##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940235_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5009557 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8306179568373 33.0 31.0 34.0 30.0 34.0 2 30.806838608683364 33.0 31.0 34.0 26.0 34.0 3 31.6586981643287 34.0 31.0 34.0 28.0 34.0 4 35.39564216955711 37.0 35.0 37.0 33.0 37.0 5 35.55417155648693 37.0 35.0 37.0 35.0 37.0 6 35.70127717880044 37.0 36.0 37.0 35.0 37.0 7 35.673645993048886 37.0 37.0 37.0 35.0 37.0 8 35.66602855302375 37.0 37.0 37.0 35.0 37.0 9 37.41228695471476 39.0 38.0 39.0 35.0 39.0 10-11 37.34491662636037 39.0 38.0 39.0 35.0 39.0 12-13 37.268301169145296 39.0 38.0 39.0 35.0 39.0 14-15 38.69070328973201 41.0 39.0 41.0 35.0 41.0 16-17 38.607796258232014 41.0 39.0 41.0 35.0 41.0 18-19 38.53251245569219 41.0 39.0 41.0 34.5 41.0 20-21 38.45478163039167 40.5 39.0 41.0 34.0 41.0 22-23 38.35192103812772 40.0 38.5 41.0 34.0 41.0 24-25 38.25094843715722 40.0 38.0 41.0 34.0 41.0 26-27 38.06055076327108 40.0 38.0 41.0 33.5 41.0 28-29 37.89638085762873 40.0 38.0 41.0 33.0 41.0 30-31 37.8850963268808 40.0 38.0 41.0 33.5 41.0 32-33 37.92316286649698 40.0 38.0 41.0 33.0 41.0 34-35 37.93426354865311 40.0 38.0 41.0 33.5 41.0 36-37 38.04693409018003 40.0 38.0 41.0 33.5 41.0 38-39 37.97679874687522 40.0 38.0 41.0 33.0 41.0 40-41 37.85975765921019 40.0 38.0 41.0 33.0 41.0 42-43 37.601699910790515 40.0 38.0 41.0 33.0 41.0 44-45 37.56550838726857 40.0 38.0 41.0 33.0 41.0 46-47 37.42264435757493 40.0 38.0 41.0 32.0 41.0 48-49 37.258781764535264 40.0 37.5 41.0 32.0 41.0 50-51 36.33653804118807 39.0 36.0 40.0 31.0 40.5 52-53 36.41718529203281 39.0 36.5 40.0 30.5 41.0 54-55 36.78574612485694 40.0 36.5 41.0 31.0 41.0 56-57 36.61548266643138 40.0 36.0 41.0 31.0 41.0 58-59 36.352978117625966 39.0 35.5 41.0 30.5 41.0 60-61 36.08395163484516 39.0 35.0 41.0 30.0 41.0 62-63 35.741275825387355 38.5 35.0 40.5 29.5 41.0 64-65 35.3556787556265 37.5 35.0 40.0 29.0 41.0 66-67 34.975167764335254 37.0 35.0 40.0 29.0 41.0 68-69 34.58112853092599 36.5 34.5 39.0 28.5 41.0 70-71 34.15647112109913 36.0 34.0 39.0 28.0 41.0 72-73 33.70626793946052 35.5 34.0 38.5 27.5 40.0 74-75 33.25664484903555 35.0 34.0 37.0 26.0 39.0 76-77 32.806308921128156 35.0 33.5 37.0 26.0 39.0 78-79 32.37114060185361 35.0 33.0 36.0 25.5 38.5 80-81 31.980333889803028 35.0 33.0 36.0 25.0 37.0 82-83 31.631074564078222 35.0 33.0 35.0 24.0 37.0 84-85 31.330718963772647 35.0 32.0 35.0 23.0 36.5 86-87 31.026020863721083 35.0 32.0 35.0 21.0 36.0 88-89 30.71660019039608 34.5 32.0 35.0 19.5 36.0 90-91 30.43629596389461 34.0 31.0 35.0 18.0 35.0 92-93 30.13671498298153 34.0 31.0 35.0 14.0 35.0 94-95 29.80569549363347 34.0 31.0 35.0 4.5 35.0 96-97 29.425440313384996 34.0 30.5 35.0 2.0 35.0 98-99 28.924229827108466 34.0 30.0 35.0 2.0 35.0 100-101 26.900737530284616 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.577436121821723 1101 2 1.664657736951245 1101 3 0.968717923160046 1101 4 0.5315043588636073 1101 5 0.41589121691693975 1101 6 0.368309037373578 1101 7 0.403529888685469 1101 8 0.3536503341223849 1101 9 0.3977607733307238 1101 10-11 0.38889364767339174 1101 12-13 0.3641849479625634 1101 14-15 0.4453819233038985 1101 16-17 0.5133570882716825 1101 18-19 0.550367289419242 1101 20-21 0.5542732428397485 1101 22-23 0.4952198765249136 1101 24-25 0.48581269959358764 1101 26-27 0.5420802090791383 1101 28-29 0.5073013132400277 1101 30-31 0.4901873246263406 1101 32-33 0.5188604248609252 1101 34-35 0.580365419970839 