##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1175921 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88238070414594 31.0 31.0 34.0 30.0 34.0 2 32.07487152623348 33.0 31.0 34.0 30.0 34.0 3 32.12198523540272 33.0 31.0 34.0 30.0 34.0 4 35.85330732251571 37.0 35.0 37.0 35.0 37.0 5 35.73647804571906 37.0 35.0 37.0 35.0 37.0 6 35.81031208729158 37.0 35.0 37.0 35.0 37.0 7 35.7648277392784 37.0 35.0 37.0 33.0 37.0 8 35.76256058017503 37.0 35.0 37.0 33.0 37.0 9 37.39525104152405 39.0 37.0 39.0 34.0 39.0 10-11 37.30514890030878 39.0 37.0 39.0 34.0 39.0 12-13 37.27437982653596 39.0 37.0 39.0 34.0 39.0 14-15 38.45857162173309 40.0 38.0 41.0 34.0 41.0 16-17 38.46660574987605 40.0 38.0 41.0 34.0 41.0 18-19 38.49315855401851 40.0 38.0 41.0 34.0 41.0 20-21 38.43103873474494 40.0 38.0 41.0 34.0 41.0 22-23 38.33169532647176 40.0 38.0 41.0 34.0 41.0 24-25 38.21971671566372 40.0 37.5 41.0 33.5 41.0 26-27 38.037566299096625 40.0 37.0 41.0 33.0 41.0 28-29 37.88494635268866 40.0 37.0 41.0 33.0 41.0 30-31 37.66598181340413 40.0 37.0 41.0 33.0 41.0 32-33 37.366880938430384 39.0 36.0 41.0 32.0 41.0 34-35 37.27555465035492 39.0 36.0 41.0 32.0 41.0 36-37 37.100227821426785 39.0 36.0 41.0 31.5 41.0 38-39 36.86655098429232 39.0 36.0 41.0 31.0 41.0 40-41 36.85333155883771 39.0 36.0 41.0 31.0 41.0 42-43 36.6993063309525 39.0 36.0 41.0 30.0 41.0 44-45 36.43238448841376 39.0 35.0 41.0 30.0 41.0 46-47 36.2146543007566 39.0 35.0 41.0 30.0 41.0 48-49 35.9461396641441 39.0 35.0 41.0 28.5 41.0 50-51 35.69316518711716 39.0 35.0 41.0 28.0 41.0 52-53 35.510314043205284 39.0 35.0 41.0 27.0 41.0 54-55 35.33119359208654 39.0 35.0 40.0 27.0 41.0 56-57 35.049795862137 38.5 34.5 40.0 26.0 41.0 58-59 34.789828568415736 38.0 34.0 40.0 26.0 41.0 60-61 34.43609732286437 38.0 34.0 40.0 25.0 41.0 62-63 34.188286458018865 37.5 34.0 40.0 25.0 41.0 64-65 33.74774793544805 37.0 33.0 39.5 23.0 41.0 66-67 33.37388013310418 36.5 33.0 39.0 22.0 41.0 68-69 33.04717536297081 36.0 32.5 39.0 22.0 40.5 70-71 32.62323361858492 35.5 32.0 39.0 21.5 40.0 72-73 32.22156080212872 35.0 32.0 38.0 20.5 40.0 74-75 31.847358368461826 35.0 31.0 37.0 19.5 39.5 76-77 30.866215502572025 34.0 30.5 36.0 18.5 39.0 78-79 31.277301366333283 35.0 31.0 36.0 18.5 39.0 80-81 31.05383439873937 35.0 31.0 36.0 18.0 38.5 82-83 30.749249311816016 35.0 31.0 35.5 15.5 37.0 84-85 30.420742549882178 34.0 31.0 35.0 11.5 37.0 86-87 30.145769996453843 34.0 31.0 35.0 7.5 36.5 88-89 29.82635143007056 34.0 30.5 35.0 4.5 36.0 90-91 29.541736222076143 34.0 30.0 35.0 2.0 36.0 92-93 29.305081718924995 34.0 30.0 35.0 2.0 35.5 94-95 28.97730672383604 34.0 29.5 35.0 2.0 35.0 96-97 28.651670903062367 34.0 29.0 35.0 2.0 35.0 98-99 28.339797911594403 34.0 29.0 35.0 2.0 35.0 100-101 27.457154859892796 33.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.07822259070247028 1101 2 0.10628247727341744 1101 3 0.0723946629032497 1101 4 0.054106546471160755 1101 5 0.08549278380021264 1101 6 0.12475721153765562 1101 7 0.17172436868833074 1101 8 0.04631407313588909 1101 9 0.111912117718731 1101 10-11 0.13102828154652713 1101 12-13 0.12285542813426531 1101 14-15 0.06808015170171444 1101 16-17 0.07551946519343744 1101 18-19 0.1256770779070635 1101 20-21 0.11355046654281153 1101 22-23 0.08729407371846065 1101 24-25 0.26221214818052374 1101 26-27 0.16326752185713644 1101 28-29 0.1593218793675817 1101 30-31 0.12489210264112671 1101 32-33 0.25542912459113865 1101 34-35 0.24290847634261326 1101 36-37 0.24499242057081716 1101 