##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1724373 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.885616395060698 31.0 31.0 34.0 30.0 34.0 2 32.08198980151046 33.0 31.0 34.0 30.0 34.0 3 32.14388592259331 33.0 31.0 34.0 30.0 34.0 4 35.89069940204352 37.0 35.0 37.0 35.0 37.0 5 35.75207104263405 37.0 35.0 37.0 35.0 37.0 6 35.805353018169505 37.0 35.0 37.0 35.0 37.0 7 35.74688480972504 37.0 35.0 37.0 33.0 37.0 8 35.73924377150419 37.0 35.0 37.0 33.0 37.0 9 37.38018688532006 39.0 37.0 39.0 34.0 39.0 10-11 37.302611441956 39.0 37.0 39.0 34.0 39.0 12-13 37.26247859366854 39.0 37.0 39.0 33.5 39.0 14-15 38.48692191306637 40.0 38.0 41.0 34.0 41.0 16-17 38.461696802257975 40.0 38.0 41.0 34.0 41.0 18-19 38.461182992310825 40.0 38.0 41.0 34.0 41.0 20-21 38.41755959992415 40.0 38.0 41.0 34.0 41.0 22-23 38.348884203127746 40.0 38.0 41.0 34.0 41.0 24-25 38.247122577307806 40.0 38.0 41.0 33.5 41.0 26-27 38.09598677316335 40.0 37.0 41.0 33.0 41.0 28-29 37.93868959906006 40.0 37.0 41.0 33.0 41.0 30-31 37.73464731818464 40.0 37.0 41.0 33.0 41.0 32-33 37.48857613752941 39.0 36.0 41.0 32.0 41.0 34-35 37.4929417823174 39.0 36.0 41.0 32.0 41.0 36-37 37.36404971546179 39.0 36.0 41.0 32.0 41.0 38-39 37.13992187305183 39.0 36.0 41.0 31.0 41.0 40-41 37.14834725433535 39.0 36.0 41.0 31.0 41.0 42-43 37.0188758464671 39.0 36.0 41.0 31.0 41.0 44-45 36.72767231915601 39.0 36.0 41.0 30.0 41.0 46-47 36.56370141494909 39.0 36.0 41.0 30.0 41.0 48-49 36.34494885967247 39.0 35.5 41.0 30.0 41.0 50-51 36.13164205192264 39.0 35.0 41.0 29.5 41.0 52-53 35.89955885414582 39.0 35.0 41.0 28.5 41.0 54-55 35.721550963741606 39.0 35.0 40.0 28.0 41.0 56-57 35.48297352139009 38.5 35.0 40.0 28.0 41.0 58-59 35.220221784961836 38.0 34.0 40.0 27.0 41.0 60-61 34.92925573527305 38.0 34.0 40.0 26.5 41.0 62-63 34.642884399141025 37.5 34.0 40.0 26.0 41.0 64-65 34.17547711545008 37.0 33.5 40.0 26.0 41.0 66-67 33.85410552125323 36.5 33.0 39.0 25.0 41.0 68-69 33.596223960825185 36.0 33.0 39.0 25.0 41.0 70-71 33.240639061270386 35.5 33.0 39.0 24.5 40.0 72-73 32.87603349159376 35.0 32.0 38.0 24.0 40.0 74-75 32.47577931224857 35.0 32.0 37.5 23.0 39.5 76-77 31.462803871320183 34.0 30.5 36.0 22.0 39.0 78-79 31.8807583974001 35.0 32.0 36.0 23.0 39.0 80-81 31.711284913414907 35.0 32.0 36.0 22.0 38.5 82-83 31.437394055694448 35.0 32.0 36.0 21.5 37.0 84-85 31.10824775150156 35.0 31.0 35.0 20.0 37.0 86-87 30.772124998477707 34.0 31.0 35.0 19.5 36.5 88-89 30.47576017485776 34.0 31.0 35.0 18.0 36.0 90-91 30.223337700137964 34.0 31.0 35.0 17.5 36.0 92-93 29.993958963634896 34.0 31.0 35.0 11.5 36.0 94-95 29.691634582541017 34.0 30.5 35.0 4.5 35.0 96-97 29.387295266163413 34.0 30.0 35.0 2.0 35.0 98-99 29.022147760374352 34.0 30.0 35.0 2.0 35.0 100-101 28.167956120862485 33.5 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.06389742502523887 1101 2 0.1389966284693287 1101 3 0.09536304781997984 1101 4 0.10535140606707927 1101 5 0.03223978866859056 1101 6 0.13972197608959647 1101 7 0.09237581920544358 1101 8 0.0772624514524054 1101 9 0.09682757633199657 1101 10-11 0.2127274405823556 1101 12-13 0.18360209587279286 1101 14-15 0.298970070288469 1101 16-17 0.24677155099911374 1101 18-19 0.1503743547184655 1101 20-21 0.0704145893252317 1101 22-23 0.05042760515505762 1101 24-25 0.14307698043735684 1101 26-27 0.21947365124901097 1101 28-29 0.14933270626506356 1101 30-31 0.174394125291208 1101 32-33 