1101 36-37 0.6259018491926298 1101 38-39 0.6145402441818391 1101 40-41 0.585065114861159 1101 42-43 0.7058411321133846 1101 44-45 0.6071307370788546 1101 46-47 0.6481621291102257 1101 48-49 0.6166704247542896 1101 50-51 0.4134835913431729 1101 52-53 0.49420760384258955 1101 54-55 0.6334432037308346 1101 56-57 0.7043933974370233 1101 58-59 0.6770359272628852 1101 60-61 0.5905173752019124 1101 62-63 0.7137214079164877 1101 64-65 0.7115544616988956 1101 66-67 0.6953131218813127 1101 68-69 0.6184117214769671 1101 70-71 0.593651514762314 1101 72-73 0.7247753997077595 1101 74-75 0.6923947355252764 1101 76-77 0.7470088594536222 1101 78-79 0.7336779375849787 1101 80-81 0.7483367687626341 1101 82-83 0.7972023315913468 1101 84-85 0.700984353400262 1101 86-87 0.7062254854456711 1101 88-89 0.624671486793396 1101 90-91 0.726942650405416 1101 92-93 0.7896002369640875 1101 94-95 0.7152081415693523 1101 96-97 0.6477745663057348 1101 98-99 0.6913262823587516 1101 100-101 0.8408796853348903 1102 1 0.4182746524776597 1102 2 1.0345895536640413 1102 3 0.6700680739381575 1102 4 0.3940084413121028 1102 5 0.36706560679584044 1102 6 0.25672299641887264 1102 7 0.2703529789193837 1102 8 0.3166310797504508 1102 9 0.37243774574332633 1102 10-11 0.3866878934065525 1102 12-13 0.39646737738857496 1102 14-15 0.37388453142693834 1102 16-17 0.47462453238691893 1102 18-19 0.4189238209293009 1102 20-21 0.40896229152831864 1102 22-23 0.4189069961558616 1102 24-25 0.4699518540798806 1102 26-27 0.5052317399001822 1102 28-29 0.48105763783653543 1102 30-31 0.46976517812752405 1102 32-33 0.44431581571165424 1102 34-35 0.45690800737219917 1102 36-37 0.4956630702631628 1102 38-39 0.44998605703500516 1102 40-41 0.4468778584693709 1102 42-43 0.600130831567462 1102 44-45 0.4384128789564272 1102 46-47 0.48157183558526384 1102 48-49 0.44012715497036226 1102 50-51 0.2790569263344409 1102 52-53 0.3662512524436181 1102 54-55 0.5024892499588418 1102 56-57 0.5381871336578783 1102 58-59 0.5376375365863524 1102 60-61 0.5568063968226369 1102 62-63 0.5408769359829719 1102 64-65 0.5811746096508088 1102 66-67 0.5646542037950866 1102 68-69 0.5116158166064082 1102 70-71 0.4821243293041917 1102 72-73 0.5166949665161411 1102 74-75 0.43044242572662483 1102 76-77 0.47053004638986096 1102 78-79 0.451060761598697 1102 80-81 0.3962804847110277 1102 82-83 0.5089028043118766 1102 84-85 0.42806203298866663 1102 86-87 0.39380501381695865 1102 88-89 0.42763304609822583 1102 90-91 0.2749758437681855 1102 92-93 0.3858863603544016 1102 94-95 0.3372974718923665 1102 96-97 0.4356364683751188 1102 98-99 0.45522110787068826 1102 100-101 0.5395884943062477 1103 1 0.40850215281714597 1103 2 1.4237675093052609 1103 3 0.7690057138309889 1103 4 0.41042098724270204 1103 5 0.34151692038435044 1103 6 0.24251599236587396 1103 7 0.210091315373802 1103 8 0.2540837893908474 1103 9 0.2554195557756671 1103 10-11 0.30366660027267756 1103 12-13 0.3600394184047957 1103 14-15 0.34793285088479564 1103 16-17 0.40063930767113476 1103 18-19 0.33225352429123944 1103 20-21 0.3090706131126737 1103 22-23 0.3556577006168027 1103 24-25 0.35313692340996994 1103 26-27 0.41985828715161233 1103 28-29 0.3468177530278638 1103 30-31 0.38195641113266277 1103 32-33 0.35407414474968135 1103 34-35 0.3601573547550032 1103 36-37 0.3622492063191487 1103 38-39 0.3727021378810491 1103 40-41 0.4901862322249073 1103 42-43 0.5772642856330421 1103 44-45 0.44876757473053175 1103 46-47 0.26585018935912785 1103 48-49 0.32690776963156765 1103 