38-39 0.1456100932946569 1101 40-41 0.3459822411184632 1101 42-43 0.2907229891889429 1101 44-45 0.3577713362504724 1101 46-47 0.14886351539821163 1101 48-49 0.20271911236440587 1101 50-51 0.25881582853929785 1101 52-53 0.15964764507749152 1101 54-55 0.21418056193510182 1101 56-57 0.10822763953683534 1101 58-59 -0.035606644170762536 1101 60-61 0.009830902657860463 1101 62-63 -0.10315126480662684 1101 64-65 -0.05627526739680633 1101 66-67 -0.16453404315649323 1101 68-69 -0.05743414701684202 1101 70-71 -0.03626356351762894 1101 72-73 0.19622896547876678 1101 74-75 0.0766399650848335 1101 76-77 0.04755532096887549 1101 78-79 0.06960318290495948 1101 80-81 0.05201120701178752 1101 82-83 0.1545412633990857 1101 84-85 0.1803851345815346 1101 86-87 -0.10935543658645841 1101 88-89 0.12044966253976597 1101 90-91 0.15812536399437604 1101 92-93 0.16752458147298555 1101 94-95 0.12105888427602451 1101 96-97 0.04918406858971025 1101 98-99 -0.18195977386584872 1101 100-101 -0.09753284307112153 1102 1 0.0050742537507737495 1102 2 0.016381912328775172 1102 3 0.01201215579646231 1102 4 -0.0032983065352780727 1102 5 0.12773648964516582 1102 6 0.007585173298934933 1102 7 -0.04025401708130261 1102 8 -0.035090731892239546 1102 9 0.011317186627586295 1102 10-11 0.06856801358764386 1102 12-13 -0.08539234387278327 1102 14-15 -0.08205808581492846 1102 16-17 0.056521852114556737 1102 18-19 -0.001165289342942799 1102 20-21 -0.010209593909863202 1102 22-23 -0.1289660766935512 1102 24-25 -0.027769979897591668 1102 26-27 -0.23887950768551036 1102 28-29 -0.1947787671401997 1102 30-31 -0.14667504900632622 1102 32-33 -0.08012190634288174 1102 34-35 -0.06995750823236335 1102 36-37 -0.25328036013230104 1102 38-39 -0.15406065552687664 1102 40-41 -0.23845419361304465 1102 42-43 -0.17870432966294203 1102 44-45 -0.11419643431722903 1102 46-47 -0.38156013311266435 1102 48-49 -0.5210133605578307 1102 50-51 -0.30806654062983796 1102 52-53 -0.13946985381300436 1102 54-55 -0.22210873530254105 1102 56-57 -0.3170449372584514 1102 58-59 -0.22381897219849378 1102 60-61 -0.30792437195800915 1102 62-63 -0.39679233904107036 1102 64-65 -0.5631853440421608 1102 66-67 -0.5105743329455166 1102 68-69 -0.6064155074419304 1102 70-71 -0.5656106986125664 1102 72-73 -0.4359989674955287 1102 74-75 -0.5961890162156216 1102 76-77 -0.4402383962709351 1102 78-79 -0.23884579815559803 1102 80-81 -0.3190857571604049 1102 82-83 -0.35684129168601686 1102 84-85 -0.37483220717998833 1102 86-87 -0.3270918197412733 1102 88-89 -0.295464144419487 1102 90-91 -0.15393428504425444 1102 92-93 -0.21622276472653468 1102 94-95 -0.19493168798531713 1102 96-97 -0.04195613603779691 1102 98-99 -0.3797935529976826 1102 100-101 -0.37508498950093383 1103 1 -0.03744661989309961 1103 2 0.027427941003523415 1103 3 -0.018767528444968207 1103 4 -0.008670117140368916 1103 5 0.07562076671565165 1103 6 0.01862304575576701 1103 7 -0.03645008252177462 1103 8 -0.08525272692823904 1103 9 -0.054596347424002545 1103 10-11 -0.022259315514496336 1103 12-13 -0.017539198458713656 1103 14-15 -0.07429305265048924 1103 16-17 -0.01317804925794519 1103 18-19 -0.24294789978440434 1103 20-21 -0.18452177377831447 1103 22-23 -0.2753328580491896 1103 24-25 -0.2775472524081266 1103 26-27 -0.49467355941852276 1103 28-29 -0.2864875951795156 1103 30-31 -0.48112923490284487 1103 32-33 -0.30974234105674725 1103 34-35 -0.2989746965205313 1103 36-37 -0.5256955291918857 1103 38-39 -0.27610465393076566 1103 40-41 -0.17088996653389898 1103 42-43 -0.05196329083683082 1103 44-45 -0.010674265716907883 1103 46-47 -0.19270416188278006 1103 48-49 -0.12907697403843343 1103 50-51 0.03859718979837368 1103 52-53 0.10648497579732208 1103 54-55 0.09374927372701336 1103 56-57 -0.15740391123133435 1103 58-59 -0.24352125769623711 1103 60-61 -0.2525634153967786 1103 62-63 -0.1533474286465335 1103 64-65 -0.13658628614808066 1103 66-67 -0.1332993755460663 1103 68-69 -0.2364196608390543 1103 70-71 -0.281338898296859 1103 72-73 -0.3200724462354074 1103 74-75 -0.39748002003273086 1103 76-77 -0.3831781230752753 1103 78-79 -0.329770060770759 1103 80-81 -0.30180241767853744 1103 82-83 -0.08835585116241518 1103 84-85 -0.3015696070780969 1103 86-87 -0.2877386956588488 1103 88-89 -0.43286818337860566 1103 90-91 -0.32891935289144314 1103 92-93 -0.264707088475685 1103 94-95 -0.1847470814036214 1103 96-97 -0.14916585624187917 1103 98-99 -0.22384415668328472 1103 100-101 -0.18962639673713255 1104 1 0.04880355106238454 1104 2 0.03440677719638785 1104 3 0.034977022650011236 1104 4 -0.0037986082439402935 1104 5 -0.1555399391426775 1104 6 -0.11073872686477415 1104 7 -0.07212247210379985 1104 8 -0.2102727118648957 1104 9 -0.025864268855748662 1104 10-11 -0.14442843719819365 1104 12-13 0.059662371057086716 1104 14-15 0.01714645992893793 1104 16-17 0.017137414171060072 1104 18-19 -0.03757305942311007 1104 20-21 0.023343719411400343 1104 22-23 -0.10611100887911817 1104 24-25 0.07784078282700335 1104 26-27 6.860827596213426E-4 1104 28-29 -0.013148560119681463 1104 30-31 -0.07989527341646863 1104 32-33 -0.07523187087620187 1104 34-35 -0.08036645714048518 1104 36-37 0.025914726981298486 1104 38-39 0.20591660811557233 1104 40-41 -0.007665524619277164 1104 42-43 -0.1928219400284732 1104 44-45 -0.045395083375311174 1104 46-47 -0.05109812421671478 1104 48-49 -0.13644684079909553 1104 50-51 -0.23833046569406235 1104 52-53 -0.3762229975663658 1104 54-55 -0.29320918264610896 1104 56-57 -0.2813567487070898 1104 58-59 -0.3815126704338141 1104 60-61 -0.24225022495332382 1104 62-63 -0.4105646393850435 1104 64-65 -0.34480428472650004 1104 66-67 -0.5026277468363318 1104 68-69 -0.4949650271268311 1104 70-71 -0.2816162795863377 1104 72-73 -0.361204816824511 1104 74-75 -0.47915628910028474 1104 76-77 -0.5208372561431744 1104 78-79 -0.4894116610296493 1104 80-81 -0.436627060694601 1104 82-83 -0.49565560209713055 1104 84-85 -0.5178990821147487 1104 86-87 -0.6173890218707996 1104 88-89 -0.6034316719106236 1104 90-91 -0.723068533942449 1104 92-93 -0.45071532130645764 1104 94-95 -0.2119718623398228 1104 96-97 -0.16831287025156172 1104 98-99 -0.3439271399190531 1104 100-101 -0.10485077701147105 1105 1 -0.08957635867523805 1105 2 -0.048699986740153633 1105 3 -0.044825645029284544 1105 4 0.05819038723641512 1105 5 0.009503714206474001 1105 6 0.02780364732151952 1105 7 -0.05567049992240669 1105 8 0.07678826711063635 1105 9 0.049867000591866883 1105 10-11 -0.04922198664186794 1105 12-13 -0.1261777104230788 1105 14-15 -0.047621395338069306 1105 16-17 -0.0039614327228321145 1105 18-19 -0.12581017661217686 1105 20-21 -0.22696734454804357 1105 22-23 -0.05969837397326927 1105 24-25 -0.20624951086826115 1105 26-27 -0.009404003450931953 1105 28-29 -0.07484916489195825 1105 30-31 -0.048650228092803616 1105 32-33 0.12972453449674504 1105 34-35 0.05452894903042704 1105 36-37 0.12228354868341995 1105 38-39 0.08514060222248787 1105 40-41 0.10480848270011478 1105 42-43 0.33016743192572306 1105 44-45 0.4717481782974957 1105 46-47 