FastQCFastQC Report
Wed 25 May 2016
SRR1003758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34639257
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415660.11999680016231294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA604850.042.0144771
TCAACGC651700.037.4818654
CAACGCA663200.036.8533555
ATCAACG677750.036.244653
GGTATCA359500.035.33091
AACGCAG701750.034.8764276
ACGCAGA794100.030.7906937
TATCAAC842600.029.5383052
CGCAGAG847250.028.863358
GTACATG964300.025.482741
GCAGAGT1018500.023.926359
TACATGG997050.023.8868482
ACATGGG1040750.022.4029183
AGAGTAC945650.020.25313610-11
CATGGGG715100.019.2213614
CAGAGTA1013900.017.93419310-11
GAGTACA695450.016.4462051
GAGTACT584050.016.12208412-13
GTATATA199350.015.9824241
AGTACTT657750.015.2038112-13
Produced by FastQC (version 0.11.5)