##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940231_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4164580 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.01030692170639 34.0 33.0 34.0 31.0 34.0 2 33.06986514846635 34.0 33.0 34.0 31.0 34.0 3 33.13083624279039 34.0 33.0 34.0 31.0 34.0 4 36.45713949545932 37.0 37.0 37.0 35.0 37.0 5 36.4322961739239 37.0 37.0 37.0 35.0 37.0 6 36.458411412435346 37.0 37.0 37.0 35.0 37.0 7 36.43212208674104 37.0 37.0 37.0 35.0 37.0 8 36.42757613012597 37.0 37.0 37.0 35.0 37.0 9 38.26970354753661 39.0 39.0 39.0 37.0 39.0 10-11 38.22715940142824 39.0 39.0 39.0 37.0 39.0 12-13 38.188457539535804 39.0 39.0 39.0 37.0 39.0 14-15 39.704788117889436 41.0 40.0 41.0 37.0 41.0 16-17 39.684134294454665 41.0 40.0 41.0 37.0 41.0 18-19 39.645412502581294 41.0 40.0 41.0 37.0 41.0 20-21 39.58095222087221 41.0 40.0 41.0 37.0 41.0 22-23 39.51332943538124 41.0 39.5 41.0 37.0 41.0 24-25 39.44261282050051 41.0 39.0 41.0 36.5 41.0 26-27 39.27508944479395 40.5 39.0 41.0 36.0 41.0 28-29 39.40997699647984 41.0 39.0 41.0 36.5 41.0 30-31 39.31492959674205 41.0 39.0 41.0 36.0 41.0 32-33 39.20372390493159 41.0 39.0 41.0 36.0 41.0 34-35 39.344899005421915 41.0 39.0 41.0 36.0 41.0 36-37 39.327638441331416 41.0 39.0 41.0 36.0 41.0 38-39 39.24697616566377 41.0 39.0 41.0 35.5 41.0 40-41 39.133032022436836 41.0 39.0 41.0 35.0 41.0 42-43 39.03048806842467 41.0 39.0 41.0 35.0 41.0 44-45 38.909724870215 41.0 39.0 41.0 35.0 41.0 46-47 38.751725023891964 40.5 38.5 41.0 35.0 41.0 48-49 38.60468918834552 40.0 38.0 41.0 35.0 41.0 50-51 38.42675960120829 40.0 38.0 41.0 34.5 41.0 52-53 38.24031486968674 40.0 38.0 41.0 34.0 41.0 54-55 38.04515017120574 40.0 37.0 41.0 34.0 41.0 56-57 37.8338443492501 40.0 37.0 41.0 34.0 41.0 58-59 37.5444786749204 39.5 36.0 41.0 33.0 41.0 60-61 37.22618955572951 39.0 36.0 41.0 33.0 41.0 62-63 36.9132796104289 39.0 35.0 40.5 32.5 41.0 64-65 36.55326215368656 38.0 35.0 40.0 32.0 41.0 66-67 36.152270697165136 37.0 35.0 40.0 32.0 41.0 68-69 35.72487477728846 37.0 35.0 39.0 31.0 41.0 70-71 35.325057989040914 36.0 35.0 39.0 31.0 40.5 72-73 34.89213221981568 35.5 34.5 38.5 30.5 40.0 74-75 34.47285980819194 35.0 34.0 37.0 30.0 39.0 76-77 33.12927726205284 34.5 32.5 36.0 28.5 38.5 78-79 33.75536344601376 35.0 34.0 36.0 30.0 38.5 80-81 33.59132565588847 35.0 34.0 36.0 30.0 37.0 82-83 33.34781562606554 35.0 34.0 35.5 30.0 37.0 84-85 33.09764189906305 35.0 34.0 35.0 29.0 36.5 86-87 32.843618444116814 35.0 34.0 35.0 29.0 36.0 88-89 32.61138962392366 35.0 33.0 35.0 29.0 36.0 90-91 32.39047370923359 35.0 33.0 35.0 29.0 35.5 92-93 32.166305845967656 35.0 33.0 35.0 27.5 35.0 94-95 31.935003049527204 35.0 33.0 35.0 27.0 35.0 96-97 31.62548900489365 35.0 33.0 35.0 26.0 35.0 98-99 31.2310427462073 34.0 32.0 35.0 25.0 35.0 100-101 28.79807003347276 32.5 28.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12570356472573252 1101 2 0.07213137459608987 1101 3 0.07682283289545921 1101 4 0.0591347333278307 1101 5 0.06036314485628935 1101 6 0.09129498377135548 1101 7 0.09576608497723527 1101 8 0.05293158567347689 1101 9 0.09050449056265819 1101 10-11 0.08478388402500059 1101 12-13 0.08913240834375102 1101 14-15 0.15403989950059582 1101 16-17 0.15651082787562132 1101 18-19 0.16000391720449159 1101 20-21 0.18158933915136544 1101 22-23 0.12601294058830348 1101 24-25 0.13513705118194252 1101 26-27 0.20412602493635035 1101 28-29 0.16495931554711518 1101 30-31 0.09849352498334696 1101 32-33 0.17188051500832557 1101 34-35 