0.34645858011690933 1105 48-49 0.49332375809444784 1105 50-51 0.46039422100727734 1105 52-53 0.3648383585354793 1105 54-55 0.472316122333595 1105 56-57 0.37059611963864114 1105 58-59 0.5050897542665211 1105 60-61 0.48760369406107884 1105 62-63 0.5486267704866634 1105 64-65 0.42662030664931905 1105 66-67 0.37828972709595377 1105 68-69 0.2924694895871127 1105 70-71 0.4711144607100053 1105 72-73 0.39760202860524174 1105 74-75 0.32209520912858736 1105 76-77 0.4364036016430184 1105 78-79 0.5013213407196169 1105 80-81 0.6366708305951541 1105 82-83 0.7549079668488012 1105 84-85 0.7963534868135689 1105 86-87 0.6242915260454467 1105 88-89 0.6854483869158621 1105 90-91 0.6552710402441058 1105 92-93 0.7470469755885141 1105 94-95 0.64532432470439 1105 96-97 0.686482081997589 1105 98-99 0.5549371096158993 1105 100-101 0.6595490212455246 1106 1 -0.16339234424489035 1106 2 -0.06831142873004836 1106 3 -0.1402362830112338 1106 4 -0.12885625842265824 1106 5 0.019212165088845268 1106 6 -0.09086382775258528 1106 7 -0.2060005996259946 1106 8 -0.02279433068191139 1106 9 -0.0918448983956992 1106 10-11 -0.20266232397325012 1106 12-13 -0.14152954638786497 1106 14-15 -0.12504049960062957 1106 16-17 -0.1998074146079034 1106 18-19 -0.17507594193205023 1106 20-21 -0.20348090874495028 1106 22-23 -0.13588011991335947 1106 24-25 -0.2765132469204872 1106 26-27 -0.3674391334285545 1106 28-29 -0.16637552887328155 1106 30-31 -0.26847621426738755 1106 32-33 -0.2918740362169103 1106 34-35 -0.3252500037794732 1106 36-37 -0.2257355093844282 1106 38-39 -0.18383463136326839 1106 40-41 -0.16595129655516416 1106 42-43 -0.18555801751030998 1106 44-45 -0.2007983463754286 1106 46-47 -0.5253789382372318 1106 48-49 -0.47439218842967534 1106 50-51 -0.5368791224448941 1106 52-53 -0.5313452159452439 1106 54-55 -0.39603548265763777 1106 56-57 -0.5643758885664241 1106 58-59 -0.41815656527989375 1106 60-61 -0.5620862101948845 1106 62-63 -0.6134788559191122 1106 64-65 -0.49769976495248613 1106 66-67 -0.5754255100093957 1106 68-69 -0.6439619140171757 1106 70-71 -0.458249220898459 1106 72-73 -0.33077272873503816 1106 74-75 -0.31468753549714634 1106 76-77 -0.5857873887385807 1106 78-79 -0.5558174606376269 1106 80-81 -0.3496041159234693 1106 82-83 -0.39184204277509593 1106 84-85 -0.5472286105362834 1106 86-87 -0.4195119897020021 1106 88-89 -0.5967514574519548 1106 90-91 -0.496503331590322 1106 92-93 -0.5677163590096761 1106 94-95 -0.5240683110888114 1106 96-97 -0.5592459383940742 1106 98-99 -0.5112784024579682 1106 100-101 -0.19139810780819744 1107 1 0.011669872186114816 1107 2 -0.01229096187203993 1107 3 0.018897646660718692 1107 4 0.023134520632709155 1107 5 -0.08879277113931039 1107 6 0.05067341699863448 1107 7 -0.0453376718146572 1107 8 -0.0370395455927266 1107 9 0.12269760540478103 1107 10-11 0.0404854312461822 1107 12-13 -0.0010513749639855519 1107 14-15 0.023864770013680925 1107 16-17 0.08920735005550995 1107 18-19 0.14924958206530192 1107 20-21 0.020417537605119662 1107 22-23 0.06429207579839868 1107 24-25 0.09959424957250462 1107 26-27 0.05525254893895948 1107 28-29 0.12365392121564156 1107 30-31 0.19922883582858475 1107 32-33 -0.20314995728421792 1107 34-35 -0.06429683044801493 1107 36-37 -0.046277423302719 1107 38-39 0.14595390613974502 1107 40-41 0.27595301046530807 1107 42-43 0.025913283693512312 1107 44-45 0.15326633816966506 1107 46-47 -0.011317511250567236 1107 48-49 0.08922117753793657 1107 50-51 -0.10640855199066124 1107 52-53 -0.042990987282252036 1107 54-55 