FastQCFastQC Report
Wed 25 May 2016
SRR1003765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54659828
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGC1308610.2394098276342911No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT1306300.2389872137907203No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC862510.1577959594018481No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT560370.10251953226051132No Hit
CTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC559350.10233292355036316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAG210450.023.7028121
GGGTCCG78450.020.82504340
GGTCCGC78850.020.7193641
CTGTCGA129750.019.109699
ACTGTCG121750.019.0359958
TTGAGGG407950.017.326833
TACCGCC102400.017.27590634
TTTGAGG707450.016.7064532
GCACGTC170150.016.6852628
GTTTGAG1362900.016.127581
TAACCGG69050.016.11493714
GTGTTTG1530000.016.0103171
TGTTTGA1548250.015.9690392
ACGACCG180150.015.92278417
CACGTCA180500.015.85319429
TTGCACG182800.015.82829226
TCGGACG180400.015.74917813
TCGAGAA156950.015.7249612
TGTCGAG162800.015.68545310
TAATACT132950.015.6422824
Produced by FastQC (version 0.11.5)