FastQCFastQC Report
Wed 25 May 2016
SRR1003762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46434776
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1991210.4288186939891774No Hit
GTTGGGTGTGTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT859970.18519955819319556No Hit
GTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTC725160.15616743795641438No Hit
CTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTG588400.1267153738396412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGG3013850.037.2498661
TGTTGGG3527800.035.0573962
ATGTTGG400550.029.9910871
AGGTGTG281100.028.9120881
ATTGGGT139200.028.1735781
GACCGTA25150.027.9278224
ATGTTTG209050.026.7722021
GTTGGGT4679550.025.3014683
ACCGTAG28850.024.57965725
TGGGTGT4898750.023.772495
TTGGGTG5028100.023.5676424
TATGCCG31850.021.35567741
ATGCCGT31050.021.18300242
TATCTCG35400.020.28158434
GGGTGTG5919500.019.6518846
CGTAGCT39450.018.37456927
TGCCGTC37450.018.22230343
ATTTGGG125900.017.6491811
GGTGTGT6605400.017.6154127
TGTGTTT6732200.017.183879
Produced by FastQC (version 0.11.5)