0.13282705961768926 1101 34-35 0.14067577317245394 1101 36-37 0.19352216450270276 1101 38-39 0.1824765576124463 1101 40-41 0.20566518657268773 1101 42-43 0.26628889079426443 1101 44-45 0.4439030710240175 1101 46-47 0.3393077177913 1101 48-49 0.2469145892995499 1101 50-51 0.26852308987088236 1101 52-53 0.3163188507048318 1101 54-55 0.24732820256512866 1101 56-57 0.30694130377221995 1101 58-59 0.24183901507336003 1101 60-61 0.18520254065670372 1101 62-63 0.1236589059364519 1101 64-65 0.1602499582173138 1101 66-67 0.06036983015302155 1101 68-69 0.167511140011932 1101 70-71 0.21122560754835007 1101 72-73 0.14556577438118978 1101 74-75 0.08467512614186035 1101 76-77 0.07305293357190479 1101 78-79 0.1734432319793342 1101 80-81 0.05188838925599626 1101 82-83 0.17167363164652727 1101 84-85 0.13818283515134056 1101 86-87 -0.05802321052251358 1101 88-89 0.007319952661706708 1101 90-91 0.11519609710826373 1101 92-93 0.2014662649307546 1101 94-95 0.24288652659965138 1101 96-97 0.16534311380556588 1101 98-99 0.05004455032698374 1101 100-101 0.030544238590788808 1102 1 -0.09155352333551292 1102 2 -0.0322838294379153 1102 3 -0.017774157778148947 1102 4 0.004974362984768277 1102 5 -0.014470110404872116 1102 6 -0.1565173163867044 1102 7 -0.02715021752631941 1102 8 -0.032586550848485274 1102 9 -0.03037855444342341 1102 10-11 0.009459788555723492 1102 12-13 -0.11608153049569836 1102 14-15 0.011369037934379378 1102 16-17 -0.01237504955931712 1102 18-19 0.005699972046798507 1102 20-21 -0.08009831198371842 1102 22-23 -0.08092235739001552 1102 24-25 -0.06836048523989291 1102 26-27 -0.04810979822980954 1102 28-29 -0.10992660363005058 1102 30-31 -0.1384778059496483 1102 32-33 -0.12342327738924297 1102 34-35 -0.033247639384079264 1102 36-37 -0.1351654952303747 1102 38-39 -0.2224119651427543 1102 40-41 -0.15506610397819998 1102 42-43 -0.003949653786413876 1102 44-45 0.13231343407057494 1102 46-47 -0.03385473994816124 1102 48-49 -0.00512815121840049 1102 50-51 0.1200381910079571 1102 52-53 -0.10872353424004899 1102 54-55 -0.012011332753033344 1102 56-57 0.10118026528446933 1102 58-59 0.04481527436366406 1102 60-61 -0.1428351673702224 1102 62-63 -0.394711840954038 1102 64-65 -0.3335671061621923 1102 66-67 -0.16251974630596067 1102 68-69 -0.21754092229549116 1102 70-71 -0.12675026401310419 1102 72-73 -0.04453588495618277 1102 74-75 -0.03299243698602794 1102 76-77 -0.2473910307474121 1102 78-79 -0.12198177518736131 1102 80-81 -0.04878746208229856 1102 82-83 -0.06478470521865631 1102 84-85 -0.08576181225983959 1102 86-87 0.0016553715426255167 1102 88-89 -0.15349023989352872 1102 90-91 -0.21714392481235834 1102 92-93 -0.19939485408866275 1102 94-95 -0.27573938854164837 1102 96-97 -0.2591896455650833 1102 98-99 -0.28850640681645245 1102 100-101 -0.06303546353500167 1103 1 -0.1449455928850938 1103 2 -0.09338329024773984 1103 3 -0.0989267075770286 1103 4 0.024777306339792915 1103 5 0.010482800942881454 1103 6 -0.0967558820465868 1103 7 -0.08455750351062363 1103 8 -0.06010914007309509 1103 9 -0.05956916023676939 1103 10-11 -0.015348215194940451 1103 12-13 -0.08689300924930166 1103 14-15 -0.07923655327535783 1103 16-17 -0.12175002784528743 1103 18-19 -0.17282410301760365 1103 20-21 -0.20153378407485434 1103 22-23 -0.17177569384637792 1103 24-25 -0.0704666356767163 1103 26-27 -0.2364765986485864 1103 28-29 -0.23844612427522094 1103 30-31 -0.20554248211401926 1103 32-33 -0.1044229477781542 