##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003758_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34639257 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36806167638065 34.0 31.0 34.0 31.0 34.0 2 32.487307565517355 34.0 31.0 34.0 31.0 34.0 3 32.48223938521545 34.0 31.0 34.0 31.0 34.0 4 35.578469826878795 37.0 35.0 37.0 33.0 37.0 5 35.59821358177515 37.0 35.0 37.0 33.0 37.0 6 35.92594985510226 37.0 37.0 37.0 35.0 37.0 7 35.9165665995665 37.0 37.0 37.0 35.0 37.0 8 35.91445024354881 37.0 37.0 37.0 35.0 37.0 9 37.558315988128726 39.0 38.0 39.0 35.0 39.0 10-11 37.49238111544945 39.0 37.5 39.0 35.0 39.0 12-13 37.498713482797854 39.0 37.5 39.0 35.0 39.0 14-15 38.919982738082396 41.0 38.0 41.0 36.0 41.0 16-17 38.899212142454445 41.0 38.0 41.0 36.0 41.0 18-19 38.853685328758644 41.0 38.0 41.0 36.0 41.0 20-21 38.80260780420319 41.0 38.0 41.0 36.0 41.0 22-23 38.72296734020594 40.0 38.5 41.0 36.0 41.0 24-25 38.66400937814572 40.0 39.0 41.0 35.0 41.0 26-27 38.57980993934137 40.0 38.5 41.0 35.0 41.0 28-29 38.45436007764255 40.0 38.0 41.0 35.0 41.0 30-31 38.22562940654299 40.0 38.0 41.0 34.0 41.0 32-33 38.01311576919794 40.0 38.0 41.0 34.0 41.0 34-35 38.00119982365673 40.0 38.0 41.0 34.0 41.0 36-37 38.043583267389366 40.0 38.0 41.0 34.0 41.0 38-39 38.03549324109348 40.0 38.0 41.0 34.0 41.0 40-41 37.94904897931269 40.0 38.0 41.0 34.0 41.0 42-43 37.81938131640641 40.0 38.0 41.0 33.0 41.0 44-45 37.6394236314018 40.0 38.0 41.0 33.0 41.0 46-47 37.478619936911464 40.0 38.0 41.0 33.0 41.0 48-49 37.25361340747003 40.0 37.5 41.0 32.5 41.0 50-51 36.91553444694267 39.5 37.5 40.5 31.5 41.0 52-53 37.185529903253986 39.5 37.5 40.5 33.0 41.0 54-55 37.463798703880975 40.0 38.0 41.0 33.0 41.0 56-57 37.339701714733664 40.0 37.0 41.0 33.0 41.0 58-59 37.16116952219847 40.0 37.0 41.0 32.5 41.0 60-61 36.93789061353135 39.0 36.5 41.0 32.0 41.0 62-63 36.69277715743152 39.0 36.0 41.0 31.5 41.0 64-65 36.409679024004475 39.0 35.5 41.0 31.0 41.0 66-67 36.11808946999065 39.0 35.0 40.5 31.0 41.0 68-69 35.77405407396584 38.0 35.0 40.0 30.5 41.0 70-71 35.441849113565 37.0 35.0 39.5 30.0 41.0 72-73 35.074485272591154 37.0 35.0 39.0 30.0 41.0 74-75 34.67111536197211 36.5 35.0 39.0 29.0 40.5 76-77 34.28339275579728 36.0 34.5 39.0 29.0 39.5 78-79 33.88074136520885 35.5 34.0 37.5 29.0 39.0 80-81 33.50324516198485 35.0 34.0 37.0 28.0 39.0 82-83 33.143685082506245 35.0 34.0 37.0 27.5 39.0 84-85 32.83276613005874 35.0 34.0 36.0 26.5 38.5 86-87 32.54802978308686 35.0 34.0 36.0 26.0 37.0 88-89 32.284136449578 35.0 33.5 36.0 26.0 37.0 90-91 32.00649130551501 35.0 33.0 35.0 25.0 37.0 92-93 31.738235378432048 35.0 33.0 35.0 24.5 36.5 94-95 31.490143177724626 35.0 33.0 35.0 23.5 36.0 96-97 31.253001341801298 35.0 33.0 35.0 20.5 36.0 98-99 30.948569552170245 35.0 32.5 35.0 18.0 36.0 100-101 30.009982849805354 34.5 31.0 35.0 2.