50-51 0.3935933245969636 1103 52-53 0.42401898193425325 1103 54-55 0.5070343998086244 1103 56-57 0.5798054242023838 1103 58-59 0.5287195263709563 1103 60-61 0.52872018668468 1103 62-63 0.4090636793201625 1103 64-65 0.5181168403523628 1103 66-67 0.5488463195990789 1103 68-69 0.5001415461547154 1103 70-71 0.44152583697474057 1103 72-73 0.4781339559379276 1103 74-75 0.5414615217773431 1103 76-77 0.5583396547085826 1103 78-79 0.5986425648822618 1103 80-81 0.572653436576303 1103 82-83 0.706511793725479 1103 84-85 0.6374971481367986 1103 86-87 0.5940800448618084 1103 88-89 0.6499168592908546 1103 90-91 0.5235752896586412 1103 92-93 0.6868758845534693 1103 94-95 0.5088628945548272 1103 96-97 0.40197068932786806 1103 98-99 0.47508415035063223 1103 100-101 0.48951696813475465 1104 1 0.5422148824281159 1104 2 1.5500649105726474 1104 3 0.8519541328926046 1104 4 0.5282364685198502 1104 5 0.38024115309426065 1104 6 0.30479963384675557 1104 7 0.3329034704164613 1104 8 0.32427121857989505 1104 9 0.3066881165919497 1104 10-11 0.3214369451521648 1104 12-13 0.3132839018883473 1104 14-15 0.3220582223569508 1104 16-17 0.34134678508683436 1104 18-19 0.32493865343391803 1104 20-21 0.34468619938609635 1104 22-23 0.3172297017061041 1104 24-25 0.34162859759054953 1104 26-27 0.344393939789164 1104 28-29 0.3593577584849825 1104 30-31 0.230941814781211 1104 32-33 0.31303297204494385 1104 34-35 0.2665912299985891 1104 36-37 0.35460618904293284 1104 38-39 0.467203983872821 1104 40-41 0.37070800862907305 1104 42-43 0.4662845007227787 1104 44-45 0.3036475060903925 1104 46-47 0.29535595352367494 1104 48-49 0.26631843021970525 1104 50-51 0.236452365386981 1104 52-53 0.3263882436284149 1104 54-55 0.4399161177458808 1104 56-57 0.41603700563655366 1104 58-59 0.4172222365397076 1104 60-61 0.3937004290900248 1104 62-63 0.39981415827411126 1104 64-65 0.3764503699692625 1104 66-67 0.43668446655522075 1104 68-69 0.3670814924709376 1104 70-71 0.3567578692876978 1104 72-73 0.37661964810125426 1104 74-75 0.2919127975366891 1104 76-77 0.239531503497723 1104 78-79 0.29922906016735595 1104 80-81 0.18004107791038848 1104 82-83 0.18902932405304895 1104 84-85 0.15067855975701505 1104 86-87 0.07900242375507105 1104 88-89 0.09601893680018847 1104 90-91 0.11179956828567228 1104 92-93 0.104717341423882 1104 94-95 -0.0074523936586921025 1104 96-97 -0.0366409427801031 1104 98-99 -0.009206636557671288 1104 100-101 0.03880399125060663 1105 1 0.20031343100006538 1105 2 1.18699404174318 1105 3 0.45308337191345416 1105 4 0.25429097430473746 1105 5 0.09106688725564283 1105 6 -0.02404065459077742 1105 7 0.004197305295143394 1105 8 0.042162993131356075 1105 9 -0.14911411323404167 1105 10-11 -0.08879305219985412 1105 12-13 -0.1777025544150277 1105 14-15 -0.14351867512594652 1105 16-17 -0.27634474404310083 1105 18-19 -0.2801008060594654 1105 20-21 -0.28879369556328527 1105 22-23 -0.29285597516496864 1105 24-25 -0.21769705002854067 1105 26-27 -0.20555902223290445 1105 28-29 -0.20444964215687378 1105 30-31 -0.30975698321183387 1105 32-33 -0.3219650333448172 1105 34-35 -0.2670995385245689 1105 36-37 -0.31215607065566786 1105 38-39 -0.2422990926884978 1105 40-41 -0.348669379903761 1105 42-43 -0.17028600573237895 1105 44-45 -0.11770610675731774 1105 46-47 -0.3202811873947624 1105 48-49 -0.39744876790629036 1105 50-51 -0.47578687854367985 1105 52-53 -0.4348158803360107 1105 54-55 -0.18696867687649643 1105 56-57 -0.25321562924874996 1105 58-59 -0.26781971233759094 1105 60-61 -0.43883725818267294 