0.17378405711879452 1101 36-37 0.18032071175240816 1101 38-39 0.1512173431080015 1101 40-41 0.23141346977321575 1101 42-43 0.2228869736069541 1101 44-45 0.19406243401145673 1101 46-47 0.22839801409446636 1101 48-49 0.25843985837383343 1101 50-51 0.23158864410378044 1101 52-53 0.25235015680551953 1101 54-55 0.33590844987777047 1101 56-57 0.24943062392016913 1101 58-59 0.2808950865279982 1101 60-61 0.286385186150909 1101 62-63 0.3130252531368001 1101 64-65 0.2897304816533648 1101 66-67 0.23092064952668778 1101 68-69 0.2148061140672226 1101 70-71 0.2445163844609155 1101 72-73 0.27440430852422537 1101 74-75 0.3148897860007125 1101 76-77 0.231987384734488 1101 78-79 0.3395657390096005 1101 80-81 0.29178403657336105 1101 82-83 0.22738101246070386 1101 84-85 0.23627721981854677 1101 86-87 0.26774441650774605 1101 88-89 0.30895085805520495 1101 90-91 0.3366986853516778 1101 92-93 0.30240695685343155 1101 94-95 0.31992309988366685 1101 96-97 0.3272029008673911 1101 98-99 0.22696162540301756 1101 100-101 0.40016622048743145 1102 1 0.05772031415170886 1102 2 0.01915966703046479 1102 3 0.04227808049684967 1102 4 -0.02665510785133307 1102 5 -3.9420053964533963E-4 1102 6 0.0220889433312621 1102 7 -0.010383840213279427 1102 8 -0.017359571259845552 1102 9 -1.3319164074943046E-4 1102 10-11 0.045847281500677184 1102 12-13 0.049387148583996066 1102 14-15 0.09580708743017397 1102 16-17 0.0547584196693407 1102 18-19 0.03132231914204198 1102 20-21 0.09250500197286016 1102 22-23 0.05536021722124218 1102 24-25 0.040935610284080326 1102 26-27 0.15378234908789779 1102 28-29 0.015088707657987754 1102 30-31 0.06356620748504582 1102 32-33 0.16886237396877135 1102 34-35 0.1430662420591915 1102 36-37 0.10463526175586679 1102 38-39 0.09071385906775475 1102 40-41 0.14323878506957755 1102 42-43 0.1421175866581521 1102 44-45 0.17106155915975307 1102 46-47 0.14994286091754105 1102 48-49 0.2095958140752714 1102 50-51 0.2769341507299714 1102 52-53 0.2780316991032947 1102 54-55 0.2611629335449095 1102 56-57 0.2921567903300044 1102 58-59 0.3229615332602904 1102 60-61 0.34760943897039454 1102 62-63 0.2836198199336337 1102 64-65 0.2452209583411573 1102 66-67 0.27681605002618426 1102 68-69 0.2975131146747927 1102 70-71 0.32035970687819315 1102 72-73 0.31963467014767133 1102 74-75 0.4397567886552949 1102 76-77 0.3063935848756998 1102 78-79 0.35204075442136684 1102 80-81 0.3626121196607741 1102 82-83 0.26011682017855975 1102 84-85 0.32273960933044776 1102 86-87 0.3596260610732003 1102 88-89 0.3614969254431273 1102 90-91 0.36945177177315003 1102 92-93 0.396912947939704 1102 94-95 0.25636712599265365 1102 96-97 0.27285480723336164 1102 98-99 0.21476922283161315 1102 100-101 0.39468073619769584 1103 1 0.024255799297485225 1103 2 0.008929978346102985 1103 3 0.019716010645183246 1103 4 -0.015246256648374867 1103 5 0.008702417768290616 1103 6 0.06774398623002043 1103 7 -0.021620267663301718 1103 8 -0.016071758157202964 1103 9 -5.963400620387915E-4 1103 10-11 -0.01766731924823972 1103 12-13 0.05433975938328928 1103 14-15 -0.02998548821222613 1103 16-17 -0.039550590959784415 1103 18-19 0.01508779611405231 1103 20-21 0.013563840380697911 1103 22-23 0.05086086831493475 1103 24-25 -0.023373759364375246 1103 26-27 0.05155034891297561 1103 28-29 -0.02301323007456091 1103 30-31 0.00852945659369908 1103 32-33 -0.04726155491192685 1103 34-35 -0.04381687906582954 1103 36-37 -0.02979014266725244 1103 38-39 0.015760106425048548 1103 40-41 0.00258675739323877 1103 42-43 -0.05179377633036353 1103 44-45 -0.0964617892433921 1103 46-47 -0.07051973547779511 1103 48-49 -0.08014141617434944 1103 50-51 -0.06952620894800532 1103 52-53 0.010656884821557355 1103 54-55 -0.017699857908596073 1103 56-57 -0.05149805605291391 1103 58-59 -0.056655947849357347 1103 60-61 -0.02347492220196301 1103 62-63 -0.19691023160318366 1103 64-65 -0.14631360226238144 1103 66-67 -0.19336985854643984 1103 68-69 -0.1530046087323953 1103 70-71 -0.4095766468244051 1103 72-73 -0.2719915483585069 1103 74-75 -0.13812394998510769 1103 76-77 -0.3203157047627343 1103 78-79 -0.19477566874201813 1103 80-81 -0.08669978471807127 1103 82-83 -0.23183972984552526 1103 84-85 -0.26074847797656986 1103 86-87 -0.20069701312556987 1103 88-89 -0.23763708216268498 1103 90-91 -0.17018855025368396 1103 92-93 -0.18589671432298616 1103 94-95 -0.28418208178937476 1103 96-97 -0.2133011175572186 1103 98-99 -0.21633351851034988 1103 100-101 -0.17048855895829362 1104 1 -0.022732310424657953 1104 2 0.031146041095503563 1104 3 0.009048171446039532 1104 4 0.04207138092981921 1104 5 0.008609631679824759 1104 6 0.024542770305430395 1104 7 0.013528980697145698 1104 8 0.07397336490617334 1104 9 0.038225477552423115 1104 10-11 0.02971357178189038 1104 12-13 0.03815574454171866 1104 14-15 0.10411684019982914 1104 16-17 0.08438558665787355 1104 18-19 0.07432501566761118 1104 20-21 0.15266276203956153 1104 22-23 0.07538633937670625 1104 24-25 0.003937428497181372 1104 26-27 0.08591623446918106 1104 28-29 0.07085188246124119 1104 30-31 0.07293794101142481 1104 32-33 0.11228878649885132 1104 34-35 0.14320855259632737 1104 36-37 0.12922781147370443 1104 38-39 0.13731713841543325 1104 40-41 0.1495834299645864 1104 42-43 0.12361077232868212 1104 44-45 0.12188144258603018 1104 46-47 -0.034156946205833094 1104 48-49 0.10911593789474239 1104 50-51 0.2037786949379523 1104 52-53 0.15716733333624688 1104 54-55 0.1350662969833678 1104 56-57 0.13723718747872482 1104 58-59 0.1973285021740736 1104 60-61 0.12426906252780157 1104 62-63 0.1364496636358723 1104 64-65 0.027268517300207407 1104 66-67 0.09427604478687357 1104 68-69 0.19856467128432342 1104 70-71 0.08392307370100838 1104 72-73 0.05752301032380558 1104 74-75 0.06644409367175541 1104 76-77 -0.041876308984690525 1104 78-79 0.10991595180083635 1104 80-81 0.20682782416669454 1104 82-83 0.14702412452930247 1104 84-85 0.09054093345624636 1104 86-87 0.07742406661294865 1104 88-89 0.04486405254616699 1104 90-91 0.041688646931397955 1104 92-93 0.03758173172475665 1104 94-95 0.14625501226328197 1104 96-97 0.07448517973019975 1104 98-99 0.09483894436470663 1104 100-101 0.10240419214331808 1105 1 -0.04121527508267775 1105 2 -0.06927043487703344 1105 3 -0.0637571298506856 1105 4 -0.05920906656572811 1105 5 -0.03565295413253722 1105 6 -0.03777047657990096 1105 7 -0.03963266435751933 1105 8 -0.04763374162828171 1105 9 -0.07967898469014045 1105 10-11 0.005421523726973021 1105 12-13 -0.01899598441836048 1105 14-15 -0.018195198956028946 1105 16-17 -7.024504639190354E-4 1105 18-19 -0.09381819562148053 1105 20-21 -0.08544339102426335 1105 22-23 -0.02553375978423844 1105 24-25 -0.004820040356108279 1105 26-27 0.09235613403694032 1105 28-29 -0.06278802346299273 1105 30-31 0.019792886345769034 1105 32-33 -0.030651017721851304 1105 34-35 -0.046146424520372875 1105 36-37 -0.043812869939976906 1105 38-39 -0.04037625721131377 1105 40-41 -0.04058679632577622 1105 42-43 -0.2676140242642475 1105 44-45 -0.1829752189954661 1105 46-47 -0.009444322254658744 1105 48-49 -0.07191930873633368 1105 