##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940232_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4828486 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.350740791212814 34.0 31.0 34.0 31.0 34.0 2 32.35940147698471 34.0 31.0 34.0 31.0 34.0 3 32.46953517106604 34.0 31.0 34.0 31.0 34.0 4 35.84302636478598 37.0 37.0 37.0 35.0 37.0 5 35.84600100321302 37.0 37.0 37.0 35.0 37.0 6 35.913074201727 37.0 37.0 37.0 35.0 37.0 7 35.8739892794553 37.0 37.0 37.0 35.0 37.0 8 35.863539420016956 37.0 37.0 37.0 35.0 37.0 9 37.62297125848558 39.0 39.0 39.0 35.0 39.0 10-11 37.55392735942488 39.0 38.0 39.0 35.0 39.0 12-13 37.49412393450038 39.0 38.0 39.0 35.0 39.0 14-15 38.95444017027283 41.0 39.0 41.0 36.0 41.0 16-17 38.870412899612845 41.0 39.0 41.0 36.0 41.0 18-19 38.816187931372276 41.0 39.0 41.0 36.0 41.0 20-21 38.74622480007191 41.0 39.0 41.0 35.5 41.0 22-23 38.635144743093385 40.5 39.0 41.0 35.0 41.0 24-25 38.52282651332115 40.0 39.0 41.0 35.0 41.0 26-27 38.36834827728609 40.0 38.0 41.0 34.0 41.0 28-29 38.21489841743354 40.0 38.0 41.0 34.0 41.0 30-31 38.186539631677505 40.0 38.0 41.0 34.0 41.0 32-33 38.21286900283029 40.0 38.0 41.0 34.0 41.0 34-35 38.2440257670831 40.0 38.0 41.0 34.5 41.0 36-37 38.38858681582592 41.0 39.0 41.0 35.0 41.0 38-39 38.321277000699595 41.0 39.0 41.0 34.0 41.0 40-41 38.213011283454065 40.5 38.0 41.0 34.0 41.0 42-43 38.104761099027726 40.0 38.0 41.0 34.0 41.0 44-45 37.962175721333764 40.0 38.0 41.0 33.0 41.0 46-47 37.80351232249612 40.0 38.0 41.0 33.0 41.0 48-49 37.63572007871619 40.0 38.0 41.0 33.0 41.0 50-51 36.70762243071638 39.0 36.5 40.5 31.5 40.5 52-53 36.79490279147542 39.5 37.0 40.5 31.5 41.0 54-55 37.062353913835516 40.0 37.0 41.0 32.0 41.0 56-57 37.00300684313882 40.0 36.5 41.0 32.0 41.0 58-59 36.77509088770269 39.5 36.0 41.0 31.5 41.0 60-61 36.47378961024222 39.0 35.5 41.0 31.0 41.0 62-63 36.13175703522802 39.0 35.0 41.0 31.0 41.0 64-65 35.74510125948382 38.0 35.0 40.0 30.0 41.0 66-67 35.35417499398362 37.0 35.0 40.0 30.0 41.0 68-69 34.94540586842335 37.0 35.0 39.0 29.0 41.0 70-71 34.524170412837485 36.0 34.5 39.0 29.0 41.0 72-73 34.09775983610598 36.0 34.0 38.5 29.0 40.0 74-75 33.66482568241888 35.0 34.0 37.0 28.5 39.5 76-77 33.23085020853327 35.0 34.0 37.0 27.5 39.0 78-79 32.82845305961331 35.0 34.0 36.5 26.5 38.5 80-81 32.43654543059667 35.0 33.0 36.0 26.0 37.0 82-83 32.08925209682704 35.0 33.0 35.5 26.0 37.0 84-85 31.765310906979952 35.0 33.0 35.0 25.0 36.5 86-87 31.490203968697436 35.0 32.5 35.0 24.5 36.0 88-89 31.20222840037229 35.0 32.0 35.0 23.5 36.0 90-91 30.938868829691128 34.5 32.0 35.0 22.5 35.5 92-93 30.63350427028265 34.0 32.0 35.0 19.5 35.0 94-95 30.302772442542032 34.0 31.0 35.0 17.5 35.0 96-97 29.96396251330127 34.0 31.0 35.0 6.5 35.0 98-99 29.524703084983575 34.0 31.0 35.0 2.0 35.0 100-101 27.57474402949496 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11875971046377742 1101 2 0.1619489978493931 1101 3 0.12150305861140964 1101 4 0.0892735776768987 1101 5 0.05948008891460432 1101 6 -0.0024625522867083305 1101 7 0.03298057629640283 1101 8 0.08357974770881071 1101 9 0.055047056352044876 1101 10-11 0.08592137163208236 1101 12-13 0.07469212690447335 1101 14-15 0.10482579173748974 1101 16-17 0.0892715528974577 1101 18-19 0.13294224452991443 1101 20-21 0.053090510195332286 1101 22-23 0.1373379149098355 1101 24-25 0.10887457571435988 1101 26-27 0.15690430574414904 1101 28-29 0.14828659918647702 1101 30-31 0.1313220948815541 