##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003755_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32731608 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76048634701968 34.0 31.0 34.0 31.0 34.0 2 32.873607187279035 34.0 31.0 34.0 31.0 34.0 3 32.881604227937714 34.0 32.0 34.0 31.0 34.0 4 36.01119801385865 37.0 35.0 37.0 33.0 37.0 5 35.96868946982379 37.0 35.0 37.0 33.0 37.0 6 36.29698415672093 37.0 37.0 37.0 35.0 37.0 7 36.26350462219882 37.0 37.0 37.0 35.0 37.0 8 36.249116480925714 37.0 37.0 37.0 35.0 37.0 9 37.90933598495986 39.0 37.0 39.0 35.0 39.0 10-11 37.84397666927943 39.0 37.0 39.0 35.0 39.0 12-13 37.85345150167997 39.0 37.5 39.0 35.0 39.0 14-15 39.25153732135617 40.0 38.0 41.0 36.0 41.0 16-17 39.18245674028603 40.0 38.0 41.0 36.0 41.0 18-19 39.111257014931866 40.0 38.0 41.0 36.0 41.0 20-21 39.04334373062271 40.0 38.0 41.0 36.0 41.0 22-23 38.95187676083619 40.0 38.0 41.0 36.0 41.0 24-25 39.15146910900314 40.0 39.0 41.0 36.0 41.0 26-27 39.09241651067067 40.0 39.0 41.0 36.0 41.0 28-29 38.99743034317165 40.0 39.0 41.0 36.0 41.0 30-31 39.00096006587883 40.0 39.0 41.0 36.0 41.0 32-33 39.039784067437196 40.0 39.0 41.0 36.0 41.0 34-35 39.0093127413722 40.0 39.0 41.0 36.0 41.0 36-37 38.92842695048773 40.0 39.0 41.0 35.5 41.0 38-39 38.86378727559 40.0 39.0 41.0 35.0 41.0 40-41 38.78705053231727 40.0 38.0 41.0 35.0 41.0 42-43 38.687952590046905 40.0 38.0 41.0 35.0 41.0 44-45 38.587610941692816 40.0 38.0 41.0 35.0 41.0 46-47 38.47097171333593 40.0 38.0 41.0 35.0 41.0 48-49 38.175747842880185 40.0 38.0 41.0 33.5 41.0 50-51 38.07034423728892 40.0 38.0 41.0 34.0 41.0 52-53 37.99483309833113 40.0 38.0 41.0 34.0 41.0 54-55 37.83054604283419 40.0 37.5 41.0 33.0 41.0 56-57 37.63911915051653 39.5 37.0 41.0 33.0 41.0 58-59 37.42681525148413 39.0 37.0 41.0 33.0 41.0 60-61 37.18875328398165 39.0 36.5 40.5 32.0 41.0 62-63 36.94640571890021 39.0 36.0 40.0 32.0 41.0 64-65 36.650253632513255 39.0 35.5 40.0 31.5 41.0 66-67 36.1340528702409 38.0 35.0 40.0 30.5 41.0 68-69 35.78649557944114 37.5 35.0 39.5 30.0 41.0 70-71 35.48774444567465 37.0 35.0 39.0 30.0 40.5 72-73 35.1437543948345 37.0 34.0 39.0 30.0 40.0 74-75 34.72341644504603 36.0 34.0 39.0 29.0 40.0 76-77 33.660506902074594 35.0 32.5 37.5 28.5 39.0 78-79 34.10390696356867 35.0 34.0 37.5 29.0 39.0 80-81 33.92201603722005 35.0 34.0 37.0 29.0 39.0 82-83 33.57110973588588 35.0 34.0 37.0 29.0 39.0 84-85 33.23348031053042 35.0 33.5 36.0 29.0 38.5 86-87 32.91046225104492 35.0 33.0 36.0 28.0 37.5 88-89 32.60924564109408 35.0 33.0 35.5 27.0 37.0 90-91 32.25043661160796 35.0 32.5 35.0 26.0 37.0 92-93 31.913425182166428 34.0 32.0 35.0 25.5 37.0 94-95 31.57378377194301 34.0 32.0 35.0 25.0 36.0 96-97 31.206128293483168 34.0 32.0 35.0 23.5 36.0 98-99 30.798344508464112 34.0 31.0 35.0 21.0 36.0 100-101 29.550785329581117 33.5 29.5 35.0 10.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0949506776086011 1101 2 -0.1278405154046922 1101 3 -0.08531621914239906 1101 4 -0.8607080500540292 1101 5 -0.8544070566318993 1101 6 -0.11951111564840033 1101 7 -0.1184622646856397 1101 8 -0.14705338860930794 1101 9 -0.5746559285197534 1101 10-11 -0.6444429023198666 1101 12-13 -0.4608520895962158 1101 14-15 -0.5964442413323141 1101 16-17 -0.6249841581151045 1101 18-19 -0.6694636886481717 1101 20-21 -0.556812439938362 1101 22-23 -0.4843051067735402 1101 24-25 -0.4741175780853695 1101 26-27 -0.3899452237871728 1101 28-29 -0.3976085010686319 1101 30-31 -0.47053094879584734 1101 32-33 -0.4193450937138081 1101 34-35 -0.44920522686808084 1101 36-37 -0.38051305467556773 1101 38-39 -0.3807901872607289 1101 40-41 -0.42718935188093354 1101 42-43 -0.40884710858104967 1101 44-45 -0.3926088696701342 1101 46-47 -0.37741610228315636 1101 48-49 -0.2434868294787762 1101 50-51 -0.20024021055942143 1101 52-53 -0.2957638141256922 1101 54-55 -0.3032988927603597 1101 56-57 -0.15756186980890163 1101 58-59 -0.14220717129121851 1101 60-61 -0.13616065186474913 1101 62-63 -0.03111043852867823 1101 64-65 0.002998886762512143 1101 66-67 0.2885822636973572 1101 68-69 0.287100971817722 1101 70-71 0.3550517903939294 1101 72-73 0.4110750327975552 1101 74-75 0.4912755339610797 1101 76-77 0.7605909729847582 1101 78-79 0.6928725662007551 1101 80-81 0.7841469969358883 1101 82-83 0.8703508745120487 1101 84-85 0.8831025910752288 1101 86-87 0.9441401868884967 1101 88-89 1.121045161255502 1101 90-91 1.1473044748989452 1101 92-93 1.220358452978541 1101 94-95 1.3211545929519133 1101 96-97 1.4877833862305927 1101 98-99 1.5025808398518983 1101 100-101 1.8342926631625112 1102 1 0.020958448966965193 1102 2 0.02906684198192977 1102 3 0.022615874820715476 1102 4 -0.976151465060596 1102 5 -1.01221706937946 1102 6 -0.13909599884952684 1102 7 -0.13428283628953608 1102 8 -0.13983618639291961 1102 9 -0.6442757339818428 1102 10-11 -0.7089503199467444 1102 12-13 -0.4275982682826296 1102 14-15 -0.6053117465662865 1102 16-17 -0.6171330870452252 1102 18-19 -0.579983124221684 1102 20-21 -0.5046293749109552 1102 22-23 -0.3950322070512087 1102 24-25 -0.3586738649070256 1102 26-27 -0.3312483751585731 1102 28-29 -0.27413876708725127 1102 30-31 -0.32477134042517264 1102 32-33 -0.32194668411433014 1102 34-35 -0.3106601787890142 1102 36-37 -0.2542137646628362 1102 38-39 -0.270599033358323 1102 40-41 -0.2671298718646753 1102 42-43 -0.24798493375784858 1102 44-45 -0.236904300398443 1102 46-47 -0.20205136041244032 1102 48-49 -0.011901504437560106 1102 50-51 0.04140939595072268 1102 52-53 -0.008405503376764045 1102 54-55 0.03770812558925485 1102 56-57 0.08086508382283597 1102 58-59 0.16264652284425551 1102 60-61 0.19803968403633831 1102 62-63 0.33971819695019434 1102 64-65 0.42012886165631613 1102 66-67 0.7112484458837827 1102 68-69 0.8111886282620375 1102 70-71 0.8465683918207958 1102 72-73 0.9028916195983072 1102 74-75 1.0406022047676728 1102 76-77 1.3531516689550784 1102 78-79 1.2173193101690103 1102 80-81 1.3400545898031595 1102 82-83 1.4322129216604154 1102 84-85 1.5405329949843107 1102 86-87 1.6491342858165154 1102 88-89 1.8092449011678298 1102 90-91 1.8487112193344544 1102 92-93 1.932805256608443 1102 94-95 2.0268759649198387 1102 96-97 2.213498149702044 1102 98-99 2.3118210727746806 1102 100-101 2.5806771229078613 1103 1 -0.10390435827282118 1103 2 -0.05742309554533165 1103 3 -0.05148738979104195 1103 4 -0.7977398443890067 1103 5 -0.8193403678762508 1103 6 -0.13285058865150035 1103 7 -0.17660383985823813 1103 8 -0.16155097991244816 1103 9 -0.6176136634168188 1103 10-11 -0.5938717338203503 1103 12-13 -0.4248067401163098 1103 14-15 -0.5895907097968305 1103 16-17 -0.5691262450035808 1103 18-19 -0.6047978219950636 1103 20-21 -0.5376038780359238 1103 22-23 -0.49451744100623074 1103 24-25 -0.43149528538119597 1103 26-27 -0.38187512917580335 1103 28-29 -0.34018763398646 1103 30-31 -0.35429138841264773 1103 32-33 -0.3959279823752624 1103 34-35 -0.41229935869981915 1103 36-37 -0.36817816706395945 1103 38-39 -0.37046915437188943 1103 40-41 -0.3502987483346587 1103 42-43 -0.37067724037551386 1103 44-45 -0.3901272852556019 1103 46-47 -0.3367072847159207 1103 48-49 -0.2836776061171946 1103 