##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940233_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4768300 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.167359016840386 34.0 31.0 34.0 30.0 34.0 2 31.787822284671687 34.0 31.0 34.0 30.0 34.0 3 32.19430992177506 34.0 31.0 34.0 30.0 34.0 4 35.69365203531657 37.0 35.0 37.0 35.0 37.0 5 35.76099196778726 37.0 35.0 37.0 35.0 37.0 6 35.83550951072709 37.0 37.0 37.0 35.0 37.0 7 35.817664576473796 37.0 37.0 37.0 35.0 37.0 8 35.81882767443324 37.0 37.0 37.0 35.0 37.0 9 37.575547679466474 39.0 39.0 39.0 35.0 39.0 10-11 37.51887957972443 39.0 38.0 39.0 35.0 39.0 12-13 37.43415609336661 39.0 38.0 39.0 35.0 39.0 14-15 38.87854434494474 41.0 39.0 41.0 36.0 41.0 16-17 38.812996770337435 41.0 39.0 41.0 36.0 41.0 18-19 38.73000387978944 41.0 39.0 41.0 35.5 41.0 20-21 38.65335622758636 41.0 39.0 41.0 35.0 41.0 22-23 38.54947717215779 40.0 39.0 41.0 35.0 41.0 24-25 38.446068829561895 40.0 38.5 41.0 34.0 41.0 26-27 38.29127435354319 40.0 38.0 41.0 34.0 41.0 28-29 38.13386993268041 40.0 38.0 41.0 34.0 41.0 30-31 37.99174443722082 40.0 38.0 41.0 33.5 41.0 32-33 38.06949877314766 40.0 38.0 41.0 34.0 41.0 34-35 38.142726548245705 40.0 38.0 41.0 34.0 41.0 36-37 38.2784042530881 40.5 38.0 41.0 34.0 41.0 38-39 38.20986924060986 40.5 38.0 41.0 34.0 41.0 40-41 38.090771553803236 40.0 38.0 41.0 34.0 41.0 42-43 37.96712727806556 40.0 38.0 41.0 33.0 41.0 44-45 37.84039070528281 40.0 38.0 41.0 33.0 41.0 46-47 37.65250267390894 40.0 38.0 41.0 33.0 41.0 48-49 37.49044795839188 40.0 38.0 41.0 33.0 41.0 50-51 36.58161787219764 39.0 36.0 40.5 31.0 40.5 52-53 36.58797558878426 39.5 36.5 40.5 31.0 41.0 54-55 36.99293312081874 40.0 37.0 41.0 32.0 41.0 56-57 36.88729683535013 40.0 36.5 41.0 31.5 41.0 58-59 36.64005987458843 39.5 36.0 41.0 31.0 41.0 60-61 36.33977937629763 39.0 35.0 41.0 31.0 41.0 62-63 36.00317702325776 39.0 35.0 41.0 30.5 41.0 64-65 35.631111402386594 38.0 35.0 40.0 30.0 41.0 66-67 35.23533848541409 37.0 35.0 40.0 29.5 41.0 68-69 34.801597005221986 37.0 35.0 39.0 29.0 41.0 70-71 34.38584170459073 36.0 34.0 39.0 29.0 41.0 72-73 33.96635771658663 35.5 34.0 38.5 28.5 40.0 74-75 33.53510496403331 35.0 34.0 37.0 28.0 39.5 76-77 33.0326065893505 35.0 34.0 37.0 26.5 39.0 78-79 32.61560335968794 35.0 33.0 36.0 26.0 38.5 80-81 32.27837740913952 35.0 33.0 36.0 26.0 37.0 82-83 31.933502610993436 35.0 33.0 35.5 25.0 37.0 84-85 31.651429125684206 35.0 33.0 35.0 25.0 36.5 86-87 31.369759872491244 35.0 32.5 35.0 24.0 36.0 88-89 31.09474613593943 35.0 32.0 35.0 23.0 36.0 90-91 30.81670144495942 34.5 32.0 35.0 20.0 35.5 92-93 30.540965018979513 34.0 32.0 35.0 18.5 35.0 94-95 30.21752930813917 34.0 31.0 35.0 16.5 35.0 96-97 29.86099700102762 34.0 31.0 35.0 4.5 35.0 98-99 29.45080353585135 34.0 31.0 35.0 2.0 35.0 100-101 27.616068514984377 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.03890776471927637 1101 2 0.0012625699204029672 1101 3 0.018167924486299114 1101 4 0.05886566269218463 1101 5 0.19122620736300888 1101 6 0.1880165268062015 1101 7 0.20641516297906293 1101 8 0.21083201113972194 1101 9 0.32633699699177754 1101 10-11 0.3523583981892031 1101 12-13 0.37681480229137776 1101 14-15 0.4187516694314084 1101 16-17 0.4570876348375492 1101 18-19 0.44789104370113364 1101 20-21 0.4596554831557711 1101 22-23 0.5350208498018887 1101 24-25 0.50074963022481 1101 26-27 0.5310448868351472 1101 28-29 0.6080866700284489 1101 30-31 0.5904543783983129 1101 32-33 0.5919674962673653 