1101 32-33 0.09765811142614211 1101 34-35 0.024177285089294287 1101 36-37 0.07788760002225104 1101 38-39 0.12275316620948473 1101 40-41 0.0976092761888836 1101 42-43 0.12175987444609149 1101 44-45 0.1402811709804297 1101 46-47 0.1410575801510987 1101 48-49 0.004376301880697042 1101 50-51 0.010445190837891971 1101 52-53 0.1292219073616252 1101 54-55 0.203459850834399 1101 56-57 0.17130578236576355 1101 58-59 0.06962370888296476 1101 60-61 0.03519771803163252 1101 62-63 0.06622640078068542 1101 64-65 0.1300695565042318 1101 66-67 0.05817339528213239 1101 68-69 0.03654292554805494 1101 70-71 0.0686438474085378 1101 72-73 -0.025619834944137665 1101 74-75 -0.06716172326148495 1101 76-77 -0.05957745998716746 1101 78-79 0.00879345209025928 1101 80-81 0.027573143223108332 1101 82-83 -0.0455019660222149 1101 84-85 -0.022851602813023675 1101 86-87 0.054954763503101134 1101 88-89 0.10658298023036039 1101 90-91 0.01438128885767398 1101 92-93 0.012435347043069811 1101 94-95 0.012892736753851608 1101 96-97 -0.03511690287009728 1101 98-99 -0.056599472013733276 1101 100-101 0.2541995236105947 1102 1 0.09056105726512698 1102 2 0.14708774160882854 1102 3 0.13819778363946966 1102 4 0.15608150368022677 1102 5 0.17203281928343017 1102 6 0.13755592753062018 1102 7 0.09727050624724853 1102 8 0.18083099582212725 1102 9 0.15112033705176486 1102 10-11 0.18273360314258014 1102 12-13 0.20957181458588536 1102 14-15 0.23463320495007878 1102 16-17 0.2190160766793383 1102 18-19 0.1752559466855672 1102 20-21 0.2493926116698475 1102 22-23 0.2591341821802473 1102 24-25 0.26585989828875967 1102 26-27 0.21359066164027496 1102 28-29 0.3251885207004719 1102 30-31 0.23499290257534255 1102 32-33 0.1669126781663408 1102 34-35 0.2529545210504338 1102 36-37 0.240397517201707 1102 38-39 0.2884994702460162 1102 40-41 0.26593525629646564 1102 42-43 0.275435036023552 1102 44-45 0.21950492189958482 1102 46-47 0.2680676231094168 1102 48-49 0.2111652159253623 1102 50-51 0.1983829980285705 1102 52-53 0.19205207687283377 1102 54-55 0.38058430418012534 1102 56-57 0.30493251908158925 1102 58-59 0.335926584381248 1102 60-61 0.2981565689933632 1102 62-63 0.30553277873648454 1102 64-65 0.24258842333343722 1102 66-67 0.2576852765468871 1102 68-69 0.29003449836434214 1102 70-71 0.24919940296409493 1102 72-73 0.23979170381510073 1102 74-75 0.1495564643618792 1102 76-77 0.20595260116070335 1102 78-79 0.27527048293510603 1102 80-81 0.22111657563148412 1102 82-83 0.16813463949657148 1102 84-85 0.18133102809374435 1102 86-87 0.21266452394274538 1102 88-89 0.12031585495461528 1102 90-91 0.02330554095721382 1102 92-93 0.05204117544200315 1102 94-95 0.061111998335181994 1102 96-97 0.06544813447655073 1102 98-99 0.20298270028395748 1102 100-101 0.4584486721844563 1103 1 0.02468159202614828 1103 2 0.09787801873257962 1103 3 0.05180519330945543 1103 4 0.06279917740756247 1103 5 0.02956905239715013 1103 6 0.05549450057733907 1103 7 0.04834281055873646 1103 8 0.04333377101914948 1103 9 0.023215483532673886 1103 10-11 -0.016913142558863115 1103 12-13 0.04748090867673227 1103 14-15 0.02920423903444913 1103 16-17 -0.045429309917984995 1103 18-19 -0.05832589051606618 1103 20-21 0.012448411159915906 1103 22-23 -0.09151996003993901 1103 24-25 -0.062308431021413924 1103 26-27 -0.014075410038294933 1103 28-29 -0.014106934552039263 1103 30-31 -0.03475271261336843 1103 32-33 -0.05988442099268809 1103 34-35 -0.060301057272340586 1103 36-37 -0.03607861362920772 1103 