1105 62-63 -0.318224539922916 1105 64-65 -0.21811247249342358 1105 66-67 -0.3163812338719083 1105 68-69 -0.47507070749623637 1105 70-71 -0.3233893974239308 1105 72-73 -0.6006006789251828 1105 74-75 -0.6313206966756653 1105 76-77 -0.6126631366725235 1105 78-79 -0.4871143503437736 1105 80-81 -0.5468645851277074 1105 82-83 -0.5889287501705844 1105 84-85 -0.5688636815022114 1105 86-87 -0.6299274394046677 1105 88-89 -0.6926652100307074 1105 90-91 -0.7628491768855419 1105 92-93 -0.8257588118642438 1105 94-95 -0.8795107314398152 1105 96-97 -1.127055271770459 1105 98-99 -1.2382861575477975 1105 100-101 -1.304073345218935 1106 1 0.21438056043612974 1106 2 1.2412934071396613 1106 3 0.48337134849700547 1106 4 0.2484930794045468 1106 5 0.08552761927953867 1106 6 0.12309555954421114 1106 7 0.14190580765645677 1106 8 0.1666167361546016 1106 9 -0.041242720639409924 1106 10-11 0.13187934672801305 1106 12-13 0.09362366731350846 1106 14-15 0.12383717838339692 1106 16-17 0.019993050687070024 1106 18-19 -0.09305150808583562 1106 20-21 -0.13359882561406522 1106 22-23 -0.04263576796962809 1106 24-25 -0.10331198321211588 1106 26-27 0.06808584895930636 1106 28-29 -0.042143214498587156 1106 30-31 -0.026579122655867593 1106 32-33 -0.034181231140131274 1106 34-35 -0.07342429691344421 1106 36-37 -0.01987492374389177 1106 38-39 -0.08801532589022543 1106 40-41 -0.056062136127565054 1106 42-43 0.07712049778604069 1106 44-45 0.00274786471265287 1106 46-47 -0.11557140889579642 1106 48-49 -0.23995290461875385 1106 50-51 -0.5018327473681765 1106 52-53 -0.1657014061175346 1106 54-55 -0.14540399198983778 1106 56-57 -0.08648895529740486 1106 58-59 -0.17745432094225322 1106 60-61 -0.09836465879422462 1106 62-63 -0.16084402998764347 1106 64-65 -0.33777945160288425 1106 66-67 -0.1613439742682914 1106 68-69 -0.16786665052354977 1106 70-71 -0.32124373090567104 1106 72-73 -0.3178160791344524 1106 74-75 -0.41828530174721834 1106 76-77 -0.4936802971080354 1106 78-79 -0.4510687583673203 1106 80-81 -0.5041242141663922 1106 82-83 -0.393202596580565 1106 84-85 -0.4037020935846911 1106 86-87 -0.5106230464853496 1106 88-89 -0.6927868254221039 1106 90-91 -0.8358452019502707 1106 92-93 -1.0271604210105494 1106 94-95 -1.01755568892575 1106 96-97 -1.0248281657854932 1106 98-99 -1.0520791038362631 1106 100-101 -1.0802416769288463 1107 1 0.35577289615652674 1107 2 1.4171191078431953 1107 3 0.704270075311829 1107 4 0.3196167752825758 1107 5 0.25202219796022973 1107 6 0.20172313056203706 1107 7 0.21237158229705244 1107 8 0.16643325303945744 1107 9 0.08548742654090091 1107 10-11 0.19764793114903512 1107 12-13 0.22824659406245473 1107 14-15 0.1795564386554318 1107 16-17 0.20137810438621528 1107 18-19 0.18269602970951837 1107 20-21 0.11366118889267085 1107 22-23 0.08797651338785784 1107 24-25 0.05889442203400108 1107 26-27 0.15299844700773946 1107 28-29 0.08148853432752645 1107 30-31 0.036268085853031096 1107 32-33 0.029965274902600925 1107 34-35 0.1137411490699094 1107 36-37 0.09808615188064351 1107 38-39 0.1350188500028935 1107 40-41 0.04255807197015571 1107 42-43 0.20438255498905988 1107 44-45 -0.05553911984572579 1107 46-47 -0.12665977781348658 1107 48-49 -0.042612039002044355 1107 50-51 -0.020817716078575188 1107 52-53 -0.04941614481729317 1107 54-55 0.03188653992748414 1107 56-57 0.1397378605070685 1107 58-59 0.12306995425029044 1107 60-61 0.12365794423567422 1107 62-63 0.04323000865156246 1107 64-65 0.021427454148756908 1107 66-67 0.11391168158749565 1107 68-69 