FastQCFastQC Report
Wed 25 May 2016
SRR1003771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35633199
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC5665411.5899246093509594TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA647400.042.5794316
ACGTCTG653700.042.29992715
CACACGT657450.042.0717612
ACACGTC660000.041.97047813
GATCTCG658150.041.898739
ATCTCGT667800.041.24231740
CTCGTAT668850.041.1247342
TCGGAAG684150.041.004663
GTATGCC664250.040.94806745
TCTCGTA673150.040.8453541
TCCAGTC675950.040.7872125
CAGTCAC682750.040.47193527
CGTATGC679200.040.40218444
GATCGGA699250.040.176271
TCGTATG686700.040.0459343
CGGAAGA700800.039.9784554
CTCCAGT691300.039.92685324
TGATCTC688550.039.80816738
GCACACG696850.039.72208411
TCTGAAC696850.039.6702318
Produced by FastQC (version 0.11.5)