38-39 -0.07352255067719682 1103 40-41 -0.03052065801959003 1103 42-43 -0.11993782672121256 1103 44-45 -0.08956249523691895 1103 46-47 -0.13641955229859093 1103 48-49 -0.08154581748023304 1103 50-51 -0.09509656167315939 1103 52-53 -0.07912342764738156 1103 54-55 -0.0011246462368319499 1103 56-57 -0.07095302507622137 1103 58-59 0.038159963956076126 1103 60-61 0.05759007350508938 1103 62-63 0.11049573250771516 1103 64-65 0.1168520239200106 1103 66-67 0.14629832499299056 1103 68-69 0.09303523759198384 1103 70-71 0.09548053289705649 1103 72-73 0.10305734885574225 1103 74-75 0.1394977450817123 1103 76-77 0.07761639894535222 1103 78-79 0.08114916334130129 1103 80-81 0.07758705354515882 1103 82-83 -0.03701707186313996 1103 84-85 -0.030315572307582528 1103 86-87 -0.12887729421079186 1103 88-89 -0.2278798515858007 1103 90-91 -0.330435256701584 1103 92-93 -0.24115697574649175 1103 94-95 -0.2089179419751055 1103 96-97 -0.27421406217850475 1103 98-99 -0.41608425549814143 1103 100-101 -0.20735224790825413 1104 1 0.1615114020774726 1104 2 0.1656014274384887 1104 3 0.18289351064501602 1104 4 0.17010027108221948 1104 5 0.19394060605691266 1104 6 0.1780098050385277 1104 7 0.19673306809703917 1104 8 0.16226993238203846 1104 9 0.24604828338682694 1104 10-11 0.25235170076842905 1104 12-13 0.26171223405637534 1104 14-15 0.2029212516738923 1104 16-17 0.19643833683817036 1104 18-19 0.3087480813118546 1104 20-21 0.3214443780521421 1104 22-23 0.2625301601059036 1104 24-25 0.27929647770468335 1104 26-27 0.3266062620777035 1104 28-29 0.3322498342322078 1104 30-31 0.3474374906196829 1104 32-33 0.3111412351806706 1104 34-35 0.3508039572482389 1104 36-37 0.2881232322498022 1104 38-39 0.15263504632669367 1104 40-41 0.16478940850822 1104 42-43 0.28572421894703837 1104 44-45 0.2903501125802279 1104 46-47 0.20048527409124972 1104 48-49 0.20211779671928554 1104 50-51 0.108395138433373 1104 52-53 0.11491000048044953 1104 54-55 0.28683296656087975 1104 56-57 0.15630897674136435 1104 58-59 0.12989663137569352 1104 60-61 0.1682671607630155 1104 62-63 0.1646510529280647 1104 64-65 0.25443809011954244 1104 66-67 0.20349042494841285 1104 68-69 0.21537159135701245 1104 70-71 0.14962676799572705 1104 72-73 0.1222271192433908 1104 74-75 0.04457486396172783 1104 76-77 0.01937018638344057 1104 78-79 0.015362435196429658 1104 80-81 0.09494376402834348 1104 82-83 0.10976768877135612 1104 84-85 0.06419674700108757 1104 86-87 0.08804350167716635 1104 88-89 0.10081627902306423 1104 90-91 0.20032604933537002 1104 92-93 0.1519755716907305 1104 94-95 0.12436149659852092 1104 96-97 0.16307121098008537 1104 98-99 0.15782983111657956 1104 100-101 0.33638597615851396 1105 1 -0.16858722831173623 1105 2 -0.03862310838773908 1105 3 -0.07399454783857351 1105 4 0.008915283656861561 1105 5 0.023848252527436387 1105 6 0.047251351154613985 1105 7 0.12769773774591187 1105 8 0.04546887117429321 1105 9 0.08384949942378483 1105 10-11 -0.004556903969351822 1105 12-13 0.018106910442412527 1105 14-15 0.05691065633467218 1105 16-17 0.06083726475051776 1105 18-19 0.10195241820346723 1105 20-21 0.0074826961885037235 1105 22-23 0.12803982019302396 1105 24-25 0.1372035215966676 1105 26-27 0.19533385891918442 1105 28-29 0.2641213008832466 1105 30-31 0.22927380685624854 1105 32-33 0.29501160965812545 1105 34-35 0.14626036864199676 1105 36-37 0.16185177062024536 1105 38-39 0.17830698416067747 1105 40-41 0.15087375694925953 1105 42-43 0.17591861329284342 1105 44-45 