50-51 -0.2652772420058511 1105 52-53 -0.03615509864950894 1105 54-55 -0.029875477743303236 1105 56-57 0.002302563781796607 1105 58-59 -0.09109706355183533 1105 60-61 -0.13166603790443787 1105 62-63 0.021775718810033595 1105 64-65 -0.16886112292353772 1105 66-67 -0.12769423871173302 1105 68-69 -0.16892299716214865 1105 70-71 -0.12864999393183751 1105 72-73 -0.12897295331718084 1105 74-75 -0.12101754502643303 1105 76-77 -0.3326535146800893 1105 78-79 -0.1954884786593709 1105 80-81 -0.13665329711476915 1105 82-83 -0.20363621744882465 1105 84-85 -0.11986314739805692 1105 86-87 -0.23178378998506588 1105 88-89 -0.2593364623279726 1105 90-91 -0.18065549240244394 1105 92-93 -0.23269010275274127 1105 94-95 -0.26900026360755547 1105 96-97 -0.42203665474729846 1105 98-99 -0.360865543303742 1105 100-101 -0.1718583936690372 1106 1 -0.06117443623057994 1106 2 0.028208593820536976 1106 3 0.015157329310461876 1106 4 -0.004184784005019537 1106 5 0.013187553593731138 1106 6 0.00615446823246657 1106 7 -7.259314656877791E-4 1106 8 0.04052464689269186 1106 9 -0.1003782120632053 1106 10-11 -0.030774668326010612 1106 12-13 0.03395710608936753 1106 14-15 0.03847312333977726 1106 16-17 -0.011985563046700065 1106 18-19 -0.04222382476055486 1106 20-21 0.018020185134680844 1106 22-23 -0.14657404675995878 1106 24-25 -0.10006148627222444 1106 26-27 0.05079294419146407 1106 28-29 -0.0591770963106697 1106 30-31 0.030360215264089163 1106 32-33 -0.015066580635753724 1106 34-35 -0.056779702666077014 1106 36-37 -0.09446629157353215 1106 38-39 -0.18227885103029706 1106 40-41 -0.11259121133681305 1106 42-43 -0.02598129799493165 1106 44-45 -0.09732124548553145 1106 46-47 -0.035542004373859015 1106 48-49 0.045495978239387114 1106 50-51 -0.00787768350696183 1106 52-53 -0.04943407215944262 1106 54-55 -0.0829803342554456 1106 56-57 -0.10238755763101182 1106 58-59 -0.13008657790062017 1106 60-61 -0.13546791428412774 1106 62-63 -0.1014193694901877 1106 64-65 -0.03512961078225629 1106 66-67 -0.14861228506934054 1106 68-69 -0.039035579943607956 1106 70-71 0.01703405684079229 1106 72-73 0.021653150435575697 1106 74-75 -0.05459563553416302 1106 76-77 -0.24402934249466313 1106 78-79 -0.03791535503906118 1106 80-81 0.0968980273885407 1106 82-83 0.030375096016960867 1106 84-85 0.07271797842975758 1106 86-87 0.12581996277431529 1106 88-89 0.03121679374266506 1106 90-91 0.07656746565495354 1106 92-93 0.0182351179978113 1106 94-95 -0.0734781885523681 1106 96-97 -0.004326113908447837 1106 98-99 -0.17126123866797727 1106 100-101 -0.27657196982798027 1107 1 0.07178427968134571 1107 2 0.0633606129116231 1107 3 0.0725807300483936 1107 4 -0.0038668285128977686 1107 5 0.031453531471498763 1107 6 0.07527612478548917 1107 7 0.059530811451331545 1107 8 0.07688681343257286 1107 9 0.016550534455006982 1107 10-11 0.04735625955362366 1107 12-13 0.07899473983216154 1107 14-15 0.12008092155451067 1107 16-17 0.1324427706699467 1107 18-19 0.1590088302022039 1107 20-21 0.17150214177478773 1107 22-23 0.11707346787975581 1107 24-25 0.15879249303658582 1107 26-27 0.15201311682115204 1107 28-29 0.1569314036667251 1107 30-31 0.12478353639028938 1107 32-33 0.12834252635651922 1107 34-35 0.07159789872093825 1107 36-37 0.06310817488044762 1107 38-39 0.06343366413032214 1107 40-41 0.10716431676416249 1107 42-43 0.1086705186268162 1107 44-45 0.16434334734469758 1107 46-47 0.13561007074029874 1107 48-49 0.14365834783831843 1107 50-51 0.08423923172522052 1107 52-53 0.17122175650038685 1107 54-55 0.14014173804773122 1107 