1101 34-35 0.5997743488382099 1101 36-37 0.5693831451457712 1101 38-39 0.6637679661386855 1101 40-41 0.661179615163789 1101 42-43 0.6387721397783324 1101 44-45 0.6179173698326679 1101 46-47 0.6673792773274059 1101 48-49 0.6407923896314216 1101 50-51 0.5581882907579327 1101 52-53 0.7558114945076397 1101 54-55 0.7501564353514212 1101 56-57 0.7836078908366986 1101 58-59 0.8018335837031927 1101 60-61 0.8005573413294158 1101 62-63 0.8424728154821466 1101 64-65 0.7648311584971736 1101 66-67 0.7616442384570234 1101 68-69 0.7646055878077576 1101 70-71 0.738057467399635 1101 72-73 0.7297302049546559 1101 74-75 0.6404074382192633 1101 76-77 0.7597169464935689 1101 78-79 0.8211236508784907 1101 80-81 0.8059859894627337 1101 82-83 0.725497595955023 1101 84-85 0.7649801842577908 1101 86-87 0.6409095850918867 1101 88-89 0.6586425885398661 1101 90-91 0.7318484645157675 1101 92-93 0.6485465567474265 1101 94-95 0.8009741113607198 1101 96-97 0.704925652962892 1101 98-99 0.8173164667872364 1101 100-101 0.944607428082552 1102 1 0.3022311001713689 1102 2 0.7730735106481958 1102 3 0.4127631096126265 1102 4 0.27659170750388284 1102 5 0.2652717738654715 1102 6 0.24484157050554956 1102 7 0.2518763897389533 1102 8 0.2240868180680522 1102 9 0.23146273193536615 1102 10-11 0.24505166848953763 1102 12-13 0.29646411501980907 1102 14-15 0.3131005491390155 1102 16-17 0.3519281322155905 1102 18-19 0.3540864195155393 1102 20-21 0.3713485349593597 1102 22-23 0.3686936807251371 1102 24-25 0.3496521806777011 1102 26-27 0.34235407958900055 1102 28-29 0.36814546551550364 1102 30-31 0.31533718469101757 1102 32-33 0.28692518735270056 1102 34-35 0.290483768034143 1102 36-37 0.3756023569684217 1102 38-39 0.3973777309567339 1102 40-41 0.34754094918572065 1102 42-43 0.20392141278008324 1102 44-45 0.2465930809402508 1102 46-47 0.3542098827620208 1102 48-49 0.2879618636501746 1102 50-51 0.2837380086985064 1102 52-53 0.37275393054768813 1102 54-55 0.41267182944009306 1102 56-57 0.38035377912526513 1102 58-59 0.42094757941271155 1102 60-61 0.44694966216209053 1102 62-63 0.45082088079281135 1102 64-65 0.4159832322298911 1102 66-67 0.33257870929763556 1102 68-69 0.39547619800956824 1102 70-71 0.3445200051399766 1102 72-73 0.3378448760177335 1102 74-75 0.4219424771514113 1102 76-77 0.5082286857276372 1102 78-79 0.4700763562654302 1102 80-81 0.4157324339248305 1102 82-83 0.3611428565620507 1102 84-85 0.2550375893110264 1102 86-87 0.3402665094522881 1102 88-89 0.25757741692060776 1102 90-91 0.32679572734748774 1102 92-93 0.32826112054847556 1102 94-95 0.2602800862534558 1102 96-97 0.1288088366590614 1102 98-99 0.12017728436231678 1102 100-101 0.2698333735775513 1103 1 0.198432648923486 1103 2 0.6107032612921302 1103 3 0.2372080575472566 1103 4 0.1974856431174743 1103 5 0.11604683789230563 1103 6 0.0939184100385404 1103 7 0.10698230002067532 1103 8 0.04287062019763255 1103 9 0.15504877555433438 1103 10-11 0.1089370971574084 1103 12-13 0.10354933032607505 1103 14-15 0.08657839842463488 1103 16-17 0.0445432153954215 1103 18-19 0.08819368555159457 1103 20-21 0.112885917271079 1103 22-23 0.06923733546458521 1103 24-25 0.047832369419246845 1103 26-27 0.13513753131387318 1103 28-29 0.005322900553032639 1103 30-31 0.08093340472316157 1103 32-33 0.09456226714222993 1103 34-35 0.02542253435272812 1103 36-37 0.05819820458090419 1103 38-39 0.034314927792451044 1103 40-41 -0.0015194479273858974 1103 42-43 0.10754647332120726 1103 44-45 0.10565118024237563 