1103 34-35 -0.09283100288017465 1103 36-37 -0.2565705203024251 1103 38-39 -0.16911982751060606 1103 40-41 -0.20308035823658344 1103 42-43 0.05525646987978661 1103 44-45 0.20561741192940275 1103 46-47 0.05766558836418767 1103 48-49 0.0028816888538969465 1103 50-51 0.14980924837481524 1103 52-53 0.13378135086414744 1103 54-55 0.09119578694758701 1103 56-57 -0.06462087222121227 1103 58-59 0.04568351975249385 1103 60-61 0.04615983969765125 1103 62-63 -0.06822927642055987 1103 64-65 -0.01795539080619335 1103 66-67 0.01741051359661583 1103 68-69 -0.04080867325505011 1103 70-71 0.09622184765443365 1103 72-73 0.13062120460694615 1103 74-75 0.06392724693601082 1103 76-77 -0.04703138954721808 1103 78-79 0.27259112577808864 1103 80-81 0.27056039308944335 1103 82-83 0.31191276347176355 1103 84-85 0.16597553128701747 1103 86-87 0.17847435036982162 1103 88-89 0.08908779385819088 1103 90-91 0.2838433292568965 1103 92-93 0.22020574924239256 1103 94-95 0.19685936813348093 1103 96-97 0.17384996878615055 1103 98-99 0.12567273207362462 1103 100-101 0.08478797255891735 1104 1 0.0811691509565371 1104 2 0.011791888326186495 1104 3 0.09332702444954322 1104 4 0.0094575264400234 1104 5 -0.030412758266592732 1104 6 0.06987708542796867 1104 7 -0.01058888953932069 1104 8 -0.0016211719563443694 1104 9 0.08500625537600115 1104 10-11 0.055514509556417124 1104 12-13 0.029542627201266214 1104 14-15 0.10047726428357606 1104 16-17 0.1708382004546536 1104 18-19 0.14726568625435732 1104 20-21 0.026497998714141602 1104 22-23 0.04677397876279343 1104 24-25 0.0054903101082786065 1104 26-27 -0.007906741495013137 1104 28-29 0.08809237101134926 1104 30-31 0.11093473786795016 1104 32-33 0.10905904985908421 1104 34-35 0.06837313601645434 1104 36-37 0.11352584984950198 1104 38-39 0.111814918136794 1104 40-41 0.17990078674751686 1104 42-43 0.03483301091840474 1104 44-45 0.24541941645907883 1104 46-47 0.18899277064121378 1104 48-49 0.047765356699393635 1104 50-51 0.18681676329465802 1104 52-53 0.23901216489674226 1104 54-55 0.08655618709052249 1104 56-57 0.20705939175154953 1104 58-59 0.19880350858725393 1104 60-61 0.3077682576286733 1104 62-63 -0.03218640087486335 1104 64-65 0.09455961487180531 1104 66-67 0.22599754462149235 1104 68-69 0.24997799941389331 1104 70-71 0.20653662990043387 1104 72-73 0.23430797570296136 1104 74-75 0.11305160839990691 1104 76-77 0.12983858180211527 1104 78-79 0.11804440534531224 1104 80-81 0.31743771785682284 1104 82-83 0.36901652137676777 1104 84-85 0.3443034695704448 1104 86-87 0.34127633963948867 1104 88-89 0.18285213985860693 1104 90-91 0.22131598840291744 1104 92-93 0.33871515767532756 1104 94-95 0.4093861049453338 1104 96-97 0.31117075902584546 1104 98-99 0.2185633629352175 1104 100-101 0.46834995173154326 1105 1 -0.11866959918720354 1105 2 0.010718631584360594 1105 3 -0.10048946607060572 1105 4 -0.062426283063871324 1105 5 -0.1116910488613101 1105 6 -0.08453250140303226 1105 7 -0.06658161717950861 1105 8 0.04223905029142827 1105 9 -0.09573688780272249 1105 10-11 -0.1575225979469721 1105 12-13 -0.05005826415936809 1105 14-15 -0.05443976739491774 1105 16-17 0.0033925079427135074 1105 18-19 -0.09843235749131196 1105 20-21 -0.2046620768319869 1105 22-23 -0.07832467896935924 1105 24-25 -0.06304283888972861 1105 26-27 -0.015382079239849134 1105 28-29 -0.09247087970212675 1105 30-31 -0.011066763994570294 1105 32-33 -0.06581254263787883 1105 34-35 -0.07339062031245192 1105 36-37 -0.08004737204633017 1105 38-39 -0.13705302249956475 1105 40-41 0.10092668777790692 1105 42-43 0.03652696200159511 1105 44-45 0.03568810523901789 1105 46-47 -0.05544537248524506 1105 48-49 -0.14350214905890368 1105 50-51 -0.06661142955703525 1105 52-53 -0.08000817471235422 1105 54-55 -0.16335952915154195 1105 56-57 -0.059463491615410646 1105 58-59 0.04685905833513715 1105 60-61 0.16694164086148078 1105 62-63 0.10162397417261815 1105 64-65 -0.25196140423425817 1105 66-67 0.06605310656548369 1105 68-69 -0.04560187284068462 1105 70-71 0.001835120720251382 1105 72-73 0.009599854494638294 1105 74-75 0.022762578268562095 1105 76-77 0.06848588628992758 1105 78-79 0.07344239891274285 1105 80-81 0.09650407160642516 1105 82-83 0.2803286648586507 1105 84-85 0.19157062861106766 1105 86-87 0.20667256477251428 1105 88-89 0.3048353475721193 1105 90-91 0.16387954750347689 1105 92-93 0.2736284956780288 1105 94-95 0.14999271279195625 1105 96-97 0.16737243198221563 1105 98-99 0.43263430764507405 1105 100-101 0.3237948131664332 1106 1 -0.12293579065245908 1106 2 -0.009904244170925836 1106 3 -0.1347763492528813 1106 4 -0.1134789643327565 1106 5 -0.039173661524955605 1106 6 -0.0236296187242786 1106 7 -0.16816575300529024 1106 8 -0.01728897400363394 1106 9 -0.11980379364360516 1106 10-11 -0.10396704501393828 1106 12-13 -0.15334376264879523 1106 14-15 -0.10122625719854028 1106 16-17 -0.1475842512466201 1106 18-19 -0.09918174382062261 1106 20-21 -0.03487788435968042 1106 22-23 -0.08221224348346112 1106 24-25 -0.10869541473313404 1106 26-27 -0.008566794118998189 1106 28-29 -0.2115456137781635 1106 30-31 -0.11713935022316946 1106 32-33 -0.2264369132320283 1106 34-35 0.04262657820214599 1106 36-37 -0.11766715584541743 1106 38-39 -0.07654450191798645 1106 40-41 0.047496032566428426 1106 42-43 -0.024656202965680052 1106 44-45 0.09388552624858448 1106 46-47 0.018896308343052226 1106 48-49 0.001968292469292976 1106 50-51 0.11394740415394011 1106 52-53 -0.10165383495907321 1106 54-55 -0.09990755281719998 1106 56-57 -0.2221708021642499 1106 58-59 -0.09337452990755679 1106 60-61 -0.17862970229059272 1106 62-63 -0.11859922887371255 1106 64-65 -0.13210876387675796 1106 66-67 -0.32864827970088584 1106 68-69 -0.22772438668760486 1106 70-71 -0.08586668252748808 1106 72-73 -0.10188070473244437 1106 74-75 0.0779978580394598 1106 76-77 -0.024763539377243404 1106 78-79 -0.02728318800820162 1106 80-81 -0.006117600410188828 1106 82-83 -0.1544570224525259 1106 84-85 -0.024152322672925663 1106 86-87 0.05260581628308714 1106 88-89 0.045547677632541195 1106 90-91 0.20481354643348837 1106 92-93 0.18588932145849313 1106 94-95 0.030902243569901344 1106 96-97 -0.20498701382593865 1106 98-99 -0.07119523351806833 1106 100-101 -0.04825386350778871 1107 1 -0.09406071729565113 1107 2 -0.044397226369675025 1107 3 -0.07772920527791882 1107 4 -0.10009134696186095 1107 5 -0.130609865554689 1107 6 -0.024840006048222563 1107 7 0.0696111689430623 1107 8 0.01021677227961959 1107 9 -0.07934751430088482 1107 10-11 -0.0784006693292767 1107 12-13 -0.2449908675001211 1107 14-15 -0.05098601365394728 1107 16-17 0.023217905443523534 1107 18-19 -0.06914518767820965 1107 20-21 -0.06279931921883275 1107 22-23 -0.07480473419159495 1107 24-25 -0.04236404980746045 1107 26-27 0.06276353158522596 1107 28-29 0.044972444222629804 1107 30-31 -0.05945950378342246 1107 32-33 -0.16540404938226771 1107 34-35 -0.18074184413744376 1107 36-37 -0.29198279796201376 1107 38-39 0.06301529610626488 1107 40-41 0.09386670451478807 1107 42-43 0.11378490374402617 1107 44-45 -0.056433270210725084 1107 46-47 -0.14780550846054297 1107 48-49 0.15501351694112486 1107 50-51 0.00241754793328397 1107 52-53 -0.08765796739256615 1107 54-55 -0.1394101936622718 1107 56-57 -0.050427233111044245 1107 58-59 0.007854807924992713 1107 60-61 -0.19745183648464604 1107 62-63 -0.1749280874417849 1107 64-65 -0.21630098316470736 1107 66-67 -0.21103439325722206 1107 68-69 -0.08840147002094056 1107 70-71 -0.044630987104945063 1107 72-73 0.046818128894308586 1107 74-75 0.09971911439705394 1107 76-77 0.08859259569709366 1107 78-79 0.07396601729304919 1107 80-81 0.11409433963284599 1107 82-83 0.11707320055216641 1107 84-85 0.2884057514663567 1107 86-87 0.3075146093190817 1107 88-89 0.10016793389231893 1107 90-91 0.17819572317448618 1107 92-93 0.1352242197370508 1107 94-95 0.12033794632811734 1107 96-97 0.147952454581727 1107 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scores pass #Quality Count 3 3.0 4 9.0 5 32.0 6 65.0 7 195.0 8 436.0 9 802.0 10 1383.0 11 2339.0 12 3311.0 13 4991.0 14 6373.0 15 8835.0 16 11204.0 17 15157.0 18 13209.0 19 9037.0 20 7379.0 21 7347.0 22 7710.0 23 8720.0 24 10318.0 25 12461.0 26 15574.0 27 19773.0 28 25094.0 29 31808.0 30 40214.0 31 50068.0 32 63530.0 33 81361.0 34 110669.0 35 153140.0 36 228247.0 37 345285.0 38 349426.0 39 78828.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98338935783905 21.319427882737894 15.263384808567949 21.43379795085511 2 15.841423385179256 21.193732094598513 40.07892874955055 22.885915770671676 3 19.176190992732256 26.364869811168624 30.343711044428424 24.1152281516707 4 13.085741889950725 17.544290011499832 38.74445958038081 30.62550851816863 5 12.998173828980155 38.49352779242078 35.1447163693702 13.36358200922886 6 29.771168998818702 38.887526074694975 17.36503645093028 13.976268475556042 7 27.428810355996063 33.06836745878067 22.272849319723747 17.22997286549952 8 25.36116025941023 35.73223426718001 19.8672792951409 19.039326178268855 9 25.133541292980116 18.018955295634992 22.039953072798056 34.807550338586836 10-11 24.5525185096264 27.311405362992808 27.924816730486967 20.211259396893826 12-13 25.647735625568142 24.700521494050733 28.45577159227487 21.195971288106254 14-15 22.30661163135621 26.828380010409735 26.968954923529044 23.89605343470501 16-17 20.722604680077918 30.00829866855953 27.93337056425727 21.335726087105282 18-19 20.599342485645508 29.118149031561035 30.454083890202412 19.828424592591045 20-21 21.759190519717336 27.823593756524883 29.89900056143021 20.518215162327568 22-23 21.2815904679556 27.781518267799367 29.91136488451164 21.025526379733385 24-25 21.7031929866682 27.91864057254434 30.067189639364567 20.310976801422896 26-27 21.435568085006434 27.719418915462747 30.562004253337456 20.283008746193367 28-29 21.002844531519376 28.185308367342145 30.53235861222589 20.27948848891259 30-31 21.474600069480008 28.183625568874817 29.91592803077535 20.425846330869827 32-33 21.358969927611458 28.201693898878737 29.98420813939057 20.455128034119234 34-35 21.78598261491874 27.698023742463945 30.167149521151988 20.34884412146533 36-37 21.836703324748846 27.716874318461286 30.200430384445838 20.24599197234403 38-39 21.648017994158398 28.06954693486951 30.313276232319343 19.96915883865275 40-41 