0.01282368223351682 1107 56-57 0.11003921828736196 1107 58-59 0.03961586638475012 1107 60-61 -0.04624339766003516 1107 62-63 0.1595742393483306 1107 64-65 0.07761916762485299 1107 66-67 0.05069115753769182 1107 68-69 0.16272253715759888 1107 70-71 0.03945104557568868 1107 72-73 0.024699162350991344 1107 74-75 -0.017616916913219427 1107 76-77 -0.05942427662574801 1107 78-79 0.17147610993987072 1107 80-81 0.16154348375488325 1107 82-83 0.14100133616405586 1107 84-85 0.04829342053671226 1107 86-87 0.1805863973663726 1107 88-89 -0.13467816825707146 1107 90-91 -0.10896833484787649 1107 92-93 0.12113838910182295 1107 94-95 0.0766522402896328 1107 96-97 0.1951506649409609 1107 98-99 0.22535937171986475 1107 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72-73 -0.6538886103077957 2316 74-75 -0.26652031263315834 2316 76-77 -0.18699123505698623 2316 78-79 -0.13093886139919064 2316 80-81 -0.24883613577543784 2316 82-83 -0.5064347351979777 2316 84-85 -0.23688654918344199 2316 86-87 -0.2933487885724908 2316 88-89 -0.4308843244352296 2316 90-91 -0.37022514736065304 2316 92-93 -0.31753452526988823 2316 94-95 -0.14777675673122204 2316 96-97 -0.39515435836583634 2316 98-99 -0.4293061481474396 2316 100-101 -0.5339247411030215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 14.0 6 55.0 7 170.0 8 398.0 9 747.0 10 1383.0 11 2583.0 12 4185.0 13 5945.0 14 6025.0 15 7309.0 16 8955.0 17 11838.0 18 10249.0 19 6991.0 20 5931.0 21 5893.0 22 5841.0 23 6554.0 24 7601.0 25 8929.0 26 11065.0 27 14021.0 28 17355.0 29 22094.0 30 27675.0 31 34102.0 32 43781.0 33 55908.0 34 75020.0 35 103434.0 36 155024.0 37 231462.0 38 227036.0 39 50299.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79556621954488 20.04849846208824 13.590460267422221 20.565475050944656 2 15.845763041945643 23.170496284056437 38.512976250107364 22.470764423890554 3 21.168120422540603 26.970694901830004 30.06922390449364 21.79196077113575 4 12.604843352572153 17.658244048707353 39.611844673239105 30.125067925481392 5 11.695683638611778 38.78772468558687 34.42076466021102 15.095827015590332 6 27.090935530533088 39.21309339658021 18.426407896448826 15.269563176437872 7 26.557906526033637 32.888688951043484 23.223413817764968 17.329990705157915 8 24.566191096170577 32.725497716258154 20.88652213881715 21.821789048754127 9 23.941829425616177 19.62036565381518 23.764606635990003 32.67319828457864 10-11 24.328037342644617 26.14499613494444 28.688661908410513 20.83830461400043 12-13 27.061724384546242 24.1347420447462 28.21669142740031 20.586842143307248 14-15 22.237057169730132 26.6928706643921 28.977797469517018 22.09227469636075 16-17 20.106537769118844 29.287766780251395 29.14634571540095 21.459349735228813 18-19 19.57640011531387 29.871521981493654 30.645085851855697 19.906992051336783 20-21 21.63034719136242 27.40024281495247 29.71119478989703 21.258215203788083 22-23 20.44321004557279 27.78724080954418 30.140119957037932 21.6294291878451 24-25 22.030944255609008 27.61405740691764 30.2859630876564 20.069035249816952 26-27 21.49245226857167 26.20423523408598 32.421992601097074 19.88131989624527 28-29 20.157412943616944 29.282694357101484 30.810300732887043 19.74959196639453 30-31 21.377845741423656 27.653402982840504 30.02184910256236 20.946902173173488 32-33 21.266576774592437 27.939978968486322 30.606933085683647 20.186511171237598 34-35 22.154909472217863 26.526496262930486 31.00175234265804 20.316841922193603 36-37 22.22722642901646 26.82002504938642 31.443529369236035 