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5733544685944629 1101 2 -0.5348782391633264 1101 3 -0.4538082751144046 1101 4 -1.1305535209523043 1101 5 -1.089717588827476 1101 6 -0.4383296513135875 1101 7 -0.3991340785615449 1101 8 -0.4585661480258665 1101 9 -0.8539730418829024 1101 10-11 -0.780573907243884 1101 12-13 -0.6752886042985295 1101 14-15 -0.8294408608000126 1101 16-17 -0.8361627381430381 1101 18-19 -0.8184535157176498 1101 20-21 -0.8014616125567642 1101 22-23 -0.7688422898185223 1101 24-25 -0.6357058611943316 1101 26-27 -0.6885240572332734 1101 28-29 -0.6414492213680845 1101 30-31 -0.517021972795142 1101 32-33 -0.5623969154431094 1101 34-35 -0.4818950532721047 1101 36-37 -0.599896089463769 1101 38-39 -0.6214851377029262 1101 40-41 -0.6892093440525713 1101 42-43 -0.6886270633899514 1101 44-45 -0.6104255824800404 1101 46-47 -0.6402264686790318 1101 48-49 -0.5052946758414762 1101 50-51 -0.4487193757986532 1101 52-53 -0.4968865841513903 1101 54-55 -0.5834670105598931 1101 56-57 -0.6061196156460369 1101 58-59 -0.5555783211936642 1101 60-61 -0.4909224961772267 1101 62-63 -0.434880818430571 1101 64-65 -0.45890587655127746 1101 66-67 -0.3756373912686968 1101 68-69 -0.2893662260476546 1101 70-71 -0.24835350096191178 1101 72-73 -0.11898009198731074 1101 74-75 -0.04444611452588987 1101 76-77 0.09756407623977736 1101 78-79 0.11608804967503517 1101 80-81 0.275080142721265 1101 82-83 0.36706030849986604 1101 84-85 0.46441024430949795 1101 86-87 0.4192416951224587 1101 88-89 0.5695141784689994 1101 90-91 0.6338141397192167 1101 92-93 0.6398271440637409 1101 94-95 0.6731580939931483 1101 96-97 0.735039888149668 1101 98-99 0.7962456315178983 1101 100-101 0.8521741417523145 1102 1 -0.4970595441024557 1102 2 -0.348184879642659 1102 3 -0.2629757269831998 1102 4 -1.2625578735599774 1102 5 -1.2720712800329537 1102 6 -0.43029581593309985 1102 7 -0.36940268519986574 1102 8 -0.4201449500115473 1102 9 -0.8906237979594138 1102 10-11 -0.8508793548910702 1102 12-13 -0.6268700833653753 1102 14-15 -0.7364486471600955 1102 16-17 -0.8145068211909035 1102 18-19 -0.7583634412994584 1102 20-21 -0.7201655452219455 1102 22-23 -0.5758315592317444 1102 24-25 -0.5294607094357318 1102 26-27 -0.47019382291674106 1102 28-29 -0.4396435538321555 1102 30-31 -0.26133065375521625 1102 32-33 -0.27084260420923556 1102 34-35 -0.23172832841850521 1102 36-37 -0.3163396486202714 1102 38-39 -0.35769485450294525 1102 40-41 -0.30735850569459444 1102 42-43 -0.3074973532219616 1102 44-45 -0.298538474132755 1102 46-47 -0.2600558784931408 1102 48-49 -0.1318798378036803 1102 50-51 -0.09658307346899164 1102 52-53 -0.13101392601473805 1102 54-55 -0.21975981653314847 1102 56-57 -0.14274589945553373 1102 58-59 -0.05839148664644256 1102 60-61 -0.025306192069812994 1102 62-63 0.044558036907062615 1102 64-65 0.11676835047871492 1102 66-67 0.2159187242423357 1102 68-69 0.36961436057307395 1102 70-71 0.3878350065400298 1102 72-73 0.5331217984772323 1102 74-75 0.6778952408889083 1102 76-77 0.7805094518091096 1102 78-79 0.9091430151904376 1102 80-81 1.0466291592656418 1102 82-83 1.132339321276561 1102 84-85 1.2518659732149686 1102 86-87 1.2656801886402391 1102 88-89 1.3749472789260082 1102 90-91 1.4699820668080967 1102 92-93 1.5417719639639351 1102 94-95 1.6040572123164445 1102 96-97 1.7221438272122036 1102 98-99 1.7636503500120853 