FastQCFastQC Report
Fri 27 May 2016
SRR940231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164580
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.038.6114581
GTATCAA55450.038.0473251
ATCAACG62300.034.0915343
TCAACGC63000.033.636114
CAACGCA65500.032.3507355
TATCAAC66700.031.0597672
AACGCAG68750.030.8080966
ACGCAGA81100.025.942187
GTACATG102500.024.4766961
CGCAGAG87350.024.1400648
TACATGG109200.022.8005942
ACATGGG115900.022.3019333
GCAGAGT102900.020.21549
CATGGGG97950.019.693964
AGAGTAC95700.017.74288610-11
CAGAGTA98500.016.9009810-11
GAGTACA81550.016.198081
CCTATAA20850.016.1806141
AGTACAT78100.016.0616592
ATTCGCG5050.015.98867380-81
Produced by FastQC (version 0.11.5)