19.509219152361084 38-39 21.148812625623464 28.333336878263548 31.177930682434518 19.33991981367847 40-41 20.679595054521705 28.59685065084168 30.128960711375996 20.59459358326062 42-43 20.264166749945247 28.790220897324954 29.033720383832755 21.91189196889704 44-45 21.59807549522318 27.485386334042932 29.06233548197244 21.854202688761447 46-47 22.413798235419932 28.933023942891182 28.761022247111796 19.892155574577085 48-49 22.082533062846515 28.379983238434754 29.309097805136386 20.228385893582345 50-51 20.319875363074356 28.768415220570194 30.509556333874905 20.40215308248054 52-53 21.257603679190005 29.77691338885341 28.225605509260483 20.739877422696104 54-55 20.576829859428017 29.048013878613162 28.558945837691663 21.81621042426716 56-57 22.085658165940426 27.91989124014555 28.699359543256236 21.295091050657785 58-59 23.493697196694068 27.516443759535854 28.295938344619014 20.693920699151064 60-61 21.794831998931855 28.727142997464433 29.99973211335772 19.478292890245992 62-63 20.946164299916486 28.687129743492925 29.508008511115985 20.8586974454746 64-65 20.67496041696541 30.495978295604104 28.443702733856906 20.385358553573578 66-67 21.671037711211625 28.098684943672648 27.83089289253696 22.399384452578776 68-69 21.156507545232696 29.203720857437165 27.990299776377693 21.64947182095245 70-71 22.909684418322097 28.644494426746515 27.821189802759854 20.62463135217153 72-73 22.14751003469624 27.44608476767127 30.05127899857133 20.35512619906116 74-75 20.686008718294982 30.343824994599967 28.543359315870664 20.426806971234388 76-77 21.560927157668083 28.40027331872629 27.89985513427186 22.138944389333766 78-79 21.79351169917252 29.052062795275003 28.373698302558047 20.78072720299443 80-81 22.637137888367782 28.230101665511537 28.374202216996974 20.758558229123704 82-83 21.69399076719373 27.873346341637646 29.682362380630238 20.750300510538384 84-85 20.859511596105833 29.016244412847218 29.15341454122417 20.970829449822777 86-87 21.241701945269835 29.47053180687112 27.8330319982175 21.454734249641543 88-89 20.74338421975314 29.171342085916596 27.50256288802082 22.582710806309443 90-91 21.546018363634666 27.276128890706865 28.04812609117527 23.129726654483203 92-93 22.082085725438105 28.778221592170194 28.156961758355393 20.982730924036304 94-95 21.477190681868628 28.418463799248407 28.71249365380249 21.39185186508048 96-97 19.642844234520123 28.919692466919273 30.12573206216485 21.31173123639575 98-99 20.783421092132496 29.899592254320368 27.76935601355779 21.54763063998935 100-101 19.673653607453076 29.44240121819658 27.729110297222448 23.1548348771279 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 296.0 1 228.5 2 484.0 3 686.0 4 447.5 5 347.0 6 453.0 7 694.5 8 950.0 9 1128.0 10 1182.5 11 1206.5 12 1310.0 13 1469.0 14 1643.5 15 1789.5 16 1978.5 17 2146.0 18 2263.0 19 2447.0 20 2738.0 21 3056.5 22 3515.0 23 4096.5 24 4767.0 25 5779.0 26 7111.0 27 8821.0 28 11188.0 29 13977.0 30 16578.0 31 19092.5 32 21765.0 33 24357.5 34 26947.5 35 29671.0 36 32154.5 37 34693.0 38 37259.5 39 38604.0 40 39292.0 41 40286.5 42 41187.0 43 42920.5 44 43594.0 45 43708.5 46 46000.0 47 57873.0 48 86186.5 49 84443.5 50 55769.0 51 41844.0 52 33040.0 53 27006.5 54 23275.0 55 20351.5 56 17515.5 57 14432.5 58 11792.5 59 9779.0 60 7915.5 61 6132.0 62 4391.0 63 2936.0 64 1957.5 65 1276.5 66 776.5 67 454.0 68 242.0 69 143.5 70 92.0 71 53.0 72 33.0 73 18.0 74 9.0 75 7.0 76 5.5 77 2.0 78 1.5 79 1.5 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05323486866889868 