21.258564846361953 28.007446305698686 30.175805885713125 20.558182962226237 42-43 21.02069655082618 28.316382234448007 29.654955024708208 21.007966190017598 44-45 21.40081792771347 28.40534254166557 29.31307817290619 20.880761357714768 46-47 21.73465351817313 28.428078807449264 29.066394324898624 20.770873349478975 48-49 21.78651157922024 28.347929565772358 29.083651591416064 20.781907263591343 50-51 21.72507471662486 28.498183516847085 28.955051719533493 20.821690046994558 52-53 21.499723956194863 29.037749570276343 28.522023192319025 20.940503281209768 54-55 21.23267612102851 29.05099532926534 28.578151586043237 21.138176963662914 56-57 22.123125946488013 28.52212440194718 28.36323331614599 20.991516335418822 58-59 22.09148529999846 28.173173595530223 28.678393261108553 21.056947843362764 60-61 21.653981110995446 28.61892095887317 29.11128425339254 20.61581367673885 62-63 21.685512699469474 28.421717728222774 28.77608848453075 21.116681087777007 64-65 21.308888246554318 28.89930941294877 28.73893859242827 21.052863748068642 66-67 21.535863710849693 28.108505917309955 28.597155015042386 21.758475356797966 68-69 21.515740232076112 28.849492786705888 28.582007168916213 21.052759812301787 70-71 21.78727074513794 28.500063658745045 28.498671576848867 21.213994019268153 72-73 21.570909044515364 28.317282069809803 28.67961708936186 21.432191796312974 74-75 21.598467846227898 28.87500942373157 28.54077141216792 20.985751317872612 76-77 21.70163460124898 28.4133207257974 28.348514279600234 21.53653039335338 78-79 21.49490435461876 28.701994270932857 28.446102897618225 21.35699847683016 80-81 21.595258530701244 28.725846111020903 28.205044190308286 21.473851167969567 82-83 21.41323450652951 28.43579674471189 28.877756923061753 21.273211825696844 84-85 21.347100349520375 28.22648131512912 28.423394610067604 22.0030237252829 86-87 21.548929141744637 28.218352491410254 28.197735363651628 22.03498300319348 88-89 21.442752587103524 28.167264517689212 28.45510869038875 21.93487420481851 90-91 21.307288054206904 27.539034862870544 28.993635884933273 22.160041197989276 92-93 21.01528762558583 28.390973275143917 28.83058348607041 21.763155613199846 94-95 20.597761663175426 28.57057523099014 28.92326580510765 21.908397300726783 96-97 20.416452564413365 28.837514658513193 28.727090092294986 22.018942684778462 98-99 20.748500337253752 29.32237224810509 27.863394541650983 22.06573287299018 100-101 20.119523067696875 28.925475557741446 27.85607237270225 23.098929001859428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 145.0 1 111.5 2 104.0 3 126.0 4 113.5 5 119.5 6 198.5 7 417.0 8 677.5 9 892.5 10 984.0 11 992.5 12 1094.5 13 1289.0 14 1501.0 15 1684.0 16 1837.5 17 2074.0 18 2340.5 19 2515.5 20 2779.0 21 3137.5 22 3696.0 23 4586.5 24 5649.5 25 7017.0 26 9018.5 27 11469.0 28 15170.0 29 19859.0 30 24018.5 31 28301.0 32 33107.0 33 37763.5 34 42198.5 35 47543.0 36 51868.0 37 56040.5 38 60649.5 39 62601.0 40 63786.0 41 64762.0 42 66218.5 43 69633.0 44 71134.5 45 72099.0 46 75147.0 47 79689.5 48 94109.5 49 90592.0 50 72667.0 51 64100.5 52 53552.5 53 45062.0 54 39221.5 55 34124.0 56 27942.0 57 21767.5 58 17430.5 59 14584.5 60 11950.0 61 9105.5 62 6715.5 63 4865.5 64 3257.5 65 2110.5 66 1304.5 67 766.0 68 452.5 69 264.5 70 143.5 71 72.0 72 44.0 73 26.0 74 13.5 75 9.5 76 9.5 77 6.5 78 3.5 79 4.0 80 3.0 81 1.5 82 1.5 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05915193522515141 