FastQCFastQC Report
Fri 27 May 2016
SRR940233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768300
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61700.038.4964681
ATCAACG64050.035.8935743
TCAACGC65650.034.9453354
GGTATCA37400.034.5488321
CAACGCA68100.033.687425
TATCAAC71900.031.9103452
AACGCAG73750.031.3658756
ACGCAGA84000.027.538787
GTACATG113100.025.7054311
CGCAGAG90300.025.5111968
TACATGG122250.023.3139382
ACATGGG130550.021.9760973
GTATAAC8650.020.3199041
CATGGGG110050.019.9833264
GCAGAGT116300.019.8081269
TATAACG3203.965397E-1019.297752
CCTATAA24200.019.0411191
GTACCTG44450.018.2751271
ATAACGC2603.2857133E-718.26913
AGAGTAC105050.017.54344210-11
Produced by FastQC (version 0.11.5)