FastQCFastQC Report
Wed 25 May 2016
SRR1003758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34639257
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367950.10622341004600648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA559650.040.4789241
TCAACGC574500.037.7745064
CAACGCA586700.036.9230965
ATCAACG596000.036.3225173
AACGCAG621450.034.920576
GGTATCA327750.031.9192771
ACGCAGA710600.030.4527447
TATCAAC767350.028.7801842
CGCAGAG771100.028.0820228
GTACATG983850.026.2987881
TACATGG1009600.024.7444172
GCAGAGT930300.023.062269
ACATGGG1061200.022.8352153
CATGGGG729550.019.5275464
AGAGTAC851900.019.4355710-11
GAGTACA691850.017.2391681
CAGAGTA922000.016.9829410-11
GTATATA204150.015.7728671
GTATTAG185400.015.5263431
CGCGCGT19300.015.2380977
Produced by FastQC (version 0.11.5)