1103 46-47 0.13716572076129552 1103 48-49 0.11332024877980729 1103 50-51 0.04079492561406539 1103 52-53 0.19222148050329224 1103 54-55 0.19449584647232854 1103 56-57 0.19622089777120522 1103 58-59 0.2337837890611354 1103 60-61 0.22037350601001293 1103 62-63 0.11807746418178766 1103 64-65 0.2820903826874357 1103 66-67 0.2370905597647237 1103 68-69 0.21069839312077931 1103 70-71 0.15041987561865966 1103 72-73 0.12880303567752804 1103 74-75 0.12622502809768577 1103 76-77 0.22068765994078632 1103 78-79 0.23561260847609589 1103 80-81 0.14896595848251337 1103 82-83 0.07881643989965781 1103 84-85 0.0318060323399898 1103 86-87 0.07426555822275205 1103 88-89 0.0486959188395808 1103 90-91 -0.07442309855824902 1103 92-93 -0.05494756838754 1103 94-95 -0.09102709759343597 1103 96-97 -0.1916051499925402 1103 98-99 -0.2403699661531995 1103 100-101 -0.1351195556957343 1104 1 0.24436356314253516 1104 2 0.7665194165924234 1104 3 0.46106176284552447 1104 4 0.2626775657228677 1104 5 0.24100576738588586 1104 6 0.1874561437750586 1104 7 0.23674914058704388 1104 8 0.07380015789066618 1104 9 0.1568914509811563 1104 10-11 0.14393176880697922 1104 12-13 0.15929914966964276 1104 14-15 0.11679725776806293 1104 16-17 0.19770768082342016 1104 18-19 0.190495122289704 1104 20-21 0.17999058565563075 1104 22-23 0.1169930376692605 1104 24-25 0.12279029694713728 1104 26-27 0.06112015144563543 1104 28-29 0.04871036857587541 1104 30-31 0.08325276476700338 1104 32-33 0.04507670591614499 1104 34-35 0.08079046355145181 1104 36-37 0.12053105058981117 1104 38-39 0.2180068669627815 1104 40-41 0.07690570678217057 1104 42-43 -0.014622127423166376 1104 44-45 -0.038263371047577266 1104 46-47 0.016768268094317307 1104 48-49 -0.026722456029041552 1104 50-51 -0.06294154118054962 1104 52-53 0.08790515128238496 1104 54-55 0.2220381125009041 1104 56-57 0.09584144193227218 1104 58-59 0.07928971291989484 1104 60-61 0.13458679505183113 1104 62-63 0.06706178702759757 1104 64-65 0.06926688506326428 1104 66-67 0.08700425846480186 1104 68-69 0.12794420865876077 1104 70-71 0.0880951215474397 1104 72-73 0.0026241513132063687 1104 74-75 0.09791922834817512 1104 76-77 0.20437178064057804 1104 78-79 0.1679882180220389 1104 80-81 0.07877272525220036 1104 82-83 -0.11139553883104725 1104 84-85 -0.051595910612700635 1104 86-87 0.0519401935576731 1104 88-89 -0.018865350017495786 1104 90-91 -0.07043112827094689 1104 92-93 -0.0955610484439191 1104 94-95 -0.14830015135578023 1104 96-97 -0.2250021682950667 1104 98-99 -0.22873044229626416 1104 100-101 -0.06385462265237507 1105 1 -0.05450673175828058 1105 2 0.47387990022680526 1105 3 0.14052489664813805 1105 4 0.1321536533933596 1105 5 0.016949534668619037 1105 6 0.040081318342778616 1105 7 0.020140212538073854 1105 8 -0.09189669904452558 1105 9 0.0013587881353274156 1105 10-11 0.01816085017696878 1105 12-13 -0.07001137084433395 1105 14-15 -0.21921692980567542 1105 16-17 -0.12142912461978028 1105 18-19 -0.3406686716823799 1105 20-21 -0.13127995696555672 1105 22-23 -0.20016694012910108 1105 24-25 -0.05414662934870762 1105 26-27 -0.056686759116985286 1105 28-29 0.029425572634586672 1105 30-31 -0.04232349394455781 1105 32-33 0.08335249377626752 1105 34-35 -0.01611535737462333 1105 36-37 7.661751164178554E-4 1105 38-39 -0.09243043678446128 1105 40-41 -0.16234938701938972 1105 42-43 0.003772688441792127 1105 44-45 0.013327889635718293 1105 46-47 0.05326516680292315 1105 48-49 -0.04811506960969325 1105 50-51 -0.12549320878831338 