FastQCFastQC Report
Wed 25 May 2016
SRR1003767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99125829
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT2272280.2292318786055247No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGC1992860.20104346365668224No Hit
ATCCTGTACTGTCGAGAAAAGCTTCGTGAGCGAGGTGGCGGGTCCGCCCGT1548550.15622063549148224No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC1543130.15567385570112105No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT1126890.11368278191146326No Hit
CTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC1062180.10715471544757522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCG396450.023.09396240
GGTCCGC423600.021.66187541
GTTTAAG402300.020.7450081
CTGTCGA719050.020.6065049
CGAGGTG484700.020.56712731
ACTGTCG650700.020.2051338
CTTCGTG550550.019.80661222
CTGTACT639750.019.7139954
TTGAGGG616400.019.6110423
AGCGAGG560700.019.5022829
TTTGAGG1151850.019.4343242
TGTACTG671800.019.4113965
TGTCGAG772700.019.16413310
GGCGGGT506700.018.75730537
TCGAGAA781800.018.74508312
GTACTGT729150.018.6481656
CGTGAGC584950.018.43764925
GTGTTTG2621200.018.4115721
GCGAGGT547600.018.18827430
TTCGTGA595450.018.18405723
Produced by FastQC (version 0.11.5)