50-51 -0.22300771862351354 1103 52-53 -0.22826268492923418 1103 54-55 -0.23336173445809294 1103 56-57 -0.1421515294701905 1103 58-59 -0.12871810134045347 1103 60-61 -0.1333701925543238 1103 62-63 0.020241401620531008 1103 64-65 0.06808014506994908 1103 66-67 0.35402524412562997 1103 68-69 0.36963065665909767 1103 70-71 0.3494521488900304 1103 72-73 0.389658708791643 1103 74-75 0.4480244854064708 1103 76-77 0.6788625438442466 1103 78-79 0.6535899722980005 1103 80-81 0.7317335014255946 1103 82-83 0.7558036233806931 1103 84-85 0.8345880197851798 1103 86-87 0.985855404778782 1103 88-89 1.1461441838858875 1103 90-91 1.0800646373187561 1103 92-93 1.0522008827632554 1103 94-95 1.166973678350729 1103 96-97 1.3170848111748548 1103 98-99 1.4343038148333882 1103 100-101 1.6168716613504976 1104 1 -0.14054234951181144 1104 2 -0.12145158277883894 1104 3 -0.1092047607464437 1104 4 -0.8099481298112252 1104 5 -0.8338948895961522 1104 6 -0.13129939256440792 1104 7 -0.17609125601924802 1104 8 -0.16124711428125948 1104 9 -0.5967389989236267 1104 10-11 -0.6251147280956602 1104 12-13 -0.4526898778396884 1104 14-15 -0.5799471346500411 1104 16-17 -0.5229279873662094 1104 18-19 -0.5988620133284357 1104 20-21 -0.549939237865587 1104 22-23 -0.4756210701863708 1104 24-25 -0.4221246717008569 1104 26-27 -0.33697087615247767 1104 28-29 -0.3845208568438352 1104 30-31 -0.39791594367917327 1104 32-33 -0.39488094734764445 1104 34-35 -0.3767833985365385 1104 36-37 -0.3108368295548303 1104 38-39 -0.3821639615615311 1104 40-41 -0.4035357836646085 1104 42-43 -0.33273455651921324 1104 44-45 -0.4302237244585072 1104 46-47 -0.3316090721942757 1104 48-49 -0.2465938158662766 1104 50-51 -0.2121693620834506 1104 52-53 -0.31157808051138147 1104 54-55 -0.2855066286576786 1104 56-57 -0.17530542371613222 1104 58-59 -0.1454243086885043 1104 60-61 -0.09926816715718445 1104 62-63 -0.02082944727821001 1104 64-65 -0.0013403738685298094 1104 66-67 0.2932672463258257 1104 68-69 0.34536962888433465 1104 70-71 0.375574270379758 1104 72-73 0.40392449446504486 1104 74-75 0.46578311191679944 1104 76-77 0.6869748661607247 1104 78-79 0.6620109653178261 1104 80-81 0.6469762470778377 1104 82-83 0.7477556383488562 1104 84-85 0.8407046335533934 1104 86-87 0.9518579821504645 1104 88-89 1.006354701246643 1104 90-91 1.0348683108995687 1104 92-93 1.1618075227806095 1104 94-95 1.1726837426518273 1104 96-97 1.216288083197714 1104 98-99 1.3789587953214628 1104 100-101 1.5514759404554788 1105 1 -0.08708742842009798 1105 2 -0.10038304855996216 1105 3 -0.10835533247077223 1105 4 -0.7647053390637026 1105 5 -0.7950932171618064 1105 6 -0.17858655744414875 1105 7 -0.16372950678244536 1105 8 -0.167468229771238 1105 9 -0.5942378058990414 1105 10-11 -0.5843879605022764 1105 12-13 -0.48092661271406456 1105 14-15 -0.5772120869738586 1105 16-17 -0.5118076762046044 1105 18-19 -0.5737778404524576 1105 20-21 -0.5646015108389051 1105 22-23 -0.42485463777456545 1105 24-25 -0.38376819602382994 1105 26-27 -0.37361257956865046 1105 28-29 -0.38009578876651773 1105 30-31 -0.3699317388358452 1105 32-33 -0.40971235303015874 1105 34-35 -0.3964304415668565 1105 36-37 -0.326276144997955 1105 38-39 -0.37433364356530774 1105 40-41 -0.3906862052547382 1105 42-43 -0.3171625228243755 1105 44-45 -0.29102457562776607 1105 46-47 -0.2762665801544699 1105 48-49 -0.046640333494373465 1105 50-51 -0.11323747075912394 1105 52-53 -0.24393764638040238 1105 54-55 -0.21566028929841963 1105 56-57 -0.14957553674215518 1105 58-59 -0.09109566103987277 1105 60-61 0.016539130479941377 1105 62-63 