1105 52-53 0.14045264111148015 1105 54-55 0.016109841511564582 1105 56-57 0.1656122253975667 1105 58-59 0.09097232431614799 1105 60-61 0.04610004838473003 1105 62-63 0.11347515070858805 1105 64-65 0.17648074208670295 1105 66-67 0.045214821919373094 1105 68-69 -0.02251083786137542 1105 70-71 -0.021169462198358246 1105 72-73 -0.15075245512837654 1105 74-75 -0.03162288195156293 1105 76-77 -0.05341043045041971 1105 78-79 0.05123152241808526 1105 80-81 0.03151042925223635 1105 82-83 -0.09405981193406276 1105 84-85 -0.129746687724559 1105 86-87 -0.04587269974836161 1105 88-89 -0.09195221524660369 1105 90-91 -0.31023453093331455 1105 92-93 -0.2330406322117895 1105 94-95 -0.2717571174159268 1105 96-97 -0.30653337466679886 1105 98-99 -0.45864511672962394 1105 100-101 -0.27623045154713566 1106 1 -0.009345571128932306 1106 2 0.5106821114794116 1106 3 0.0592217473238037 1106 4 4.386769644426636E-4 1106 5 -0.00201437558345674 1106 6 0.001830322119047878 1106 7 0.024752423441562144 1106 8 -0.01929057026369918 1106 9 -0.0401607130780377 1106 10-11 -0.13829907052803492 1106 12-13 -0.06014772521905343 1106 14-15 -0.06154448128694412 1106 16-17 -0.05033076507373835 1106 18-19 -0.08350622364252303 1106 20-21 -0.11594663057490351 1106 22-23 -0.1868922584371262 1106 24-25 -0.19877173313668806 1106 26-27 -0.3380077872459992 1106 28-29 -0.23027960079749477 1106 30-31 -0.11372829850731847 1106 32-33 -0.13907571336930147 1106 34-35 -0.21045414420574815 1106 36-37 -0.24198379673588732 1106 38-39 -0.21998217840368284 1106 40-41 -0.3097481169712424 1106 42-43 -0.22533179471222553 1106 44-45 -0.13882754559345756 1106 46-47 -0.08562862558562045 1106 48-49 -0.12352322833694274 1106 50-51 -0.20220235038460288 1106 52-53 -0.039798552466251635 1106 54-55 0.04944721809808783 1106 56-57 -0.0371973120249649 1106 58-59 -0.07617520778993736 1106 60-61 -0.06028479329117431 1106 62-63 -0.051102063149656374 1106 64-65 -0.11197336999141783 1106 66-67 -0.02917043785338791 1106 68-69 -0.38698286169340435 1106 70-71 -0.22594686318439017 1106 72-73 -0.1496780409739884 1106 74-75 -0.053538203425617326 1106 76-77 -0.013904119347607491 1106 78-79 0.0625823226621165 1106 80-81 -0.08538455886938578 1106 82-83 -0.17113690095224143 1106 84-85 -0.24921974058017682 1106 86-87 -0.24803620583445607 1106 88-89 -0.271560983978695 1106 90-91 -0.24596031351834213 1106 92-93 -0.31623676240021936 1106 94-95 -0.3255077440703822 1106 96-97 -0.37422165845434563 1106 98-99 -0.45198894317282523 1106 100-101 -0.4032382746018577 1107 1 0.08082988565143268 1107 2 0.5037792567303327 1107 3 0.15641072478142348 1107 4 0.07376592097255497 1107 5 0.08186607930209533 1107 6 0.03678132140538537 1107 7 0.027210826548412115 1107 8 -0.02520714866754048 1107 9 -0.06034347920800087 1107 10-11 -0.0129191624941285 1107 12-13 -0.02157821999832521 1107 14-15 -0.048142027634376916 1107 16-17 -0.05532800652304104 1107 18-19 -0.031793654908184976 1107 20-21 -0.15200132005710287 1107 22-23 -0.12209087948567543 1107 24-25 -0.09283312951396994 1107 26-27 -0.016856073435647545 1107 28-29 0.01164448561478082 1107 30-31 -0.07218700823977287 1107 32-33 -0.062167751792507886 1107 34-35 -0.11593869362267384 1107 36-37 -0.04735616869000836 1107 38-39 -0.04765832347213461 1107 40-41 0.047462604991679314 1107 42-43 0.058534408561108364 1107 44-45 -0.0537608059412662 1107 46-47 -0.06910566267543317 1107 48-49 0.0013897234634541178 1107 50-51 -0.179778464250667 1107 52-53 -0.005008153734216592 1107 