FastQCFastQC Report
Wed 25 May 2016
SRR1003764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45844818
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1951850.4257514993297607No Hit
GTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTC1357880.2961905094704488No Hit
GTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1011980.220740324457172No Hit
CTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTG997430.2175665742636387No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG814830.17773655465269816No Hit
GACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGAC762430.16630669141275684No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT651420.1420923952626445No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC589470.12857941763450778No Hit
CAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAG569450.12421251186993479No Hit
GTGTTTGGCTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTG486900.10620611472380585No Hit
GTGTTGGGTGTGTTTGGCTGACTGACTGACTGACTGACTGACTGACTGACT486000.10600980027884505No Hit
GGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469250.10235617033096303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGG3092300.037.4379461
TGTTGGG3469800.035.2534262
ATGTTGG222900.031.6436481
AGGTGTG173150.030.0088651
ATGTTTG133100.028.822241
GTTGGGT4755800.024.8057463
TGGGTGT4897300.023.7384765
TTGGGTG5038050.023.4377964
ATTTGGG68650.021.5129281
GGGTGTG6040300.019.2473556
ATTGGGT140650.018.0713441
ATTTGGA57700.017.9090711
GGTGTGT6751850.017.2360297
TGTGTTT6859850.016.853929
GTGTGTT7006500.016.5885128
GAGTAAC78350.015.6026041
CTCGTCA344950.015.49356321
TCGTCAT355100.015.08874122
TAAGGTA355700.015.0561735
ACGAGAT192500.015.03965727
Produced by FastQC (version 0.11.5)