FastQCFastQC Report
Wed 25 May 2016
SRR1003757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39662302
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528680.1332953392367392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA678800.041.5067831
TCAACGC716300.038.117084
CAACGCA733750.037.1590235
ATCAACG743450.036.6275943
GGTATCA394100.036.0405731
AACGCAG772900.035.339196
ACGCAGA880550.031.0027057
TATCAAC921650.029.9882032
CGCAGAG930250.029.3107178
GTACATG1091500.025.752091
TACATGG1116000.024.3068562
GCAGAGT1117000.024.282899
ACATGGG1164750.022.638053
AGAGTAC1038500.019.68474410-11
CATGGGG773900.019.0280844
CAGAGTA1112850.018.17138710-11
GAGTACA780750.016.9895461
GAGTACT617650.016.71425412-13
GTATTAG211550.015.5353671
GTATAGA192500.015.1018811
Produced by FastQC (version 0.11.5)