0.1969070039445242 1105 46-47 0.19117265300016584 1105 48-49 0.2918649857322748 1105 50-51 0.1489268721438819 1105 52-53 0.24878702842921285 1105 54-55 0.4554759931789576 1105 56-57 0.369971085013006 1105 58-59 0.3660945909778235 1105 60-61 0.3711057031924909 1105 62-63 0.3940954462277304 1105 64-65 0.39324155327228283 1105 66-67 0.46889241596831965 1105 68-69 0.38306815121942606 1105 70-71 0.4110285790789874 1105 72-73 0.43240207624690186 1105 74-75 0.28530862886404407 1105 76-77 0.3226612694765123 1105 78-79 0.34541237807700753 1105 80-81 0.4247050968985775 1105 82-83 0.4157060165322335 1105 84-85 0.4707301210642676 1105 86-87 0.4795323518820034 1105 88-89 0.5748265228581424 1105 90-91 0.4278560631863044 1105 92-93 0.4883264774415892 1105 94-95 0.4659156470674013 1105 96-97 0.3448406995834006 1105 98-99 0.27237998950267084 1105 100-101 0.4756831770975296 1106 1 -0.10149039038333996 1106 2 0.014789228713297575 1106 3 0.04867152060574398 1106 4 0.15722409810640414 1106 5 0.1293751684168214 1106 6 0.13574144344001837 1106 7 0.09850690207319701 1106 8 0.1234101081773602 1106 9 0.023143734261729776 1106 10-11 -0.011495267858677494 1106 12-13 0.12849799144116503 1106 14-15 0.09689493241702252 1106 16-17 0.06329764624075551 1106 18-19 0.22244751579877686 1106 20-21 0.13921459104406608 1106 22-23 0.21135493244204184 1106 24-25 0.2170107183761516 1106 26-27 0.20449341140496813 1106 28-29 0.19833613405330652 1106 30-31 0.18521687145603494 1106 32-33 0.29901021837955 1106 34-35 0.07604949526481164 1106 36-37 0.18976534670013478 1106 38-39 0.17810282020384705 1106 40-41 0.2530076407569126 1106 42-43 0.29855508619584015 1106 44-45 0.28546937685657525 1106 46-47 0.2434489576325447 1106 48-49 0.22757651358665498 1106 50-51 0.1418636224793488 1106 52-53 0.29270602967230275 1106 54-55 0.42147190989981453 1106 56-57 0.3966188033984679 1106 58-59 0.4098128858794823 1106 60-61 0.5151998079582398 1106 62-63 0.5387542125176239 1106 64-65 0.3673789193925927 1106 66-67 0.461427095027517 1106 68-69 0.4993374665292478 1106 70-71 0.4505929607009378 1106 72-73 0.4577712590148124 1106 74-75 0.5219783689666144 1106 76-77 0.35419340471867145 1106 78-79 0.3515193912735697 1106 80-81 0.5285098321851933 1106 82-83 0.6441514891141651 1106 84-85 0.505425645098807 1106 86-87 0.5357996502495119 1106 88-89 0.593526047847643 1106 90-91 0.4504370907566724 1106 92-93 0.5273300667756686 1106 94-95 0.6104300579144315 1106 96-97 0.643864746475252 1106 98-99 0.7143915395845859 1106 100-101 0.9725257156458298 1107 1 0.02346048075806806 1107 2 0.06394111303458772 1107 3 0.07082920350671884 1107 4 0.12871736921555765 1107 5 0.06422570076131962 1107 6 0.09111120676978146 1107 7 0.11745544371196104 1107 8 0.05527470541792212 1107 9 0.07520120265692753 1107 10-11 0.0753815047340467 1107 12-13 0.10846152394493913 1107 14-15 0.16430729541692557 1107 16-17 0.18196575999614595 1107 18-19 0.2024434556217969 1107 20-21 0.16224870569323713 1107 22-23 0.2576138809055948 1107 24-25 0.2767844750525157 1107 26-27 0.20378710314399484 1107 28-29 0.25200143970552347 1107 30-31 0.23453071269976533 1107 32-33 0.2570485362201822 1107 34-35 0.2548432649646557 1107 36-37 0.22230205620554244 1107 38-39 0.28779639714885974 1107 40-41 0.23572840471394585 1107 42-43 0.13201032708679605 1107 44-45 0.24895817954018185 1107 46-47 0.17902503946136505 1107 48-49 0.18973974337777122 1107 50-51 0.21892525980626942 1107 52-53 0.27579608170488257 1107 54-55 