FastQCFastQC Report
Fri 27 May 2016
SRR940232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4828486
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61100.037.9577671
GGTATCA38450.035.350191
ATCAACG65450.034.0366483
TCAACGC65950.033.77934
CAACGCA69450.032.1446885
TATCAAC72250.031.490952
AACGCAG72600.031.0771086
GTACATG115100.027.0450651
ACGCAGA85900.026.0992457
CGCAGAG92200.024.470448
TACATGG124550.024.1024652
ACATGGG125300.023.1239683
CATGGGG109750.020.471484
TATAACG2351.1339034E-720.2125112
GCAGAGT118700.018.927349
GTATAAC7550.018.2546331
ATAACGC2905.2245014E-818.0168293
GTACCTG43100.017.973491
GCTTATA23950.017.2637751
AGAGTAC107300.017.02045810-11
Produced by FastQC (version 0.11.5)