0.07686374894645809 1105 64-65 0.11358170976368598 1105 66-67 0.31789339831231445 1105 68-69 0.4210856158708083 1105 70-71 0.4148291008216489 1105 72-73 0.4677165341997238 1105 74-75 0.49440183315709163 1105 76-77 0.7294110821976858 1105 78-79 0.6665283282686616 1105 80-81 0.7490445687956182 1105 82-83 0.8408179765532751 1105 84-85 0.9009376121618757 1105 86-87 0.997991166695563 1105 88-89 1.075756209144778 1105 90-91 1.1179153994276305 1105 92-93 1.2324526545891068 1105 94-95 1.2545640236005653 1105 96-97 1.2954393683333656 1105 98-99 1.3834189125935303 1105 100-101 1.564083026981617 1106 1 -0.09211614182931527 1106 2 -0.08833942362475966 1106 3 -0.04462542138967507 1106 4 -0.7059408761375394 1106 5 -0.7565283857779974 1106 6 -0.17412350579572689 1106 7 -0.1788490586335314 1106 8 -0.14216488983206688 1106 9 -0.5379164202791245 1106 10-11 -0.5651978518598355 1106 12-13 -0.44937562403867304 1106 14-15 -0.5541437990482265 1106 16-17 -0.5194782692598849 1106 18-19 -0.4998425911496369 1106 20-21 -0.4966003193674169 1106 22-23 -0.4185864807462494 1106 24-25 -0.39795400255061253 1106 26-27 -0.3426526296884518 1106 28-29 -0.3478028765693608 1106 30-31 -0.3454605284633345 1106 32-33 -0.35567062154446916 1106 34-35 -0.3736571677357219 1106 36-37 -0.2833875950005904 1106 38-39 -0.34311538768142213 1106 40-41 -0.3860713619181766 1106 42-43 -0.31329020358055715 1106 44-45 -0.30625209139950726 1106 46-47 -0.24551401041461673 1106 48-49 -0.06011772182894504 1106 50-51 -0.06887781178607355 1106 52-53 -0.15908404224924055 1106 54-55 -0.17401654404547884 1106 56-57 -0.19657717247486772 1106 58-59 -0.08328805630563352 1106 60-61 0.010022348992869468 1106 62-63 0.05131381144008884 1106 64-65 0.017234790273739975 1106 66-67 0.2699892108835016 1106 68-69 0.4291192104949033 1106 70-71 0.40770058483507654 1106 72-73 0.4142972773535405 1106 74-75 0.4406954846420703 1106 76-77 0.6243877231555999 1106 78-79 0.6167544532088911 1106 80-81 0.655995795718276 1106 82-83 0.6831686053195085 1106 84-85 0.8044372906584769 1106 86-87 0.9383735025381625 1106 88-89 1.0317383943206764 1106 90-91 1.015652681167822 1106 92-93 1.1293275149847553 1106 94-95 1.2026514922866625 1106 96-97 1.1541798991165528 1106 98-99 1.2783393835534476 1106 100-101 1.2582129314076447 1107 1 -0.10150564376751703 1107 2 -0.07937674164570296 1107 3 -0.07333824461365168 1107 4 -0.6575233274072403 1107 5 -0.6984916898426974 1107 6 -0.22933070049891313 1107 7 -0.17339950109905544 1107 8 -0.13751939693742798 1107 9 -0.5042285477418602 1107 10-11 -0.5764102307551155 1107 12-13 -0.4015604086321787 1107 14-15 -0.5836864527898342 1107 16-17 -0.48183809351906604 1107 18-19 -0.511518904028101 1107 20-21 -0.5133658298446946 1107 22-23 -0.3685003348581759 1107 24-25 -0.3625931562509308 1107 26-27 -0.37758226090149805 1107 28-29 -0.39674664066475884 1107 30-31 -0.3723745289799538 1107 32-33 -0.42496468203597004 1107 34-35 -0.4038363364248312 1107 36-37 -0.3686326040771917 1107 38-39 -0.3741694512712499 1107 40-41 -0.3784934385709846 1107 42-43 -0.30740434817076334 1107 44-45 -0.299146285666815 1107 46-47 -0.30564704440030965 1107 48-49 -0.11838083252840192 1107 50-51 -0.10774184327916458 1107 52-53 -0.21159113758145054 1107 54-55 -0.11536975255014426 1107 56-57 -0.11736009840954154 1107 58-59 -0.08291580122048714 1107 60-61 -0.04014511572584212 1107 62-63 0.048550990190413756 1107 64-65 -0.02778959905452183 1107 66-67 0.32360205499986705 1107 68-69 0.34442423833969116 1107 70-71 0.30952601424232995 1107 72-73 0.33106634804036617 1107 74-75 0.3716158146840698 