1102 100-101 1.9630724399130912 1103 1 -0.46741967665162676 1103 2 -0.38094979327143363 1103 3 -0.24818003667834887 1103 4 -0.9663858404005197 1103 5 -0.9621570601816103 1103 6 -0.3630497392112133 1103 7 -0.27546732693291176 1103 8 -0.43375072705704554 1103 9 -0.8371620222142866 1103 10-11 -0.7577087033756982 1103 12-13 -0.6517462341197344 1103 14-15 -0.7382029934281746 1103 16-17 -0.7833475146727906 1103 18-19 -0.7397531295798672 1103 20-21 -0.7444803568898237 1103 22-23 -0.651391282090195 1103 24-25 -0.6389215080555672 1103 26-27 -0.5607468776800886 1103 28-29 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0.7389519692836295 1103 94-95 0.7638760285356163 1103 96-97 0.7942912309034789 1103 98-99 0.8557649581275797 1103 100-101 0.924598500897531 1104 1 -0.4354949553201273 1104 2 -0.3077430112135531 1104 3 -0.22420429625393012 1104 4 -0.9283673748568901 1104 5 -0.9213632214024656 1104 6 -0.30157190572460024 1104 7 -0.27341204670896246 1104 8 -0.3227304843171481 1104 9 -0.8087397523845041 1104 10-11 -0.72066701904685 1104 12-13 -0.5674237948084837 1104 14-15 -0.6515361662724573 1104 16-17 -0.7352227129794429 1104 18-19 -0.6685611880662705 1104 20-21 -0.6725035160049515 1104 22-23 -0.6675652557438383 1104 24-25 -0.5885091444230781 1104 26-27 -0.6588231041977721 1104 28-29 -0.4946200396040652 1104 30-31 -0.3589404449714877 1104 32-33 -0.3036043271499125 1104 34-35 -0.23591219342085878 1104 36-37 -0.37260346251649423 1104 38-39 -0.39476721188474784 1104 40-41 -0.34149784503127023 1104 42-43 -0.3164396155021194 1104 44-45 -0.3816435845944781 1104 46-47 -0.3672773432123506 1104 48-49 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-0.26363753647451915 1105 8 -0.3940787315019634 1105 9 -0.7002886632443364 1105 10-11 -0.6610154699847257 1105 12-13 -0.5965154887345463 1105 14-15 -0.6198991899277146 1105 16-17 -0.7243067993142915 1105 18-19 -0.6631905050731888 1105 20-21 -0.6585321602658496 1105 22-23 -0.5690120277705688 1105 24-25 -0.5671198154561594 1105 26-27 -0.5270835062688022 1105 28-29 -0.4659601569692384 1105 30-31 -0.4123162701788843 1105 32-33 -0.42723578192217104 1105 34-35 -0.29527022240667833 1105 36-37 -0.35587824316425554 1105 38-39 -0.41153109237061614 1105 40-41 -0.396881381335632 1105 42-43 -0.40938347313826284 1105 44-45 -0.43267822038331616 1105 46-47 -0.37588322163716725 1105 48-49 -0.29953289816685214 1105 50-51 -0.2616404456854866 1105 52-53 -0.29649583017238257 1105 54-55 -0.29598674040125417 1105 56-57 -0.28827906699024197 1105 58-59 -0.23878198152758756 1105 60-61 -0.22602973068141097 1105 62-63 -0.17934465740253813 1105 64-65 -0.1360061539688573 1105 66-67 8.319565758796443E-5 1105 68-69 0.011840166860444867 1105 70-71 0.11622473320137772 1105 72-73 0.116236356319952 1105 74-75 0.2776239446732376 1105 76-77 0.26818487458680806 1105 78-79 0.39155205615237065 1105 80-81 0.4759668438736 1105 82-83 0.5429927386303106 1105 84-85 0.6266257396687038 1105 86-87 0.6483112218103528 1105 88-89 0.6756844116625373 1105 90-91 0.7828947934592101 1105 92-93 0.7607725887285461 1105 94-95 0.8368418756341462 1105 96-97 