54-55 0.004698235414878127 1107 56-57 0.0338431960234189 1107 58-59 0.06606397960904076 1107 60-61 -0.023726438877332612 1107 62-63 -0.07980151716176209 1107 64-65 -0.03039017782462139 1107 66-67 -0.063420732872558 1107 68-69 0.09312291334057221 1107 70-71 0.043059335187258796 1107 72-73 0.021344274675463737 1107 74-75 0.07267844699205028 1107 76-77 0.184498836350393 1107 78-79 0.12436144210682443 1107 80-81 0.05588832603480398 1107 82-83 0.036136873598049846 1107 84-85 -0.0971221083991729 1107 86-87 -0.03225465065966304 1107 88-89 -0.02411007916326824 1107 90-91 0.018408982462808865 1107 92-93 -0.056630959834713224 1107 94-95 0.0020423498067181356 1107 96-97 -0.06409847917878153 1107 98-99 -0.04668172915787849 1107 100-101 0.07709962896439393 1108 1 0.006194057986604662 1108 2 0.11334591155941709 1108 3 0.019536096988382212 1108 4 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-1.1149888756405062 2316 88-89 -0.988758316935467 2316 90-91 -0.8962255443366907 2316 92-93 -1.019708400507504 2316 94-95 -1.0980569683316297 2316 96-97 -0.9456639124251325 2316 98-99 -0.8194312462604323 2316 100-101 -1.1950864176791463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32847.0 3 10603.0 4 7878.0 5 6328.0 6 5655.0 7 5318.0 8 5548.0 9 6410.0 10 7177.0 11 8331.0 12 9228.0 13 10189.0 14 10943.0 15 12145.0 16 13867.0 17 16563.0 18 15846.0 19 14230.0 20 14976.0 21 15897.0 22 16673.0 23 18518.0 24 21062.0 25 24672.0 26 28691.0 27 34236.0 28 41499.0 29 50783.0 30 61924.0 31 77995.0 32 100289.0 33 134603.0 34 194384.0 35 306589.0 36 581965.0 37 1242114.0 38 1401203.0 39 201114.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22009293348857 17.99918184964914 13.697095566254443 25.08362965060784 2 16.3232483779111 19.883399674270997 38.27689231117169 25.51645963664621 3 18.475642681853863 23.950506228258575 28.90703701543712 28.666814074450443 4 13.450279271481753 16.582281773660252 35.917358464759936 34.05008049009806 5 15.21963271932487 35.98663388840862 33.81270032332833 14.981033068938181 6 33.35845098491682 34.63968197360968 17.93165369527366 14.070213346199836 7 29.827332732518038 30.66310373126438 21.275071032143618 18.234492504073966 8 27.18947033660083 34.01997754540008 20.159527513597787 18.631024604401304 9 26.873033528251533 16.3280667518953 19.52865096104587 37.2702487588073 10-11 25.58344120951654 25.50566338163019 27.477209659595097 21.433685749258167 12-13 26.267179456573736 22.543271237254416 27.594871359721516 23.594677946450325 14-15 23.566669750213805 23.947225615172897 24.770018776075116 27.716085858538186 16-17 24.154103947479946 26.972103546345693 25.366276274083788 23.507516232090573 18-19 24.237476909332894 26.37334198021491 26.31549795117372 23.073683159278474 20-21 24.47459080171842 25.830557926748988 26.23998962677911 23.454861644753482 22-23 24.52517894525283 25.4034443545483 26.12208903536611 23.949287664832763 24-25 24.35861899720458 25.66766768971327 26.005446110718704 23.968267202363442 26-27 24.211200669920263 25.93075567723126 25.827748304563254 24.03029534828522 28-29 24.245439681536066 25.912441298106394 25.943908408053733 23.898210612303806 30-31 24.24815302893186 25.72197489654906 25.91530427770624 24.114567796812832 32-33 24.141672904442864 25.707256827427866 25.926173401227192 24.22489686690208 34-35 24.496893023458778 25.726015418760518 25.708004416629198 24.0690871411515 36-37 24.269840274183714 25.63545121384026 25.85156368169297 24.243144830283057 38-39 