1107 76-77 0.5708897103552175 1107 78-79 0.5375035390351073 1107 80-81 0.558338924605529 1107 82-83 0.6511560520018591 1107 84-85 0.8076237812444944 1107 86-87 0.8269535649191724 1107 88-89 0.932304097877477 1107 90-91 0.9161403010273546 1107 92-93 0.9839499419400504 1107 94-95 1.0091597483400392 1107 96-97 0.8370557598964545 1107 98-99 1.018748107465104 1107 100-101 1.052276208286802 1108 1 -0.0986723557234015 1108 2 -0.14236287885451304 1108 3 -0.08003973204521486 1108 4 -0.6384437924655373 1108 5 -0.6227412079655039 1108 6 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60-61 -0.03401189331332688 2316 62-63 -0.01547858694770099 2316 64-65 -0.04919832756380771 2316 66-67 0.25383069260271895 2316 68-69 -0.280486050021338 2316 70-71 -0.41337009488828613 2316 72-73 -0.14965629696155247 2316 74-75 -0.03321336173220146 2316 76-77 -0.206905078533822 2316 78-79 -0.2659047719811909 2316 80-81 -0.20591160904520933 2316 82-83 -0.22519491655723556 2316 84-85 -0.2453589591279055 2316 86-87 -0.20846649999116806 2316 88-89 -0.3537205158831327 2316 90-91 -0.25687076593239766 2316 92-93 -0.21738621306522177 2316 94-95 -0.24507857752550422 2316 96-97 -0.22262028717280558 2316 98-99 -0.19242167662686782 2316 100-101 -0.5351906058488503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57.0 3 84.0 4 137.0 5 423.0 6 758.0 7 2126.0 8 5061.0 9 10524.0 10 15566.0 11 20786.0 12 25416.0 13 31702.0 14 38194.0 15 43593.0 16 49591.0 17 56131.0 18 60987.0 19 67197.0 20 72991.0 21 80167.0 22 87637.0 23 98906.0 24 117538.0 25 142073.0 26 176174.0 27 221605.0 28 278656.0 29 352568.0 30 444447.0 31 563750.0 32 724567.0 33 962472.0 34 1357829.0 35 2063680.0 36 3745501.0 37 8325222.0 38 1.0983302E7 39 1504142.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.79547354534642 21.15909491692945 14.776791348835483 24.268640188888646 2 14.92288943060171 21.494331008259 39.78010526228959 23.802674298849706 3 16.478319843107712 26.864032593132414 31.227542565605372 25.4301049981545 4 12.311721132910916 18.822364494560045 40.2552103199875 28.610704052541543 5 12.47748005234218 37.82444130258838 37.28451554230405 12.413563102765385 6 28.701911517283456 39.68442412971689 19.18784880294541 12.425815550054244 7 25.63911522382847 32.32689169499505 25.392703133391066 16.64128994778542 8 23.847318211251785 36.57457066343065 22.18583231711369 17.392278808203876 9 24.38842444822292 17.379086134667514 21.951626815989414 36.28086260112015 10-11 22.751385236815516 26.97286890233591 31.00381691505043 19.271928945798145 12-13 23.64870367271916 24.35396428726315 30.23907748698187 21.758254553035815 14-15 21.17651416906426 26.35264552772118 26.69195516902126 25.778885134193303 16-17 21.435350157418746 29.532333353783148 27.512280886570267 21.520035602227843 18-19 21.536250220276376 29.1190781094531 28.520670295208227 20.824001375062295 20-21 21.668258705774555 28.705962139104198 28.2705023230145 21.35527683210675 22-23 21.646128720593257 28.234808995635046 28.375877530978617 21.74318475279308 24-25 21.63166646540854 28.446547712802587 28.25039627228705 21.671389549501825 26-27 21.57877757416698 28.526899470387367 28.137494008268384 21.756828947177272 28-29 21.82379438994818 28.34509998708738 28.31386254330168 21.517243079662755 30-31 21.800447834565755 28.59658934427598 28.105669380051296 21.497293441106965 32-33 21.611473169243762 28.516060361869116 28.174050770587456 21.698415698299662 34-35 21.85910311208544 28.27169581087366 28.29281165402677 21.57638942301413 36-37 21.969955771410866 28.326015367979295 28.033205606731247 