0.07398027500786952 1107 70-71 -0.07151138535494539 1107 72-73 0.02676445267781702 1107 74-75 -0.00809826413829029 1107 76-77 -0.055382702439089826 1107 78-79 -0.11532266326835128 1107 80-81 -0.1410153989600147 1107 82-83 -0.09257191691157729 1107 84-85 -0.23964009876367953 1107 86-87 -0.1876668930509915 1107 88-89 -0.18792047792766553 1107 90-91 -0.19841410151731154 1107 92-93 -0.3372463799848262 1107 94-95 -0.31272631333519385 1107 96-97 -0.2211457731476969 1107 98-99 -0.3233216423908942 1107 100-101 -0.3392051726091587 1108 1 0.28269577637746224 1108 2 1.3900886684327425 1108 3 0.6492678538070216 1108 4 0.2760201430199274 1108 5 0.17920558557925403 1108 6 0.06839048978416429 1108 7 0.04539832665778931 1108 8 0.10620490152388129 1108 9 0.04601096650082326 1108 10-11 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-2.3041687805776476 2316 52-53 -2.355583321389716 2316 54-55 -2.594838142752849 2316 56-57 -2.6025757369628195 2316 58-59 -2.4736363567707897 2316 60-61 -2.5090130780221784 2316 62-63 -2.3623758658892413 2316 64-65 -2.113526218060521 2316 66-67 -2.280233513748726 2316 68-69 -2.1234540897905063 2316 70-71 -2.011183774090462 2316 72-73 -2.08781574359935 2316 74-75 -2.1094304358029454 2316 76-77 -2.1645184685267793 2316 78-79 -2.3210500865475936 2316 80-81 -2.2809366590216698 2316 82-83 -2.47386216408054 2316 84-85 -2.0195375547304337 2316 86-87 -1.9979469082770223 2316 88-89 -2.0550076046858443 2316 90-91 -1.8119300554702953 2316 92-93 -2.1008422760754506 2316 94-95 -1.9722468713939918 2316 96-97 -1.7724351152373643 2316 98-99 -1.6313861340528533 2316 100-101 -1.711851304990546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44045.0 3 11825.0 4 9067.0 5 7059.0 6 6328.0 7 6037.0 8 6565.0 9 7640.0 10 8473.0 11 9681.0 12 10814.0 13 11720.0 14 12522.0 15 13712.0 16 15779.0 17 18444.0 18 17557.0 19 16163.0 20 16747.0 21 18219.0 22 19460.0 23 21468.0 24 24266.0 25 28076.0 26 32811.0 27 38952.0 28 46855.0 29 56983.0 30 70459.0 31 88298.0 32 113554.0 33 151352.0 34 218407.0 35 339187.0 36 634847.0 37 1296722.0 38 1376862.0 39 182591.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21300774684389 18.01006879980346 13.707709193247652 25.069214260105007 2 16.99178109552105 20.275947470914264 37.53612622959005 25.196145203974634 3 18.458502689626616 23.979273166632613 28.910005618743956 28.65221852499682 4 13.432750557631437 16.579455713367754 35.94076672799431 34.047027001006505 5 15.252315040909824 35.953327864441796 33.798428802242846 14.99592829240553 6 33.28652411616325 34.69445406604332 17.939662426619364 14.079359391174059 7 29.826446127545157 30.626805170101 21.322444132686826 18.224304569667012 8 27.18038078551287 34.031177901950244 20.152116153876253 18.63632515866063 9 26.90100238518645 16.30504913707675 19.506225680001723 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24.480568976352718 25.70886185261743 25.729018422945543 24.0815507480843 36-37 24.25440387351187 25.621044750043676 25.848104425087975 24.27644695135648 38-39 24.218058593674353 25.810409940908823 25.97331544034694 23.99821602506988 40-41 24.395929766014152 25.60185921360144 25.96219065304359 24.040020367340823 42-43 24.394277184382382 25.518338935657553 25.894666588672678 24.192717291287387 44-45 24.287068580302794 25.704695266318268 25.733606660482394 24.274629492896544 46-47 24.320987222981163 25.74881496007774 25.636003451926335 24.294194365014757 48-49 24.43353124106887 25.81469610928971 25.56467691812565 24.187095731515765 