FastQCFastQC Report
Wed 25 May 2016
SRR1003755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32731608
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG143000.015.6768081
TATACTG204400.014.0117765
GTATTAT205000.013.9369831
ATAATAC217250.013.9119773
GTATATA237450.013.8000411
GTATTAA189950.013.7354811
GTATTAG187900.013.7076441
CCGGTCG25700.013.30577859
CCTATAC118500.013.2353953
TATACAG207850.013.0479685
GTATAAT224950.012.5101311
TAATACT264250.012.4916744
GTATAGA198000.012.3579951
GTATAAA230200.012.3491421
TCTATAC206500.011.93520453
TTATACT256750.011.5616664
CTAATAC257200.011.4730543
GTATAAG174850.011.4026681
GTATTGG197400.011.3323911
GTATATG209700.011.30456351
Produced by FastQC (version 0.11.5)