24.218797851070057 25.819923497020174 25.982538475931094 23.97874017597867 40-41 24.396959021886527 25.581300228943824 25.96660335665421 24.055137392515434 42-43 24.33162648628099 25.522088069112886 25.92601565265269 24.220269791953434 44-45 24.26214480684467 25.716810400207667 25.74495053451047 24.27609425843719 46-47 24.297703875997936 25.736979097294604 25.674986035567297 24.290330991140163 48-49 24.46300827380335 25.806826142101503 25.543877210368898 24.18628837372625 50-51 24.155441016644595 25.873664527505312 25.71322218196975 24.25767227388034 52-53 24.559905044590067 25.727684146554058 25.43990615223536 24.27250465662052 54-55 24.342124585156686 25.57447669954645 25.718858955371044 24.364539759925822 56-57 24.27008874602253 25.862335361469196 25.8271956917716 24.040380200736674 58-59 24.45665319641126 25.565615416131454 25.70424093820612 24.273490449251163 60-61 24.399711002849063 25.5574722734967 25.888677777197376 24.154138946456857 62-63 24.458021948604486 25.65107700137909 25.810699973534284 24.080201076482137 64-65 24.323876974328744 25.598754722760088 25.72090139946841 24.356466903442765 66-67 24.176414073185263 25.793661948194703 25.66207196019366 24.367852018426376 68-69 24.089812660592145 25.567475393439608 25.948942370482154 24.393769575486097 70-71 24.10674631454773 25.640056836598134 25.804805768079742 24.44839108077439 72-73 24.298860763449788 25.538307256374477 25.75060970225219 24.412222277923547 74-75 24.12838637454693 25.650528884291134 25.737280403334257 24.48380433782768 76-77 24.331945631635065 25.503382811382092 25.78011537184908 24.384556185133757 78-79 24.15119647316194 25.673855585375215 25.757713542268135 24.41723439919471 80-81 24.083179078903868 25.73018424943403 25.85264030999686 24.333996361665243 82-83 24.210762147462013 25.6556948557207 25.704866201077238 24.42867679574005 84-85 24.138434853603492 25.544822239141656 25.73081829769921 24.585924609555644 86-87 24.205795085913138 25.475199352255935 25.959476141182197 24.35952942064873 88-89 24.09521437130773 25.41909059682042 25.84940123921326 24.63629379265859 90-91 24.061165195784046 25.61963023661727 25.89625321905247 24.422951348546214 92-93 24.167667740959597 25.50122247849911 25.936076144025467 24.395033636515834 94-95 24.063966303259125 25.446936710003094 25.852300779509964 24.63679620722782 96-97 24.24076608535668 25.40963038734444 25.792223894022353 24.557379633276526 98-99 24.048055749152322 25.321830939280904 25.79642958003745 24.83368373152932 100-101 23.91091692589532 25.313915837906325 26.057373237527614 24.717793998670736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 339.0 1 220.0 2 150.5 3 188.0 4 146.5 5 133.0 6 204.5 7 415.5 8 691.5 9 800.0 10 796.5 11 817.5 12 854.5 13 925.0 14 996.5 15 1055.5 16 1099.5 17 1180.0 18 1252.5 19 1338.5 20 1491.5 21 1677.5 22 2033.0 23 2537.5 24 3098.0 25 3965.5 26 5411.0 27 7638.5 28 10608.0 29 14565.5 30 20248.5 31 26815.0 32 33515.0 33 41686.0 34 50927.0 35 60504.0 36 70588.0 37 81484.5 38 94177.0 39 103752.0 40 111243.0 41 124755.0 42 139317.0 43 153639.5 44 170334.0 45 182194.0 46 190714.5 47 201619.0 48 214832.0 49 223083.0 50 225477.0 51 224594.5 52 217146.0 53 212788.5 54 210978.0 55 200692.5 56 187040.5 57 172077.0 58 154424.0 59 134354.5 60 110998.0 61 88005.5 62 68954.5 63 53100.5 64 40049.0 65 29948.5 66 21508.5 67 15676.5 68 11042.5 69 7435.0 70 5431.5 71 3930.5 72 2867.5 73 2143.5 74 1619.0 75 1209.5 76 839.5 77 549.0 78 369.5 79 239.0 80 157.0 81 124.0 82 103.0 83 80.5 84 72.0 85 62.5 86 46.5 87 41.5 88 36.5 89 35.0 90 26.5 91 19.0 92 16.5 93 21.0 94 22.0 95 16.0 96 14.0 97 10.0 98 10.0 99 6.