0.4055480646700005 1107 56-57 0.3562217752950474 1107 58-59 0.37275247712260295 1107 60-61 0.36753935348453126 1107 62-63 0.32168220006811765 1107 64-65 0.34095215479513996 1107 66-67 0.2721417196400111 1107 68-69 0.3179456337154889 1107 70-71 0.23199854122935903 1107 72-73 0.35467184451368183 1107 74-75 0.3115694567187859 1107 76-77 0.34347767662400486 1107 78-79 0.30406408090706094 1107 80-81 0.36326276013768677 1107 82-83 0.4387846139597542 1107 84-85 0.3632033260286498 1107 86-87 0.42087536864628206 1107 88-89 0.4418233828306519 1107 90-91 0.4712760327937424 1107 92-93 0.5149646527591187 1107 94-95 0.48176218477393107 1107 96-97 0.6070914422664124 1107 98-99 0.49872596893730403 1107 100-101 0.6703945888200309 1108 1 0.29778364288986836 1108 2 0.32411691019125755 1108 3 0.3178491779201451 1108 4 0.24156049190440143 1108 5 0.2931521148513383 1108 6 0.3255074156639495 1108 7 0.3590468217248812 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4 6533.0 5 5618.0 6 4991.0 7 4787.0 8 5286.0 9 5828.0 10 6976.0 11 7889.0 12 8712.0 13 9652.0 14 10477.0 15 11586.0 16 13513.0 17 16079.0 18 15405.0 19 13402.0 20 13997.0 21 15078.0 22 15989.0 23 17922.0 24 20412.0 25 24019.0 26 27812.0 27 33371.0 28 40692.0 29 49591.0 30 61354.0 31 76842.0 32 99832.0 33 134432.0 34 195482.0 35 307839.0 36 585626.0 37 1257624.0 38 1448930.0 39 212538.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19807150967093 18.0508236001842 13.697571965713243 25.053532924431625 2 15.611180146356563 19.85225536696664 38.663001606433355 25.87356288024344 3 18.5209706073422 23.930402077505402 28.883639147785278 28.664988167367124 4 13.44821264514846 16.547488961414928 35.934921287612234 34.06937710582437 5 15.240975600457729 35.970278733220304 33.80274044467116 14.98600522165081 6 33.29103993898151 34.641894407100814 17.978559424152476 14.0885062297652 7 29.83266783711138 30.611541827861764 21.304041141649705 18.251749193377147 8 27.196332144845385 34.00583145709147 20.14724294485061 18.650593453212533 9 26.869060043511173 16.28729388436436 19.563951934697613 37.27969413742686 10-11 25.582236277864183 25.559531435020645 27.428682762798196 21.429549524316972 12-13 26.26346797170515 22.53358259933032 27.618559220026267 23.584390208938256 14-15 23.539682274955982 23.958004331455925 24.784672857193616 27.717640536394473 16-17 24.17473230760433 26.961536560929567 25.372273699060905 23.491457432405202 18-19 24.217831294745988 26.35563114537816 26.351166064640484 23.075371495235363 20-21 24.49779385341134 25.80937190421844 26.24293541547687 23.44989882689335 22-23 24.473707083966623 25.407232433515453 26.13793279922823 23.98112768328969 24-25 24.345645664618353 25.666886585210495 25.995117601182383 23.992350148988773 26-27 24.24453409224148 25.90587133225585 25.846345130576477 24.003249444926197 28-29 24.26226978137452 25.88432643734832 25.932273437734672 23.921130343542487 30-31 24.27236215517755 25.692861045582728 25.94135849623359 24.093418303006136 32-33 24.114053223207684 25.698500993115626 25.9570197539576 24.23042602971909 34-35 24.48088578586022 25.716533109957258 25.72830137703228 24.07427972715024 36-37 24.262313353255024 25.63756890442674 25.830947222293293 24.269170520024947 38-39 24.218979305693562 25.774775552933214 26.001019521918373 24.005225619454848 40-41 24.38724869534885 25.55200205318074 25.998597863155048 24.062151388315367 42-43 24.329335861483028 25.51808546415082 25.916821085489318 24.235757588876833 