FastQCFastQC Report
Fri 27 May 2016
SRR940231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164580
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.040.419081
GTATCAA57700.039.9460641
TCAACGC64500.034.9103784
ATCAACG64950.034.4494973
CAACGCA67550.033.192675
TATCAAC72450.031.2118952
AACGCAG73400.030.802386
ACGCAGA84350.026.63487
GTACATG104200.024.7194961
CGCAGAG91650.024.513028
TACATGG108500.023.3372152
ACATGGG111900.021.9483363
CATGGGG94700.019.9148224
GCAGAGT112050.019.7534169
AGAGTAC100350.017.84399410-11
GTCCTAC12400.017.2464161
GCTTATA19850.017.237731
CAGAGTA103100.016.90734910-11
AGTACAT79650.016.819572
GAGTACA82750.016.7121771
Produced by FastQC (version 0.11.5)