 6 0.024746414667681904 1106 7 0.05360744915847704 1106 8 0.0364323372123323 1106 9 0.04125168395353285 1106 10-11 -0.006977718250567477 1106 12-13 -0.055820200019759625 1106 14-15 0.028632503009603738 1106 16-17 -0.0424254627983629 1106 18-19 -0.06343597246364396 1106 20-21 0.03347273231978676 1106 22-23 0.1192215961584111 1106 24-25 -0.08836303617396624 1106 26-27 0.08690745728142701 1106 28-29 -0.04647448284999456 1106 30-31 0.052987099935215554 1106 32-33 -0.08455202149077223 1106 34-35 -0.02361111030740659 1106 36-37 0.07563249328978117 1106 38-39 0.16379409837620074 1106 40-41 0.22283281934652166 1106 42-43 0.4092975755403856 1106 44-45 0.23601110624342425 1106 46-47 0.209431210852145 1106 48-49 0.03330031009024026 1106 50-51 0.07332651236866639 1106 52-53 0.2404385532139628 1106 54-55 0.2935428529233519 1106 56-57 0.23413862867948154 1106 58-59 0.35638828946454737 1106 60-61 0.2372777309087013 1106 62-63 0.14287535389452444 1106 64-65 0.1729663883071737 1106 66-67 0.029567898987977514 1106 68-69 -0.11430135600298996 1106 70-71 0.14280734412964335 1106 72-73 0.1891716162466679 1106 74-75 0.09637558648243427 1106 76-77 4.0872106762535054E-4 1106 78-79 0.20286310063059787 1106 80-81 0.16386842515102273 1106 82-83 0.14152853333106563 1106 84-85 0.0681678104343888 1106 86-87 0.12077299959664956 1106 88-89 0.0831600788207787 1106 90-91 0.05200328154598921 1106 92-93 0.027471831397559043 1106 94-95 0.1379937500467534 1106 96-97 0.04643914014017625 1106 98-99 0.016650188821255796 1106 100-101 0.3113561871547148 1107 1 -0.23308511002130672 1107 2 -0.15402047229937565 1107 3 -0.031031499326115153 1107 4 -0.04115617529051718 1107 5 -0.052843841181669404 1107 6 0.002691965892161363 1107 7 -0.027511434759013298 1107 8 -0.02205113845940332 1107 9 -0.01290961334030527 1107 10-11 -0.08508008184181648 1107 12-13 -0.07133368548310415 1107 14-15 -0.05591477548542656 1107 16-17 -0.09043666044197352 1107 18-19 0.0012432925525871497 1107 20-21 -0.17122443505257223 1107 22-23 0.10298074410230385 1107 24-25 -0.08070384260707186 1107 26-27 -1.2607428514854746E-4 1107 28-29 -0.056878059585997676 1107 30-31 0.0651635702095632 1107 32-33 0.04699527834835493 1107 34-35 0.032499502774925304 1107 36-37 -0.15354834718716148 1107 38-39 0.06954626413440934 1107 40-41 -0.05744644140089861 1107 42-43 -0.3743527175406598 1107 44-45 -0.16782576087093304 1107 46-47 -0.3857604324027264 1107 48-49 -0.38527331768482753 1107 50-51 -0.6352402719573007 1107 52-53 -0.45584837539805534 1107 54-55 -0.45045845707021925 1107 56-57 -0.4637814563767222 1107 58-59 -0.4794799881473608 1107 60-61 -0.6756180352241046 1107 62-63 -0.5958729752633687 1107 64-65 -0.44559836833016675 1107 66-67 -0.5080760735741947 1107 68-69 -0.4079410170588247 1107 70-71 -0.35936241609618236 1107 72-73 -0.4143025347254188 1107 74-75 -0.2655951801172627 1107 76-77 -0.1678227342525247 1107 78-79 -5.00061069562463E-4 1107 80-81 -0.09069320724503882 1107 82-83 -0.10671824971425181 1107 84-85 -0.19404634550020106 1107 86-87 -0.26309747112059156 1107 88-89 -0.3953937789902291 1107 90-91 -0.3460866891325658 1107 92-93 -0.29054578281333576 1107 94-95 -0.04552040775544697 1107 96-97 -0.02647994441177204 1107 98-99 0.13033782072106348 1107 100-101 0.13066778686763314 1108 1 0.04131546256607166 1108 2 0.045343319501494506 1108 3 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-0.3216492428135638 2316 84-85 -0.5051444553111821 2316 86-87 -0.7033683982132999 2316 88-89 -0.38636707631996003 2316 90-91 -0.516594939957649 2316 92-93 -0.785216049840038 2316 94-95 -0.8677853342480972 2316 96-97 -1.1529890953268627 2316 98-99 -1.0898352965906675 2316 100-101 -1.401597439655017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 10.0 5 24.0 6 88.0 7 188.0 8 452.0 9 901.0 10 1673.0 11 2954.0 12 4973.0 13 7063.0 14 7855.0 15 9731.0 16 12142.0 17 15775.0 18 13655.0 19 8776.0 20 7296.0 21 7148.0 22 7311.0 23 7965.0 24 9348.0 25 11109.0 26 14072.0 27 17702.0 28 22339.0 29 28460.0 30 34791.0 31 43713.0 32 54843.0 33 70848.0 34 94321.0 35 127858.0 36 186153.0 37 274826.0 38 271211.0 39 57377.