2 0.0019137390808137218 3 0.026328410384528173 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.8996046678995784E-5 14-15 0.001478798380628785 16-17 0.0 18-19 0.0 20-21 0.011888379138388272 22-23 0.0 24-25 0.0 26-27 0.004639367468639325 28-29 0.01087351750462342 30-31 0.007886924696686854 32-33 0.003885470254985436 34-35 0.0045523793286023385 36-37 0.01931136708821119 38-39 0.04073944558398908 40-41 0.001623778614023764 42-43 0.008611825863661748 44-45 0.007973912836723841 46-47 0.004001454441701418 48-49 7.249011669748946E-4 50-51 0.0014208062872707935 52-53 5.219288402219241E-4 54-55 0.00110184977380184 56-57 0.013686134032486011 58-59 0.010003636104253547 60-61 0.0021167114075666925 62-63 0.002841612574541587 64-65 0.08646621119676542 66-67 0.04311712141166673 68-69 0.031431714600031434 70-71 0.019514339414964162 72-73 1.1598418671598314E-4 74-75 1.739762800739747E-4 76-77 4.349407001849368E-4 78-79 4.929327935429284E-4 80-81 7.538972136538904E-4 82-83 5.5092488690092E-4 84-85 5.799209335799157E-4 86-87 0.0045523793286023385 88-89 1.4498023339497892E-4 90-91 9.858655870858568E-4 92-93 2.6096442011096205E-4 94-95 0.00330554932140552 96-97 0.006901059109600997 98-99 0.00881479819041472 100-101 0.010844521457944425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1724373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.02338753545143 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84101197770943 44.481463308075774 2 11.508761296599157 12.6650206575156 3 3.064838374340296 5.059133688145472 4 1.2838501419257107 2.825671355864908 5 0.7146252442759025 1.9660550879204812 6 0.4506828383220375 1.4878857881142404 7 0.3148870690071297 1.2128307259517215 8 0.23534728759195953 1.0359684008471783 9 0.18093529793413485 0.8960105715365041 >10 1.2135401428590658 12.897915390712408 >50 0.11940859961308382 4.583810172842682 >100 0.06637707485578402 6.463134952752049 >500 0.0030797221115419354 1.1859050359153969 >1k 0.002442538226395328 2.4252181626327975 >5k 2.1239462838220242E-4 0.8139767011727755 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8631 0.5005297577728253 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5299 0.3073001027039973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4392 0.254701274028299 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3792 0.219906018013504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2771 0.16069609069499466 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2591 0.15025751389055617 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2236 0.12967032074846915 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2178 0.12630677933370565 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1889 0.10954706435324607 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1817 0.10537163363147069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7992093357991574E-5 2 0.0 0.0 0.0 0.0 5.7992093357991574E-5 3 0.0 0.0 0.0 0.0 5.7992093357991574E-5 4 0.0 0.0 0.0 0.0 5.7992093357991574E-5 5 0.0 0.0 0.0 0.0 5.7992093357991574E-5 6 0.0 0.0 0.0 0.0 5.7992093357991574E-5 7 0.0 0.0 0.0 0.0 5.7992093357991574E-5 8 0.0 0.0 0.0 0.0 5.7992093357991574E-5 9 0.0 0.0 0.0 0.0 5.7992093357991574E-5 10-11 0.0 0.0 0.0 1.1598418671598314E-4 5.7992093357991574E-5 12-13 0.0 0.0 0.0 1.739762800739747E-4 5.7992093357991574E-5 14-15 0.0 0.0 0.0 2.8996046678995784E-4 5.7992093357991574E-5 16-17 