56-57 0.14955764689251083 1107 58-59 0.0676832124944724 1107 60-61 0.053084852567515384 1107 62-63 0.09036406104809203 1107 64-65 0.03961093851902575 1107 66-67 0.06525578355006445 1107 68-69 0.03852639434868621 1107 70-71 0.006030437947053713 1107 72-73 0.008617466688754405 1107 74-75 -0.1618735482914957 1107 76-77 -0.1682463463808901 1107 78-79 0.026379471771022622 1107 80-81 0.0487863734106071 1107 82-83 0.08591042101956248 1107 84-85 0.09530166411871477 1107 86-87 0.014297688198368519 1107 88-89 0.011698896829038574 1107 90-91 0.05635652896888388 1107 92-93 0.17210616468566542 1107 94-95 0.12527311128113894 1107 96-97 0.10081727074988223 1107 98-99 0.21051312989305515 1107 100-101 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-0.43200336107420156 2316 70-71 -0.32171699544814203 2316 72-73 -0.46258866597828785 2316 74-75 -0.4061010363077102 2316 76-77 -0.42020070952467137 2316 78-79 -0.5563172315024332 2316 80-81 -0.5714078460154539 2316 82-83 -0.6033319149469278 2316 84-85 -0.47086561138592486 2316 86-87 -0.5519327664824161 2316 88-89 -0.4280537671043234 2316 90-91 -0.5235906705677209 2316 92-93 -0.6584843597656302 2316 94-95 -0.5762163363899653 2316 96-97 -0.5931502824955963 2316 98-99 -0.5504316919011636 2316 100-101 -0.768777082069338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 8.0 5 47.0 6 63.0 7 155.0 8 340.0 9 676.0 10 1119.0 11 1791.0 12 2665.0 13 3721.0 14 4394.0 15 5742.0 16 7741.0 17 10804.0 18 10203.0 19 8087.0 20 7911.0 21 8938.0 22 9174.0 23 10322.0 24 12261.0 25 15131.0 26 18892.0 27 23856.0 28 30085.0 29 38540.0 30 48630.0 31 61496.0 32 79516.0 33 106017.0 34 153856.0 35 244867.0 36 483486.0 37 1145822.0 38 1419718.0 39 188488.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.204228946233926 18.316587349907593 13.840792471092314 24.638391232766168 2 16.089266834481812 19.990116467679336 38.086489552294715 25.834127145544137 3 18.85542372633095 23.38713569707074 29.0533244424445 28.70411613415381 4 13.872605567311137 16.450039525008332 35.42528524761481 34.25206966006572 5 15.343802260988676 36.1116527656239 33.7692558179298 14.77528915545762 6 34.16029200845136 34.507653551883436 17.180949526922202 14.151104912743001 7 30.13350026569427 30.756999357195152 20.75620935796114 18.353291019149438 8 27.22116035710684 34.08725489725254 19.831075402561602 18.860509343079013 9 26.846644799715698 15.93173861469824 19.507537374717256 37.7140792108688 10-11 25.74677398441139 25.534255555182035 27.270096864509746 21.448873595896824 12-13 26.372545700297678 22.47756615974868 27.46025468600788 23.689633453945767 14-15 23.565653679362626 24.10345100826494 24.659149301970427 27.67174601040201 16-17 24.12034346800878 27.174168823746932 25.342999774286962 23.362487933957325 18-19 24.17162114787085 26.38682652272258 26.485539958411174 22.956012370995392 20-21 24.51932456849039 25.798320245860317 26.350298122562045 23.332057063087248 22-23 24.532116403164547 25.407811476674404 26.227126281117354 23.832945839043695 24-25 24.436292027545385 25.616228709711542 26.101743875772275 23.845735386970798 26-27 24.174345449980965 25.97927824059183 26.052239138377058 23.794137171050146 28-29 24.193445948047014 25.97855928612755 26.091236431468584 23.736758334356853 30-31 24.29327963273052 25.69478036335791 26.02372176825395 23.98821823565762 32-33 24.163770780266095 25.705938344033225 26.009298052504388 24.120992823196293 34-35 24.553332486505788 25.691963358460416 25.7810963599302 23.97360779510359 36-37 24.33176713676576 