FastQCFastQC Report
Wed 25 May 2016
SRR1003756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29842636
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG136850.015.4200291
GTATTAG179600.013.9147141
CCGGTCG23700.013.8098929
TATACTG192800.013.7525095
GTATTAT193950.013.6920431
GTATATA219000.013.6632881
GTATTAA179900.013.0216441
CCTATAC107700.012.9009353
GTATAGA180300.012.6771411
GTATAAA208950.012.6183461
TATACAG203000.012.5007165
ATAATAC201200.012.4680053
GTATAAG169050.012.4548291
CCGTGCG15900.012.2314029
TCTATAC194750.012.0530513
GTCGCCG41850.011.91738594-95
GTATAAT209100.011.8835791
TAGACCG14550.011.7359085
CTAATAC240350.011.6406473
ATTATAC221150.011.622023
Produced by FastQC (version 0.11.5)