2 0.003571668504942084 3 0.03911827410174663 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0016157547998547522 16-17 0.0 18-19 0.0 20-21 0.010927604830596613 22-23 0.0 24-25 0.0 26-27 0.006037820567878284 28-29 0.013606356209303175 30-31 0.010970124693750687 32-33 0.005655141799491632 34-35 0.005655141799491632 36-37 0.02070717335603327 38-39 0.045368693985395274 40-41 0.005017343852180546 42-43 0.01262839935675951 44-45 0.011735482230523989 46-47 0.005144903441642763 48-49 0.00140315548408439 50-51 0.0021685130208576936 52-53 8.503972630814485E-4 54-55 9.354369893895933E-4 56-57 0.01016224729382331 58-59 0.00807877399927376 60-61 0.004294506178561315 62-63 0.003996867136482808 64-65 0.0730916447618505 66-67 0.03248517544971133 68-69 0.02364104391366427 70-71 0.014584313061846843 72-73 8.503972630814485E-5 74-75 2.5511917892443456E-4 76-77 3.826787683866518E-4 78-79 3.826787683866518E-4 80-81 5.102383578488691E-4 82-83 4.677184946947966E-4 84-85 7.653575367733036E-4 86-87 0.0036567082312502284 88-89 1.2755958946221728E-4 90-91 8.503972630814485E-4 92-93 3.401589052325794E-4 94-95 0.001700794526162897 96-97 0.00403938699963688 98-99 0.0055701020731834874 100-101 0.00718585687303824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1175921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.52741525114769 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28644650339669 51.24399778806985 2 11.085378897962416 13.64109421342487 3 2.3767766901883065 4.387107791293929 4 1.0015718653935723 2.464965122637477 5 0.5378821745388276 1.6547249954520373 6 0.33265591031869784 1.2280474997956239 7 0.22332761365774514 0.9618539575797577 8 0.17496490085774363 0.8612110487560223 9 0.11273401026413497 0.6242609036203719 >10 0.7861087871003982 8.740976513842991 >50 0.05296785704486887 2.1929487160152976 >100 0.02318026846807303 2.5549982699427174 >500 0.0020946002820601667 0.8744621494355321 >1k 0.003351360451296267 4.914102682168863 >5k 4.189200564120334E-4 1.7164477128535138 >10k+ 1.3964001880401115E-4 1.938800635111242 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22566 1.9190064638695967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8707 0.7404408969650172 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5996 0.5098981989436365 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5275 0.4485845562754641 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4811 0.40912612326848485 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 0.36218419434638893 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4131 0.3512991093789464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3619 0.3077587695091762 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3578 0.30427214073054226 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3526 0.29985007496251875 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3458 0.2940673735735649 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3397 0.28887995026876806 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3162 0.268895614586354 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2239 0.19040394720393633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2137 0.18172989512050555 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2094 0.17807318688925533 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1756 0.14932975939710236 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1670 0.14201634293460189 