FastQCFastQC Report
Wed 25 May 2016
SRR1003761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28326201
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCTT752739026.573948267895155Illumina PCR Primer Index 11 (95% over 24bp)
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGT9651563.4072906564491303No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATG8813363.111380873135794No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTC7999672.824123856213546Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCC6382532.253224850024894No Hit
GAGATGTGTATAAGAGACAGACACATCTCTGAGCGGGCTGGCAAGGCAGAC1423100.5023970563507616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1133510.4001630857593646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1021020.3604507360517564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT744910.26297561046043555No Hit
GAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCTTCTGCT692230.2443779877153311Illumina PCR Primer Index 11 (96% over 29bp)
ACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGT685470.24199150461440277Illumina PCR Primer Index 11 (95% over 21bp)
ATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCT677780.2392767035720745Illumina PCR Primer Index 11 (95% over 23bp)
CTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCTTC658710.23254442062315384Illumina PCR Primer Index 11 (96% over 25bp)
TATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTAT516660.1823965027996518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504300.17803305144943368No Hit
CACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCG493320.17415678156064768No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGGCTT442960.15637818851882043No Hit
TTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTA422340.1490987090008999No Hit
TCTGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCTTCT386970.13661203632636795Illumina PCR Primer Index 11 (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377040.13310644798432378No Hit
TCTCCGAGCGGGCTGGCAAGGCAGACCGGGCTACATCTCGTATGCCGTCTT322310.11378511364796148Illumina PCR Primer Index 11 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT8514150.043.0790345
GCCGTCT8607050.042.5333844
CCGTCGT9850.040.57177445
CTTATAC1276250.039.261321
TGCCGTC9487050.038.63785643
ATGCCGT9576000.038.2827942
TATGCCG9692300.038.02087441
TTATACA1341500.037.4728132
TATACAC1350950.037.206833
GACACAT198850.036.7835220
CAGACAC201150.036.28468318
GTATGCC10445200.035.26539640
CGTATGC10445600.035.26377539
TGCCGGC88000.034.90041443
ATGCCGG88300.034.3736442
GCCGGCT88950.034.27624544
GCCGTCC13300.034.09880444
CCGGCTT92200.033.603845
GACAGAC216400.033.57319616
GCCGTCG12250.033.3560644
Produced by FastQC (version 0.11.5)