FastQCFastQC Report
Wed 25 May 2016
SRR1003756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29842636
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG175700.015.7186081
TATACTG191350.015.1407855
GTATAGG139100.015.0194571
ATAATAC207350.013.93323
GTATATA216800.013.7948041
GTATTAA177250.013.3744961
TACCGCG19500.013.152178
GTATTAT194750.012.6380331
TATACAG200150.012.458075
GTATAAA208200.012.4172621
TAATACT258850.012.2751184
TCTATAC194250.011.9768073
TTATACT249750.011.96169954
GTATAAT213150.011.9274931
GTATAAG166350.011.8135851
CCTATAC109150.011.7929453
CTAATAC252650.011.642553
CTAGTAC60650.011.5549693
TATAGGG147200.011.5298712
GTATTGG178400.011.4166931
Produced by FastQC (version 0.11.5)