0.741082278361727 1105 98-99 0.9071836755346148 1105 100-101 0.8396626397296671 1106 1 -0.42693341638733173 1106 2 -0.2195569149514398 1106 3 -0.14178411147294412 1106 4 -0.7503782377520949 1106 5 -0.7710028719517865 1106 6 -0.20117555570957535 1106 7 -0.3843030441587061 1106 8 -0.3445300966034992 1106 9 -0.617229237230525 1106 10-11 -0.6430158477789547 1106 12-13 -0.5301818910392626 1106 14-15 -0.5687823792319833 1106 16-17 -0.6309847945828935 1106 18-19 -0.5901667649014399 1106 20-21 -0.5711602881862987 1106 22-23 -0.5376126836524335 1106 24-25 -0.43865048658786776 1106 26-27 -0.4329523969206335 1106 28-29 -0.3663097983459025 1106 30-31 -0.23250696229974466 1106 32-33 -0.27155801420217784 1106 34-35 -0.14988393704216918 1106 36-37 -0.23615136037337692 1106 38-39 -0.2290050565602897 1106 40-41 -0.24254644983645335 1106 42-43 -0.2300195681263304 1106 44-45 -0.2685077110824423 1106 46-47 -0.26790655577517697 1106 48-49 -0.19967108365940334 1106 50-51 -0.10992560603718715 1106 52-53 -0.214480776141329 1106 54-55 -0.20667500972388808 1106 56-57 -0.19805276473557143 1106 58-59 -0.12544860253429135 1106 60-61 -0.13457343641899655 1106 62-63 -0.07399535153007264 1106 64-65 -0.009188854391730672 1106 66-67 -0.006814589487561307 1106 68-69 0.11945237032322353 1106 70-71 0.22453174711222346 1106 72-73 0.2984020975666013 1106 74-75 0.35726935468824905 1106 76-77 0.40283162661863514 1106 78-79 0.554249727910566 1106 80-81 0.5764853786416992 1106 82-83 0.6438781090245627 1106 84-85 0.7511879606094283 1106 86-87 0.7573833039685454 1106 88-89 0.8063757954311157 1106 90-91 0.8694505361912341 1106 92-93 0.9265222943288016 1106 94-95 0.905558624909677 1106 96-97 0.8839411489053042 1106 98-99 1.0025029416838294 1106 100-101 0.9118129248480216 1107 1 -0.32730094626404593 1107 2 -0.21106424702703208 1107 3 -0.14962779706824847 1107 4 -0.6921405202171513 1107 5 -0.672137057712753 1107 6 -0.20836841211609425 1107 7 -0.27105867302103803 1107 8 -0.22327389615356452 1107 9 -0.5061897450117385 1107 10-11 -0.5545655304487696 1107 12-13 -0.5089464368196488 1107 14-15 -0.578881883624895 1107 16-17 -0.5094565736281425 1107 18-19 -0.603934266510862 1107 20-21 -0.488237557850411 1107 22-23 -0.5219390401452557 1107 24-25 -0.39965304348026365 1107 26-27 -0.3863088005827464 1107 28-29 -0.3823673443381992 1107 30-31 -0.26391966612940365 1107 32-33 -0.289605593105712 1107 34-35 -0.21121470629197603 1107 36-37 -0.2773073463336573 1107 38-39 -0.41113646193856823 1107 40-41 -0.30429708492252416 1107 42-43 -0.2701863729500502 1107 44-45 -0.3743075327681922 1107 46-47 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0.22403806923993486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109259.0 3 24361.0 4 25517.0 5 25342.0 6 27820.0 7 29221.0 8 35793.0 9 42705.0 10 50639.0 11 57672.0 12 65508.0 13 68849.0 14 70707.0 15 78614.0 16 95748.0 17 125500.0 18 114121.0 19 91912.0 20 98403.0 21 110671.0 22 123093.0 23 135766.0 24 151885.0 25 171566.0 26 199544.0 27 233269.0 28 276081.0 29 330964.0 30 404399.0 31 504594.0 32 645229.0 33 859524.0 34 1236183.0 35 1944616.0 36 3588499.0 