25.613202874982218 25.90205778659026 24.152972201661765 38-39 24.271307070580946 25.77289906785318 26.116006896253648 23.83978696531223 40-41 24.499098405343847 25.51592717175417 26.061506296395148 23.923468126506837 42-43 24.44403757401707 25.53527606625398 25.847300328004263 24.17338603172469 44-45 24.36895570125571 25.709479861642215 25.72771706048531 24.193847376616766 46-47 24.399730525438176 25.70542866854425 25.655985777544693 24.238855028472887 48-49 24.53068205216558 25.769994315081362 25.579768653226566 24.119554979526487 50-51 24.232215493519156 25.80633581297514 25.73098607782778 24.23046261567793 52-53 24.665602292861916 25.693185646465416 25.467556488930327 24.17365557174234 54-55 24.45195816144025 25.494728518682113 25.668985815091084 24.38432750478655 56-57 24.374477663351126 25.846414986194883 25.768952183527773 24.010155166926218 58-59 24.531064637820048 25.517481831542373 25.7246462506962 24.226807279941383 60-61 24.495450018639346 25.474444886666177 25.916806686872796 24.11329840782168 62-63 24.510690603360228 25.620601510096424 25.767207334925573 24.10150055161777 64-65 24.423119984007656 25.525211055128565 25.690093090574663 24.36157587028912 66-67 24.292405428943752 25.77566887505952 25.61056167423888 24.321364021757844 68-69 24.12637544047112 25.520863473463677 25.93178175444073 24.420979331624476 70-71 24.14308285589423 25.61704901814829 25.813083192062585 24.426784933894893 72-73 24.346306726804986 25.53854768765741 25.67304128540437 24.442104300133234 74-75 24.18526751545327 25.661001219212775 25.650856120286054 24.502875145047902 76-77 24.304115582516292 25.53092758936117 25.728882986725292 24.436073841397242 78-79 24.123044820846278 25.65237070725019 25.694067589048597 24.530516882854933 80-81 24.074540529897373 25.716242694341325 25.833109221097928 24.376107554663378 82-83 24.177497241305534 25.64037669761311 25.66569749677067 24.516428564310687 84-85 24.092897409588694 25.492063850340653 25.67732992104071 24.73770881902995 86-87 24.15448056206359 25.48324401112159 25.85528048791323 24.50699493890159 88-89 23.981067474047197 25.428584738097175 25.816198948321066 24.77414883953456 90-91 23.92981797273559 25.530665226562476 25.940052781675462 24.599464019026474 92-93 23.990249183009478 25.45048241402239 25.933280648280686 24.625987754687443 94-95 23.90242233310442 25.348366461924133 25.88390666045556 24.865304544515894 96-97 24.03326034406209 25.276098987186817 25.788808797324265 24.901831871426825 98-99 23.71132139262196 25.22510094665099 25.851623310512284 25.211954350214764 100-101 23.60411025172356 25.21678166680094 26.133724982562974 25.04538309891252 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74.0 1 61.0 2 93.5 3 123.0 4 98.0 5 96.5 6 171.0 7 404.5 8 725.0 9 860.5 10 794.5 11 769.0 12 845.0 13 941.5 14 1058.5 15 1114.0 16 1100.5 17 1143.5 18 1233.5 19 1346.0 20 1451.5 21 1645.0 22 1996.5 23 2365.0 24 2837.5 25 3672.0 26 4930.5 27 6902.5 28 9508.5 29 12987.0 30 17919.5 31 23794.0 32 30127.5 33 37094.0 34 44743.0 35 52993.5 36 62236.5 37 71922.5 38 81626.0 39 88444.5 40 94583.0 41 106318.5 42 118819.5 43 131285.5 44 144361.0 45 154233.0 46 161932.5 47 171353.5 48 184530.5 49 192897.5 50 196183.5 51 196725.0 52 191169.5 53 188940.0 54 187804.0 55 178743.5 56 167202.0 57 153585.0 58 137808.5 59 120607.0 60 100169.0 61 78753.0 62 61408.0 63 47577.5 64 35657.0 65 26383.5 66 18692.5 67 13787.0 68 9689.0 69 6248.0 70 4393.5 71 3139.5 72 2272.0 73 1613.0 74 1224.0 75 957.0 76 608.5 77 330.0 78 168.5 79 84.5 80 42.5 81 20.5 82 18.5 83 15.5 84 11.0 85 5.5 86 3.0 87 4.5 88 3.0 89 0.