FastQCFastQC Report
Wed 25 May 2016
SRR1003773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724373
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46970.2723888625024864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.25069981958659754No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40680.2359118357803097No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34250.19862291975112112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.17849966335589806No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30350.1760060033415044No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27990.16231986930901843No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26520.15379503158539365No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23290.13506358543076238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19410.11256265320786164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.10803926992593829No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18270.10595155456505059No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18170.10537163363147069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12950.027.1045842
TAACGCA3001.2732926E-1124.8901824
GAGTACT54250.023.14894912-13
AGTACTT56000.022.88739812-13
GTACATG70650.022.6539921
GTACTTT55300.022.60051314-15
ACCTGGG15350.022.4503673
TACATGG67450.022.0953272
TATAACG2905.7607394E-921.826162
GGGTCGT1955.2942974E-520.6227136
ACATGGG68050.020.5940723
GTACTAG5600.019.5333921
GTACCTG19400.018.6960951
CAGTCCG1550.00697836918.5265039
GTCGTAA2501.6765085E-518.4230731
TATGCGA2201.337771E-418.278155
TACTTTT70900.018.19509114-15
GTATAAC5700.018.1806641
CATGGGG31950.017.7979644
ACCGATC1950.001256084417.672478
Produced by FastQC (version 0.11.5)