37 7810188.0 38 1.1411893E7 39 3261491.0 40 2111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53785784038762 20.282252000281936 14.348305349498375 23.83158480983206 2 14.517212417311434 21.27427278251417 40.38687615929589 23.821638640878508 3 16.712462020909065 27.4328238777214 30.976321187924803 24.878392913444728 4 12.065140822062167 17.947010745584894 40.26512495459209 29.722723477760848 5 12.670716630973786 38.442057747833175 36.3607812696857 12.526444351507337 6 28.96855385031235 39.238961106076495 19.10857188243408 12.683913161177074 7 26.02140569400212 33.15177675248603 24.50187546424904 16.32494208926281 8 24.783880842904303 36.077293816515486 21.681506742825228 17.457318597754984 9 25.776478825861226 16.764086615329973 21.314126393998325 36.14530816481048 10-11 23.391267481196792 26.873001818177638 30.1716835522717 19.564047148353872 12-13 24.233353816275155 24.362414570095808 29.89540887589197 21.508822737737066 14-15 21.796150033897455 25.90942218245134 26.768113823863015 25.526313959788183 16-17 21.555932973427247 29.37453023254926 27.23306362520016 21.83647316882333 18-19 21.588518676626887 28.677749267642184 28.80742561373784 20.926306441993084 20-21 22.357543527085117 27.956026086427794 28.4635540192085 21.222876367278584 22-23 22.177252197724652 27.67372447770634 28.406915244361215 21.742108080207796 24-25 21.912071120165624 27.862809228498968 28.446338553041123 21.77878109829428 26-27 21.695439464014605 28.148219600300077 28.348756847723145 21.807584087962166 28-29 21.87882690721217 27.943904019349542 28.474937909706632 21.70233116373166 30-31 21.998062100736423 28.088095547826132 28.21101224893511 21.70283010250234 32-33 21.854900134964687 28.03819015780387 28.326171188580435 21.78073851865101 34-35 22.072092724911478 27.862161638054683 28.436974602577326 21.628771034456516 36-37 22.19111253263127 27.983787033518624 28.121333028755014 21.70376740509509 38-39 21.991541646190484 28.157090479562896 28.17227144163068 21.679096432615943 40-41 21.910833412849044 27.94951980381675 28.34772473552562 21.791922047808583 42-43 21.87513705281794 27.98106564502792 28.360033625750358 21.78376367640378 44-45 21.84497601081144 28.102797390713746 28.233806681723138 21.818419916751676 46-47 21.94646606074943 28.058781864929745 28.14233162210023 21.852420452220592 48-49 22.014752483736622 28.169509937775473 27.96180759417497 21.853929984312938 50-51 22.083109630392254 28.20401575079039 27.84167885626688 21.87119576255047 52-53 21.90601163451417 28.20429359920544 28.021956045133443 21.86773872114695 54-55 21.877213846052037 28.38158053463936 28.02682841059862 21.71437720870998 56-57 21.96816278710919 28.228771246001877 27.984512619486424 21.81855334740251 58-59 21.864255482105143 27.89601871501389 28.152333199306916 22.087392603574045 60-61 22.001170144596436 28.10233301649455 28.089000459880577 21.807496379028432 62-63 21.849418468177294 28.23518296288603 27.99342076178233 21.92197780715435 64-65 21.982441378145197 27.994165351125332 28.055027363592643 21.96836590713683 66-67 21.841201023747598 28.09415583598363 