FastQCFastQC Report
Fri 27 May 2016
SRR940235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5009557
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61500.041.104251
GGTATCA38000.035.7628971
ATCAACG68800.034.7306373
TCAACGC70350.034.1001364
CAACGCA74000.032.2888155
TATCAAC77000.031.5882422
AACGCAG78300.030.6948556
ACGCAGA91050.026.3443957
CGCAGAG96650.024.9162648
GTACATG119750.024.8398531
TACATGG127150.023.0896992
ACATGGG130550.021.577993
GTACCTG49100.021.097221
TATCACG4400.020.5138452
GCAGAGT124200.019.3511059
CATGGGG110350.019.1565154
TACCTGG54000.018.5624312
ATCACGC5700.018.3350033
AGAGTAC113850.017.87699710-11
ATAACGC2606.6260454E-616.4438063
Produced by FastQC (version 0.11.5)