21.670823253878584 38-39 21.719000402174913 28.57351551025352 28.18551074403703 21.521973343534533 40-41 21.755841486942334 28.263253556482386 28.303687692835116 21.677217263740157 42-43 21.72799282985101 28.399076069745377 28.25555577094599 21.61737532945762 44-45 21.59826604098836 28.40273530705066 28.297472317996974 21.701526333964008 46-47 21.691667367203532 28.242606413255483 28.309324816027154 21.75640140351383 48-49 21.68016233357446 28.365757957172168 28.171858536540807 21.782221172712568 50-51 21.748653368102392 28.275364509967382 28.114068552469945 21.861913569460274 52-53 21.715538915305217 28.199440276624983 28.272588479399424 21.81243232867038 54-55 21.619657935756894 28.52203666447744 28.21141621687218 21.646889182893478 56-57 21.637120006980474 28.333584647249893 28.166733694743673 21.862561651025956 58-59 21.527387533658164 28.09766062908784 28.347423315429715 22.02752852182428 60-61 21.630648378077154 28.272903305437698 28.320951792034098 21.77549652445105 62-63 21.51457853918915 28.3338483053104 28.228249898733043 21.92332325676741 64-65 21.57852826119056 28.21157127952635 28.17564534469512 22.034255114587967 66-67 21.49231846809794 28.16003162947781 28.201830823966105 22.14581907845814 68-69 21.461827685751942 28.403911029943885 28.188244552382493 21.946016731921684 70-71 21.59057050357946 28.129674670684356 28.244299075719674 22.03545575001651 72-73 21.481788038372244 28.236204770608982 28.097783166127293 22.184224024891478 74-75 21.418972222187 28.296762624738207 28.12993250951022 22.15433264356457 76-77 21.51020754708738 28.066389392171505 28.135060825560963 22.288342235180153 78-79 21.51211517749646 28.263964137983923 28.098296850586213 22.125623833933403 80-81 21.44348734291126 28.251538063592964 28.079849126740875 22.225125466754903 82-83 21.453989211154585 28.17601800145083 28.155378914750962 22.21461387264362 84-85 21.40458375822286 28.16617668321365 28.128091102322617 22.30114845624088 86-87 21.48784492949068 28.103812239028166 28.13440825893646 22.2739345725447 88-89 21.382981747719096 28.12298686730396 28.211283437336387 22.28274794764055 90-91 21.375638600651182 28.17602014063807 28.14303615817139 22.305305100539353 92-93 21.32665973159828 28.148377528087003 28.01901600090056 22.505946739414156 94-95 21.324841560244533 27.876788887074426 28.249762189515444 22.548607363165594 96-97 21.433961843344214 27.9933598888895 28.060439248207686 22.5122390195586 98-99 21.251733934074554 28.038704046670304 28.01694828855492 22.692613730700224 100-101 21.257196093660124 27.898906622554705 28.12634613006001 22.717551153725164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3633.0 1 1953.0 2 213.0 3 149.0 4 129.0 5 114.0 6 124.0 7 121.0 8 115.5 9 128.5 10 155.0 11 181.0 12 205.0 13 265.5 14 363.5 15 517.0 16 807.0 17 1458.0 18 2751.0 19 4862.5 20 8512.0 21 14587.5 22 24095.5 23 38836.5 24 60534.0 25 91001.5 26 131933.5 27 185333.0 28 250858.0 29 327270.0 30 409828.5 31 497410.5 32 590977.0 33 681680.5 34 776594.0 35 889308.0 36 1006750.0 37 1126023.0 38 1232239.5 39 1312193.5 40 1386298.5 41 1451207.5 42 1496776.5 43 1556377.5 44 1577555.5 45 1548123.0 46 1527749.5 47 1469921.5 48 1382101.5 49 1290783.0 50 1199357.5 51 1105082.0 52 970326.0 53 831362.0 54 721810.0 55 627828.0 56 539876.0 57 455822.5 58 387945.0 59 332892.5 60 283140.5 61 233650.0 62 187318.0 63 145907.5 64 110006.5 65 80410.0 66 55944.5 67 36955.5 68 23823.5 69 15123.0 70 9323.0 71 5732.0 72 3643.5 73 2404.0 74 1631.0 75 1158.5 76 874.5 77 661.5 78 532.5 79 403.0 80 309.0 81 262.0 82 197.0 83 139.5 84 109.0 85 82.5 86 65.5 87 57.5 88 43.5 89 31.5 90 19.0 91 15.5 92 15.5 93 10.5 94 4.5 95 4.5 96 5.