FastQCFastQC Report
Wed 25 May 2016
SRR1003768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80442448
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT2792360.3471251894273531No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGC2545590.3164485993762895No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC1858830.23107576238853397No Hit
CTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC1244670.15472801126092034No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT1197280.14883684295634564No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCT1089510.13543968726560882No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGA916280.11390503680345482No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT878810.10924704827481133No Hit
GCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCT863720.10737117299065788No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTT856900.10652336189470514No Hit
AGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCT848020.10541946709528283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAG335950.022.009721
ACGACCG460950.018.78204517
ACGTCAG483250.017.9631430
GCACGTC478950.017.960128
CACGTCA481750.017.76714129
CGGACGA500750.017.34491314
TTGCACG507200.017.26358226
TGCACGT503500.017.23179627
TCGGACG498850.017.10786413
CGCTACG412550.017.08705740
TGTTTGA2132850.016.7746412
CTGTCGA66650.016.7575729
TACCGCC205300.016.74408534
CGTCAGG505250.016.72287631
GTGTTTG2158850.016.6482941
TTTGAGG1057150.016.5582472
CTAATCG253650.016.54418419
CCGATTT531700.016.49801421
TTGAGGG664150.016.4877973
AGGTCGG578500.016.38932210
Produced by FastQC (version 0.11.5)