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1635 0.13903995251381682 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1610 0.1369139593561132 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1531 0.13019582097776977 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1491 0.12679423192544398 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1414 0.12024617299971681 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1380 0.1173548223052399 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1236 0.10510910171686705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.700794526162897E-4 0.0 10-11 0.0 0.0 0.0 2.5511917892443456E-4 0.0 12-13 0.0 0.0 0.0 2.9763904207850695E-4 0.0 14-15 0.0 0.0 0.0 3.8267876838665184E-4 0.0 16-17 0.0 0.0 0.0 5.102383578488691E-4 0.0 18-19 0.0 0.0 0.0 6.377979473110864E-4 0.0 20-21 0.0 0.0 0.0 9.779568525436657E-4 0.0 22-23 0.0 0.0 0.0 0.0015732349367006799 0.0 24-25 0.0 4.2519863154072425E-5 0.0 0.0022535527471658386 0.0 26-27 0.0 8.503972630814485E-5 0.0 0.0029338705576309972 0.0 28-29 0.0 8.503972630814485E-5 0.0 0.004677184946947967 0.0 30-31 0.0 8.503972630814485E-5 0.0 0.009949647978052947 0.0 32-33 0.0 8.503972630814485E-5 0.0 0.016285107588009738 0.0 34-35 0.0 8.503972630814485E-5 0.0 0.02104733226126585 0.0 36-37 0.0 8.503972630814485E-5 0.0 0.025596957618751602 0.0 38-39 0.0 8.503972630814485E-5 0.0 0.03520644669157197 0.0 40-41 0.0 8.503972630814485E-5 0.0 0.05047107756388397 0.0 42-43 0.0 8.503972630814485E-5 0.0 0.06360971527849235 0.0 44-45 0.0 8.503972630814485E-5 0.0 0.06760658241497516 0.0 46-47 0.0 8.503972630814485E-5 0.0 0.07364440298285344 0.0 48-49 0.0 8.503972630814485E-5 0.0 0.08384917013983081 0.0 50-51 0.0 1.700794526162897E-4 0.0 0.09673268867551477 0.0 52-53 0.0 1.700794526162897E-4 0.0 0.12522099698874328 0.0 54-55 0.0 1.700794526162897E-4 0.0 0.13670136004034283 0.0 56-57 0.0 1.700794526162897E-4 0.0 0.16888889644797567 0.0 58-59 0.0 1.700794526162897E-4 0.0 0.24593488848315492 0.0 60-61 0.0 1.700794526162897E-4 0.0 0.29925479687836176 0.0 62-63 0.0 1.700794526162897E-4 0.0 0.325829711349657 0.0 64-65 0.0 1.700794526162897E-4 0.0 0.4025780643427577 0.0 66-67 0.0 1.700794526162897E-4 0.0 0.6033568581562877 0.0 68-69 0.0 1.700794526162897E-4 0.0 0.8187624848948185 0.0 70-71 0.0 1.700794526162897E-4 0.0 0.9518496565670653 0.0 72-73 0.0 5.102383578488691E-4 0.0 1.1023274522693276 0.0 74-75 0.0 5.102383578488691E-4 0.0 1.2826116720425946 0.0 76-77 0.0 5.102383578488691E-4 0.0 1.3535348037835875 0.0 78-79 0.0 5.102383578488691E-4 0.0 1.4673604774470395 0.0 80-81 0.0 5.102383578488691E-4 0.0 1.6365044930739394 0.0 82-83 0.0 5.102383578488691E-4 0.0 1.7680609496726396 0.0 84-85 0.0 5.102383578488691E-4 0.0 2.0557928636362477 0.0 86-87 0.0 5.102383578488691E-4 0.0 2.7066869287987885 0.0 88-89 0.0 5.102383578488691E-4 0.0 3.5291911616511653 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 300 0.0 63.311222 4 TATAACG 285 0.0 60.002014 2 ATAACGC 350 0.0 54.28292 3 TACCTGG 1445 0.0 48.652153 2 GTACCTG 1590 0.0 43.94274 1 ACCTGGG 1530 0.0 43.77226 3 TCACGCA 210 0.0 42.961185 4 GTACACG 175 0.0 40.739914 1 GTATAAC 490 0.0 39.769917 1 TATCACG 235 0.0 36.3842 2 TACGCAG 315 0.0 36.17784 4 CCTGGGG 1025 0.0 34.743965 4 GAGTACG 380 0.0 32.52046 1 TACACGG 205 1.8189894E-12 30.122963 2 GTACGCA 385 0.0 29.608864 3 CACGCAG 325 0.0 29.220564 5 ACACGGG 245 0.0 29.080135 3 AGTACGC 410 0.0 27.805813 2 ACGCAGT 410 0.0 27.795172 5 CCGGTCG 185 9.002179E-9 25.66671 9 >>END_MODULE