FastQCFastQC Report
Wed 25 May 2016
SRR1003766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47338271
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGC1729110.3652668260739815No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT1543650.3260892228193125No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC1035230.21868775055176815No Hit
CTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC644570.13616255650739756No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT610830.12903513100425656No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCT600340.12681916498386686No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT585080.12359555759862881No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC516240.109053412618302No Hit
AGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCT489160.10333288260570396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAG227850.023.718851
ACGACCG230600.017.71310417
ACGTCAG235550.017.65918430
CGCTACG206300.017.64309740
GCACGTC233250.017.6012728
CACGTCA236300.017.4406529
CTGTCGA38050.017.3102479
TGTTTGA1297850.016.6520792
CGTCAGG244200.016.56956931
ACTGTCG44550.016.5507228
GTGTTTG1311900.016.4779031
TTGCACG251550.016.46568326
CGAACCA131700.016.26955624
TGCACGT254650.016.20188327
CTAATCG132800.016.19966519
TTTGAGG633200.016.158272
GCTACGG225050.016.03336541
ATAGCCG38650.015.99344111
TTGAGGG397450.015.9801683
CGGACGA261350.015.75748414
Produced by FastQC (version 0.11.5)