0.0 0.0 0.0 2.8996046678995784E-4 5.7992093357991574E-5 18-19 0.0 0.0 0.0 2.8996046678995784E-4 5.7992093357991574E-5 20-21 0.0 0.0 0.0 5.219288402219241E-4 5.7992093357991574E-5 22-23 0.0 0.0 0.0 7.538972136538905E-4 5.7992093357991574E-5 24-25 0.0 0.0 0.0 0.0011598418671598314 5.7992093357991574E-5 26-27 0.0 0.0 0.0 0.001623778614023764 5.7992093357991574E-5 28-29 0.0 0.0 0.0 0.004175430721775394 5.7992093357991574E-5 30-31 0.0 0.0 0.0 0.008698814003698735 5.7992093357991574E-5 32-33 0.0 0.0 0.0 0.015019952179719818 5.7992093357991574E-5 34-35 0.0 0.0 0.0 0.021051129888950942 5.7992093357991574E-5 36-37 0.0 2.8996046678995787E-5 0.0 0.02792319295187294 5.7992093357991574E-5 38-39 0.0 5.7992093357991574E-5 0.0 0.038129801382879455 5.7992093357991574E-5 40-41 0.0 5.7992093357991574E-5 0.0 0.052975777282525296 5.7992093357991574E-5 42-43 0.0 5.7992093357991574E-5 0.0 0.07466482019841415 5.7992093357991574E-5 44-45 0.0 5.7992093357991574E-5 0.0 0.09078662215193581 5.7992093357991574E-5 46-47 0.0 5.7992093357991574E-5 0.0 0.12363914303923804 5.7992093357991574E-5 48-49 0.0 5.7992093357991574E-5 0.0 0.16379866768964718 5.7992093357991574E-5 50-51 0.0 5.7992093357991574E-5 0.0 0.1802974182499958 5.7992093357991574E-5 52-53 0.0 5.7992093357991574E-5 0.0 0.20877153608876964 5.7992093357991574E-5 54-55 0.0 5.7992093357991574E-5 0.0 0.2594276296369753 5.7992093357991574E-5 56-57 0.0 5.7992093357991574E-5 0.0 0.33171477400771177 5.7992093357991574E-5 58-59 0.0 5.7992093357991574E-5 0.0 0.44535028094269624 5.7992093357991574E-5 60-61 0.0 5.7992093357991574E-5 0.0 0.585314198262209 5.7992093357991574E-5 62-63 0.0 5.7992093357991574E-5 0.0 0.7198558548527494 5.7992093357991574E-5 64-65 0.0 5.7992093357991574E-5 0.0 0.8783192499534613 5.7992093357991574E-5 66-67 0.0 5.7992093357991574E-5 0.0 1.1599578513465474 5.7992093357991574E-5 68-69 0.0 5.7992093357991574E-5 0.0 1.4665910449769277 5.7992093357991574E-5 70-71 0.0 5.7992093357991574E-5 0.0 1.5752102358364461 5.7992093357991574E-5 72-73 0.0 5.7992093357991574E-5 0.0 1.72033544946482 5.7992093357991574E-5 74-75 0.0 5.7992093357991574E-5 0.0 1.871810797315894 5.7992093357991574E-5 76-77 0.0 5.7992093357991574E-5 0.0 2.0157761690771077 5.7992093357991574E-5 78-79 0.0 5.7992093357991574E-5 0.0 2.1777480858259786 5.7992093357991574E-5 80-81 0.0 5.7992093357991574E-5 0.0 2.4795679357076454 5.7992093357991574E-5 82-83 0.0 5.7992093357991574E-5 0.0 2.898908762779283 5.7992093357991574E-5 84-85 0.0 5.7992093357991574E-5 0.0 3.724687176150404 5.7992093357991574E-5 86-87 0.0 5.7992093357991574E-5 0.0 4.677468273975526 5.7992093357991574E-5 88-89 0.0 5.7992093357991574E-5 0.0 5.657853608239053 5.7992093357991574E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCG 195 5.3842086E-10 26.784878 9 TCACGCA 150 1.0643507E-6 25.32315 4 CGTGACG 135 1.8189894E-12 24.664785 64-65 ACGGGCG 330 0.0 23.021046 5 GGCGTCG 340 0.0 22.343958 8 TACGTGC 255 1.1983502E-8 20.481958 8 GAGTACT 6120 0.0 20.133419 12-13 GTACTTT 6175 0.0 19.881521 14-15 AGTACTT 6100 0.0 19.771313 12-13 GAGTACG 245 1.7438288E-7 19.401901 1 GTACATG 7680 0.0 18.568228 1 GCGTCGG 435 0.0 18.556293 9 GCATCGT 540 0.0 18.464796 8 CGGGCGT 415 1.8189894E-12 18.305891 6 TACATGG 7610 0.0 18.101778 2 TATACGG 555 0.0 17.973568 2 CAATGCG 240 0.0 17.81309 18-19 GTATACG 620 0.0 17.633825 1 CTAACGC 165 0.0014638905 17.273298 3 GAGCGTA 165 0.0014681624 17.265785 7 >>END_MODULE