50-51 24.150644489087984 25.82019191175553 25.750105439163473 24.27905815999301 52-53 24.542948747831247 25.71189113362446 25.49468903485266 24.250471083691636 54-55 24.38501537898257 25.563957582076974 25.732320857861147 24.31870618107931 56-57 24.282026009194396 25.842851184915872 25.84319057936829 24.03193222652144 58-59 24.444750341913625 25.577664577968136 25.69436590432647 24.283219175791768 60-61 24.408269891997083 25.545127989870014 25.917306071152456 24.129296046980446 62-63 24.511672431761024 25.64518758461331 25.773361061159385 24.069778922466277 64-65 24.313166313402498 25.572349343305987 25.773406949639366 24.341077393652146 66-67 24.212171152951356 25.782542996327656 25.676905843675186 24.328380007045805 68-69 24.120184665105064 25.57108341647017 25.93760429542144 24.371127623003332 70-71 24.110849417538525 25.62238519255175 25.81896650261265 24.447798887297076 72-73 24.327322317630415 25.52391477755448 25.74289105795274 24.40587184686236 74-75 24.14905567121482 25.645843006275555 25.75264227337857 24.452459049131058 76-77 24.285302871650195 25.525326829529384 25.81710128435083 24.37226901446959 78-79 24.169557129419278 25.681647149906713 25.77459392821601 24.374201792458 80-81 24.145299102637182 25.725572095001265 25.85911333885055 24.270015463511005 82-83 24.234382786445355 25.650807075043762 25.68101448997585 24.433795648535032 84-85 24.13564936960436 25.52474402219293 25.75740120380749 24.582205404395218 86-87 24.231711706135375 25.506906795080358 25.936604142030443 24.324777356753824 88-89 24.107672679208214 25.44673769372377 25.861998394703317 24.5835912323647 90-91 24.034682474496257 25.594912619424548 25.96720940938181 24.40319549669739 92-93 24.188909949095017 25.499765481772936 25.95161624849326 24.35970832063879 94-95 24.046461693854493 25.438512551361736 25.89529437805753 24.619731376726243 96-97 24.259790553299656 25.370911432613752 25.82888252934474 24.540415484741857 98-99 24.03619627053651 25.260971430038616 25.853222996037033 24.849609303387833 100-101 23.934026766040333 25.234969761668424 26.085556752797856 24.745446719493387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 96.5 2 135.0 3 197.0 4 148.0 5 130.5 6 235.5 7 414.5 8 682.0 9 853.0 10 827.0 11 821.5 12 900.5 13 976.5 14 1044.0 15 1102.0 16 1203.5 17 1304.5 18 1357.0 19 1447.5 20 1586.5 21 1805.5 22 2165.5 23 2607.5 24 3254.5 25 4271.0 26 5693.0 27 8053.5 28 11202.5 29 15224.5 30 21025.0 31 27912.5 32 35244.0 33 44139.5 34 53994.0 35 63751.5 36 74238.5 37 85948.0 38 98933.0 39 108366.0 40 116529.0 41 131157.0 42 146386.0 43 161251.5 44 178787.5 45 192071.0 46 200835.5 47 211795.5 48 226165.0 49 234084.5 50 237072.5 51 236699.0 52 228848.5 53 224381.5 54 220903.5 55 209928.0 56 195615.5 57 179934.5 58 161817.5 59 141148.5 60 116947.0 61 92327.5 62 71961.5 63 55565.5 64 42075.5 65 31379.0 66 22627.5 67 16533.5 68 11766.5 69 7944.0 70 5785.0 71 4181.0 72 3043.5 73 2233.0 74 1673.0 75 1303.0 76 933.0 77 592.5 78 389.0 79 268.5 80 189.0 81 138.0 82 110.5 83 97.5 84 92.0 85 74.5 86 60.5 87 58.5 88 55.0 89 57.0 90 48.5 91 34.5 92 32.0 93 41.0 94 40.5 95 35.5 96 27.5 97 19.5 98 16.5 99 13.