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.140199582929824 21.11830862774709 14.504030214231282 21.237461575091803 2 16.0279622227054 21.36736411937536 40.47853005290828 22.126143605010956 3 18.403269895337417 27.675037767904637 30.646693887908622 23.274998448849324 4 12.87030668442285 17.891933967622144 38.39342770313166 30.844331644823352 5 12.068003119178979 38.24506461007127 35.253412385983765 14.433519884765989 6 28.513031074019175 38.88834300933872 18.5784878393135 14.0201380773286 7 26.53992040379291 33.20956966340602 23.04108800530734 17.20942192749372 8 25.342562646647053 35.67703814583068 20.57884602921408 18.401553178308188 9 25.413852193007212 17.34266299556862 22.727358251921785 34.51612655950238 10-11 23.85743345089488 27.387276453613364 28.736446797998315 20.01884329749344 12-13 24.93217736950633 25.097544029831504 29.242964948261076 20.72731365240109 14-15 21.89815153574104 26.83698322270423 27.820867959372116 23.443997282182615 16-17 20.272363697941383 29.951748893548608 28.74386843698889 21.032018971521115 18-19 20.112084170445428 28.380068752948624 31.853326113785922 19.654520962820026 20-21 21.10044715662469 27.738729785258887 31.50913966953899 19.651683388577435 22-23 20.637069309745762 27.63236475718 31.529526624868552 20.201039308205683 24-25 20.675884830100216 27.833515564187422 31.556913518045036 19.933686087667326 26-27 20.432131933105165 27.706321446168364 31.835816766080637 20.025729854645835 28-29 20.486124541971364 27.78147743528358 31.982499516489032 19.749898506256024 30-31 20.757918946231314 28.07241175035718 31.54991810990696 19.61975119350455 32-33 20.497357141189536 28.097897804348655 31.51889202382458 19.88585303063723 34-35 20.739066091808834 27.596869619636973 31.689299399855948 19.974764888698246 36-37 21.239693039011367 27.766238473280314 31.23080622564839 19.76326226205993 38-39 21.008598157945922 28.091603532404662 31.3098648402756 19.589933469373815 40-41 20.60929507941381 28.018311815881088 31.25943214275634 20.112960961948765 42-43 20.543279519027013 28.21569490428037 30.937598334032018 20.3034272426606 44-45 20.794994607459653 28.38371888399679 30.471738384259012 20.349548124284553 46-47 20.94345171516719 28.5222027788057 30.145728058383213 20.3886174476439 48-49 21.269233328908175 28.413914792332832 30.048436333277934 20.26841554548106 50-51 21.125716221056727 28.72713702523133 29.578727520310643 20.568419233401304 52-53 20.941175896692744 29.00302199017897 29.435710495222413 20.620091617905874 54-55 20.970913839794058 29.244977971787723 29.306407484992803 20.47770070342542 56-57 21.243907895534655 28.937957931461778 29.089437285735293 20.728696887268278 58-59 21.21376319836196 28.525571221934587 29.515511527234423 20.74515405246903 60-61 21.215696550455288 28.80172609647686 29.73470883969353 20.24786851337432 62-63 21.06276789506824 28.908049161151528 29.43149534797742 20.597687595802814 64-65 20.91314304522341 28.970785949585643 29.44594835833968 20.67012264685126 66-67 20.904880508984395 28.61116745890503 29.35405212670516 21.129899905405413 68-69 21.178138116034308 29.17304008132878 29.106509125266044 20.542312677370866 70-71 21.238843377255893 28.936721406079464 29.066774556418395 20.75766066024625 72-73 21.086767155171813 28.723405738019082 29.20076098091276 20.989066125896347 74-75 21.210600770737077 29.05556833148663 29.154663098697554 20.57916779907874 76-77 21.15883481668269 28.963971387844257 28.88184506522544 20.99534873024761 78-79 21.044575378836715 29.045216500520908 28.894413530455022 21.015794590187355 80-81 21.09653640254721 29.15329251516744 28.729315071547934 21.02085601073742 82-83 20.96890413956412 28.873219315642984 29.18994802722264 20.967928517570257 84-85 20.836038070150682 28.896275738554305 28.887460300038505 