FastQCFastQC Report
Fri 27 May 2016
SRR940232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4828486
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56550.038.889071
GGTATCA34550.036.8439251
TCAACGC62400.034.3304984
ATCAACG62500.034.1235733
CAACGCA63350.033.8917125
AACGCAG66850.032.2593886
TATCAAC69000.031.6672552
GTACATG115950.027.9788041
ACGCAGA82600.026.1090437
TACATGG123100.026.0077932
CGCAGAG87850.024.602568
ACATGGG130500.024.5322593
GTACCTG42950.021.4544661
CATGGGG111300.021.295784
GCAGAGT114950.018.6392219
TACCTGG50450.017.7951772
AGAGTAC102350.017.08153510-11
TATAAGC23300.016.3088073
ATGGGGA72850.016.2357165
AGTACAT83600.015.9662052
Produced by FastQC (version 0.11.5)