FastQCFastQC Report
Wed 25 May 2016
SRR1003763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52639349
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3454870.6563284055811557No Hit
GTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1421630.2700698293210275No Hit
GGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT764190.14517466771863002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGG3532850.038.9175641
TGTTGGG4013600.036.044252
GTTGGGT5352650.026.0737063
TGGGTGT5548650.024.7889445
TTGGGTG5709800.024.4791534
AGGTGTG298050.023.394641
ATGTTGG355700.023.077941
GGGTGTG6868300.020.0274876
ATTGGGT108400.019.8768221
GGTGTGT7587000.018.1609157
ATGTTTG208800.017.9211061
TGTGTTT7848550.017.4443689
GTGTGTT7934100.017.3417058
CGCGCAT138800.016.52800240
TGGTTTT2859550.016.17206615
ACGCGCA144400.016.1579539
GACGCGC145850.016.10727538
TTGGTTT3011650.016.09157214
GGTTTTT2891850.015.69417616
TTTGGTT3619650.015.27939313
Produced by FastQC (version 0.11.5)