44-45 24.251963153939275 25.711723573342038 25.734168200262005 24.302145072456682 46-47 24.30962240394752 25.744735345545333 25.645216699288913 24.300425551218236 48-49 24.439872967681254 25.805954103924666 25.553088900768145 24.201084027625935 50-51 24.146502074320928 25.82880658811286 25.74556653776905 24.279124799797167 52-53 24.561742960190088 25.69564280668463 25.475110778734628 24.26750345439065 54-55 24.39691218708343 25.550926851398227 25.7155818961276 24.336579065390744 56-57 24.27467920868766 25.859891202269715 25.834504858051393 24.030924730991238 58-59 24.471510687240407 25.562715701600574 25.670226646121513 24.295546965037502 60-61 24.381811359236664 25.51789452686315 25.909324840967518 24.190969272932666 62-63 24.447583453376602 25.613194277861535 25.800778717270862 24.138443551491 64-65 24.321981919241654 25.582835480344844 25.749513781591247 24.34566881882225 66-67 24.219481065716742 25.786966229945673 25.62496044881878 24.368592255518806 68-69 24.08598256990258 25.575698456124492 25.903255936895185 24.435063037077747 70-71 24.11121854482178 25.604666826470567 25.816424264033618 24.467690364674027 72-73 24.3218311656458 25.490764540741917 25.73245424323178 24.454950050380507 74-75 24.125864817441155 25.635452871754833 25.717132016805706 24.521550293998306 76-77 24.285484150848593 25.532955719757894 25.75721363656377 24.424346492829745 78-79 24.144849478779157 25.663418356465733 25.746872939077324 24.444859225677785 80-81 24.10109348876298 25.66801584209117 25.849283453822796 24.381607215323054 82-83 24.21488001317393 25.643671155157694 25.699277142873417 24.442171688794964 84-85 24.11306193720045 25.51168398046833 25.741840646315634 24.633413436015587 86-87 24.22770627245582 25.49595186777517 25.91605239527005 24.360289464498962 88-89 24.083259305173904 25.423928608126968 25.87025211560326 24.62255997109587 90-91 24.067773542411857 25.57610908365496 25.898315449436865 24.457801924496323 92-93 24.178571310175187 25.501490963880386 25.924082671833794 24.395855054110633 94-95 24.064478360315384 25.45475893571553 25.83944993156685 24.641312772402234 96-97 24.278344638296463 25.35598195628379 25.774009917410197 24.59166348800955 98-99 24.03762873361419 25.26192320263097 25.833096199509924 24.86735186424491 100-101 23.920065979155794 25.223657979576974 26.086183303161288 24.770092738105948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 251.0 1 187.5 2 182.5 3 218.5 4 166.0 5 149.0 6 222.0 7 425.5 8 664.5 9 776.0 10 758.0 11 746.0 12 810.0 13 888.5 14 960.0 15 1018.5 16 1069.5 17 1132.5 18 1201.5 19 1292.5 20 1417.5 21 1629.5 22 1957.0 23 2399.5 24 3023.5 25 3988.5 26 5344.5 27 7587.0 28 10594.0 29 14414.0 30 20170.5 31 26857.0 32 33767.5 33 42398.5 34 51791.0 35 61196.5 36 71440.5 37 82468.5 38 95246.5 39 104718.0 40 112289.0 41 126371.0 42 141350.5 43 155793.0 44 172642.0 45 185199.0 46 193277.5 47 204261.0 48 218156.5 49 226294.0 50 229127.0 51 228008.5 52 220287.0 53 215299.5 54 213004.5 55 203038.5 56 189518.0 57 174477.5 58 156468.5 59 136252.0 60 112740.5 61 89028.5 62 69448.0 63 53596.0 64 40438.5 65 30350.5 66 21707.5 67 15771.0 68 11214.0 69 7512.0 70 5459.5 71 3968.5 72 2917.0 73 2146.5 74 1576.0 75 1166.0 76 828.5 77 548.5 78 369.5 79 256.0 80 169.5 81 126.5 82 105.0 83 82.0 84 57.0 85 50.5 86 46.0 87 45.5 88 43.5 89 32.5 90 34.0 91 27.5 92 15.5 93 17.0 94 16.0 95 15.5 96 12.5 97 7.5 98 8.0 99 8.