FastQCFastQC Report
Fri 27 May 2016
SRR940234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097233
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66450.037.7507061
ATCAACG69750.034.4603773
TCAACGC70150.034.1284524
GGTATCA41500.033.428811
CAACGCA73800.032.5032965
AACGCAG79600.030.791376
TATCAAC80550.030.0169092
ACGCAGA92100.026.6120437
GTACATG119650.025.8099291
CGCAGAG99250.024.694428
TACATGG126050.024.0054742
ACATGGG128750.022.5796853
CATGGGG112100.019.8738024
GCAGAGT125350.019.4010499
GTACCTG43150.019.26831
TATAAGC27650.018.2106063
TATAACG2905.2206815E-818.0180742
GCTTATA25700.017.5621071
CTATAAG27750.017.4602682
CCTATAA29000.017.2019391
Produced by FastQC (version 0.11.5)