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021490762573897006 2 0.0018489259421118098 3 4.0820442877793195E-4 4 0.0030735392284456054 5 0.009220617685336815 6 0.0010325170845559455 7 4.5622847922239455E-4 8 0.0 9 0.0 10-11 0.0 12-13 2.4012025222231293E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024372205600564764 22-23 6.0030063055578235E-5 24-25 8.284148701669797E-4 26-27 4.922465170557415E-4 28-29 6.363186683891293E-4 30-31 2.0410221438896598E-4 32-33 0.0012486253115560272 34-35 4.0820442877793195E-4 36-37 7.803908197225171E-4 38-39 0.0 40-41 3.4817436572235375E-4 42-43 0.0 44-45 3.601803783334694E-4 46-47 0.004310158527390517 48-49 0.001260631324167143 50-51 0.0 52-53 7.203607566669387E-5 54-55 0.0011645832232782178 56-57 1.2006012611115646E-5 58-59 3.241623405001225E-4 60-61 6.0030063055578235E-5 62-63 2.761382900556599E-4 64-65 0.002101052206945238 66-67 3.121563278890068E-4 68-69 1.2006012611115647E-4 70-71 0.0 72-73 0.0016688357529450748 74-75 1.800901891667347E-4 76-77 1.320661387222721E-4 78-79 0.0 80-81 0.0 82-83 4.682344918335102E-4 84-85 6.963487314447075E-4 86-87 0.0016568297403339594 88-89 2.4012025222231293E-5 90-91 0.001596799677278381 92-93 0.005066537321890803 94-95 0.0 96-97 9.84493034111483E-4 98-99 9.604810088892517E-5 100-101 0.007431721806280585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4164580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.48334165851266 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86579716041773 38.804422999033 2 11.578506773524305 11.69043426686461 3 4.173359062262064 6.320553342114773 4 2.058830006251269 4.157464744895224 5 1.2335839601202567 3.113772026160599 6 0.8099554133771062 2.453355351700698 7 0.5781333125816751 2.0430271080259876 8 0.40948749317097755 1.6537837618110662 9 0.3234731434363059 1.469700469570329 >10 1.780157067072322 16.111290139129146 >50 0.12183962345276608 4.229834177251448 >100 0.06226026682749873 5.80163077844522 >500 0.0034268419184446956 1.210916918742061 >1k 0.0011898755873662875 0.9398139162557667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.4012025222231293E-5 0.0 5 0.0 2.4012025222231293E-5 0.0 2.4012025222231293E-5 0.0 6 0.0 2.4012025222231293E-5 0.0 2.4012025222231293E-5 2.4012025222231293E-5 7 0.0 2.4012025222231293E-5 0.0 2.4012025222231293E-5 2.4012025222231293E-5 8 0.0 2.4012025222231293E-5 0.0 2.4012025222231293E-5 2.4012025222231293E-5 9 0.0 2.4012025222231293E-5 0.0 7.203607566669388E-5 2.4012025222231293E-5 10-11 0.0 4.8024050444462585E-5 0.0 9.604810088892517E-5 2.4012025222231293E-5 12-13 2.4012025222231293E-5 4.8024050444462585E-5 0.0 1.2006012611115647E-4 9.604810088892517E-5 14-15 4.8024050444462585E-5 4.8024050444462585E-5 0.0 1.3206613872227213E-4 1.3206613872227213E-4 16-17 4.8024050444462585E-5 4.8024050444462585E-5 0.0 1.8009018916673468E-4 1.4407215133338777E-4 18-19 4.8024050444462585E-5 4.8024050444462585E-5 0.0 1.9209620177785034E-4 1.6808417655561904E-4 20-21 4.8024050444462585E-5 4.8024050444462585E-5 0.0 1.9209620177785034E-4 2.04102214388966E-4 22-23 4.8024050444462585E-5 4.8024050444462585E-5 0.0 2.521262648334286E-4 2.2811423961119728E-4 24-25 4.8024050444462585E-5 6.0030063055578235E-5 0.0 3.121563278890068E-4 2.521262648334286E-4 26-27 4.8024050444462585E-5 7.203607566669388E-5 0.0 4.4422246661127894E-4 2.761382900556599E-4 28-29 8.404208827780953E-5 7.203607566669388E-5 0.0 9.84493034111483E-4 