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052875495759328414 2 1.1609573229643957E-4 3 0.3578498190495255 4 5.285410970337907E-4 5 2.1691571034334763E-4 6 5.499271529831349E-5 7 0.004512457805311612 8 9.165452549718914E-6 9 6.11030169981261E-6 10-11 6.140853208311672E-4 12-13 7.103225726032158E-4 14-15 1.5275754249531524E-6 16-17 0.0032751217110995583 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.20790839240162E-5 26-27 1.3289906197092426E-4 28-29 1.726160230197062E-4 30-31 3.100978112654899E-4 32-33 6.599125835797618E-4 34-35 7.958667964005924E-4 36-37 0.0019232174600160188 38-39 0.0025189718757477482 40-41 0.001836145660793689 42-43 0.0010677752220422535 44-45 0.002231787695856556 46-47 0.002317331919653932 48-49 0.0029909926820582723 50-51 0.002387600389201777 52-53 0.002434955227375325 54-55 0.0025250821774475607 56-57 9.150176795469383E-4 58-59 4.2008324186211695E-4 60-61 2.6885327479175483E-4 62-63 1.634505704699873E-4 64-65 0.0011579021721144895 66-67 3.5439749858913135E-4 68-69 8.584973888236716E-4 70-71 0.0010952715796914102 72-73 2.5816024681708273E-4 74-75 3.2842871636492774E-4 76-77 0.0011762330772139274 78-79 1.2373360942120532E-4 80-81 8.859937464728284E-5 82-83 7.332362039775131E-5 84-85 1.8483662641933144E-4 86-87 6.91991667503778E-4 88-89 0.0013396836476839147 90-91 0.0017399084090216406 92-93 0.00261673670294475 94-95 0.002178322555983195 96-97 6.965743937786375E-4 98-99 1.3289906197092426E-4 100-101 1.4511966537054946E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.2731608E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.38430304786521 #Duplication Level Percentage of deduplicated Percentage of total 1 60.552232648603386 23.84807480857434 2 20.127666649046684 15.854282459049287 3 7.915243856698552 9.352090882499073 4 3.7605782177617018 5.924310086541108 5 2.094031855596023 4.123599259633865 6 1.2561660073216392 2.968393362646938 7 0.8668965847329405 2.3899482462997046 8 0.5892944012133484 1.8567159425437425 9 0.44159860561514863 1.5652847978155555 >10 2.171052168586783 14.982142798429107 >50 0.12671490291305804 3.442497701269952 >100 0.0824620842995569 6.314492314437582 >500 0.009875329233373665 2.683778433190613 >1k 0.006000198590215574 4.234260470007689 >5k 1.8648978753769857E-4 0.4601284370613844 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 6.11030169981261E-6 0.0 3.055150849906305E-6 0.0 2 0.0 1.222060339962522E-5 0.0 1.222060339962522E-5 0.0 3 0.0 1.222060339962522E-5 0.0 4.888241359850088E-5 0.0 4 0.0 1.5275754249531523E-5 0.0 7.943392209756392E-5 0.0 5 6.11030169981261E-6 2.444120679925044E-5 0.0 9.165452549718914E-5 0.0 6 6.11030169981261E-6 2.444120679925044E-5 0.0 9.470967634709544E-5 0.0 7 6.11030169981261E-6 2.444120679925044E-5 0.0 1.0998543059662697E-4 0.0 8 6.11030169981261E-6 2.444120679925044E-5 0.0 1.4664724079550262E-4 0.0 9 1.222060339962522E-5 2.444120679925044E-5 0.0 3.7578355453847545E-4 0.0 10-11 1.222060339962522E-5 3.666181019887566E-5 0.0 6.186680471060267E-4 0.0 12-13 1.222060339962522E-5 5.6520290723266635E-5 0.0 8.111425506501239E-4 0.0 14-15 1.6803329674484674E-5 7.332362039775131E-5 0.0 0.0011319333898902858 0.0 16-17 1.8330905099437827E-5 8.248907294747022E-5 0.0 0.0015688199614268873 0.0 18-19 1.8330905099437827E-5 8.859937464728284E-5 0.0 0.0020790301533612403 0.0 20-21 1.8330905099437827E-5 9.165452549718914E-5 0.0 0.002900865731986036 0.0 22-23 2.138605594934413E-5 