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05992545847866389 2 0.056032898717391574 3 0.061881719281764835 4 0.062161185110779255 5 0.06104332179472157 6 0.011418175299732092 7 0.01357405455212906 8 0.015031269231989976 9 0.006088362703528476 10-11 0.011887278655577729 12-13 0.014881555395017962 14-15 0.017326881398894153 16-17 0.031539714988770465 18-19 0.029872900937148734 20-21 0.029882881859613533 22-23 0.018853962536008673 24-25 0.007585501073248593 26-27 0.011677679283816913 28-29 0.01894379083819188 30-31 0.016807873430724513 32-33 0.019782188325235147 34-35 0.025241752913481172 36-37 0.023594900706789042 38-39 0.010340235673533607 40-41 0.01112872854825287 42-43 0.012456191236071373 44-45 0.022976083513971395 46-47 0.015161021224032383 48-49 0.014801708015299555 50-51 0.01486159355008836 52-53 0.01252605769332498 54-55 0.008942906528461497 56-57 0.012695733375226592 58-59 0.012306477399099363 60-61 0.013264645955720238 62-63 0.022167628794322533 64-65 0.015839723951638837 66-67 0.018015565048965405 68-69 0.024902401549677947 70-71 0.018814038846149468 72-73 0.024992229851861156 74-75 0.040562468896950366 76-77 0.048627054248509397 78-79 0.03697931773208689 80-81 0.019802150170164748 82-83 0.01702745372495013 84-85 0.02225745709650574 86-87 0.025241752913481172 88-89 0.022696617684956972 90-91 0.02637957807446846 92-93 0.04957524188266547 94-95 0.07755176755150206 96-97 0.09294234999222487 98-99 0.09666523407159555 100-101 0.08129461347580234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5009557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.4095181269046 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41158381954396 36.99047952017704 2 11.385697376768853 11.023522470962838 3 4.165940166206592 6.050134679747339 4 2.203507505666316 4.2668294615329545 5 1.3000213542674643 3.1466703657386943 6 0.8840337774609093 2.56773895048739 7 0.6012997303947921 2.037604113877468 8 0.45330122329910966 1.7555275028997022 9 0.3498519547189882 1.524254808933168 >10 2.03186802432858 17.928045280443985 >50 0.1379073673522746 4.578905289196515 >100 0.07056310013434466 6.150500599155553 >500 0.003172355590385293 1.0551216295912844 >1k 0.0012522442673512078 0.9246653272561831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.996184492960156E-5 2 0.0 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 3 0.0 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 4 0.0 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 5 0.0 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 6 1.996184492960156E-5 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 7 1.996184492960156E-5 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 8 3.992368985920312E-5 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 9 3.992368985920312E-5 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 10-11 3.992368985920312E-5 3.992368985920312E-5 0.0 0.0 1.996184492960156E-5 12-13 6.986645725360546E-5 3.992368985920312E-5 0.0 0.0 3.992368985920312E-5 14-15 9.98092246480078E-5 4.9904612324003895E-5 0.0 0.0 3.992368985920312E-5 16-17 9.98092246480078E-5 5.988553478880468E-5 0.0 3.992368985920312E-5 3.992368985920312E-5 18-19 1.1977106957760937E-4 5.988553478880468E-5 0.0 6.986645725360546E-5 3.992368985920312E-5 20-21 1.3973291450721092E-4 5.988553478880468E-5 0.0 8.982830218320703E-5 5.988553478880468E-5 22-23 1.3973291450721092E-4 5.988553478880468E-5 0.0 1.5969475943681247E-4 5.988553478880468E-5 24-25 1.5969475943681247E-4 5.988553478880468E-5 0.0 2.2956121669041792E-4 5.988553478880468E-5 26-27 1.5969475943681247E-4 5.988553478880468E-5 0.0 2.9942767394402337E-4 5.988553478880468E-5 28-29 2.395421391552187E-4 5.988553478880468E-5 0.0 5.988553478880467E-4 5.988553478880468E-5 30-31 2.5950398408482026E-4 5.988553478880468E-5 0.0 0.001866432500917746 