FastQCFastQC Report
Wed 25 May 2016
SRR1003769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51367166
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC11941502.3247340528772793TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1344550.042.57713313
CACGTCT1352550.042.30898714
TCGGAAG1372750.042.246033
ACGTCTG1354050.042.24383515
CGTCTGA1357500.042.1083316
CGTATGC1345700.042.02204544
GATCGGA1384550.041.948721
GCACACG1371950.041.82085411
TCTCGTA1361300.041.78471441
CACACGT1372750.041.71449712
ACTTAGG1359600.041.68834732
GTATGCC1350000.041.53778545
TCACTTA1375700.041.44264230
TTAGGCA1365550.041.3316134
TCGTATG1374800.041.29589543
ATCTCGT1383150.041.21290640
CTTAGGC1373250.041.14008333
CACTTAG1390650.041.0748131
TCCAGTC1388600.041.01568625
CTCGTAT1387600.040.9715942
Produced by FastQC (version 0.11.5)