9.318210092214228E-5 0.0 0.003728811612310645 0.0 24-25 2.7496357649156742E-5 1.1609573229643958E-4 0.0 0.0047247907893801 0.0 26-27 2.7496357649156742E-5 1.2220603399625219E-4 0.0 0.006044615956539623 0.0 28-29 2.7496357649156742E-5 1.2678876027111166E-4 0.0 0.009026443186048177 0.0 30-31 2.7496357649156742E-5 1.4206451452064317E-4 0.0 0.01659557941669105 0.0 32-33 2.7496357649156742E-5 1.6497814589494045E-4 0.0 0.03202103605786798 0.0 34-35 2.7496357649156742E-5 2.046951069437224E-4 0.0 0.05130667579790153 0.0 36-37 2.9023933074109896E-5 2.199708611932539E-4 0.0 0.08310315826830139 0.0 38-39 3.0551508499063046E-5 2.3677419086773859E-4 0.0 0.1394844396278973 0.0 40-41 3.2079083924016203E-5 2.4899479426736384E-4 0.0 0.21017451999302936 0.0 42-43 3.3606659348969354E-5 2.6427054851689536E-4 0.0 0.281694073813911 0.0 44-45 3.3606659348969354E-5 2.8107387819138003E-4 0.0 0.36237296988281176 0.0 46-47 3.3606659348969354E-5 2.902393307410989E-4 0.0 0.45888976795762676 0.0 48-49 3.3606659348969354E-5 2.902393307410989E-4 0.0 0.5681984826409995 0.0 50-51 3.3606659348969354E-5 3.0857023584053675E-4 0.0 0.6902349557650819 3.055150849906305E-6 52-53 3.6661810198875654E-5 3.0857023584053675E-4 0.0 0.8118925901837759 6.11030169981261E-6 54-55 3.6661810198875654E-5 3.1162538669044307E-4 0.0 0.9369551902246904 6.11030169981261E-6 56-57 3.6661810198875654E-5 3.1926326381520885E-4 0.0 1.0776693280696752 6.11030169981261E-6 58-59 3.6661810198875654E-5 3.20790839240162E-4 0.0 1.2385459339486162 6.11030169981261E-6 60-61 3.971696104878196E-5 3.223184146651151E-4 0.0 1.4152696072860214 6.11030169981261E-6 62-63 4.888241359850088E-5 3.2384599009006827E-4 0.0 1.597301605225139 6.11030169981261E-6 64-65 5.4992715298313485E-5 3.2384599009006827E-4 0.0 1.7782780485456138 6.11030169981261E-6 66-67 6.72133186979387E-5 3.2384599009006827E-4 0.0 1.972704793482801 6.11030169981261E-6 68-69 7.637877124765761E-5 3.3453901806474037E-4 0.0 2.196529727473212 6.11030169981261E-6 70-71 7.637877124765761E-5 3.375941689146467E-4 0.0 2.4429627777529292 6.11030169981261E-6 72-73 7.943392209756392E-5 3.482871968893187E-4 0.0 2.6992792410320936 6.11030169981261E-6 74-75 8.554422379737652E-5 3.681456774137097E-4 0.0 2.9518057896819494 6.11030169981261E-6 76-77 9.165452549718914E-5 3.91059308788007E-4 0.0 3.2125537492689022 6.11030169981261E-6 78-79 9.776482719700175E-5 3.91059308788007E-4 0.0 3.507427743849309 9.165452549718914E-6 80-81 1.1609573229643958E-4 3.9564203506286643E-4 0.0 3.8315425872141695 1.222060339962522E-5 82-83 1.2067845857129903E-4 4.048074876125854E-4 0.0 4.170902633320062 1.222060339962522E-5 84-85 1.2373360942120534E-4 4.124453647373511E-4 0.0 4.50746416124744 1.222060339962522E-5 86-87 1.252611848461585E-4 4.155005155872574E-4 0.0 4.845634226097294 1.222060339962522E-5 88-89 1.2678876027111166E-4 4.1855566643716373E-4 0.0 5.21571534157442 1.222060339962522E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 14300 0.0 15.676808 1 TATACTG 20440 0.0 14.011776 5 GTATTAT 20500 0.0 13.936983 1 ATAATAC 21725 0.0 13.911977 3 GTATATA 23745 0.0 13.800041 1 GTATTAA 18995 0.0 13.735481 1 GTATTAG 18790 0.0 13.707644 1 CCGGTCG 2570 0.0 13.3057785 9 CCTATAC 11850 0.0 13.235395 3 TATACAG 20785 0.0 13.047968 5 GTATAAT 22495 0.0 12.510131 1 TAATACT 26425 0.0 12.491674 4 GTATAGA 19800 0.0 12.357995 1 GTATAAA 23020 0.0 12.349142 1 TCTATAC 20650 0.0 11.9352045 3 TTATACT 25675 0.0 11.561666 4 CTAATAC 25720 0.0 11.473054 3 GTATAAG 17485 0.0 11.402668 1 GTATTGG 19740 0.0 11.332391 1 GTATATG 20970 0.0 11.3045635 1 >>END_MODULE