FastQCFastQC Report
Fri 27 May 2016
SRR940230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250036
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53500.038.399381
GGTATCA33300.038.3417241
ATCAACG63100.033.791333
TCAACGC63850.033.320034
CAACGCA66300.032.3028685
AACGCAG70650.030.448736
TATCAAC67700.029.2802622
ACGCAGA82450.025.860927
GTACATG105450.024.6254921
CGCAGAG89400.023.7987618
ACATGGG115050.023.1560543
TACATGG112200.022.8006882
CATGGGG99000.020.6742784
GCAGAGT109200.019.3529019
TATAAGC20550.019.1802643
GCTTATA22100.017.5924761
ATGGGGA61300.016.8103715
AGAGTAC100300.016.76241710-11
TTATAGG21350.016.459713
CCTATAA21000.016.4568771
Produced by FastQC (version 0.11.5)