FastQCFastQC Report
Fri 27 May 2016
SRR940235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5009557
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58150.036.771461
ATCAACG64450.033.396893
TCAACGC65000.033.1867374
GGTATCA37100.032.9160231
CAACGCA66950.032.2182045
AACGCAG71600.030.3124776
TATCAAC69050.030.0722472
ACGCAGA82600.026.1612387
GTACATG120600.026.0436741
TACATGG126600.024.2088472
ACATGGG132550.023.6230743
CGCAGAG92300.023.204928
CATGGGG110950.021.1125984
GTACCTG45950.020.4751831
GCAGAGT117950.018.1595699
CCTATAA26700.017.7965931
AGAGTAC105750.016.88823510-11
TACCTGG55100.016.385142
TATAAGC27200.016.2459373
TATAGGA27250.016.0414434
Produced by FastQC (version 0.11.5)