FastQCFastQC Report
Fri 27 May 2016
SRR940233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768300
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57350.039.2699281
GGTATCA34650.035.240741
ATCAACG62350.035.0436253
TCAACGC63200.034.5723084
CAACGCA66250.032.836945
TATCAAC71150.031.0450952
AACGCAG71450.030.7795186
ACGCAGA82450.026.5575987
GTACATG112050.025.8662281
TACATGG116900.024.218472
ATAACGC3400.023.7496743
CGCAGAG92550.023.659368
ACATGGG123650.022.3955673
GTACCTG46100.021.0254041
TATAACG3252.1827873E-1120.4625432
CATGGGG103450.019.789484
TACCTGG48800.019.2734892
GCAGAGT119000.018.400629
AGAGTAC105800.017.30681810-11
TATAGGA27200.017.1137354
Produced by FastQC (version 0.11.5)