FastQCFastQC Report
Wed 25 May 2016
SRR1003773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724373
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86310.5005297577728253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52990.3073001027039973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43920.254701274028299No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37920.219906018013504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.16069609069499466No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25910.15025751389055617No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA22360.12967032074846915No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21780.12630677933370565No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18890.10954706435324607No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18170.10537163363147069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG1955.3842086E-1026.7848789
TCACGCA1501.0643507E-625.323154
CGTGACG1351.8189894E-1224.66478564-65
ACGGGCG3300.023.0210465
GGCGTCG3400.022.3439588
TACGTGC2551.1983502E-820.4819588
GAGTACT61200.020.13341912-13
GTACTTT61750.019.88152114-15
AGTACTT61000.019.77131312-13
GAGTACG2451.7438288E-719.4019011
GTACATG76800.018.5682281
GCGTCGG4350.018.5562939
GCATCGT5400.018.4647968
CGGGCGT4151.8189894E-1218.3058916
TACATGG76100.018.1017782
TATACGG5550.017.9735682
CAATGCG2400.017.8130918-19
GTATACG6200.017.6338251
CTAACGC1650.001463890517.2732983
GAGCGTA1650.001468162417.2657857
Produced by FastQC (version 0.11.5)