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03040916049745192 2 0.03693140112828471 3 0.03099637187257513 4 0.026298680871589455 5 0.027661850135268333 6 0.029360568756160478 7 0.03028332948849695 8 0.028270033345217375 9 0.03175135792630497 10-11 0.018339869555187384 12-13 0.027378730365119643 14-15 0.022293060419856132 16-17 0.038084852043705306 18-19 0.042667197953148925 20-21 0.04575005767254577 22-23 0.03541094310341212 24-25 0.014208418094499088 26-27 0.024442673489503595 28-29 0.029738061783025394 30-31 0.027389216282532557 32-33 0.03447769645366273 34-35 0.037099175806891344 36-37 0.032904808841725565 38-39 0.018958538682549337 40-41 0.015833735293500826 42-43 0.015466728184048822 44-45 0.02280687037308894 46-47 0.017427594740263827 48-49 0.02148564477906172 50-51 0.02447413124174234 52-53 0.030010695635761173 54-55 0.030199442149193633 56-57 0.033712224482519974 58-59 0.0314787240735692 60-61 0.03084956902879433 62-63 0.03681605603674265 64-65 0.031248033890485076 66-67 0.025585638487511275 68-69 0.024683849590000632 70-71 0.034666442967095194 72-73 0.01696621437409559 74-75 0.025931673762137453 76-77 0.04500555753622885 78-79 0.027483589539248787 80-81 0.026927835916364322 82-83 0.02717949793427427 84-85 0.026497913302434832 86-87 0.02065725730344148 88-89 0.024379757985026108 90-91 0.020573369964138163 92-93 0.036784598284503915 94-95 0.07230040056204518 96-97 0.08335255751525701 98-99 0.08301700815804375 100-101 0.08400268439485771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4768300.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.499557457022476 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80916564119948 37.252105422413116 2 11.229352345578976 10.892372385791154 3 4.114705740384138 5.986842224235021 4 2.086562400436148 4.047894121104629 5 1.2742793095645113 3.090099129525948 6 0.854283045476016 2.485940978917451 7 0.5972760822658185 2.0277337968689446 8 0.44167960973278625 1.7137012487864427 9 0.3320239142995216 1.449271161780764 >10 2.0460695492536174 18.14017397126307 >50 0.13774346435904206 4.593527092668937 >100 0.07243026117892039 6.326063462441335 >500 0.003082678670031068 1.0319247519295782 >1k 0.001345957600954621 0.9623502522735753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.097183482582891E-5 5 0.0 0.0 0.0 0.0 2.097183482582891E-5 6 0.0 0.0 0.0 0.0 2.097183482582891E-5 7 0.0 0.0 0.0 0.0 2.097183482582891E-5 8 0.0 0.0 0.0 0.0 2.097183482582891E-5 9 0.0 0.0 0.0 0.0 2.097183482582891E-5 10-11 0.0 0.0 0.0 1.0485917412914456E-5 2.097183482582891E-5 12-13 0.0 0.0 0.0 3.145775223874337E-5 4.194366965165782E-5 14-15 0.0 0.0 0.0 4.194366965165782E-5 5.2429587064572284E-5 16-17 0.0 0.0 0.0 8.388733930331565E-5 7.340142189040119E-5 18-19 2.097183482582891E-5 0.0 0.0 8.388733930331565E-5 8.388733930331565E-5 20-21 4.194366965165782E-5 1.0485917412914456E-5 0.0 8.388733930331565E-5 1.0485917412914455E-4 22-23 4.194366965165782E-5 2.097183482582891E-5 0.0 1.0485917412914455E-4 1.1534509154205902E-4 24-25 6.291550447748674E-5 2.097183482582891E-5 0.0 1.5728876119371684E-4 1.3631692636788793E-4 26-27 6.291550447748674E-5 2.097183482582891E-5 0.0 2.2020426567120356E-4 1.4680284378080238E-4 28-29 7.340142189040119E-5 2.097183482582891E-5 0.0 4.928381184069794E-4 1.4680284378080238E-4 30-31 8.388733930331565E-5 