28.065759549737606 21.998883590531165 68-69 21.951602406336875 28.23764964005939 27.90924091119941 21.901507042404322 70-71 21.90908024170361 27.960648680310108 28.102113544530532 22.02815753345575 72-73 21.816802087398237 28.115601452713136 28.03832479964821 22.029271660240422 74-75 21.923606385879477 28.11338180005951 27.94776446025019 22.015247353810825 76-77 21.993875137037637 27.95050273686201 27.93441771445066 22.121204411649696 78-79 21.87169244781817 28.206958333282707 27.987747523442053 21.93360169545707 80-81 21.89385805847115 28.11416419964865 27.960524354701654 22.031453387178544 82-83 21.831416010927214 28.00272638980829 28.074876460914105 22.090981138350386 84-85 21.849860066519557 28.18111422717538 27.938258689313177 22.030767016991888 86-87 21.997630672160547 28.094346672766175 27.883868209416242 22.02415444565704 88-89 21.864779166338753 28.04638276522955 28.03877898579239 22.050059082639308 90-91 21.89001584860381 28.075004198607616 28.051112449351447 21.983867503437125 92-93 21.909032556349263 27.963758414216045 28.02649129703055 22.100717732404142 94-95 21.82238304200299 27.836521730672846 28.20436407598057 22.13673115134359 96-97 21.919436240673114 27.977359877121245 28.053234573825737 22.0499693083799 98-99 21.799860451499733 28.095916677391685 27.98414299737113 22.12007987373745 100-101 21.851290916533685 27.82585485597092 28.075032113513444 22.247822113981954 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32639.0 1 20306.0 2 7347.5 3 6129.5 4 4923.0 5 4442.5 6 5225.0 7 7526.5 8 11150.0 9 12444.5 10 10491.0 11 9481.0 12 10226.5 13 11187.0 14 12077.0 15 12987.0 16 13923.5 17 14806.0 18 15796.5 19 17077.0 20 18932.0 21 21821.0 22 26796.0 23 35855.0 24 49493.5 25 69186.0 26 99025.0 27 144109.5 28 209200.0 29 289443.5 30 374371.0 31 466485.5 32 560200.0 33 651015.0 34 753717.0 35 867001.5 36 986412.0 37 1118638.0 38 1250632.5 39 1363827.0 40 1473776.0 41 1552992.0 42 1580560.0 43 1627103.0 44 1665306.5 45 1653326.5 46 1643321.0 47 1594702.0 48 1503464.0 49 1420869.5 50 1331073.0 51 1219226.0 52 1089516.5 53 962650.5 54 848382.0 55 738674.5 56 625353.0 57 517809.0 58 432159.5 59 370154.5 60 313573.0 61 251440.5 62 193612.0 63 143255.0 64 102659.0 65 73194.5 66 49768.5 67 31323.0 68 18999.5 69 11307.5 70 6481.5 71 3954.0 72 2459.0 73 1624.5 74 1149.0 75 859.5 76 634.0 77 478.0 78 361.5 79 278.0 80 234.0 81 203.5 82 163.5 83 131.5 84 107.0 85 86.0 86 74.5 87 63.5 88 62.5 89 54.5 90 37.0 91 28.0 92 29.0 93 27.0 94 20.5 95 18.5 96 14.5 97 12.0 98 10.0 99 7.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14400713040698304 2 0.10807102473358479 3 0.12295009676448893 4 0.15235026548057887 5 0.16377372066612167 6 0.11835992902503654 7 0.11265830557508782 8 0.10294966777145363 9 0.11274491251356807 10-11 0.17278805951293932 12-13 0.18048308599690807 14-15 0.14117075317175537 16-17 0.1549123874106191 18-19 0.2146596273701829 20-21 0.19903140532142477 22-23 0.15802301995103415 24-25 0.16065009708493458 26-27 0.2189784267023972 28-29 0.23359767791786065 30-31 0.29107437264026764 32-33 0.4711460756793946 34-35 0.546901164768055 