21.38022589125651 86-87 21.022809831423775 28.931700128821657 28.5008078794115 21.544682160343072 88-89 21.06127457897834 28.853062892707033 28.884874927830584 21.200787600484045 90-91 20.931238161872415 28.247009370152377 29.2118995220846 21.60985294589061 92-93 20.539607111833995 28.725700589238173 29.05782868643198 21.67686361249585 94-95 20.302761052572894 28.769094608908958 29.26830713051235 21.659837208005797 96-97 20.44937649899315 28.824259724312444 29.27567814685333 21.450685629841075 98-99 20.30035835599483 28.99327890856835 28.92606102505704 21.780301710379778 100-101 19.899071242168734 29.38955942806108 28.501676280173825 22.209693049596357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 314.0 1 245.5 2 448.5 3 601.0 4 394.0 5 342.0 6 503.0 7 861.0 8 1239.5 9 1535.5 10 1556.5 11 1467.0 12 1581.0 13 1783.5 14 2030.0 15 2257.0 16 2539.5 17 2860.5 18 3052.5 19 3264.0 20 3647.0 21 4098.0 22 4792.5 23 5796.5 24 6999.0 25 8389.5 26 10204.0 27 12734.5 28 16113.5 29 19862.0 30 23280.5 31 26760.5 32 30404.0 33 33750.0 34 37064.0 35 40371.0 36 43297.0 37 46800.0 38 50510.5 39 52522.5 40 53765.5 41 54908.5 42 56010.0 43 58001.0 44 58785.5 45 59011.5 46 59880.0 47 60428.0 48 64337.0 49 63101.5 50 57151.5 51 51312.0 52 42612.0 53 35986.0 54 30809.0 55 26329.0 56 22144.0 57 17676.5 58 14055.0 59 11473.0 60 9219.5 61 7069.5 62 5147.0 63 3573.5 64 2359.5 65 1491.5 66 905.5 67 544.0 68 337.5 69 182.5 70 96.5 71 63.0 72 37.5 73 22.0 74 14.0 75 10.5 76 8.0 77 4.5 78 4.5 79 4.5 80 3.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04892009926180824 2 0.003972144811856225 3 0.04000019512290304 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012543615195335447 16-17 0.0 18-19 0.0 20-21 0.010383325911694344 22-23 0.0 24-25 0.0 26-27 0.005853687091156542 28-29 0.01223002481545206 30-31 0.009965205405183161 32-33 0.006167277471039928 34-35 0.006097590719954731 36-37 0.01972135055711073 38-39 0.04292703866848131 40-41 0.005052289453676777 42-43 0.010557542789407336 44-45 0.009790988527470169 46-47 0.004181205065111816 48-49 0.0011498313929057494 50-51 0.002160289283641105 52-53 8.013976374797646E-4 54-55 0.001045301266277954 56-57 0.010034892156268358 58-59 0.007561012492743868 60-61 0.005505253335730558 62-63 0.005540096711273155 64-65 0.06669022078853347 66-67 0.03135903798833862 68-69 0.02247397722497601 70-71 0.013588916461613403 72-73 2.090602532555908E-4 74-75 2.090602532555908E-4 76-77 5.923373842241739E-4 78-79 5.574940086815754E-4 80-81 7.665542619371663E-4 82-83 7.665542619371663E-4 84-85 7.317108863945678E-4 86-87 0.004425108693910005 88-89 1.045301266277954E-4 90-91 7.665542619371663E-4 92-93 3.135903798833862E-4 94-95 0.002299662785811499 96-97 0.0049826027025915804 98-99 0.007456482366116072 100-101 0.008327566754681033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1434993.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.033999134864295 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02006835820008 51.70052917932294 2 11.735719861823561 14.794987112343918 3 2.672947579306619 5.0545972620465305 4 1.0601343582903866 2.672980328932646 5 0.5827593791984194 1.8366827102113608 6 0.346284820794216 1.309663025661555 7 0.25937965447427463 1.1444815841012976 8 0.18414381923534145 0.9285857073896895 9 0.13891111536696268 0.7880510813277743 >10 0.8932237161682275 10.607498580111677 >50 0.0714945290634385 3.113608956701734 >100 0.03270072650785915 3.802669211325669 >500 0.0012276448638106807 0.5407690263029197 >1k 7.81228549697706E-4 0.9248719121639761 >5k 2.232081570564874E-4 0.7800243220562995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5958 0.41519366296560334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5130 0.35749303306706026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3335 0.23240531486913174 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2463 0.17163846792284004 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1612 