2.097183482582891E-5 0.0 0.0019084369691504308 1.4680284378080238E-4 32-33 9.43732567162301E-5 2.097183482582891E-5 0.0 0.005054212193024768 1.4680284378080238E-4 34-35 1.2583100895497348E-4 2.097183482582891E-5 0.0 0.008881572048738545 2.097183482582891E-4 36-37 1.4680284378080238E-4 3.145775223874337E-5 0.0 0.01562401694524254 2.097183482582891E-4 38-39 2.097183482582891E-4 4.194366965165782E-5 0.0 0.026393054128305685 2.097183482582891E-4 40-41 2.2020426567120356E-4 5.2429587064572284E-5 0.0 0.03912295786758384 2.097183482582891E-4 42-43 2.7263385273577586E-4 6.291550447748674E-5 0.0 0.05206257995512027 2.097183482582891E-4 44-45 3.355493572132626E-4 6.291550447748674E-5 0.0 0.06929094226453872 2.097183482582891E-4 46-47 3.774930268649204E-4 6.291550447748674E-5 0.0 0.08858503030430132 2.2020426567120356E-4 48-49 3.774930268649204E-4 6.291550447748674E-5 0.0 0.1082985550405805 2.3069018308411802E-4 50-51 3.774930268649204E-4 6.291550447748674E-5 0.0 0.12995197449824886 2.3069018308411802E-4 52-53 3.8797894427783483E-4 6.291550447748674E-5 0.0 0.15360820418178386 2.3069018308411802E-4 54-55 3.984648616907493E-4 1.5728876119371684E-4 0.0 0.18069332885934192 2.3069018308411802E-4 56-57 4.194366965165782E-4 1.677746786066313E-4 0.0 0.21150095421848458 2.3069018308411802E-4 58-59 4.194366965165782E-4 1.887465134324602E-4 0.0 0.24774028479751692 2.3069018308411802E-4 60-61 4.194366965165782E-4 1.887465134324602E-4 0.0 0.2872197638571399 2.3069018308411802E-4 62-63 4.194366965165782E-4 1.887465134324602E-4 0.0 0.33050563093765073 2.3069018308411802E-4 64-65 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.37858356227586354 2.4117610049703247E-4 66-67 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.4340855231424197 2.5166201790994695E-4 68-69 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.4989828660109473 2.5166201790994695E-4 70-71 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.5721640836356773 2.5166201790994695E-4 72-73 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.6508399219847745 3.145775223874337E-4 74-75 4.4040853134240713E-4 1.887465134324602E-4 0.0 0.7383449027955455 3.145775223874337E-4 76-77 4.6138036616823604E-4 1.887465134324602E-4 0.0 0.8408342595893714 3.145775223874337E-4 78-79 4.6138036616823604E-4 1.887465134324602E-4 0.0 0.9578780697523226 3.145775223874337E-4 80-81 5.242958706457228E-4 1.9923243084537466E-4 0.0 1.0888996078266888 3.355493572132626E-4 82-83 5.347817880586372E-4 2.097183482582891E-4 0.0 1.2339513033995344 3.774930268649204E-4 84-85 5.662395402973807E-4 2.097183482582891E-4 0.0 1.393976889038022 3.774930268649204E-4 86-87 6.081832099490384E-4 2.097183482582891E-4 0.0 1.5705387664366754 3.774930268649204E-4 88-89 6.291550447748674E-4 2.097183482582891E-4 0.0 1.7699389719606569 3.774930268649204E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6170 0.0 38.496468 1 ATCAACG 6405 0.0 35.893574 3 TCAACGC 6565 0.0 34.945335 4 GGTATCA 3740 0.0 34.548832 1 CAACGCA 6810 0.0 33.68742 5 TATCAAC 7190 0.0 31.910345 2 AACGCAG 7375 0.0 31.365875 6 ACGCAGA 8400 0.0 27.53878 7 GTACATG 11310 0.0 25.705431 1 CGCAGAG 9030 0.0 25.511196 8 TACATGG 12225 0.0 23.313938 2 ACATGGG 13055 0.0 21.976097 3 GTATAAC 865 0.0 20.319904 1 CATGGGG 11005 0.0 19.983326 4 GCAGAGT 11630 0.0 19.808126 9 TATAACG 320 3.965397E-10 19.29775 2 CCTATAA 2420 0.0 19.041119 1 GTACCTG 4445 0.0 18.275127 1 ATAACGC 260 3.2857133E-7 18.2691 3 AGAGTAC 10505 0.0 17.543442 10-11 >>END_MODULE