36-37 0.45211708784631266 38-39 0.4007259740011167 40-41 0.3702677571865932 42-43 0.40617499388049805 44-45 0.4112876901487812 46-47 0.32139546180219747 48-49 0.5039138108533909 50-51 0.26277266859390197 52-53 0.2523322021601098 54-55 0.23029939701073843 56-57 0.24332219366021623 58-59 0.26010228799076146 60-61 0.26630045788799683 62-63 0.25270894234249885 64-65 0.2592188972182631 66-67 0.2161651446507643 68-69 0.2372077438035117 70-71 0.22422969407224874 72-73 0.2056871485436307 74-75 0.20240330212625518 76-77 0.201593527251465 78-79 0.20938959516366068 80-81 0.24652665038398486 82-83 0.269077653715263 84-85 0.24945973870051544 86-87 0.24095493734175652 88-89 0.2237259303800887 90-91 0.28304302254520064 92-93 0.3069667458513905 94-95 0.2698643334064585 96-97 0.23373480557045434 98-99 0.25975874713478986 100-101 0.3326846762330959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.4639257E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.970137290305168 #Duplication Level Percentage of deduplicated Percentage of total 1 62.55114620646971 19.372175856803185 2 17.17534036171827 10.638452980202688 3 6.786458072705942 6.305326146798085 4 3.508912032702101 4.346859495695514 5 2.1500631428432433 3.329387535834013 6 1.404859354742081 2.6105212253959067 7 0.9669782821656574 2.09632151087897 8 0.7415943081844099 1.8373822030544045 9 0.5763577944041669 1.6064892018931076 >10 3.5827692170082845 20.836828351243255 >50 0.32291680836267855 6.942920903459853 >100 0.20869569100585983 12.360916803581063 >500 0.01658174974903944 3.513700900139522 >1k 0.0071673036424040005 3.680013223522106 >5k 1.2210387404310282E-4 0.23098725033053122 >10k+ 3.757042211830508E-5 0.2917164111677082 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36795 0.10622341004600648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.886897949341119E-6 0.0 0.0 0.0 0.0 2 2.886897949341119E-6 2.886897949341119E-6 0.0 0.0 0.0 3 2.886897949341119E-6 2.886897949341119E-6 0.0 5.773795898682238E-6 0.0 4 2.886897949341119E-6 2.886897949341119E-6 0.0 2.886897949341119E-5 0.0 5 2.886897949341119E-6 8.660693848023357E-6 0.0 2.886897949341119E-5 0.0 6 2.886897949341119E-6 8.660693848023357E-6 0.0 2.886897949341119E-5 0.0 7 2.886897949341119E-6 8.660693848023357E-6 0.0 3.4642775392093426E-5 0.0 8 8.660693848023357E-6 8.660693848023357E-6 0.0 3.752967334143455E-5 0.0 9 1.7321387696046713E-5 8.660693848023357E-6 0.0 7.50593466828691E-5 0.0 10-11 1.7321387696046713E-5 8.660693848023357E-6 0.0 1.4001455054304428E-4 0.0 12-13 1.7321387696046713E-5 8.660693848023357E-6 0.0 1.6888353003645547E-4 0.0 14-15 1.876483667071727E-5 1.0104142822693917E-5 0.0 2.0930010132723113E-4 0.0 16-17 2.0208285645387833E-5 1.1547591797364477E-5 0.0 2.771422031367474E-4 0.0 18-19 2.0208285645387833E-5 1.1547591797364477E-5 0.0 3.651925905916515E-4 0.0 20-21 2.0208285645387833E-5 1.1547591797364477E-5 0.0 5.008767942106841E-4 0.0 22-23 2.0208285645387833E-5 1.7321387696046713E-5 0.0 6.048051203869644E-4 0.0 24-25 2.0208285645387833E-5 2.886897949341119E-5 0.0 7.361589770819854E-4 0.0 26-27 2.0208285645387833E-5 