FastQCFastQC Report
Wed 25 May 2016
SRR1003770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39675173
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2528990.6374238116113571TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA318050.039.4081116
ACGTCTG325500.038.56146615
ACACGTC328350.038.25394413
ATCTCGT321550.038.08667840
CTCGTAT327500.037.14248742
CACACGT341850.036.8817712
TCGGAAG345900.036.8811343
CACGTCT341850.036.7303214
CGTATGC329750.036.5553844
TCGTATG332150.036.55491343
GTATGCC330400.036.203545
GATCGGA353100.036.1616061
ACTGACC343150.036.142432
AGTCACT351950.035.78322628
TGACCAA345200.035.665134
TCTCGTA345000.035.49923341
GTCACTG354750.035.35275729
CGGAAGA360100.035.2949374
ATCGGAA369350.034.5028842
TCCAGTC364950.034.3646825
Produced by FastQC (version 0.11.5)