5.988553478880468E-5 32-33 2.5950398408482026E-4 5.988553478880468E-5 0.0 0.004940556620076386 7.984737971840624E-5 34-35 3.1938951887362495E-4 5.988553478880468E-5 0.0 0.008553650552334268 9.98092246480078E-5 36-37 3.5931320873282805E-4 5.988553478880468E-5 0.0 0.015151040301567583 1.1977106957760937E-4 38-39 3.5931320873282805E-4 5.988553478880468E-5 0.0 0.0255910851997492 1.5969475943681247E-4 40-41 3.5931320873282805E-4 5.988553478880468E-5 0.0 0.037857638908989355 1.5969475943681247E-4 42-43 3.5931320873282805E-4 5.988553478880468E-5 0.0 0.05145165530604802 1.5969475943681247E-4 44-45 3.7927505366242963E-4 5.988553478880468E-5 0.0 0.06790021552803971 1.5969475943681247E-4 46-47 4.09217821056832E-4 5.988553478880468E-5 0.0 0.08796186968228928 1.5969475943681247E-4 48-49 4.1919874352163274E-4 5.988553478880468E-5 0.0 0.1088319785561877 1.5969475943681247E-4 50-51 4.391605884512343E-4 5.988553478880468E-5 0.0 0.13191785221727192 1.5969475943681247E-4 52-53 4.391605884512343E-4 5.988553478880468E-5 0.0 0.15591198982265297 1.5969475943681247E-4 54-55 4.591224333808359E-4 5.988553478880468E-5 0.0 0.18212189221521982 1.5969475943681247E-4 56-57 4.99046123240039E-4 5.988553478880468E-5 0.0 0.21398099672286391 1.6967568190161324E-4 58-59 4.99046123240039E-4 5.988553478880468E-5 0.0 0.2497626037591747 1.7965660436641403E-4 60-61 5.090270457048398E-4 5.988553478880468E-5 0.0 0.2893569231770394 1.7965660436641403E-4 62-63 5.190079681696405E-4 5.988553478880468E-5 0.0 0.3317958054973723 1.7965660436641403E-4 64-65 5.190079681696405E-4 5.988553478880468E-5 0.0 0.3800835083820785 1.7965660436641403E-4 66-67 5.988553478880467E-4 5.988553478880468E-5 0.0 0.4367551861372173 1.7965660436641403E-4 68-69 5.988553478880467E-4 5.988553478880468E-5 0.0 0.5002039102459559 1.7965660436641403E-4 70-71 6.088362703528475E-4 5.988553478880468E-5 0.0 0.5731544725411848 1.7965660436641403E-4 72-73 6.287981152824491E-4 5.988553478880468E-5 0.0 0.653051756871915 1.9961844929601558E-4 74-75 6.387790377472499E-4 5.988553478880468E-5 0.0 0.7412930923832187 2.1958029422561716E-4 76-77 6.387790377472499E-4 5.988553478880468E-5 1.996184492960156E-5 0.8454939229157389 2.1958029422561716E-4 78-79 6.387790377472499E-4 5.988553478880468E-5 1.996184492960156E-5 0.9622307920640488 2.1958029422561716E-4 80-81 6.387790377472499E-4 5.988553478880468E-5 1.996184492960156E-5 1.0911443866194157 2.1958029422561716E-4 82-83 6.387790377472499E-4 5.988553478880468E-5 1.996184492960156E-5 1.2363468466373373 2.5950398408482026E-4 84-85 6.487599602120507E-4 5.988553478880468E-5 1.996184492960156E-5 1.3976784773583772 2.5950398408482026E-4 86-87 6.587408826768514E-4 5.988553478880468E-5 1.996184492960156E-5 1.57238454418225 2.5950398408482026E-4 88-89 6.587408826768514E-4 5.988553478880468E-5 1.996184492960156E-5 1.7695077628620655 2.69484906549621E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5815 0.0 36.77146 1 ATCAACG 6445 0.0 33.39689 3 TCAACGC 6500 0.0 33.186737 4 GGTATCA 3710 0.0 32.916023 1 CAACGCA 6695 0.0 32.218204 5 AACGCAG 7160 0.0 30.312477 6 TATCAAC 6905 0.0 30.072247 2 ACGCAGA 8260 0.0 26.161238 7 GTACATG 12060 0.0 26.043674 1 TACATGG 12660 0.0 24.208847 2 ACATGGG 13255 0.0 23.623074 3 CGCAGAG 9230 0.0 23.20492 8 CATGGGG 11095 0.0 21.112598 4 GTACCTG 4595 0.0 20.475183 1 GCAGAGT 11795 0.0 18.159569 9 CCTATAA 2670 0.0 17.796593 1 AGAGTAC 10575 0.0 16.888235 10-11 TACCTGG 5510 0.0 16.38514 2 TATAAGC 2720 0.0 16.245937 3 TATAGGA 2725 0.0 16.041443 4 >>END_MODULE