4.1860020265446225E-5 0.0 8.761735276250297E-4 0.0 28-29 2.0208285645387833E-5 4.7633816164128465E-5 0.0 0.0013669461790130196 0.0 30-31 2.0208285645387833E-5 5.196416308814014E-5 0.0 0.003205900172743313 0.0 32-33 2.0208285645387833E-5 5.196416308814014E-5 0.0 0.007298078015934349 0.0 34-35 2.3095183594728953E-5 5.6294510012151814E-5 0.0 0.012683586140430206 0.0 36-37 2.3095183594728953E-5 7.361589770819853E-5 0.0 0.021550693191831453 0.0 38-39 2.742553051874063E-5 8.660693848023357E-5 0.0 0.03791796111562093 0.0 40-41 3.175587744275231E-5 9.238073437891581E-5 0.0 0.05795158943507363 0.0 42-43 3.175587744275231E-5 9.671108130292749E-5 0.0 0.0787213767316083 0.0 44-45 3.175587744275231E-5 1.0104142822693916E-4 0.0 0.10255849309931792 0.0 46-47 3.175587744275231E-5 1.0392832617628028E-4 0.0 0.1309179351046704 0.0 48-49 3.175587744275231E-5 1.0392832617628028E-4 0.0 0.16263339597613194 0.0 50-51 3.175587744275231E-5 1.1547591797364476E-4 0.0 0.19770631916267717 0.0 52-53 3.175587744275231E-5 1.2413661182166812E-4 0.0 0.23491121648481086 0.0 54-55 3.4642775392093426E-5 1.3135385669502093E-4 0.0 0.27439387628897466 0.0 56-57 4.6190367189457906E-5 1.385711015683737E-4 0.0 0.31951320433922703 0.0 58-59 5.629451001215182E-5 1.4145799951771482E-4 0.0 0.3710890796531808 0.0 60-61 6.206830591083406E-5 1.4434489746705594E-4 0.0 0.428639390273296 0.0 62-63 6.78421018095163E-5 1.4723179541639706E-4 0.0 0.48856561790571895 0.0 64-65 7.361589770819854E-5 1.5156214234040877E-4 0.0 0.5504693706334405 0.0 66-67 7.794624463221022E-5 1.6599663208711433E-4 0.0 0.6192728094600874 0.0 68-69 8.516348950556301E-5 1.8476146875783163E-4 0.0 0.6965146509926584 0.0 70-71 8.660693848023357E-5 1.919787136311844E-4 0.0 0.7835142075940024 0.0 72-73 8.660693848023357E-5 1.963090605551961E-4 0.0 0.8765560416033173 0.0 74-75 8.949383642957468E-5 1.963090605551961E-4 0.0 0.971110610138087 0.0 76-77 9.093728540424524E-5 2.0208285645387832E-4 0.0 1.0734352645035083 0.0 78-79 9.238073437891581E-5 2.0496975440321944E-4 0.0 1.18973539184169 0.0 80-81 9.671108130292749E-5 2.0785665235256056E-4 0.0 1.3185747604228346 0.0 82-83 1.0104142822693916E-4 2.1074355030190167E-4 0.0 1.4567243748906047 0.0 84-85 1.0825867310029197E-4 2.1218699927657225E-4 0.0 1.5968226454741798 0.0 86-87 1.1547591797364476E-4 2.1507389722591336E-4 0.0 1.7452092000703132 0.0 88-89 1.255800607963387E-4 2.1651734620058393E-4 0.0 1.9104119352213589 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 55965 0.0 40.478924 1 TCAACGC 57450 0.0 37.774506 4 CAACGCA 58670 0.0 36.923096 5 ATCAACG 59600 0.0 36.322517 3 AACGCAG 62145 0.0 34.92057 6 GGTATCA 32775 0.0 31.919277 1 ACGCAGA 71060 0.0 30.452744 7 TATCAAC 76735 0.0 28.780184 2 CGCAGAG 77110 0.0 28.082022 8 GTACATG 98385 0.0 26.298788 1 TACATGG 100960 0.0 24.744417 2 GCAGAGT 93030 0.0 23.06226 9 ACATGGG 106120 0.0 22.835215 3 CATGGGG 72955 0.0 19.527546 4 AGAGTAC 85190 0.0 19.43557 10-11 GAGTACA 69185 0.0 17.239168 1 CAGAGTA 92200 0.0 16.98294 10-11 GTATATA 20415 0.0 15.772867 1 GTATTAG 18540 0.0 15.526343 1 CGCGCGT 1930 0.0 15.238097 7 >>END_MODULE