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022429390910525576 2 0.034047939664731346 3 0.023982672829537043 4 0.02280217857108833 5 0.04231139947387235 6 0.02271933686874105 7 0.02611584666497946 8 0.03945336074289125 9 0.02526671921591985 10-11 0.02737918262577545 12-13 0.025815545493970575 14-15 0.03525949956156029 16-17 0.04344011766835401 18-19 0.04443421809652135 20-21 0.03846961552751732 22-23 0.04468274320356319 24-25 0.0194885104771972 26-27 0.024852510704183463 28-29 0.02653005517671585 30-31 0.026374726984814703 32-33 0.03638821775604195 34-35 0.04032319861753767 36-37 0.029398449120490357 38-39 0.015180741955138731 40-41 0.017697058663937306 42-43 0.020223730585529294 44-45 0.021745946866160534 46-47 0.015481043126147617 48-49 0.02506997017284507 50-51 0.018266595367574846 52-53 0.026840711560518142 54-55 0.02321638708282472 56-57 0.022522587825666265 58-59 0.022170510590690334 60-61 0.019353892710882874 62-63 0.02195305112202873 64-65 0.019115722816634446 66-67 0.018235529729194618 68-69 0.022553653464046492 70-71 0.03597400924430556 72-73 0.025308140067093497 74-75 0.0346381867939557 76-77 0.043978588733611323 78-79 0.027120302305940206 80-81 0.022636495166393773 82-83 0.015915962063470828 84-85 0.03223577742588463 86-87 0.019695614733065393 88-89 0.030941375826708414 90-91 0.02371343729690839 92-93 0.043036264369411034 94-95 0.07672177158637303 96-97 0.09622063727636364 98-99 0.07254862083062891 100-101 0.08143339340737449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4828486.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.757932403122346 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40762466002772 36.490701735967484 2 11.295416087685375 10.788914359616378 3 4.197503189826314 6.013922208048466 4 2.1786474748959757 4.161907953452607 5 1.299188077716228 3.102326819725704 6 0.8862535763327559 2.5395383034315113 7 0.6024264770742893 2.0139450078975534 8 0.4473892623857331 1.7093108920720488 9 0.3650411423230194 1.5690249179479192 >10 2.095780168218151 18.271002921425236 >50 0.14557702472528647 4.796713992138712 >100 0.07436217532043742 6.441767167603237 >500 0.003484134114794931 1.137850711832845 >1k 0.0013065493539777906 0.9630730088403046 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.0710425586819553E-5 5 0.0 2.0710425586819553E-5 0.0 0.0 2.0710425586819553E-5 6 0.0 2.0710425586819553E-5 0.0 0.0 2.0710425586819553E-5 7 0.0 2.0710425586819553E-5 0.0 0.0 2.0710425586819553E-5 8 0.0 2.0710425586819553E-5 0.0 2.0710425586819553E-5 2.0710425586819553E-5 9 0.0 2.0710425586819553E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 10-11 0.0 2.0710425586819553E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 12-13 0.0 2.0710425586819553E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 14-15 0.0 3.106563838022933E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 16-17 0.0 4.1420851173639106E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 18-19 0.0 4.1420851173639106E-5 0.0 4.1420851173639106E-5 2.0710425586819553E-5 20-21 0.0 5.177606396704888E-5 0.0 4.1420851173639106E-5 4.1420851173639106E-5 22-23 0.0 6.213127676045866E-5 0.0 4.1420851173639106E-5 4.1420851173639106E-5 24-25 2.0710425586819553E-5 6.213127676045866E-5 0.0 4.1420851173639106E-5 6.213127676045866E-5 26-27 2.0710425586819553E-5 6.213127676045866E-5 0.0 1.0355212793409776E-4 8.284170234727821E-5 28-29 1.0355212793409776E-4 6.213127676045866E-5 0.0 4.556293629100302E-4 8.284170234727821E-5 30-31 1.2426255352091732E-4 