FastQCFastQC Report
Fri 27 May 2016
SRR940234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097233
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58100.037.9219931
ATCAACG64750.034.618473
TCAACGC66950.033.55154
GGTATCA36250.032.972051
CAACGCA69900.032.3383875
AACGCAG75550.030.4841826
TATCAAC70450.030.2518732
ACGCAGA86500.026.6265077
GTACATG118950.025.7057111
CGCAGAG94350.024.3112138
ACATGGG132350.023.933573
TACATGG126050.023.6863332
CATGGGG115900.021.38894
TATCACG4050.021.333782
GTACCTG44850.018.4085941
GCAGAGT123500.018.3413569
ATCACGC5100.017.6924933
AGAGTAC111750.017.12561210-11
TATAAGC26750.017.0432763
ATGGGGA78300.016.8605465
Produced by FastQC (version 0.11.5)