FastQCFastQC Report
Wed 25 May 2016
SRR1003757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39662302
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576490.14534960678782588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA737100.041.6941261
GGTATCA419850.038.6784171
TCAACGC805500.037.0296974
CAACGCA820850.036.3256155
ATCAACG829400.036.039833
AACGCAG861850.034.6197976
ACGCAGA972950.030.662037
TATCAAC1012000.029.9561882
CGCAGAG1025350.029.0750668
GTACATG1092600.025.4479681
GCAGAGT1209600.024.528479
TACATGG1114950.024.1034742
ACATGGG1156950.022.6516633
AGAGTAC1127850.020.4242410-11
CATGGGG764750.018.9983564
CAGAGTA1214100.018.50972210-11
GAGTACT711450.017.79855312-13
GAGTACA772700.016.8067021
GTACTTT794650.015.92885314-15
AGTACTT819400.015.88266912-13
Produced by FastQC (version 0.11.5)