FastQCFastQC Report
Wed 25 May 2016
SRR1003755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32731608
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG136950.017.035741
GTATTAG190750.015.2140481
GTATATA239250.014.5677791
GTATTAT206500.014.2373631
GTATTAA187850.013.5809141
TATACTG205750.013.5089645
TATACAG208600.013.0288065
ATAATAC215100.012.9183933
GTATAAG173200.012.8952851
GTCGCCG55100.012.88798994-95
GTATAAA224950.012.7955991
CCTATAC116700.012.6775413
TAATACT269300.012.6255814
GTATAAT223300.012.3592511
TTATACT254350.012.1744674
GTATAGA192000.011.9783891
CTAATAC256550.011.9402053
ATTATAC237400.011.8647073
TCTATAC203200.011.7613753
TATAATA281150.011.6038552
Produced by FastQC (version 0.11.5)