0.11233504274933746 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1484 0.10341513861043224 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1480 0.10313639160609146 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.10160328308221712 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.484337554259847E-5 0.0 12-13 0.0 0.0 0.0 6.968675108519694E-5 0.0 14-15 0.0 0.0 0.0 1.0453012662779541E-4 0.0 16-17 0.0 6.968675108519694E-5 0.0 2.7874700434078775E-4 0.0 18-19 0.0 6.968675108519694E-5 0.0 3.832771309685831E-4 0.0 20-21 0.0 6.968675108519694E-5 0.0 4.878072575963785E-4 0.0 22-23 0.0 6.968675108519694E-5 0.0 6.620241353093709E-4 0.0 24-25 6.968675108519694E-5 6.968675108519694E-5 0.0 0.0012543615195335447 0.0 26-27 6.968675108519694E-5 6.968675108519694E-5 0.0 0.0018466989037577188 0.0 28-29 6.968675108519694E-5 6.968675108519694E-5 0.0 0.003797927934143233 0.0 30-31 6.968675108519694E-5 6.968675108519694E-5 0.0 0.009790988527470169 0.0 32-33 6.968675108519694E-5 6.968675108519694E-5 0.0 0.02195132659183703 0.0 34-35 6.968675108519694E-5 6.968675108519694E-5 0.0 0.0317771584948498 0.0 36-37 6.968675108519694E-5 6.968675108519694E-5 0.0 0.038641303476741704 0.0 38-39 6.968675108519694E-5 6.968675108519694E-5 0.0 0.04606294246731517 0.0 40-41 6.968675108519694E-5 6.968675108519694E-5 0.0 0.061115280701717706 0.0 42-43 6.968675108519694E-5 6.968675108519694E-5 0.0 0.08282270366475655 0.0 44-45 6.968675108519694E-5 1.0453012662779541E-4 0.0 0.10198656021318571 0.0 46-47 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.12139432039041305 0.0 48-49 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.1365511887514434 0.0 50-51 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.1660983712115669 0.0 52-53 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.2724055099920348 0.0 54-55 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.38982768557059166 0.0 56-57 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.4616747259394297 0.0 58-59 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.6124071685367106 0.0 60-61 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.7832442388220708 0.0 62-63 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.8526870862784697 0.0 64-65 6.968675108519694E-5 1.3937350217039387E-4 0.0 0.9201438613289403 0.0 66-67 6.968675108519694E-5 1.3937350217039387E-4 0.0 1.2160338064366865 0.0 68-69 6.968675108519694E-5 1.3937350217039387E-4 0.0 1.433456469822501 0.0 70-71 6.968675108519694E-5 1.3937350217039387E-4 0.0 1.4962442325502634 0.0 72-73 6.968675108519694E-5 1.3937350217039387E-4 0.0 1.6277779752235726 0.0 74-75 6.968675108519694E-5 1.3937350217039387E-4 0.0 1.8499044943076377 0.0 76-77 6.968675108519694E-5 1.3937350217039387E-4 0.0 2.0475012770097134 0.0 78-79 6.968675108519694E-5 1.3937350217039387E-4 0.0 2.2062476959817925 0.0 80-81 6.968675108519694E-5 1.3937350217039387E-4 0.0 2.4822072302791724 0.0 82-83 6.968675108519694E-5 1.3937350217039387E-4 0.0 2.8212332743086552 0.0 84-85 6.968675108519694E-5 2.7874700434078775E-4 0.0 3.1732558974155274 0.0 86-87 6.968675108519694E-5 3.4843375542598467E-4 0.0 3.8068826816576804 0.0 88-89 6.968675108519694E-5 5.22650633138977E-4 0.0 4.642252610291479 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 265 0.0 60.95029 2 TAACGCA 310 0.0 50.5438 4 TACCTGG 1740 0.0 45.048195 2 TACACGG 220 0.0 43.1867 2 ACCTGGG 1770 0.0 41.869137 3 ATAACGC 405 0.0 41.05402 3 GTACCTG 1995 0.0 40.97844 1 GTACACG 290 0.0 37.69639 1 GTACCGG 140 1.6960257E-8 30.555145 1 GTATAAC 615 0.0 28.59542 1 ACACGGG 350 0.0 25.78863 3 CCTGGGG 1340 0.0 24.803267 4 ACAACGC 195 1.5685146E-8 24.361727 3 TCACGCA 200 5.4092925E-7 21.366241 4 TATCACG 250 9.482392E-9 20.90236 2 TAACCGT 250 9.53878E-9 20.891438 7 GTAGCGT 115 0.0037325735 20.643713 8 AGTACTT 7185 0.0 20.584814 12-13 GTACGCG 185 6.5772438E-6 20.532124 5 GCGTGCG 140 4.8659727E-4 20.348803 9 >>END_MODULE