2.8814430266677554E-4 30-31 9.604810088892517E-5 7.203607566669388E-5 0.0 0.0028814430266677553 3.0015031527789115E-4 32-33 9.604810088892517E-5 7.203607566669388E-5 0.0 0.007899956298114096 3.121563278890068E-4 34-35 9.604810088892517E-5 8.404208827780953E-5 0.0 0.014143082855894232 3.121563278890068E-4 36-37 1.4407215133338777E-4 1.0805411350004082E-4 0.0 0.02482843407978716 3.121563278890068E-4 38-39 1.6808417655561904E-4 1.2006012611115647E-4 0.0 0.04083244889040431 3.121563278890068E-4 40-41 1.6808417655561904E-4 1.3206613872227213E-4 0.0 0.05776092667207737 3.121563278890068E-4 42-43 1.6808417655561904E-4 1.4407215133338777E-4 0.0 0.07753482944258484 3.121563278890068E-4 44-45 1.9209620177785034E-4 1.4407215133338777E-4 0.0 0.10185901099270514 3.121563278890068E-4 46-47 2.04102214388966E-4 1.4407215133338777E-4 0.0 0.13180200644482756 3.121563278890068E-4 48-49 2.1610822700008164E-4 1.4407215133338777E-4 0.0 0.16229727847706132 3.361683531112381E-4 50-51 2.1610822700008164E-4 1.4407215133338777E-4 0.0 0.19417324195957336 3.361683531112381E-4 52-53 2.1610822700008164E-4 1.4407215133338777E-4 0.0 0.227670017144586 3.361683531112381E-4 54-55 2.1610822700008164E-4 1.4407215133338777E-4 0.0 0.2685865081232681 3.361683531112381E-4 56-57 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.3188436769133982 3.361683531112381E-4 58-59 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.3741073529623635 3.361683531112381E-4 60-61 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.43327298310994145 3.601803783334694E-4 62-63 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.4976492227307435 3.841924035557007E-4 64-65 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.5737193186347722 4.2021044138904767E-4 66-67 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.6650850746053624 4.442224666112789E-4 68-69 2.4012025222231294E-4 1.4407215133338777E-4 0.0 0.7657434843369559 4.5622847922239455E-4 70-71 3.121563278890068E-4 1.4407215133338777E-4 0.0 0.8790442253480544 4.5622847922239455E-4 72-73 3.361683531112381E-4 1.4407215133338777E-4 0.0 1.0010613315148227 4.5622847922239455E-4 74-75 3.361683531112381E-4 1.4407215133338777E-4 0.0 1.143320574943932 4.682344918335102E-4 76-77 3.361683531112381E-4 1.4407215133338777E-4 0.0 1.306518304366827 4.802405044446259E-4 78-79 3.4817436572235375E-4 1.4407215133338777E-4 0.0 1.4918191030067858 5.042525296668572E-4 80-81 4.322164540001633E-4 1.4407215133338777E-4 0.0 1.6958732933453073 5.042525296668572E-4 82-83 4.322164540001633E-4 1.4407215133338777E-4 0.0 1.9172521598816687 5.042525296668572E-4 84-85 4.322164540001633E-4 1.4407215133338777E-4 0.0 2.165296380427318 5.042525296668572E-4 86-87 4.322164540001633E-4 1.4407215133338777E-4 0.0 2.439237570175144 5.042525296668572E-4 88-89 4.322164540001633E-4 1.4407215133338777E-4 0.0 2.7459911923891482 5.042525296668572E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3445 0.0 40.41908 1 GTATCAA 5770 0.0 39.946064 1 TCAACGC 6450 0.0 34.910378 4 ATCAACG 6495 0.0 34.449497 3 CAACGCA 6755 0.0 33.19267 5 TATCAAC 7245 0.0 31.211895 2 AACGCAG 7340 0.0 30.80238 6 ACGCAGA 8435 0.0 26.6348 7 GTACATG 10420 0.0 24.719496 1 CGCAGAG 9165 0.0 24.51302 8 TACATGG 10850 0.0 23.337215 2 ACATGGG 11190 0.0 21.948336 3 CATGGGG 9470 0.0 19.914822 4 GCAGAGT 11205 0.0 19.753416 9 AGAGTAC 10035 0.0 17.843994 10-11 GTCCTAC 1240 0.0 17.246416 1 GCTTATA 1985 0.0 17.23773 1 CAGAGTA 10310 0.0 16.907349 10-11 AGTACAT 7965 0.0 16.81957 2 GAGTACA 8275 0.0 16.712177 1 >>END_MODULE