6.213127676045866E-5 0.0 0.0018432278772269402 8.284170234727821E-5 32-33 1.2426255352091732E-4 6.213127676045866E-5 0.0 0.0046287801186541706 8.284170234727821E-5 34-35 1.5532819190114665E-4 6.213127676045866E-5 0.0 0.008677668320877394 9.319691514068798E-5 36-37 1.86393830281376E-4 7.248648955386844E-5 0.0 0.015657081743635583 1.0355212793409776E-4 38-39 2.278146814550151E-4 8.284170234727821E-5 0.0 0.026177977941739917 1.0355212793409776E-4 40-41 2.278146814550151E-4 9.319691514068798E-5 0.0 0.03941193989171761 1.0355212793409776E-4 42-43 2.278146814550151E-4 1.0355212793409776E-4 0.0 0.05290478216153055 1.0355212793409776E-4 44-45 2.692355326286542E-4 1.0355212793409776E-4 0.0 0.06803374805270224 1.0355212793409776E-4 46-47 3.210115965957031E-4 1.0355212793409776E-4 0.0 0.08692165618788167 1.0355212793409776E-4 48-49 3.3136680938911285E-4 1.0355212793409776E-4 0.0 0.1074042670932462 1.0355212793409776E-4 50-51 3.3136680938911285E-4 1.0355212793409776E-4 0.0 0.13046532598416977 1.0355212793409776E-4 52-53 3.3136680938911285E-4 1.0355212793409776E-4 0.0 0.15433409147297933 1.1390734072750755E-4 54-55 3.3136680938911285E-4 1.0355212793409776E-4 0.0 0.18115409260791066 1.2426255352091732E-4 56-57 3.5207723497593244E-4 1.0355212793409776E-4 0.0 0.2131206345011666 1.2426255352091732E-4 58-59 3.5207723497593244E-4 1.4497297910773688E-4 0.0 0.24852510704183464 1.2426255352091732E-4 60-61 3.8314287335616175E-4 1.4497297910773688E-4 0.0 0.2875642592729895 1.4497297910773688E-4 62-63 3.934980861495715E-4 1.4497297910773688E-4 0.0 0.33001027651317616 1.4497297910773688E-4 64-65 3.934980861495715E-4 1.4497297910773688E-4 0.0 0.3781413055769448 1.4497297910773688E-4 66-67 4.3491893732321065E-4 1.4497297910773688E-4 0.0 0.4356334470059559 1.4497297910773688E-4 68-69 4.3491893732321065E-4 1.4497297910773688E-4 0.0 0.5000428705809647 1.4497297910773688E-4 70-71 4.452741501166204E-4 1.4497297910773688E-4 0.0 0.5738030513084226 1.4497297910773688E-4 72-73 5.074054268770791E-4 1.4497297910773688E-4 0.0 0.6536520971584054 1.6568340469455642E-4 74-75 5.384710652573083E-4 1.4497297910773688E-4 0.0 0.7430486492039119 1.6568340469455642E-4 76-77 5.384710652573083E-4 1.4497297910773688E-4 0.0 0.847191024267234 1.6568340469455642E-4 78-79 5.384710652573083E-4 1.4497297910773688E-4 0.0 0.9644741643653932 1.6568340469455642E-4 80-81 5.384710652573083E-4 1.4497297910773688E-4 0.0 1.0963892201406402 1.6568340469455642E-4 82-83 5.59181490844128E-4 1.4497297910773688E-4 0.0 1.2397467860526055 1.6568340469455642E-4 84-85 5.695367036375378E-4 1.4497297910773688E-4 0.0 1.3995794955188852 1.6568340469455642E-4 86-87 5.902471292243573E-4 1.6568340469455642E-4 0.0 1.5782586922691708 1.760386174879662E-4 88-89 6.006023420177671E-4 1.6568340469455642E-4 0.0 1.7734648086377387 1.86393830281376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5655 0.0 38.88907 1 GGTATCA 3455 0.0 36.843925 1 TCAACGC 6240 0.0 34.330498 4 ATCAACG 6250 0.0 34.123573 3 CAACGCA 6335 0.0 33.891712 5 AACGCAG 6685 0.0 32.259388 6 TATCAAC 6900 0.0 31.667255 2 GTACATG 11595 0.0 27.978804 1 ACGCAGA 8260 0.0 26.109043 7 TACATGG 12310 0.0 26.007793 2 CGCAGAG 8785 0.0 24.60256 8 ACATGGG 13050 0.0 24.532259 3 GTACCTG 4295 0.0 21.454466 1 CATGGGG 11130 0.0 21.29578 4 GCAGAGT 11495 0.0 18.639221 9 TACCTGG 5045 0.0 17.795177 2 AGAGTAC 10235 0.0 17.081535 10-11 TATAAGC 2330 0.0 16.308807 3 ATGGGGA 7285 0.0 16.235716 5 AGTACAT 8360 0.0 15.966205 2 >>END_MODULE